Sample adding device for preventing nucleic acid aerosol pollution

Abstract

The utility model provides a sample adding device for preventing nucleic acid aerosol pollution, which comprises a plurality of negative pressure sample adding boxes, each negative pressure sample adding box is provided with a sealing cover which is detachably connected, a first cavity and a second cavity which are arranged up and down are arranged in each negative pressure sample adding box, a plurality of sample adding plates are arranged in each first cavity, and a plurality of sample adding holes are formed in each second cavity. The first cavity is communicated with the second cavity through the through hole; the negative pressure sample adding box is fixedly arranged on the base, a third cavity is formed in the base, a disinfection box and a vacuumizing mechanism are arranged in the third cavity, liquid for removing aerosol is stored in the disinfection box, an air outlet of the vacuumizing mechanism is communicated with the disinfection box, and an air inlet of the vacuumizing mechanism is communicated with the second cavity and used for extracting the aerosol in the first cavity. And a liquid pipeline communicated with the disinfection box and the second cavity is also arranged in the third cavity. The sample adding device provided by the utility model provides guarantee for effectively preventing aerosol pollution in a PCR laboratory.

Description

Technical Field

[0001] The utility model belongs to the technical field of nucleic acid sample adding devices, in particular to a sample adding device for preventing nucleic acid aerosol contamination. Background Art

[0002] Polymerase chain reaction (PCR) nucleic acid detection has strong specificity and high sensitivity, and plays a vital role in the diagnosis of diseases. However, because the sensitivity of this detection method is too high, it is easily contaminated by nucleic acids in the environment during operation. The most common and difficult to eliminate contamination is the aerosol produced by the small nucleic acid molecules contained in the sample, which evaporates into the environment, causing serious contamination to the experimental results, amplifying the target DNA fragment in the sample without nucleic acid, resulting in false positive test results and misleading clinicians. Nucleic acid aerosol contamination is a type of air pollution that is very easy to occur in PCR laboratories. Aerosol refers to a gaseous dispersion system composed of tiny particles with a diameter of 0.001 to 100 microns, in which solid or liquid particles are suspended in a gas medium. The density of these solid or liquid particles can be slightly different from that of the gas medium, or it can be very different. For PCR laboratories, aerosol contamination containing nucleic acids has always been a distressing problem.

[0003] The friction between air and liquid surface, centrifuge, violent shaking of reaction tube, opening of PCR cover, repeated aspiration of sample by pipette, sample leakage, etc. will all generate nucleic acid aerosol, but more often it is cross-contamination between samples. Since nucleic acid detection technology has ultra-high sensitivity, once nucleic acid contamination occurs in the laboratory, it is easy to cause false positive results, thus affecting the accuracy of the test. The extraction of nucleic acid in the sample processing link and the leakage of amplification products in the detection link will lead to nucleic acid contamination. The splashed amplification products will form aerosols floating in the air, adhere to the surface of objects and the body of the tester, and contaminate the entire laboratory with the flow of personnel and consumables. More seriously, once nucleic acid aerosol contamination is formed, it can cause false positive results in PCR experimental detection, and it is difficult to remove this nucleic acid aerosol contamination under existing conditions, resulting in the inability of the laboratory to carry out the corresponding molecular detection normally. In severe cases, the laboratory contaminated by nucleic acid aerosols even needs to be closed. Therefore, preventing and removing nucleic acid contamination is the focus of the work of molecular detection laboratories.

[0004] The sources of nucleic acid aerosol contamination include positive quality control products, PCR amplification products, strong positive samples to be tested, etc. Nucleic acid contamination is mainly distributed in two places: ① Nucleic acid aerosol contaminants in the air: diffused to every corner of the laboratory, which is more difficult; ② Nucleic acid contaminants on the surface of objects: pipettes, centrifuges, nucleic acid extractors, transfer windows, PCR instruments, disassembled test kits, gun tip boxes, opened consumables and other surfaces.

[0005] If the laboratory is not designed and constructed in accordance with the basic requirements for PCR laboratory design, the use of Type A1, A2, and B1 biosafety cabinets with circulating air for nucleic acid modeling operations in the laboratory can easily cause the formation of nucleic acid aerosols. In routine experimental operations, only conventional disinfectants such as alcohol, iodide, glutaraldehyde, quaternary ammonium salts, etc. cannot effectively degrade nucleic acids.

[0006] At present, it is impossible to prevent the generation of nucleic acid aerosols. However, under the condition of formal physical isolation, it is still very necessary to incorporate some methods to prevent nucleic acid aerosol contamination as much as possible in daily experimental operations. Utility Model Content

[0007] Aiming at the nucleic acid aerosol contamination problem of current nucleic acid adding equipment, the utility model provides a adding device for preventing nucleic acid aerosol contamination, thereby providing a guarantee for effectively preventing aerosol contamination in PCR laboratories.

[0008] To achieve the above purpose, the technical solution of the utility model is:

[0009] A sample adding device for preventing nucleic acid aerosol contamination, comprising:

[0010] A plurality of negative pressure sample loading boxes, each of which is provided with a detachably connected sealing cover, wherein the negative pressure sample loading box has a first cavity and a second cavity arranged in two layers up and down, wherein a plurality of sample loading plates are arranged in the first cavity, and the first cavity is connected with the second cavity through a through hole;

[0011] A base, wherein the negative pressure loading box is fixedly arranged on the base, wherein the base has a third cavity, wherein a disinfection box and a vacuum pumping mechanism are arranged in the third cavity, wherein a liquid for removing aerosols is stored in the disinfection box, wherein an air outlet of the vacuum pumping mechanism is connected to the disinfection box, and an air inlet of the vacuum pumping mechanism is connected to the second cavity for extracting aerosols in the first cavity, wherein a liquid pipeline connecting the disinfection box and the second cavity is also arranged in the third cavity.

[0012] In a preferred embodiment of the utility model, a box door is arranged on the side of the base.

[0013] In a preferred embodiment of the utility model, the disinfection box is provided with a liquid outlet and a liquid filling port, and a first valve is arranged on the liquid outlet.

[0014] In a preferred embodiment of the utility model, a transparent observation port is provided on the side of the disinfection box.

[0015] In a preferred embodiment of the utility model, a waste collection box is also arranged in the first cavity.

[0016] In a preferred embodiment of the utility model, the second cavity is connected to the vacuum pumping mechanism through a first gas pipeline, and the vacuum pumping mechanism is connected to the disinfection box through a second gas pipeline.

[0017] In a preferred embodiment of the utility model, the vacuum pumping mechanism is a mechanical pump.

[0018] In a preferred embodiment of the utility model, a universal wheel is arranged at the bottom of the base.

[0019] In a preferred embodiment of the utility model, the material of the sample adding device is acid and alkali resistant material.

[0020] Due to the adoption of the above technical solution, the utility model has the following advantages and positive effects compared with the prior art:

[0021] The nucleic acid loading device provided by the utility model comprises a negative pressure loading box and a base, wherein the negative pressure loading box has a first cavity and a second cavity connected to each other, a loading plate is arranged in the first cavity, and a vacuum mechanism is arranged in the base, the vacuum mechanism is used to suck the aerosol generated in the negative pressure loading box to prevent the aerosol from contaminating the nucleic acid sample in the negative pressure loading box, and pass the sucked aerosol into a disinfection box arranged in the base, and the disinfection box stores a liquid for removing the aerosol; in addition, a liquid pipeline connecting the disinfection box and the second cavity is arranged, so that the negative pressure loading box can also be disinfected in a liquid state, and the liquid flows into the second cavity, and then flows into the liquid pipeline and enters the disinfection box. Therefore, the utility model not only makes the space for placing the loading plate in a negative pressure state, and immediately releases the sucked aerosol into the disinfection box for removing the aerosol, but also can adopt liquid disinfection, so that the loading device has double insurance to avoid aerosol contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a schematic structural diagram of a sample adding device for preventing nucleic acid aerosol contamination according to an embodiment of the utility model;

[0023] Figure 2 The structure of the sample adding device for preventing nucleic acid aerosol contamination according to the embodiment of the utility model is shown in FIG. Figure 2 ;

[0024] Explanation of the accompanying drawings: 1-negative pressure sample adding box; 2-first cavity; 3-second cavity; 4-sample adding plate; 5-waste collection box; 6-through hole; 7-base; 8-third cavity; 9-disinfection box; 10-vacuum extraction mechanism; 11-liquid pipeline; 12-first gas pipeline; 13-second gas pipeline; 14-liquid outlet; 15-liquid filling port; 16-first valve; 17-second valve; 18-sealing cover; 19-universal wheel. DETAILED DESCRIPTION

[0025] The following is a further detailed description of a sample adding device for preventing nucleic acid aerosol contamination proposed by the present invention in conjunction with the accompanying drawings and specific embodiments. The advantages and features of the present invention will become clearer according to the following description.

[0026] See also Figure 1-2 , a sample adding device for preventing nucleic acid aerosol contamination, comprising:

[0027] A plurality of negative pressure loading boxes 1 are provided, and the negative pressure loading boxes 1 are provided with a detachably connected sealing cover 18. In the present embodiment, the sealing cover is separately provided with the negative pressure loading box, or one side wall is connected by a hinge, and the other side walls are detachably connected, such as by a snap connection, which is not limited here. The negative pressure loading box 1 has a first cavity 2 and a second cavity 3 arranged in two layers up and down, and a plurality of loading plates 4 are provided in the first cavity 2, and the first cavity 2 is connected with the second cavity 3 through a through hole 6.

[0028] A base 7, a negative pressure loading box 1 is fixedly arranged on the base 7, a third cavity 8 is provided in the base 7, a disinfection box 9 and a vacuum mechanism 10 are arranged in the third cavity 8, a liquid for removing aerosol is stored in the disinfection box 9, an air outlet of the vacuum mechanism 10 is connected to the disinfection box 9, an air inlet of the vacuum mechanism 10 is connected to the second cavity 3, and is used to suck the aerosol in the negative pressure loading box 1, a liquid pipeline 11 connecting the second cavity 3 to the disinfection box 9 is also arranged in the third cavity 8, and a second valve 17 is arranged on the liquid pipeline 11 respectively leading to each second cavity 3.

[0029] In this embodiment, there are two negative pressure loading boxes 1. While one is operating, the other can be used for nucleic acid removal. Each negative pressure loading box 1 is equipped with a detachable sealing cover 18. During operation, the sealing cover 18 is opened. When the aerosol in each negative pressure loading box 1 is sucked, the sealing cover 18 seals the negative pressure loading box 1. The vacuum mechanism 10 directly sucks the gas in the second cavity 3. Since the first cavity 2 and the second cavity 3 are connected, the aerosol in the first cavity 2 can be sucked out. Since the vacuum is also connected to the disinfection box 9, the disinfection box 9 stores a liquid for removing aerosols. The aerosol sucked from the negative pressure loading box 1 is carried into the disinfection box 9 and degraded by the liquid. Furthermore, liquid can also be used to directly disinfect the negative pressure loading box 1. The disinfected liquid flows into the second cavity 3 through the through hole 6 and flows into the disinfection box 9 through the liquid pipeline 11.

[0030] The second cavity 3 is connected to the vacuum pumping mechanism 10 through a first gas pipe 12, and the vacuum pumping mechanism 10 is connected to the disinfection box 9 through a second gas pipe 13. To prevent the liquid in the second cavity 3 from affecting the vacuum pumping, the heights of the first gas pipe 12 and the second gas pipe 13 are higher than the liquid pipe 11 leading into the second cavity 3.

[0031] To facilitate observation and maintenance of the disinfection box 9 and the vacuum mechanism 10, a box door detachably connected to the base 7 is provided on the side of the base 7. The side wall of the box door can be hinged to one side wall of the base 7, and the remaining part can be snap-connected. The box door can also be separated from the base 7 as a whole to ensure that the box door can be opened.

[0032] In order to facilitate adding and removing liquid from the disinfection box 9 , the disinfection box 9 is provided with a liquid outlet 14 and a liquid filling port 15 , and a first valve 16 is provided on the liquid outlet 14 .

[0033] In order to facilitate observation of the conditions in the disinfection box 9, a transparent observation port is provided on the side of the disinfection box 9.

[0034] Since there are some tubes containing nucleic acid samples or other small wastes in the routine sample addition process, a waste collection box 5 is set in the first cavity 2, which can facilitate the small biological wastes generated by routine experimental operations to be directly discarded into the waste collection box 5 on the operating table, and the wastes can be collected after the operation.

[0035] Preferably, the vacuum extraction mechanism 10 is a mechanical pump.

[0036] In order to facilitate movement, a universal wheel 19 is provided at the bottom of the base 7. Compared with the existing biosafety cabinet, the sample loading device of this embodiment occupies a small area and is also easy to move. For example, two 128*86mm sample loading plates 4 and a 128*50*50mm waste collection box 5 are provided in each negative pressure sample loading box 1. Since the space in the fang sample loading box is not large, the vacuum pump 10 can also use a small vacuum pump, such as a small mechanical pump, so the overall volume of the sample loading device can be set small, which is convenient for operation.

[0037] Since the liquid involved is for degrading nucleic acid aerosol, the currently available liquids for degrading nucleic acid aerosol, such as 75% ethanol, diluted 84 disinfectant, hypochlorite disinfectant, PCRguard, DNAZap or DNA AWAY, are acidic and alkaline, so the material of the sample adding device is preferably acid-resistant and alkali-resistant material.

[0038] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments. Even if various changes are made to the present invention, if these changes fall within the scope of the claims of the present invention and their equivalents, they still fall within the protection scope of the present invention.

Claims

1. A sample adding device for preventing nucleic acid aerosol contamination, characterized in that: include: A plurality of negative pressure sample loading boxes, each of which is provided with a detachably connected sealing cover, wherein the negative pressure sample loading box has a first cavity and a second cavity arranged in two layers up and down, wherein a plurality of sample loading plates are arranged in the first cavity, and the first cavity is connected with the second cavity through a through hole; A base, wherein the negative pressure loading box is fixedly arranged on the base, wherein the base has a third cavity, wherein a disinfection box and a vacuum pumping mechanism are arranged in the third cavity, wherein a liquid for removing aerosols is stored in the disinfection box, wherein an air outlet of the vacuum pumping mechanism is connected to the disinfection box, and an air inlet of the vacuum pumping mechanism is connected to the second cavity for sucking aerosols in the first cavity, wherein a liquid pipeline connecting the disinfection box and the second cavity is also arranged in the third cavity.

2. The sample adding device for preventing nucleic acid aerosol contamination according to claim 1, characterized in that: A box door is arranged on the side of the base.

3. The sample adding device for preventing nucleic acid aerosol contamination according to claim 2, characterized in that: The disinfection box is provided with a liquid outlet and a liquid filling port, and a first valve is arranged on the liquid outlet.

4. The sample adding device for preventing nucleic acid aerosol contamination according to claim 2, characterized in that: A transparent observation port is provided on the side of the disinfection box.

5. The sample adding device for preventing nucleic acid aerosol contamination according to claim 1, characterized in that: A waste collection box is also arranged in the first cavity.

6. The sample adding device for preventing nucleic acid aerosol contamination according to claim 1, characterized in that: The second cavity is connected to the vacuum pumping mechanism through a first gas pipeline, and the vacuum pumping mechanism is connected to the disinfection box through a second gas pipeline.

7. The sample adding device for preventing nucleic acid aerosol contamination according to claim 1, characterized in that: The vacuum pumping mechanism is a mechanical pump.

8. The sample adding device for preventing nucleic acid aerosol contamination according to claim 1, characterized in that: The bottom of the base is provided with universal wheels.

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