Mesenchymal stem cell filter separator
By designing a filter separator for mesenchymal stem cell separation and using a clamping mechanism to stably clamp the centrifuge barrel, the problem of rotational imbalance during high-speed rotation is solved and effective separation of mesenchymal stem cells is achieved.
Patent Information
- Application Number
- CN202421008944.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-10
- Publication Date
- 2025-01-21
- Estimated Expiration
- 2034-05-10
AI Technical Summary
The existing separator cannot stabilize the protection of centrifugal rotors during high-speed rotation, resulting in unbalanced rotor rotation, which in turn causes the separation of mesenchymal stem cells to fail.
A mesenchymal stem cell filtration separator is designed, which uses a clamping mechanism to clamp and fix the high-speed rotating centrifuge barrel to ensure that it remains stable during the rotation process. The equipment includes a base, a fixed frame, a rotating motor, a rotating disc, a moving shaft, a clamping roller and a fixed disc. The rotating motor drives the rotating disc and the moving shaft to rotate, and the clamping roller of the moving shaft fits the centrifugal barrel. , providing limit clamping to ensure the rotation balance of the centrifuge barrel.
The setting of the clamping mechanism ensures that the centrifuge barrel remains balanced during high-speed rotation, avoids separation failure caused by unbalanced rotation, and improves the success rate of separation of mesenchymal stem cells.
Smart Images
Figure CN222369343U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell separation equipment, in particular to a mesenchymal stem cell filtering separator. Background Art
[0002] Mesenchymal stem cells are a type of non-blood cell-derived stem cells with the potential for self-renewal and multidirectional differentiation. They are widely present in various tissues such as bone marrow, fat, umbilical cord blood, placenta, etc. They have great application prospects in tissue engineering, regenerative medicine and immune regulation.
[0003] When separating mesenchymal stem cells through high-speed rotation, the existing separator does not provide stable protection for the high-speed rotating centrifugal rotor, which may lead to the problem of unbalanced rotation of the rotor and failure of mesenchymal stem cell separation.
[0004] For this purpose, the utility model introduces a mesenchymal stem cell filtering separator. Utility Model Content
[0005] In view of the deficiencies of the prior art, the utility model provides a mesenchymal stem cell filtration separator, which has the advantage of being able to clamp and fix a high-speed rotating centrifuge barrel to keep it stable during rotation, thus solving the problems raised in the background technology.
[0006] The utility model provides the following technical solution: a mesenchymal stem cell filtration separator, comprising a base and a connecting frame, a fixing frame fixedly installed on the top of the base, a rotating motor 1 is provided on the base, and an output shaft of the rotating motor 1 passes through the fixing frame, one end of the output shaft of the rotating motor 1 is fixedly connected to a rotating disk, the rotating disk is slidably connected to a moving shaft, the moving shaft is rotatably connected to a clamping roller, a fixed disk is fixedly installed on the top of the fixing frame, the moving shaft passes through the fixed disk and fits with the inner wall of the fixed disk.
[0007] Preferably, the connecting frame is fixedly mounted with a connecting shaft, the connecting shaft is rotatably connected to a covering plate, one end of the covering plate is meshed with an annular rack, the annular rack is fixedly connected to a fixed circular rail, and the fixed circular rail is rotatably connected to the inner wall of the connecting frame.
[0008] Preferably, the output shaft of the rotating motor one is coaxially fixedly connected with the rotating gear one, the rotating gear one is meshed with the rotating gear two, the rotating gear two is coaxially fixedly connected with the linkage shaft, and the linkage shaft is rotatably connected to the top of the base, one end of the linkage shaft is coaxially fixedly connected with the rotating gear three, and the rotating gear three is meshed with the annular rack.
[0009] Preferably, the base is fixedly connected to a connecting frame, a rotating motor 2 is fixedly installed on the top of the connecting frame, an output shaft of the rotating motor 2 passes through the top of the connecting frame, and the output shaft of the rotating motor 2 is fixedly connected to a centrifugal bucket.
[0010] Preferably, the centrifugal barrel is made of stainless steel.
[0011] Preferably, the number of the covering plates is four.
[0012] Preferably, the material of the cover plate is polycarbonate.
[0013] Preferably, the number of the movable shafts is six, and two clamping rollers are rotatably connected to the outer surface of each movable shaft.
[0014] Compared with the prior art, the utility model has the following beneficial effects:
[0015] 1. The mesenchymal stem cell filter separator is provided with a clamping mechanism. When in use, the rotation of an output shaft of a rotating motor drives the rotating disc connected to the end thereof to rotate, and at the same time drives the moving shaft slidably connected inside the rotating disc to rotate, so that the moving shaft is affected by the trajectory of the inner wall of the fixed disc and can only move linearly along its trajectory toward the center of the fixed disc, so that the clamping rollers rotatably connected on the outer surface of the moving shaft abut against the centrifugal bucket, thereby clamping the centrifugal bucket stably, and then when the centrifugal bucket performs centrifugal rotation of the mesenchymal stem cells subsequently, the clamping rollers on the outer surface of the multiple moving shafts can always fit the centrifugal bucket, so that the centrifugal bucket can rotate in a balanced manner.
[0016] 2. The mesenchymal stem cell filter separator is provided with a cover closing mechanism. When in use, after the clamping mechanism completes the limited clamping of the centrifugal bucket, the output shaft of the rotating motor 1 rotates, which drives the rotating gear 1 to rotate, thereby controlling the synchronous rotation of the rotating gear 2, the linkage shaft and the rotating gear 3. The rotation of the rotating gear 3 drives the annular rack meshing with it and the fixed circular rail connected to the annular rack to rotate in an annular manner, and then controls the sealing plate meshing with it to rotate under the action of the annular rack, so that one end of the four sealing plates moves toward the center of the connecting frame and seals the top of the centrifugal bucket. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 This is a schematic diagram of the overall structure of the utility model device;
[0018] Figure 2 For this utility model Figure 1 A side view schematic diagram of the structure;
[0019] Figure 3 For this utility model Figure 1Schematic diagram of some structures;
[0020] Figure 4 For this utility model Figure 1 Enlarged structural diagram at A in the middle.
[0021] In the figure: 1. base; 2. fixed frame; 3. rotating motor 1; 4. rotating disc; 5. moving shaft; 6. clamping roller; 7. fixed disc; 8. rotating gear 1; 9. rotating gear 2; 10. centrifugal barrel; 11. linkage shaft; 12. rotating gear 3; 13. connecting frame; 14. connecting shaft; 15. covering plate; 16. annular rack; 17. fixed circular rail; 18. connecting frame; 19. rotating motor 2. DETAILED DESCRIPTION
[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0023] See also Figure 1-4 A mesenchymal stem cell filtration separator comprises a base 1 and a connecting frame 13. A fixing frame 2 is fixedly installed on the top of the base 1. A rotating motor 3 is provided on the base 1, and the output shaft of the rotating motor 3 passes through the fixing frame 2. A rotating disc 4 is fixedly connected to one end of the output shaft of the rotating motor 3. The rotating disc 4 is slidably connected to a moving shaft 5. The moving shaft 5 is rotatably connected to a clamping roller 6. A fixed disc 7 is fixedly installed on the top of the fixing frame 2. The moving shaft 5 passes through the fixed disc 7 and fits with the inner wall of the fixed disc 7. By driving the moving shaft 5 to move toward the center of the fixed disc 7, the clamping roller 6 rotatably connected on the outer surface of the moving shaft 5 can fit with the centrifugal bucket 10, thereby providing limited clamping during the high-speed rotation of the centrifugal bucket 10, thereby making it rotationally balanced.
[0024] Among them; the connecting frame 13 is fixedly installed with a connecting shaft 14, the connecting shaft 14 is rotatably connected with a sealing plate 15, one end of the sealing plate 15 is meshed with an annular rack 16, the annular rack 16 is fixedly connected with a fixed circular rail 17, the fixed circular rail 17 is rotatably connected to the inner wall of the connecting frame 13, and the annular rack 16 meshed with it is controlled to rotate by rotating gear three 12, so that the four cover plates 15 are moved toward the center of the connecting frame 13, so as to close the hole in the center of the connecting frame, and then the centrifuge bucket 10 is closed together, so as to prevent the sample from splashing out and causing pollution when the centrifuge tube inside the centrifuge bucket 10 bursts.
[0025] Among them; the output shaft of the rotating motor 3 is coaxially fixedly connected with a rotating gear 8, the rotating gear 8 is meshed with a rotating gear 2 9, the rotating gear 2 9 is coaxially fixedly connected with a linkage shaft 11, and the linkage shaft 11 is rotatably connected to the top of the base 1, one end of the linkage shaft 11 is coaxially fixedly connected with a rotating gear 3 12, and the rotating gear 3 12 is meshed with the annular rack 16.
[0026] Among them; the base 1 is fixedly connected to a connecting frame 18, and a rotating motor 19 is fixedly installed on the top of the connecting frame 18. The output shaft of the rotating motor 19 passes through the top of the connecting frame 18, and the output shaft of the rotating motor 19 is fixedly connected to the centrifuge bucket 10.
[0027] Wherein, the centrifugal barrel 10 is made of stainless steel.
[0028] Among them, the number of the covering plates 15 is four.
[0029] The material of the cover plate 15 is polycarbonate, which has high hardness and is transparent, and thus can effectively facilitate the operator to observe the internal situation of the centrifugal bucket 10.
[0030] There are six movable shafts 5 , and two clamping rollers 6 are rotatably connected to the outer surface of each movable shaft 5 .
[0031] Working principle: when in use, first place the centrifuge tubes containing samples and Ficoll or Percoll solutions respectively into the rotor inside the centrifuge bucket 10, and drive the rotating disc 4 connected to its end to rotate by rotating the output shaft of the rotating motor 3, and drive the moving shaft 5 slidably connected inside the rotating disc 4 to rotate, so that the moving shaft 5 is affected by the trajectory of the inner wall of the fixed disc 7 and can only move linearly along its trajectory toward the center of the fixed disc 7, so that the clamping roller 6 rotatably connected on the outer surface of the moving shaft 5 abuts against the centrifuge bucket 10, so as to clamp the centrifuge bucket 10 stably, and then when the centrifuge bucket 10 performs centrifugal rotation on the mesenchymal stem cells in the subsequent centrifugal rotation, the clamping rollers 6 on the outer surface of the multiple moving shafts 5 can always fit The centrifugal bucket 10 can rotate in a balanced manner. At the same time, when the clamping mechanism completes the limited clamping of the centrifugal bucket 10, the output shaft of the rotating motor 3 rotates, which drives the rotating gear 8 to rotate, thereby controlling the rotating gear 2 9, the linkage shaft 11 and the rotating gear 3 12 to rotate synchronously, and the rotation of the rotating gear 3 12 drives the annular rack 16 meshing with it and the fixed circular rail 17 connected to the annular rack 16 to rotate together, and then the sealing plate 15 meshing with it is controlled to rotate under the action of the annular rack 16, so that one end of the four sealing plates 15 moves toward the center of the connecting frame 13, and the top of the centrifugal bucket 10 is sealed, so as to prevent the centrifuge tube from breaking and causing the sample inside to contaminate the laboratory.
[0032] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.
[0033] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A mesenchymal stem cell filtration separator, characterized in that: The invention comprises a base (1) and a connecting frame (13), wherein a fixing frame (2) is fixedly mounted on the top of the base (1), the base (1) is provided with a rotating motor (3), and the output shaft of the rotating motor (3) passes through the fixing frame (2), one end of the output shaft of the rotating motor (3) is fixedly connected to a rotating disk (4), the rotating disk (4) is slidably connected to a moving shaft (5), the moving shaft (5) is rotatably connected to a clamping roller (6), and a fixed disk (7) is fixedly mounted on the top of the fixing frame (2), the moving shaft (5) passes through the fixed disk (7) and fits with the inner wall of the fixed disk (7).
2. A mesenchymal stem cell filtration separator according to claim 1, characterized in that: The connecting frame (13) is fixedly mounted with a connecting shaft (14), the connecting shaft (14) is rotatably connected to a cover plate (15), one end of the cover plate (15) is meshed with an annular rack (16), the annular rack (16) is fixedly connected to a fixed circular rail (17), and the fixed circular rail (17) is rotatably connected to the inner wall of the connecting frame (13).
3. The mesenchymal stem cell filtration separator according to claim 1, characterized in that: The output shaft of the rotating motor 1 (3) is coaxially fixedly connected to a rotating gear 1 (8), the rotating gear 1 (8) is meshed with a rotating gear 2 (9), the rotating gear 2 (9) is coaxially fixedly connected to a linkage shaft (11), and the linkage shaft (11) is rotatably connected to the top of the base (1), one end of the linkage shaft (11) is coaxially fixedly connected to a rotating gear 3 (12), and the rotating gear 3 (12) is meshed with an annular rack (16).
4. The mesenchymal stem cell filtration separator according to claim 1, characterized in that: The base (1) is fixedly connected to a connecting frame (18), a second rotating motor (19) is fixedly mounted on the top of the connecting frame (18), an output shaft of the second rotating motor (19) passes through the top of the connecting frame (18), and the output shaft of the second rotating motor (19) is fixedly connected to a centrifugal bucket (10).
5. The mesenchymal stem cell filtration separator according to claim 4, characterized in that: The centrifugal bucket (10) is made of stainless steel.
6. The mesenchymal stem cell filtration separator according to claim 2, characterized in that: The number of the covering plates (15) is four.
7. The mesenchymal stem cell filtration separator according to claim 2, characterized in that: The material of the cover plate (15) is polycarbonate.
8. The mesenchymal stem cell filtration separator according to claim 1, characterized in that: The number of the movable shafts (5) is six, and the outer surface of each movable shaft (5) is rotatably connected to two clamping rollers (6).