Active substance detection device for cell cryoprotectant

Abstract

The utility model discloses an active substance detection device for a cell cryoprotectant, which comprises a bracket, a syringe is inserted and fixed on the bracket, the end part of the syringe is connected with a liquid inlet pipe and a liquid outlet pipe, the other end of the liquid inlet pipe is connected with a pipe barrel I, and the other end of the pipe barrel I is also connected with a liquid suction pipe; the other end of the liquid outlet pipe is connected with a second pipe barrel, and the other end of the second pipe barrel is further connected with a dropping pipette. According to the active substance detection device for the cell cryoprotectant, after the needle head on the hose is inserted into the detection agent bottle body, a detection agent can be extracted, the extracted detection agent can be pushed out when the injection cylinder is pushed, when the detection agent is sucked, the second pipe barrel is closed, the first pipe barrel is opened, when the detection agent is pushed out, the first pipe barrel is closed, and the second pipe barrel is opened, so that the detection agent is extracted. Continuous liquid pumping and discharging can be achieved without pulling out the needle head, operation is easy, time and labor are saved, a detection agent can be rapidly sucked out, efficient detection is achieved, and therefore practicability is improved.

Description

Technical Field

[0001] The utility model relates to the field of cell cryoprotectant detection, in particular to an active substance detection device for cell cryoprotectants. Background Art

[0002] Cryoprotectants refer to substances that can protect cells from freezing damage. Adding cryoprotectants to cell suspensions can protect cells from solution damage and ice crystal damage. Cryoprotectants combine with water molecules in the solution to produce hydration, weakening the crystallization process of water to increase the viscosity of the solution and thus reduce the formation of ice crystals. At the same time, cryoprotectants can maintain a certain molar concentration inside and outside the cells, reduce the concentration of electrolytes in unfrozen solutions inside and outside the cells, and protect cells from solute damage. They are an indispensable component in cell freezing. Before using cell cryoprotectants, we must test their active substances to see if they meet the requirements of use. Traditional cell cryoprotectant active substance detection devices use a syringe to extract the test agent from the bottle. Each time it is taken out, it is necessary to insert the needle, aspirate, remove the needle and release the liquid. The operation is cumbersome, time-consuming and labor-intensive, which affects the detection efficiency. How to quickly extract the test agent and achieve efficient detection is a technical problem that we urgently need to solve.

[0003] To solve the above problems, we propose an active substance detection device for cell cryoprotectants to improve the shortcomings of the existing technology. Utility Model Content

[0004] The technical problem to be solved by the utility model is to overcome the defects of the prior art and provide an active substance detection device for cell cryoprotectants. In order to solve the above technical problems, the utility model provides the following technical solutions:

[0005] The utility model discloses an active substance detection device for cell cryoprotectant, comprising a bracket, a syringe barrel is inserted and fixed on the bracket, an end portion of the syringe barrel is connected with a liquid inlet pipe and a liquid outlet pipe, the other end of the liquid inlet pipe is connected with a tube barrel 1, an inner plate 1 and a baffle disc 1 are fixed in the tube barrel 1, a flow guide hole 1 is provided at the center of the baffle disc 1, a spring 1 is fixed at the center of a side of the inner plate 1 facing the syringe barrel, and a sealing disc 1 is fixed on the other end of the spring 1 through the flow guide hole 1, a liquid suction pipe is also connected to the other end of the liquid outlet pipe, a tube barrel 2 is connected to the other end, an inner plate 2 and a baffle disc 2 are fixed in the tube barrel 2, a flow guide hole 2 is provided at the center of the baffle disc 2, a spring 2 is fixed at the center of a side of the inner plate 2 facing the syringe barrel, a sealing disc 2 is fixed on the other end of the spring 2 which is attached to the baffle disc 2 and seals the flow guide hole 2, and a dripping pipe is also connected to the other end of the tube barrel 2.

[0006] Preferably, the other end of the pipette is connected to a hose, and the other end of the hose is detachably connected to a needle.

[0007] Preferably, a hanging plate is also fixed on the top of the bracket, and a placement tube with an upper opening is inserted and fixed on the hanging plate.

[0008] Preferably, a through hole is provided at the bottom of the placement tube.

[0009] Preferably, a support platform is fixed on the side of the bracket.

[0010] Compared with the prior art, the beneficial effects achieved by the utility model are:

[0011] The syringe is connected with a liquid inlet tube and a liquid outlet tube, and the liquid inlet tube and the liquid outlet tube are respectively connected with tube one and tube two, and the pipette and the dropper are respectively connected with tube one and tube two, and the sealing disk one and the sealing disk two are respectively used to block tube one and tube two, so that tube one and tube two have the function of unidirectional flow blocking, and after the needle on the hose is inserted into the detection agent bottle, the detection agent can be extracted, and the extracted detection agent can be pushed out when the syringe is pushed, when the detection agent is sucked, tube two is closed and tube one is opened, and when the detection agent is pushed out, tube one is closed and tube two is opened, and continuous extraction and discharge of liquid can be realized without pulling out the needle, and the operation is simple, time-saving and labor-saving, and the detection agent can be quickly extracted to realize efficient detection, thereby improving practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0013] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0014] Figure 2 This is a schematic structural diagram of a cutaway tube in the utility model;

[0015] Figure 3 It is a schematic diagram of the structure of the utility model when the tube is opened;

[0016] Figure 4 It is a structural schematic diagram of two cutaway sections of the tube in the utility model;

[0017] Figure 5 It is a structural schematic diagram of the utility model when the second tube is opened.

[0018] In the figure: 1. bracket; 2. syringe barrel; 3. liquid inlet pipe; 4. liquid outlet pipe; 5. tube barrel 1; 51. inner plate 1; 52. baffle disc 1; 53. guide hole 1; 54. spring 1; 55. sealing disc 1; 6. pipette; 7. hose; 8. tube barrel 2; 81. inner plate 2; 82. baffle disc 2; 83. guide hole 2; 84. spring 2; 85. sealing disc 2; 9. dropper; 10. needle; 11. hanging plate; 12. placement tube; 13. support table. DETAILED DESCRIPTION

[0019] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention. Example

[0020] like Figure 1-5 As shown, an active substance detection device for cell cryoprotectants comprises a bracket 1, a syringe 2 is inserted and fixed on the bracket 1, the end of the syringe 2 is connected to a liquid inlet pipe 3 and a liquid outlet pipe 4, the other end of the liquid inlet pipe 3 is connected to a tube 5, an inner plate 51 and a baffle plate 52 are fixed in the tube 5, a flow guide hole 53 is opened at the center of the baffle plate 52, a spring 54 is fixed at the center of the side of the inner plate 51 facing the syringe 2, and the other end of the spring 54 passes through the flow guide hole 53 and is fixed with a sealing disk 55, and the other end of the tube 5 is also connected to a liquid suction pipe Tube 6, the other end of the liquid outlet tube 4 is connected to a tube barrel 8, an inner plate 81 and a baffle plate 82 are fixed in the tube barrel 8, a guide hole 83 is opened at the center of the baffle plate 82, a spring 84 is fixed at the center of the side of the inner plate 81 facing the injection cylinder 2, and a sealing disk 85 is fixed on the other end of the spring 84, which is attached to the baffle plate 82 and seals the guide hole 83. The other end of the tube barrel 8 is also connected to a dropper 9, which constitutes an integral structure of the active substance detection device for cell cryoprotectant, which is convenient for rapid and continuous extraction of the detection agent, and helps to improve the detection efficiency.

[0021] In this embodiment, the other end of the pipette 6 is connected to a hose 7, and the other end of the hose 7 is detachably connected to a needle 10, which is convenient for inserting into the bottle of the detection agent. A hanging plate 11 is also fixed to the top of the bracket 1, and a placement tube 12 with an upper opening is inserted and fixed on the hanging plate 11. A through hole is opened at the bottom of the placement tube 12, which is convenient for inserting the needle into the inverted bottle of the detection agent. A support platform 13 is also fixed on the side of the bracket 1, and the container can be placed under the dropper 9 to receive the detection agent withdrawn from the injection cylinder 2.

[0022] The principle and advantages of the utility model are as follows: the detection agent bottle is inserted upside down into the placement cylinder 12, the needle 10 is inserted into the bottle through the through hole at the bottom of the placement cylinder 12, and then the container is placed under the dropper 9, and the stem cell cryoprotectant sample to be tested is poured into the container, and then the rod of the injection cylinder 2 is pulled outward. Figure 3 and Figure 4 As shown, the syringe 2 inhales air to form negative air pressure, and the sealing disk 55 is pulled away from the baffle disk 52, the spring 54 is extended, the tube 5 is opened, the sealing disk 85 is attached to the baffle disk 82, and the guide hole 83 is blocked. At this time, the tube 8 is closed, and no air enters the syringe 2 through the liquid outlet pipe 4. At this time, the detection agent in the detection agent bottle is sucked into the syringe 2 through the needle 10, the hose 7, the liquid pipette 6, the tube 5 and the liquid inlet pipe 3. After the liquid is sucked, the spring 54 contracts by its own elastic force, as shown in FIG. Figure 2 The automatic plugging of the guide hole 53, and then pushing the rod on the syringe 2, the pressure of the detection agent in the syringe 2 increases, such as Figure 5 As shown, the sealing disk 2 85 is flushed, the spring 2 84 is compressed, the guide hole 2 83 is opened, and the detection agent in the syringe 2 is squeezed out through the liquid outlet tube 4, the tube 2 8 and the drip tube 9, and sprayed into the container to mix with the stem cell cryoprotectant sample, thereby completing the extraction and dripping. Continuous extraction and discharge can be achieved without pulling out the needle, which is simple to operate, saves time and effort, can quickly absorb the detection agent, achieve efficient detection, and thus improve practicality.

[0023] Finally, it should be noted that the above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Although the utility model is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions recorded in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A device for detecting active substances in cell cryoprotectants, comprising a support (1), characterized in that: A syringe (2) is inserted and fixed on the bracket (1); the end of the syringe (2) is connected to a liquid inlet pipe (3) and a liquid outlet pipe (4); the other end of the liquid inlet pipe (3) is connected to a tube (5); an inner plate (51) and a flow blocking disk (52) are fixed inside the tube (5); a flow guide hole (53) is provided at the center of the flow blocking disk (52); a spring (54) is fixed at the center of a side of the inner plate (51) facing the syringe (2); and the other end of the spring (54) passes through the flow guide hole (53) and is fixed to a sealing disk (55); the tube (51) is provided with a plurality of sealing plates, each of which is provided with a plurality of sealing plates. The other end of the injection tube (5) is also connected to a liquid suction tube (6), and the other end of the liquid outlet tube (4) is connected to a second tube (8), and an inner plate (81) and a baffle plate (82) are fixed inside the second tube (8), and a second flow guide hole (83) is opened at the center of the second baffle plate (82). A second spring (84) is fixed at the center of the side of the second inner plate (81) facing the injection tube (2), and a sealing plate (85) attached to the second baffle plate (82) and sealingly covering the second flow guide hole (83) is fixed at the other end of the second spring (84). The other end of the second tube (8) is also connected to a drip tube (9).

2. The active substance detection device for cell cryoprotectants according to claim 1, characterized in that: The other end of the liquid pipette (6) is connected to a hose (7), and the other end of the hose (7) is detachably connected to a needle (10).

3. The active substance detection device for cell cryoprotectants according to claim 1, characterized in that: A hanging plate (11) is also fixed to the top of the bracket (1), and a placement tube (12) with an upper opening is inserted and fixed on the hanging plate (11).

4. The active substance detection device for cell cryoprotectants according to claim 3, characterized in that: A through hole is provided at the bottom of the placement cylinder (12).

5. The active substance detection device for cell cryoprotectants according to claim 1, characterized in that: A support platform (13) is also fixed on the side of the bracket (1).

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