Cell filtering device capable of being continuously operated by one person before flow type sample loading

Abstract

The utility model discloses a single-person continuously operated pre-flow sample loading cell filtering device which comprises an upper platform and a lower platform, the upper platform and the lower platform are fixed through a plurality of supporting columns, a filtering mechanism is arranged on the upper surface of the upper platform and used for fixing and replacing a filter screen, and a test tube rack is movably arranged on the lower platform and used for fixing and replacing the filter screen. A plurality of flow type test tubes are fixedly arranged in the test tube rack. The single-cell suspension filtering device is simple in structure, easy to assemble and convenient in filtering operation, and solves the problems of inconvenience in operation, time and labor consumption and easiness in polluting an experimental sample in the process of filtering a cell sample into a single-cell suspension.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedical experimental instruments, in particular to a flow-type pre-sample cell filtering device which can be continuously operated by a single person. Background Art

[0002] Flow cytometry is a high-tech method that uses a flow cytometer to simultaneously perform multi-parameter, rapid quantitative analysis and sorting of cells or biological particles in a fast linear flow state. When analyzing cells by flow cytometry, the cell sample must pass through the pipeline in a single cell manner. In order to prevent cell adhesion from affecting the test results and even causing the instrument pipeline to be blocked, researchers are usually required to manually filter the cells through a filter with a corresponding aperture before loading the machine to ensure that the sample loaded on the machine is a single cell suspension.

[0003] The current routine operation method in the laboratory is to first cut the large filter mesh purchased centrally into 2 cm 2 -3cm 2 The small filter mesh is uniformly disinfected and sterilized for use. Before going on the machine, one operator manually fixes the small filter mesh on the new flow tube mouth, and another operator uses a pipette to suck the prepared cell sample from the original flow tube containing the sample, and then injects the liquid into the new flow tube in a vertical direction towards the filter mesh. This process requires the cooperation of two experimental operators. Under the premise of a large sample volume, filtering the sample takes up to several hours. In addition, when the experimenter fixes the filter mesh by hand, there is also the problem of hand contamination. Utility Model Content

[0004] The utility model aims to provide a flow-type pre-sampling cell filtering device which can be continuously operated by one person, so as to solve the problems of inconvenient operation, time-consuming and labor-intensive operation, and easy contamination of experimental samples in the process of filtering cell samples into single cell suspensions.

[0005] The technical scheme adopted by the utility model is: a flow cytometry pre-sampling cell filtering device which can be continuously operated by a single person, comprising an upper and lower platform, the upper and lower platforms are fixed by a plurality of pillars, a filtering mechanism is arranged on the upper surface of the platform located on the upper layer, the filtering mechanism is used for fixing and replacing the filter screen, a test tube rack is movably arranged on the platform located on the lower layer, and a plurality of flow test tubes are fixedly arranged in the test tube rack.

[0006] The utility model is also characterized in that:

[0007] The filtering mechanism includes two groups of reel supports, which are relatively arranged on the upper surface of the platform located at the upper layer. A filter port is opened between the two groups of reel supports. A driving reel and a driven reel are respectively arranged on the two groups of reel supports. Gears are arranged at one end of the driving reel and the driven reel. The driving reel and the driven reel are connected to the crawler through the gear winding. A high-pressure sterilized and unused filter screen is wound on the driving reel, and the other end of the driving reel is connected to a rotating handle.

[0008] A base is arranged on the upper surface of the platform at the upper layer, and a dust cover is rotatably hinged on the base. The dust cover is located on the side of the active reel away from the driven reel. A cutting blade is arranged on the bottom of the dust cover close to the active reel through the base, and the cutting edge of the cutting blade is serrated.

[0009] A sliding groove is provided on the upper surface of the platform located at the lower layer along the length direction. The sliding groove is located below the filter port. The sliding groove slides and engages with the test tube rack. A number of grooves are evenly arranged inwardly on both sides of the bottom of the test tube rack. A number of card slots are evenly arranged on both side walls of the sliding groove. The grooves are arranged opposite to the card slots. A spring is fixed in the card slot, and the other end of the spring is connected to the protrusion.

[0010] The test tube rack is provided with a plurality of limiting circular grooves along the length direction, and a flow test tube is sleeved in each limiting circular groove.

[0011] A push handle is provided at the end of the test tube rack.

[0012] The beneficial effects of the utility model are as follows: the utility model is a flow cytometry pre-loading cell filtration device that can be continuously operated by a single person, is stable, lightweight and easy to carry, and can filter a large number of samples by a single person through simple operations, avoiding direct contact between hands and the filter, reducing the possibility of sample contamination, and the filter can be replaced according to the needs of the experimenter, and can be applied to a variety of different experimental samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a schematic diagram of the structure of the flow-type pre-sampling cell filtration device of the utility model which can be continuously operated by one person;

[0014] Figure 2 This is a side view of the flow-type pre-sampling cell filtering device of the utility model which can be continuously operated by one person;

[0015] Figure 3 This is a schematic diagram of the filtering mechanism structure of the flow-type pre-sampling cell filtering device of the utility model which can be continuously operated by one person;

[0016] Figure 4 This is a top view of a test tube rack of a flow-type pre-sampling cell filtration device of the utility model that can be continuously operated by a single person;

[0017] Figure 5The utility model is a schematic diagram of the card slot structure of the flow-type pre-sample cell filtering device which can be continuously operated by one person.

[0018] In the figure, 1. pillar, 2. platform, 3. dust cover, 4. cutting blade, 5. active reel, 6. filter port, 7. driven reel, 8. gear, 9. crawler track, 10. rotating handle, 11. reel bracket, 12. test tube rack, 13. push handle, 14. flow test tube, 15. spring, 16. slot, 17. groove, 18. protrusion. DETAILED DESCRIPTION

[0019] The utility model is described in detail below with reference to the accompanying drawings and specific implementation modes.

[0020] Example 1

[0021] The structure of the flow-type pre-sampling cell filtering device that can be continuously operated by one person is as follows: Figure 1 As shown, it includes an upper and lower platform 2, which are fixed by multiple pillars 1. A filtering mechanism is set on the upper surface of the platform 2 located on the upper layer, and the filtering mechanism is used to fix and replace the filter screen. The platform 2 located on the lower layer is movably set with a test tube rack 12, and a plurality of flow test tubes 14 are fixedly set in the test tube rack 12.

[0022] Example 2

[0023] like Figure 2 and Figure 3 As shown, the filtering mechanism includes two groups of reel supports 11, which are relatively arranged on the upper surface of the platform 2 located at the upper layer, and a filter port 6 is opened between the two groups of reel supports 11. A driving reel 5 and a driven reel 7 are respectively arranged on the two groups of reel supports 11. Gears 8 are arranged at corresponding ends of the driving reel 5 and the driven reel 7. The driving reel 5 and the driven reel 7 are connected to a crawler 9 by winding the gear 8. A high-pressure sterilized and unused filter screen is wound on the driving reel 5, and the other end of the driving reel 5 is connected to a rotating handle 10, and the rotating handle 10 is used to rotate the filter screen on the driving reel 5.

[0024] A base is provided on the upper surface of the platform 2 located at the upper layer, and the base is rotatably hinged to a dust cover 3, which is located on the side of the active reel 5 away from the driven reel 7. The dust cover 3 prevents the filter from being contaminated when not in use. A cutting blade 4 is provided at the bottom of the dust cover 3 near the active reel 5 through the base, and the cutting blade 4 has a sawtooth shape. When the dust cover 3 is rotatably sleeved on the active reel 5, the cutting blade 4 pierces the filter downward.

[0025] like Figure 4As shown, the upper surface of the platform 2 located at the lower layer is provided with a sliding groove along the length direction, the sliding groove is located below the filter port 6, the filter port 6 is used to filter the cell sample, the sliding groove is slidably engaged with the test tube rack 12, a plurality of grooves 17 are evenly arranged inwardly on both sides of the bottom of the test tube rack 12, and a plurality of card grooves 16 are evenly arranged on both side walls of the sliding groove, as shown in FIG. Figure 5 As shown, the groove 17 is arranged opposite to the clamping groove 16, the spring 15 is fixed in the clamping groove 16, and the other end of the spring 15 is connected to the protrusion 18, and the spring 15 allows the protrusion 18 to flexibly retract or extend.

[0026] Example 3

[0027] The test tube rack 12 is provided with a plurality of limiting circular grooves along the length direction, each limiting circular groove is sleeved with a flow test tube 14 , and a push handle 13 is provided at the end of the test tube rack 12 .

[0028] In actual operation, filters of different calibers are used according to different experimental requirements, and the light transmittance and opacity of test tubes and test tube racks can be changed. The caliber of the test tube and the diameter of the filter port can also be changed according to the different samples being processed.

[0029] The working principle of the flow cell filtering device before sample loading in the utility model which can be continuously operated by one person is as follows: four pillars 1 support two upper and lower platforms 2, the upper platform 2 is used to support the filtering mechanism, the dust cover 3 prevents the filter screen on the active scroll 5 from being contaminated, the filter screen is located above the filter port 6, a plurality of flow test tubes 14 are placed on the test tube rack 12, the handle 13 is pushed to push the test tube rack 12 to move forward and backward, the flow test tube 14 is located below the filter port 6, the flow test tube 14 receives the filtered sample, and the filter screen end Fixed on the driven reel 7, the crawler belt 9 drives the driven reel 7 when rotating the active reel 5, and the discarded filter screen is rolled on the driven reel 7. The groove 17 can flexibly clamp the protrusion 18, so that the test tube rack 12 moves forward at an equal distance. When the protrusion 18 is under pressure, it will retract into the built-in clamping groove 16 and clamp the test tube rack 12, making the filtration process more stable and single-person operation can be continuous; the sample is directly filtered into the flow test tube 14 to prevent the sample from being contaminated; there is no need to fix the filter screen by hand, which reduces the risk of hand contamination during operation.

[0030] The working process of the flow cytometry pre-sampling cell filtering device of the utility model which can be continuously operated by one person is as follows: open the dust cover 3, wind the filter screen sterilized by the high-pressure steam sterilizer onto the active reel 5, arrange the marked flow test tubes 14 neatly on the test tube rack 12 according to the sample sequence, turn the rotating handle 10, clamp the filter screen of the active reel 5 with tweezers, cross the filter port 6 and wind it onto the driven reel 7 to fix the discarded filter screen.

[0031] During the experiment, the groove 17 is flexibly snapped onto the protrusion 18 by pushing the handle 13, the flow test tube 14 is facing the filter port 6, the sample to be filtered is sucked with a pipette and pressed against the filter screen covering the filter port 6, and then vertically and uniformly injected into the flow test tube 14 below, the push handle 13 is pushed to compress the spring 15 at the front end of the test tube rack 12, and the protrusion 18 is retracted into the built-in card slot 16 below, and the test tube rack 12 can be pushed forward one grid; the rotating handle 10 is turned, and the crawler 9 drives the gear 8 to rotate, so that the filter screen is replaced to the unused part, and the above steps are repeated until all samples are filtered; the dust cover 3 is closed to facilitate the filter screen packaging, the cutting blade 4 cuts off the contaminated filter screen, and the contaminated filter screen on the driven reel 7 can be discarded.

[0032] The utility model is a flow-type pre-sampling cell filtration device that can be continuously operated by a single person. A plurality of flow test tubes 14 are placed on a test tube rack 12. The flow test tubes 14 are marked and placed on the test tube rack 12 according to the sample sequence, and the test tube rack 12 moves forward and backward. The test tube rack 12 is pushed forward and backward by pushing the handle 13, and the active reel 5 is driven to rotate by turning the handle 10, so that a single person can quickly filter multiple samples without direct contact between the hands and the samples, thereby reducing the experimental time and avoiding contamination of the samples. The utility model has a simple structure and is easy to assemble when in use. It can be disassembled and placed when not in use to save space. It better solves the problems of inconvenient operation, time and labor consumption, and experimental pollution.

Claims

1. A flow cytometry pre-sampling cell filtration device that can be continuously operated by a single person, characterized in that: The device comprises an upper and lower platform (2), wherein the upper and lower platforms (2) are fixed by a plurality of pillars (1); a filtering mechanism is arranged on the upper surface of the platform (2) located on the upper layer, and the filtering mechanism is used to fix and replace the filter screen; a test tube rack (12) is movably arranged on the platform (2) located on the lower layer, and a plurality of flow test tubes (14) are fixedly arranged in the test tube rack (12).

2. The flow cytometry pre-sampling cell filtration device that can be continuously operated by one person according to claim 1, characterized in that: The filtering mechanism comprises two groups of reel supports (11), the two groups of reel supports (11) are arranged on the upper surface of the platform (2) located at the upper layer, a filtering port (6) is provided between the two groups of reel supports (11), a driving reel (5) and a driven reel (7) are respectively arranged on the two groups of reel supports (11), a gear (8) is arranged at one end of each of the driving reel (5) and the driven reel (7), a crawler belt (9) is wound and connected between the driving reel (5) and the driven reel (7) via the gear (8), a filter screen which has been sterilized under high pressure and is not used is wound and arranged on the driving reel (5), and the other end of the driving reel (5) is connected to a rotating handle (10).

3. The flow cytometry pre-sampling cell filtration device that can be continuously operated by one person according to claim 2, characterized in that: A base is arranged on the upper surface of the platform (2) located at the upper layer, and a dust cover (3) is rotatably hingedly connected to the base. The dust cover (3) is located on a side of the active reel (5) away from the driven reel (7). A cutting blade (4) is arranged at the bottom of the dust cover (3) on a side close to the active reel (5) through the base, and the cutting edge of the cutting blade (4) is serrated.

4. The flow cytometry pre-sampling cell filtration device that can be continuously operated by one person according to claim 3, characterized in that: The upper surface of the platform (2) located at the lower layer is provided with a sliding groove along the length direction, the sliding groove is located below the filter port (6), the sliding groove is slidably engaged with the test tube rack (12), a plurality of grooves (17) are evenly arranged inwardly on both sides of the bottom of the test tube rack (12), a plurality of locking grooves (16) are evenly arranged on both side walls of the sliding groove, the grooves (17) and the locking grooves (16) are arranged opposite to each other, a spring (15) is fixed in the locking groove (16), and the other end of the spring (15) is connected to the protrusion (18).

5. The flow cytometry pre-sampling cell filtration device that can be continuously operated by one person according to claim 1, characterized in that: The test tube rack (12) is provided with a plurality of limiting circular grooves along the length direction, and a flow test tube (14) is sleeved in each limiting circular groove.

6. The flow cytometry pre-sampling cell filtration device that can be continuously operated by one person according to claim 1, characterized in that: A push handle (13) is provided at the end of the test tube rack (12).

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