Full-automatic immunohistochemical dyeing machine

Abstract

The utility model relates to the technical field of immunohistochemistry, and discloses a full-automatic immunohistochemistry dyeing machine which comprises an instrument shell, a translation mechanism and a cover opening mechanism, the translation mechanism comprises a bearing plate, the bearing plate is fixedly installed on the upper middle portion of the interior of the instrument shell through bolts, and the cover opening mechanism comprises a transmission shaft. The transmission shaft is movably installed at the top end of the middle of the right side in the instrument shell through insertion. According to the utility model, the dyeing device for independently controlling each slide is designed and mounted to dye the slides on the four glass carrying discs respectively, so that each slide to be dyed can be controlled to carry out independent heating temperature control and independent dyeing procedures, the dyeing effect of each slide is ensured, and the dyeing efficiency is improved. The rapid staining and freezing staining reagents are used for the to-be-stained slide in the glass slide disc, three staining modes can be carried out, staining results are kept consistent, and the staining cost is reduced and the staining time is shortened while the conventional staining effect is achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of immunohistochemistry, in particular to a full-automatic immunohistochemical staining machine. Background Art

[0002] Immunohistochemistry is an important biological experimental technique used to detect and quantitatively analyze the expression of specific proteins in tissue samples. This technology plays a key role in medical diagnosis, biological research and drug development. With the increasing demand for protein expression at the cellular and tissue levels, immunohistochemistry is also developing. The Chinese patent application number 202321618410.3 discloses "a fully automatic immunohistochemistry staining machine, including a frame and a staining platform. The staining platform is located on the frame and is provided with multiple motion axes to drive the staining reaction platform and the liquid adding device. The device such as the tip placement, output pipeline, tip picker and liquid extraction needle moves back and forth; the tip reagent rack is used to place the tip consumables, and the tip reagent rack automatically adds the tip consumables to the staining platform for testing. The staining platform is equipped with a temperature control module to control the test reagents to the specified temperature. The utility model is a fully automatic immunohistochemical staining machine, which is an integrated staining platform integrating slide preparation, cell morphology and immunocytochemistry, providing a standard staining microenvironment; saving time and manpower, improving work efficiency; reducing manual errors, thereby improving slide preparation quality; reducing pollution and improving slide preparation safety".

[0003] This technical solution only solves the problem of uncontrollable staining quality due to reliance on the proficiency of technicians during manual staining. Traditional immunohistochemical staining requires a lot of manual operations, including manually inserting the slide into the fully automatic immunohistochemical staining machine. There are human errors and inconsistencies in the operation process, and too much manual operation can easily bring external contamination into the fully automatic immunohistochemical staining machine, affecting subsequent detection. In addition, traditional methods usually require a long processing time and are inefficient in high-throughput analysis. These limitations limit the application scope and efficiency of immunohistochemical technology. Utility Model Content

[0004] The purpose of the utility model is to provide a fully automatic immunohistochemical staining machine to solve the above-mentioned deficiencies in the prior art.

[0005] In order to achieve the above-mentioned object, the utility model provides the following technical solutions: a fully automatic immunohistochemical staining machine, comprising an instrument housing;

[0006] A translation mechanism, the translation mechanism comprising a bearing plate, the bearing plate being fixedly mounted at an upper middle position inside the instrument housing by bolts;

[0007] The cover opening mechanism comprises a transmission shaft, and the transmission shaft is installed at the middle top of the right side inside the instrument housing through insertion.

[0008] Furthermore, an instrument cover is movably mounted on the top of the instrument shell through a rotating shaft, a mechanical arm is fixedly mounted on the inner middle of the instrument shell through bolts, a scanning module is slidably mounted on the front end of the mechanical arm, a large container slot door is movably mounted on the front bottom end of the instrument shell through a rotating shaft, four sets of slide staining devices are evenly fixedly mounted on the inner middle of the instrument shell through bolts, syringe doors are movably mounted on the left and right sides of the instrument shell through rotating shafts, the mixing station is fixedly mounted on the inner left middle rear end position of the instrument shell through bolts, and a power switch is electrically connected to the middle of the right front end of the instrument shell.

[0009] Furthermore, the translation mechanism includes a translation frame, a glass slide, a reagent reagent and a translation threaded rod. Four translation boxes are installed on the carrying plate through sliding movement, and the four translation boxes are respectively located directly below four glass slide staining devices. A reagent slot is provided at the left end of the carrying plate. The translation frame is fixedly installed in the middle of the rear side of the four translation boxes by bolts. There are four glass slides and they are respectively installed in the four translation boxes through insertion movement. Twelve glass slide placement slots are provided on each of the glass slides. The reagent reagent is installed in the reagent slot through insertion movement, and the translation threaded rod is installed at the left end of the translation frame through threaded insertion movement.

[0010] Furthermore, the translation mechanism includes a motor, a front panel, a support plate and a spring. The motor is fixedly installed on the inner right side of the instrument housing by bolts. The output shaft of the motor is fixedly installed on the rear end of the translation threaded rod by bolts. Five entrances and exits are opened on the front side of the instrument housing, and are respectively located in front of four glass slides and the reagent box, and a slot is opened in the middle of the bottom end of each entrance and exit. There are five front panels in total and they are respectively installed in the five entrances and exits by insertion. A synchronization shaft is fixedly installed at one end of the front panel located in the instrument housing by insertion, and both ends of the synchronization shaft are respectively installed on both sides of the inside of the instrument housing by insertion. The support plate is installed in the slot in the middle of the bottom end of each entrance and exit by insertion, and the spring is installed in each slot by insertion.

[0011] Furthermore, the cover opening mechanism includes a transmission belt, a lifting threaded rod, a first bevel gear and a second bevel gear. The bottom end of the transmission belt is movably sleeved on the end of the translation threaded rod close to the mechanical arm, and the top end of the transmission belt is movably sleeved on the end of the transmission shaft close to the mechanical arm. The right inner wall of the instrument housing is located above the end of the transmission shaft away from the mechanical arm and is fixed with two mounting blocks by bolts. The two ends of the lifting threaded rod are movably installed between the two mounting blocks by insertion. The first bevel gear is fixedly installed on the end of the transmission shaft away from the mechanical arm by bolts, and the second bevel gear is fixedly installed on the bottom end of the lifting threaded rod by bolts. The second bevel gear is located above the first bevel gear and is meshed with the first bevel gear.

[0012] Furthermore, the cover opening mechanism includes a lifting block, a lifting slot and a support rod. The lifting block is movably connected to the lifting threaded rod through a threaded sleeve. The lifting slot is fixedly installed on the side of the lifting block away from the mechanical arm by bolts. The bottom end of the support rod is movably installed in the lifting slot by insertion, and the top end of the support rod is movably installed on the bottom end of the plane side of the instrument cover through a rotating shaft.

[0013] Compared with the prior art, the fully automatic immunohistochemical staining machine provided by the utility model has

[0014] 1. The utility model is designed and installed with a translation mechanism and a cover opening mechanism. When a glass slide needs to be placed for staining, the glass plate is driven to move out of the instrument housing through the translation frame, thereby preventing the operator's hand from reaching into the instrument housing. While the glass plate moves outward, the motor is driven to rise through the rotation of the lifting threaded rod, and the instrument cover is lifted up through the support plate, so that the instrument cover is lifted up. Through the automatic cover opening, the steps of manually opening the cover to place the glass slide into the instrument housing for staining are reduced, the risk of external contamination entering the instrument housing following the operation of the detection personnel is reduced, and the staining efficiency of the fully automatic immunohistochemical staining machine is improved;

[0015] 2. The utility model uses an independent control device for staining each slide to stain the slides on four glass trays respectively. It can control the independent heating and temperature control of each slide to be stained and the independent staining procedure to ensure the staining effect of each slide. By using rapid staining and frozen staining reagents on the slides to be stained in the glass tray, three staining methods can be performed and the staining results can be kept consistent. While achieving conventional staining effects, the staining cost is reduced and the staining time is shortened. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present utility model. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0017] Figure 1 The overall structural diagram provided for the embodiment of the utility model;

[0018] Figure 2 A schematic diagram of a top cross-sectional structure provided for an embodiment of the utility model;

[0019] Figure 3 A schematic diagram of a partial structure of a side cross-section provided in an embodiment of the utility model;

[0020] Figure 4 A schematic diagram of a cover opening mechanism provided in an embodiment of the utility model.

[0021] Description of reference numerals:

[0022] 1. Instrument housing; 2. Instrument cover; 3. Robotic arm; 4. Scanning module; 5. Large container slot door; 6. Slide staining device; 7. Syringe door; 8. Mixing station; 9. Power switch; 10. Translation mechanism; 101. Loading plate; 102. Translation frame; 103. Glass carrier; 104. Reagent box; 105. Translation threaded rod; 106. Motor; 107. Front panel; 108. Support plate; 109. Spring; 11. Opening mechanism; 111. Transmission shaft; 112. Transmission belt; 113. Lifting threaded rod; 114. First bevel gear; 115. Second bevel gear; 116. Lifting block; 117. Lifting slot; 118. Support rod. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] See also Figure 1-4 , a fully automatic immunohistochemical staining machine, comprising an instrument housing 1;

[0025] The translation mechanism 10 includes a bearing plate 101, which is fixedly mounted at the upper middle of the instrument housing 1 by bolts;

[0026] The cover opening mechanism 11 comprises a transmission shaft 111 , and the transmission shaft 111 is installed at the top middle part of the right side of the inner side of the instrument housing 1 through insertion.

[0027] An instrument cover 2 is movably mounted on the top of the instrument housing 1 through a rotating shaft, a mechanical arm 3 is fixedly mounted in the middle of the interior of the instrument housing 1 through bolts, a scanning module 4 is slidably mounted on the front end of the mechanical arm 3, a large container slot door 5 is movably mounted on the front bottom of the instrument housing 1 through a rotating shaft, four sets of glass slide staining devices 6 are evenly fixedly mounted in the middle of the interior of the instrument housing 1 through bolts, syringe doors 7 are movably mounted on the left and right sides of the instrument housing 1 through rotating shafts, a mixing station 8 is fixedly mounted in the middle and rear end position of the left side of the interior of the instrument housing 1 through bolts, and a power switch 9 is electrically connected in the middle of the right front end of the instrument housing 1; by adopting a four-channel glass slide staining device 6 and independently controlling each glass slide staining device 6, each glass slide to be stained can be controlled to be independently heated and temperature controlled, and an independent staining procedure can be performed to ensure the staining effect of each slide;

[0028] The translation mechanism 10 includes a translation frame 102, a glass slide 103, a reagent box 104 and a translation threaded rod 105. Four translation boxes are installed on the carrier plate 101 through sliding movement, and the four translation boxes are respectively located directly below the four glass slide staining devices 6. A reagent tank is provided at the left end of the carrier plate 101. The translation frame 102 is fixedly installed in the middle of the rear side of the four translation boxes by bolts. There are four glass slides 103 in total and they are respectively installed in the four translation boxes through insertion movement. Each glass slide 103 is provided with twelve glass slide placement slots. The reagent box 104 is installed in the reagent tank through insertion movement, and the translation threaded rod 105 is installed at the left end of the translation frame 102 through threaded insertion movement. By using rapid staining and frozen staining reagents on the glass slides to be stained in the glass slides 103, three staining methods can be performed and the staining results can be kept consistent. While achieving conventional staining effects, the staining cost is reduced and the staining time is shortened.

[0029] The translation mechanism 10 includes a motor 106, a front panel 107, a support plate 108 and a spring 109. The motor 106 is fixedly installed on the right side of the instrument housing 1 by bolts. The output shaft of the motor 106 is fixedly installed on the rear end of the translation threaded rod 105 by bolts. Five entrances and exits are opened on the front side of the instrument housing 1, and are respectively located in front of the four glass slides 103 and the reagent kit 104, and a slot is opened in the middle of the bottom end of each entrance and exit. There are five front panels 107, which are respectively installed in the five entrances and exits by insertion. A synchronization shaft is fixedly installed at one end of the front panel 107 located in the instrument housing 1 by insertion, and The two ends of the synchronization shaft are respectively installed on both sides of the inside of the instrument housing 1 through insertion, the support plate 108 is installed in the slot in the middle of the bottom end of each entrance and exit through insertion, and the spring 109 is installed in each slot through insertion; the translation threaded rod 105 is driven to rotate by the motor 106, and when the translation threaded rod 105 rotates, the translation box is driven to move out of the instrument housing 1 through the translation frame 102, so that the glass carrier 103 moves out of the instrument housing 1 with the translation box, and when the glass carrier 103 moves outward, the front panel 107 is squeezed by the translation box, so that the support plate 108 is pressed into the slot, and the spring 109 is compressed at this time;

[0030] The cover opening mechanism 11 includes a transmission belt 112, a lifting threaded rod 113, a first bevel gear 114 and a second bevel gear 115. The bottom end of the transmission belt 112 is movably sleeved on the end of the translation threaded rod 105 close to the mechanical arm 3, and the top end of the transmission belt 112 is movably sleeved on the end of the transmission shaft 111 close to the mechanical arm 3. The right inner wall of the instrument housing 1 is located above the end of the transmission shaft 111 away from the mechanical arm 3 and is fixed with two mounting blocks by bolts. The two ends of the lifting threaded rod 113 are movably installed between the two mounting blocks by insertion. The first bevel gear 114 is fixedly installed at the end of the transmission shaft 111 away from the mechanical arm 3 by bolts, and the second bevel gear 115 is fixedly installed at the bottom end of the lifting threaded rod 113 by bolts. The second bevel gear 115 is located above the first bevel gear 114 and is meshed with the first bevel gear 114. The transmission shaft 111 is driven to rotate by the transmission belt 112, so that the first bevel gear 114 rotates with the transmission shaft 111. When the first bevel gear 114 rotates, the lifting threaded rod 113 is driven to rotate by the second bevel gear 115.

[0031] The cover opening mechanism 11 includes a lifting block 116, a lifting slot 117 and a support rod 118. The lifting block 116 is movably connected to the lifting threaded rod 113 through a threaded connection. The lifting slot 117 is fixedly installed on the side of the lifting block 116 away from the mechanical arm 3 by bolts. The bottom end of the support rod 118 is movably installed in the lifting slot 117 by insertion, and the top end of the support rod 118 is movably installed on the bottom end of the plane side of the instrument cover 2 through a rotating shaft. The lifting threaded rod 113 is driven to rotate by the second bevel gear 115, so that the lifting block 116 moves upward, and drives the lifting slot 117 to move upward. At this time, the instrument cover 2 is driven to rotate upward and opened by the support rod 118 to automatically complete the cover opening.

[0032] At the same time, the contents not described in detail in this specification belong to the prior art known to technicians in this field.

[0033] Working principle: When in use, the motor 106 is started, and the translation threaded rod 105 is driven to rotate by the motor 106. When the translation threaded rod 105 rotates, the translation frame 102 drives the translation box to move out of the instrument housing 1, so that the glass carrier 103 moves out of the instrument housing 1 following the translation box. When the glass carrier 103 moves outward, the front panel 107 is squeezed by the translation box, so that the support plate 108 is pressed into the slot, and the spring 109 is compressed at this time;

[0034] When the motor 106 is started, the transmission shaft 111 is driven to rotate through the transmission belt 112, so that the first bevel gear 114 rotates along with the transmission shaft 111. When the first bevel gear 114 rotates, the lifting threaded rod 113 is driven to rotate through the second bevel gear 115, so that the lifting block 116 moves upward, and drives the lifting slot 117 to move upward. At this time, the instrument cover 2 is driven to rotate upward and open through the support rod 118, so as to automatically complete the opening of the cover.

[0035] After placing the glass slide into the glass slide placement slot on the glass slide tray 103, the motor 106 is controlled to reverse, and the translation box is driven to move into the instrument housing 1 through the translation frame 102, so that the glass slide is automatically transported to the staining position inside the instrument housing 1, and under the elastic force of the spring 109, the support plate 108 is driven to bounce upward, and the front panel 107 is driven to rotate to close the entrance and exit.

[0036] It should be noted that, in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply that there is any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that the process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. At the same time, in the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate the orientation or position relationship based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the equipment or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present utility model. In the drawings of the present invention, the filling patterns are only used to distinguish the layers and no other limitations are given.

[0037] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A fully automatic immunohistochemical staining machine, comprising an instrument housing (1), characterized in that: A translation mechanism (10), the translation mechanism (10) comprising a bearing plate (101), the bearing plate (101) being fixedly mounted at an upper middle position inside the instrument housing (1); The cover opening mechanism (11) comprises a transmission shaft (111), and the transmission shaft (111) is movably mounted on the top middle part of the right side of the instrument housing (1).

2. The fully automatic immunohistochemical staining machine according to claim 1, characterized in that: An instrument cover (2) is movably mounted on the top of the instrument housing (1) via a rotating shaft, a mechanical arm (3) is fixedly mounted in the middle of the interior of the instrument housing (1) via bolts, a scanning module (4) is slidably mounted on the front end of the mechanical arm (3), a large container slot door (5) is movably mounted on the front bottom of the instrument housing (1) via a rotating shaft, four sets of glass slide staining devices (6) are evenly fixedly mounted in the middle of the interior of the instrument housing (1) via bolts, syringe doors (7) are movably mounted on the left and right sides of the instrument housing (1) via rotating shafts, a mixing station (8) is fixedly mounted at the rear end of the interior of the left middle of the instrument housing (1) via bolts, and a power switch (9) is electrically connected to the middle of the front end of the right side of the instrument housing (1).

3. The fully automatic immunohistochemical staining machine according to claim 2, characterized in that: The translation mechanism (10) comprises a translation frame (102), a glass carrier (103), a reagent box (104) and a translation threaded rod (105). Four translation boxes are mounted on the carrier plate (101) by sliding movement, and the four translation boxes are respectively located directly below four glass slide staining devices (6). A reagent tank is provided at the left end of the carrier plate (101). The translation frame (102) is fixedly mounted at the middle of the rear side of the four translation boxes by bolts. There are four glass carriers (103) and they are respectively mounted in the four translation boxes by insertion movement. Each of the glass carriers (103) is provided with twelve glass slide placement slots. The reagent box (104) is mounted in the reagent tank by insertion movement. The translation threaded rod (105) is mounted at the left end of the translation frame (102) by threaded insertion movement.

4. The fully automatic immunohistochemical staining machine according to claim 3, characterized in that: The translation mechanism (10) comprises a motor (106), a front panel (107), a support plate (108) and a spring (109); the motor (106) is fixedly mounted on the right side of the inside of the instrument housing (1) by means of bolts; the output shaft of the motor (106) is fixedly mounted on the rear end of the translation threaded rod (105) by means of bolts; five entrances and exits are provided on the front side of the instrument housing (1) and are respectively located in front of four glass slides (103) and the reagent box (104); a slot is provided in the middle of the bottom end of each entrance and exit; there are five front panels (107) in total and they are respectively installed in the five entrances and exits by means of insertion; a synchronization shaft is fixedly mounted on one end of the front panel (107) located in the instrument housing (1) by means of insertion; and two ends of the synchronization shaft are respectively installed on two sides of the inside of the instrument housing (1) by means of insertion; the support plate (108) is installed in the slot in the middle of the bottom end of each entrance and exit by means of insertion; and the spring (109) is installed in each slot by means of insertion.

5. The fully automatic immunohistochemical staining machine according to claim 4, characterized in that: The cover opening mechanism (11) comprises a transmission belt (112), a lifting threaded rod (113), a first bevel gear (114) and a second bevel gear (115); the bottom end of the transmission belt (112) is movably sleeved on an end of the translation threaded rod (105) close to the mechanical arm (3); the top end of the transmission belt (112) is movably sleeved on an end of the transmission shaft (111) close to the mechanical arm (3); the right inner wall of the instrument housing (1) is located above an end of the transmission shaft (111) away from the mechanical arm (3) and is fixedly mounted with two mounting blocks by bolts; the two ends of the lifting threaded rod (113) are movably mounted between the two mounting blocks by insertion; the first bevel gear (114) is fixedly mounted on an end of the transmission shaft (111) away from the mechanical arm (3) by bolts; the second bevel gear (115) is fixedly mounted on the bottom end of the lifting threaded rod (113) by bolts; the second bevel gear (115) is located above the first bevel gear (114) and is meshedly connected with the first bevel gear (114).

6. The fully automatic immunohistochemical staining machine according to claim 5, characterized in that: The cover opening mechanism (11) comprises a lifting block (116), a lifting slot (117) and a support rod (118); the lifting block (116) is movably sleeved on the lifting threaded rod (113) via a thread; the lifting slot (117) is fixedly mounted on a side of the lifting block (116) away from the mechanical arm (3) via bolts; the bottom end of the support rod (118) is movably mounted in the lifting slot (117) via insertion; and the top end of the support rod (118) is movably mounted on the bottom end of the plane side of the instrument cover (2) via a rotating shaft.

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