Up-conversion luminoscope

Abstract

The utility model provides an up-conversion luminoscope which is higher in chemical stability of reagents during immunoassay, has no damage to biological tissues and is not interfered by background fluorescence, and comprises an instrument shell as well as an optical module, a conveying module and a control circuit board which are arranged in the instrument shell, the instrument shell is provided with a reagent card access; the optical module comprises an optical seat and first / second circuit boards; the optical base is provided with a first through hole, a second through hole and a third through hole located in the side face, the first circuit board is arranged in the first through hole and is provided with an LED light source; the second circuit board is arranged in the third through hole and is provided with a photodiode; a first plano-convex lens, a first optical filter, a dichroscope and a second plano-convex lens are sequentially arranged between the first through hole and the second through hole from top to bottom, the dichroscope and the third through hole are the same in height, and a second optical filter and a third plano-convex lens are arranged between the dichroscope and the third through hole. The dichroscope is a DM540 / 980 dichroscope, and one surface, facing the LED light source, of the dichroscope is pervious to light; the conveying module conveys the fluorescent reagent card between the reagent card inlet and outlet and the second through hole.

Description

Technical Field

[0001] The utility model relates to the technical field of fluorescent immunoassay, in particular to an up-conversion fluorescence instrument. Background Art

[0002] Fluorescence immunoassay is a method that combines the specificity of immunological reactions with the sensitivity of fluorescence technology. Fluorescence immunoassay plays an important role in basic medical research and clinical diagnosis, and the development of high-performance labeled probes is a decisive factor in the development of this technology. In recent years, semiconductor fluorescent nanocrystals have attracted widespread attention due to their special physical and chemical properties, and have begun to be widely used in optics, biology, medicine and other fields as a new generation of fluorescent markers.

[0003] Currently, down-conversion fluorescence instruments are commonly used on the market. The chemical stability of their reagents is poor, which can easily damage biological tissues, may lead to incorrect detection structures, and are easily interfered by background fluorescence, resulting in low overall detection accuracy. Utility Model Content

[0004] The utility model aims to provide an up-conversion fluorescence instrument to solve the problems existing in the prior art. In the process of immunoassay, the chemical stability of the reagent is higher, no damage is done to biological tissues, and no interference from background fluorescence is caused.

[0005] In order to achieve the above purpose, the solution of the utility model is:

[0006] An upconversion fluorescence instrument comprises an instrument housing, and an optical module, a conveying module and a control circuit board installed in the instrument housing; the instrument housing is provided with a reagent card inlet and outlet; the optical module comprises an optical seat, a first circuit board and a second circuit board; the optical seat is provided with a first through hole and a second through hole opposite to each other up and down, and a third through hole located on the side; the first circuit board is installed in the first through hole, and a side thereof facing the second through hole is provided with an LED light source; the second circuit board is installed in the third through hole and is provided with a photodiode; the first through hole and the second through hole are provided with a plurality of photodiodes in sequence from top to bottom. A first plano-convex lens, a first filter, a dichroic mirror and a second plano-convex lens are provided, the convex surfaces of the first plano-convex lens and the second plano-convex lens are arranged facing each other, the dichroic mirror and the third through hole are arranged at the same height, and the second filter and the third plano-convex lens are arranged between the two; the side of the dichroic mirror facing the LED light source is a light-transmitting surface, and the dichroic mirror is a DM540 / 980 dichroic mirror; the conveying module is used to realize the movement of the fluorescent reagent card between the reagent card inlet and outlet and the bottom of the second through hole; the control circuit board is electrically connected to the first circuit board, the second circuit board and the conveying module.

[0007] The instrument housing comprises an upper cover and a lower shell which cover each other; the reagent card inlet and outlet are arranged on the upper cover; the optical module, the conveying module and the control circuit board are all installed in the lower shell.

[0008] The first filter is a 980nm±22.5 filter; the second filter is a 540nm±10 filter.

[0009] The optical module further includes two fixing seats, which are respectively installed on the first through hole and the third through hole and are used to install and fix the first circuit board and the second circuit board, and the fixing seats are provided with light-transmitting holes.

[0010] Preferably, the up-conversion fluorometer further comprises a heat sink and a cooling fan mounted on the upper surface of the first circuit board, and the cooling fan is electrically connected to the control circuit board.

[0011] The conveying module includes a base plate, a linear guide rail, an inlet and outlet seat and a driving device; the base plate is installed in the instrument housing; the linear guide rail is installed on the base plate, and its two ends are respectively connected to the reagent card inlet and outlet and the bottom of the second through hole; the inlet and outlet seat is slidably fitted on the linear guide rail, and is used for movably clamping and fixing the fluorescent reagent card; the driving device is installed on the base plate, and is used to drive the inlet and outlet seat to slide on the linear guide rail.

[0012] Preferably, the driving device includes a stepper motor and a synchronous belt arranged on the base plate; the synchronous belt is fixed to the base plate through two synchronous wheels, and the stepper motor is transmission connected to one of the synchronous wheels; the side of the entry and exit bin seat is provided with a clip clamped on the synchronous belt.

[0013] Preferably, the conveying module also includes a contact switch; the contact switch is arranged on the side of the linear guide rail, and a contact piece is arranged on the side of the entry and exit bin seat; the contact switch is electrically connected to the control circuit board, and is used to detect the contact piece to determine whether the entry and exit bin seat is moved into place.

[0014] The up-conversion fluorescence instrument further comprises a printer, which is electrically connected to the control circuit board and is used for printing the detection results.

[0015] After adopting the above technical solution, the utility model has the following technical effects:

[0016] Through the above scheme, the utility model utilizes the "anti-Stokes luminescence" principle to realize up-conversion fluorescence analysis, relies on LED light source to emit light, and the point light source becomes parallel light after passing through the first plano-convex lens and is filtered by the first filter to obtain the high-wavelength, low-frequency excitation light source required to excite the fluorescent reagent card. The excitation light source passes through a high-pass dichroic mirror and is focused by the second plano-convex lens onto the card strip of the fluorescent reagent card, so that the fluorescent substance on the card strip obtains photons, and the energy of the particles jumps. After enough particles reach the high energy level, the particle number inversion occurs and photons are released, so that the card strip emits a low-wavelength, high-frequency laser, that is, an emitting light source. The emitting light source passes through the second plano-convex lens. The light is converted into parallel light and reflected by the dichroic mirror to the direction of the third through hole, the reflected emission light source is filtered by the second filter and focused by the third plano-convex lens onto the photodiode, the brightness of the light is associated with the current through the photodiode, the current is then converted into voltage, a corresponding graph is obtained by data analog conversion, and the result is determined by the control circuit board; the utility model realizes the use of a low-energy LED light source as an excitation light source to excite the fluorescent material, and the fluorescent material emits a high-energy emission light source, the chemical stability of the reagent is higher in the process of immunoassay, the low-energy excitation light source is not easy to damage biological tissue, and the up-conversion structure will not be disturbed by background fluorescence. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a three-dimensional diagram of a specific embodiment of the utility model;

[0018] Figure 2 It is a partial structural stereogram of a specific embodiment of the utility model (with the upper cover hidden);

[0019] Figure 3 A cross-sectional view of an optical module according to a specific embodiment of the utility model;

[0020] Figure 4 This is a schematic diagram of an optical module according to a specific embodiment of the utility model;

[0021] Figure 5 A three-dimensional diagram of a conveying module according to a specific embodiment of the utility model;

[0022] Description of Figure Numbers:

[0023] 1-instrument housing; 1a-upper cover; 1b-lower housing; 11-reagent card inlet and outlet;

[0024] 2-optical module; 21-optical seat; 211-first through hole; 212-second through hole; 213-third through hole; 22-first circuit board; 221-LED light source; 23-second circuit board; 231-photodiode; 24-first plano-convex lens; 25-first filter; 26-dichroic mirror; 27-second plano-convex lens; 28-second filter; 29-third plano-convex lens; 210-fixed seat; 2101-light-transmitting hole;

[0025] 3-transport module; 31-bottom plate; 32-linear guide rail; 33-in and out bin seat; 331-clamp; 332-contact piece; 34-driving device; 341-stepping motor; 342-synchronous belt; 343-synchronous wheel; 35-contact switch;

[0026] 4-control circuit board; 5-heat sink; 6-cooling fan; 7-printer; A-fluorescent reagent card. DETAILED DESCRIPTION

[0027] In order to further explain the technical solution of the present utility model, the present utility model is described in detail below through specific embodiments.

[0028] refer to Figures 1 to 5 As shown, the utility model discloses an up-conversion fluorescence instrument, comprising an instrument housing 1, and an optical module 2, a conveying module 3 and a control circuit board 4 installed in the instrument housing 1;

[0029] The instrument housing 1 is provided with a reagent card inlet and outlet 11;

[0030] The optical module 2 includes an optical seat 21, and a first circuit board 22, a second circuit board 23, a first plano-convex lens 24, a first filter 25, a dichroic mirror 26, a second plano-convex lens 27, a second filter 28 and a third plano-convex lens 29 installed on the optical seat 21; the optical seat 21 is provided with a first through hole 211 and a second through hole 212 opposite to each other up and down, and a third through hole 213 located on the side; the first circuit board 22 is installed in the first through hole 211, and a side thereof facing the second through hole 212 is provided with an LED light source 221; the second circuit board 23 is installed in the third through hole 213, and a photodiode 231 is provided; a first plano-convex lens 24, a first filter 25, a dichroic mirror 26 and a second plano-convex lens 27 are sequentially provided between the first through hole 211 and the second through hole 212 from top to bottom, the convex surfaces of the first plano-convex lens 24 and the second plano-convex lens 27 are arranged facing each other, the dichroic mirror 26 is arranged at the same height as the third through hole 213, and a second filter 28 and a third plano-convex lens 29 are arranged between the two; a side of the dichroic mirror 26 facing the LED light source 231 is a light-transmitting side, and the dichroic mirror 26 is a DM540 / 980 dichroic mirror;

[0031] The conveying module 3 is used to realize the movement of the fluorescent reagent card A between the reagent card inlet and outlet 11 and the lower part of the second through hole 212;

[0032] The control circuit board 4 is electrically connected to the first circuit board 22 , the second circuit board 23 and the conveying module 3 to realize the automatic control and fluorescent immunoassay detection functions.

[0033] See also Figure 4 Through the above scheme, the utility model uses the "anti-Stokes luminescence" principle to realize up-conversion fluorescence analysis, relying on the LED light source 221 to emit light, and the point light source is converted into parallel light after passing through the first plano-convex lens 24 and filtered by the first filter 25 to obtain the high-wavelength, low-frequency excitation light source required to excite the fluorescent reagent card A. The excitation light source passes through the high-pass dichroic mirror 26 and is focused by the second plano-convex lens 27 onto the card strip of the fluorescent reagent card A, so that the fluorescent substance on the card strip obtains photons, and the energy of the particles jumps. After enough particles reach the high energy level, the particle number inversion occurs and photons are released, so that the card strip emits a low-wavelength, high-frequency laser, that is, the emission light source. The emission light source passes through the second plano-convex lens 27. It is converted into parallel light and reflected by the dichroic mirror 26 to the direction of the third through hole 213, the reflected emission light source is filtered by the second filter 28 and focused by the third plano-convex lens 29 onto the photodiode 231, the brightness of the light is associated with the current through the photodiode 231, and then the current is converted into voltage, and the data simulation conversion is performed to obtain the corresponding graph, and the result is determined by the control circuit board 4; the utility model realizes the use of a low-energy LED light source as an excitation light source to excite the fluorescent material, and the fluorescent material emits a high-energy emission light source, the chemical stability of the reagent is higher in the process of immunoassay, the low-energy excitation light source is not easy to damage biological tissue, and the up-conversion structure will not be disturbed by the background fluorescence.

[0034] Specific embodiments of the present utility model are shown below.

[0035] The instrument housing 1 comprises an upper cover 1a and a lower shell 1b which cover each other; the reagent card inlet and outlet 11 is arranged on the upper cover 1a; the optical module 2, the transport module 3 and the control circuit board 4 are all installed in the lower shell 1b.

[0036] The first filter 25 is a 980nm±22.5 filter, and the wavelength of the light source after being filtered is approximately between 957.5 and 1002.5nm; the second filter 28 is a 540nm±10 filter.

[0037] The optical module 2 further comprises two fixing seats 210 and 210', which are respectively mounted on the first through hole 211 and the third through hole 213 for mounting and fixing the first circuit board 22 and the second circuit board 23, and light-transmitting holes 2101 and 2101' are provided on the fixing seats 210 and 210'.

[0038] The utility model further includes a heat sink 5 and a heat dissipation fan 6 mounted on the upper surface of the first circuit board 22, and the heat dissipation fan 6 is electrically connected to the control circuit board 4. The heat sink 5 and the heat dissipation fan 6 can dissipate heat for the first circuit board 22 and the optical module 2, thereby extending their service life and improving working stability.

[0039] See also Figure 5 The above-mentioned conveying module 3 includes a base plate 31, a linear guide rail 32, an inlet and outlet bin seat 33 and a driving device 34; the base plate 31 is installed in the instrument housing 1; the linear guide rail 32 is installed on the base plate 31, and its two ends are respectively connected to the reagent card inlet and outlet 11 and the bottom of the second through hole 212; the inlet and outlet bin seat 33 is slidably matched on the linear guide rail 32, and is used for movably clamping and fixing the fluorescent reagent card A; the driving device 34 is installed on the base plate 31, and is used to drive the inlet and outlet bin seat 33 to slide on the linear guide rail 32.

[0040] Furthermore, the above-mentioned driving device 34 includes a stepper motor 341 and a synchronous belt 342 arranged on the base plate 31; the synchronous belt 342 is fixed to the base plate 31 through two synchronous wheels 343, and the stepper motor 341 is transmission connected to one of its synchronous wheels 343; a clip 331 clamped on the synchronous belt 342 is provided on the side of the entry and exit bin seat 33.

[0041] At the same time, the above-mentioned conveying module 33 also includes a contact switch 35; the contact switch 35 is arranged on the side of the linear guide rail 32, and a contact piece 332 is arranged on the side of the entry and exit bin seat 33; the contact switch 35 is electrically connected to the control circuit board 4, and is used to detect the contact piece 332 to determine whether the entry and exit bin seat 33 is moved into place.

[0042] The utility model further comprises a printer 7 , which is electrically connected to the control circuit board 4 and is used for printing the detection result.

[0043] The above embodiments and drawings do not limit the product form and style of the present utility model. Any appropriate changes or modifications made by ordinary technicians in the relevant technical field should be deemed to be within the patent scope of the present utility model.

Claims

1. An upconversion fluorescence instrument, characterized in that: It includes an instrument housing, and an optical module, a conveying module and a control circuit board installed in the instrument housing; The instrument housing is provided with a reagent card inlet and outlet; The optical module comprises an optical seat, a first circuit board and a second circuit board; the optical seat is provided with a first through hole and a second through hole opposite to each other up and down, and a third through hole located on the side; the first circuit board is installed in the first through hole, and a LED light source is provided on a side thereof facing the second through hole; the second circuit board is installed in the third through hole, and a photodiode is provided; a first plano-convex lens, a first filter, a dichroic mirror and a second plano-convex lens are provided in sequence from top to bottom between the first through hole and the second through hole, the convex surfaces of the first plano-convex lens and the second plano-convex lens are arranged facing each other, the dichroic mirror is arranged at the same height as the third through hole, and a second filter and a third plano-convex lens are provided between the two; a side of the dichroic mirror facing the LED light source is a light-transmitting surface, and the dichroic mirror is a DM540 / 980 dichroic mirror; The conveying module is used to realize the movement of the fluorescent reagent card between the reagent card inlet and outlet and below the second through hole; The control circuit board is electrically connected to the first circuit board, the second circuit board and the conveying module.

2. The up-conversion fluorometer according to claim 1, characterized in that: The instrument housing comprises an upper cover and a lower shell which cover each other; the reagent card inlet and outlet are arranged on the upper cover; the optical module, the conveying module and the control circuit board are all installed in the lower shell.

3. The up-conversion fluorometer according to claim 1, characterized in that: The first filter is a 980nm±22.5 filter; the second filter is a 540nm±10 filter.

4. The up-conversion fluorometer according to claim 1, characterized in that: The optical module further includes two fixing seats, which are respectively installed on the first through hole and the third through hole and are used to install and fix the first circuit board and the second circuit board, and the fixing seats are provided with light-transmitting holes.

5. The up-conversion fluorometer according to claim 1, characterized in that: It also includes a heat sink and a heat dissipation fan installed on the upper surface of the first circuit board, and the heat dissipation fan is electrically connected to the control circuit board.

6. The up-conversion fluorometer according to claim 1, characterized in that: The conveying module includes a base plate, a linear guide rail, an inlet and outlet seat and a driving device; the base plate is installed in the instrument housing; the linear guide rail is installed on the base plate, and its two ends are respectively connected to the reagent card inlet and outlet and the bottom of the second through hole; the inlet and outlet seat is slidably fitted on the linear guide rail, and is used for movably clamping and fixing the fluorescent reagent card; the driving device is installed on the base plate, and is used to drive the inlet and outlet seat to slide on the linear guide rail.

7. The up-conversion fluorometer according to claim 6, characterized in that: The driving device includes a stepper motor and a synchronous belt arranged on the base plate; the synchronous belt is fixed to the base plate through two synchronous wheels, and the stepper motor is transmission-connected with one of the synchronous wheels; and a clip clamped on the synchronous belt is arranged on the side of the entry and exit bin seat.

8. The up-conversion fluorometer according to claim 6, characterized in that: The conveying module also includes a contact switch; the contact switch is arranged on the side of the linear guide rail, and a contact piece is arranged on the side of the entry and exit bin seat; the contact switch is electrically connected to the control circuit board and is used to detect the contact piece to determine whether the entry and exit bin seat is moved into place.

9. The up-conversion fluorometer according to claim 1, characterized in that: It also includes a printer, which is electrically connected to the control circuit board and is used to print the test results.

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