Egg examination and transplantation device

Abstract

The utility model relates to an ovum examination and transplantation device, which comprises a rubber tube, a suction nozzle, a filter element gun head, a needle body fixer, a glass needle and a cotton plug, the suction nozzle is connected with one end of the rubber tube, and the needle body fixer is connected with the other end of the rubber tube. The filter element gun head is connected to the suction nozzle, the glass needle is arranged on the needle body fixer, and the cotton plug is arranged in the rubber tube and is in contact with the end part of the glass needle. When the device is used for working, fertilized eggs are sucked into or moved out of the glass needle through oral cavity suction force in the egg checking and transplanting process, so that egg checking and transplanting operation is achieved, the problem that cross infection is caused by the oral cavity and the suction nozzle can be well solved through the filter element gun head, and meanwhile when the fertilized eggs are sucked, the filter element gun head can be used for sucking the fertilized eggs. And if part of liquid enters the rubber tube from the glass needle due to too high suction force, the liquid can be adsorbed by the cotton plug, so that the sucked liquid is prevented from being in direct contact with the rubber tube, and the sanitation problem can be well solved.

Description

Technical Field

[0001] The utility model relates to the technical field of mouse embryo transplantation, in particular to an egg detection and transplantation device. Background Art

[0002] Mouse IVF-ET technology is widely used in the fields of mouse embryo purification, cryopreservation and thawing, rapid propagation, and transgenic mouse preparation. The pipette is an essential experimental tool for egg detection (egg washing, egg transfer) and embryo transfer during IVF-ET. Currently, mouth pipettes or hand-controlled pipettes are used.

[0003] Among them, the traditional oral straw is composed of a hose connected to the nozzle and a glass needle. The oral cavity is in direct contact with the nozzle, which can easily cause cross contamination.

[0004] In the early days, the hand-controlled tube mainly used a single-side sealed hose and a glass needle. Later, it was improved to use a hose with a suction tip and a glass needle, or a suction tip and a glass needle. The early hand-controlled tube was more hygienic than the traditional oral straw, but it could only be used for other operations except embryo transfer, and the use scenarios were limited. In addition, the improved hand-controlled tube had a short pipette length, the liquid rose quickly, and it was easy to contact with the suction tip or hose. Summary of the invention

[0005] In view of the above problems, the utility model provides an egg detection and transplantation device, which can avoid cross infection between the oral cavity and the suction nozzle, and can prevent liquid from contacting the rubber hose by providing a cotton plug, thus having the advantage of high practicality.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] An egg detection and transplantation device, comprising:

[0008] Rubber hose;

[0009] A suction nozzle connected to one end of the rubber tube;

[0010] A filter cartridge gun head, the filter cartridge gun head being connected to the suction nozzle;

[0011] A needle holder, the needle holder being connected to the other end of the rubber tube;

[0012] a glass needle, wherein the glass needle is disposed on the needle holder; and

[0013] A cotton plug is arranged inside the rubber tube and contacts the end of the glass needle.

[0014] In one embodiment, a filter is connected to the middle of the rubber tube.

[0015] In one embodiment, the glass needle comprises:

[0016] a holding section, the holding section being used for being mounted and connected with the needle holder; and

[0017] A capillary segment, one end of which is connected to the holding segment, and the other end of which is a needle tip, and the needle tip is arranged in a conical transition.

[0018] In one embodiment, the needle tip has a rounded and smooth shape.

[0019] In one embodiment, the inner diameter of the needle opening is 100-120 um, and the inner diameter of the capillary section is 200 um-300 um.

[0020] In one embodiment, the glass needle comprises:

[0021] a holding section, the holding section being used for being mounted and connected with the needle holder; and

[0022] A capillary segment, one end of which is connected to the holding segment, and the other end of which is a needle tip, wherein the needle tip and the capillary segment are arranged in a deflected manner, and the deflection angle is 15-30 degrees.

[0023] In one embodiment, the inner diameter of the capillary segment is 100-120 um.

[0024] In one embodiment, the deflection length of the needle tip is 1 mm.

[0025] In one embodiment, the needle tip has a rounded and smooth shape.

[0026] The utility model adopts the above technical solution, which has the following advantages:

[0027] 1. When using the device for work, install the glass needle into the needle holder, insert the filter cartridge gun head into the nozzle, then hold the filter cartridge gun head with the mouth, and hold the needle holder with the right hand at the same time. During the egg detection and transplantation process, the fertilized egg is sucked into or removed from the glass needle by using the oral suction force, so as to realize the egg detection and transplantation operation. Compared with the prior art, the device can well solve the problem of cross infection caused by the oral cavity and the nozzle through the filter cartridge gun head. At the same time, when the fertilized egg is inhaled, if the suction force is too high, causing part of the liquid to enter the rubber tube from the glass needle, the liquid can be absorbed by the cotton plug to avoid the inhaled liquid from directly contacting the rubber tube, which can well solve the hygiene problem and has the advantage of high practicality.

[0028] 2. The filter is mainly used to filter the air in the rubber hose. During the filtering process, it can slow down the flow rate of the gas to prevent the liquid from rising too quickly. At the same time, it is convenient for temporary fixation on the eyepiece tube of the microscope. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is a schematic diagram of the specific structure of the egg detection and transplantation device in one embodiment of the utility model;

[0030] Figure 2 This is a schematic diagram of the structure of a glass needle used for egg detection in one embodiment of the utility model;

[0031] Figure 3 This is a schematic diagram of the needle head structure of a glass needle in one embodiment of the utility model;

[0032] Figure 4 This is a schematic diagram of the structure of a glass needle used for transplantation operation in one embodiment of the utility model;

[0033] Figure 5 This is a schematic diagram of the deflection structure of the needle in one embodiment of the utility model;

[0034] The marks in the figure are as follows:

[0035] 1. Filter gun tip; 2. Suction nozzle; 3. Upper rubber tube; 4. Filter; 5. Lower rubber tube; 6. Cotton plug; 7. Needle holder; 8. Glass needle; 9. Holding section; 10. Capillary section; 11. Needle tip. DETAILED DESCRIPTION

[0036] In order to make the purpose, technical solution and advantages of the utility model clearer, the technical solution of the utility model is described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the utility model, not all of them. Based on the embodiments of the utility model, all other embodiments obtained by ordinary persons in the field without creative work are within the scope of protection of the utility model.

[0037] Unless otherwise defined, the technical or scientific terms used in the present invention shall have the usual meanings understood by persons with ordinary skills in the field to which the present invention belongs. The words "first", "second", "third", "fourth" and similar terms used in the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. Words such as "include" or "comprise" and the like mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, without excluding other elements or objects. Words such as "connect" or "connected" and the like are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect.

[0038] The straw is an indispensable experimental tool for egg detection (egg washing, egg transfer) and embryo transfer in the process of in vitro fertilization-embryo transfer. Currently, oral straws or hand-controlled straws are used. Among them, the traditional oral straw is composed of a hose connected to the nozzle and a glass needle. The oral cavity and the nozzle are in direct contact, which is easy to cause cross contamination. The hand-controlled tube is more hygienic than the traditional oral straw, but it can only be used for other operation processes except embryo transfer, and the use scenarios are limited. In addition, the improved hand-controlled tube has a short pipeline length, the liquid rises quickly, and it is easy to contact with the suction head or hose. In view of the above problems, the utility model provides an egg detection and transplantation device, which can avoid cross infection caused by the oral cavity and the nozzle. At the same time, by setting a cotton plug, it can prevent the liquid from contacting the rubber hose, and has the advantage of high practicality. The specific scheme is as follows.

[0039] Reference Figure 1 As shown, the utility model relates to an egg detection and transplantation device, comprising a rubber tube, a suction nozzle 2, a filter cartridge gun head 1, a needle body holder 7, a glass needle 8 and a cotton plug 6, wherein the suction nozzle 2 is connected to one end of the rubber tube, and the needle body holder 7 is connected to the other end of the rubber tube. The filter cartridge gun head 1 is connected to the suction nozzle 2, the glass needle 8 is arranged on the needle body holder 7, and the cotton plug 6 is arranged inside the rubber tube, and the cotton plug 6 is in contact with the end of the glass needle 8.

[0040] It should be noted that, in this embodiment, the filter cartridge gun head 1 is a disposable item, and the filter cartridge gun head 1 is directly inserted into and connected with the suction nozzle 2. Except for the connection between the filter cartridge gun head 1 and the suction nozzle 2, the interfaces between other components are all connected by rubber hoses and plastic or rubber, and the connection is tight and not easy to fall off.

[0041] Illustratively, when the present device is used, the glass needle 8 is installed into the needle holder 7, the filter cartridge gun head 1 is inserted and connected to the suction nozzle 2, and then the filter cartridge gun head 1 is held in the mouth, while the needle holder 7 is held in the right hand. During the egg detection and transplantation process, the fertilized egg is sucked into or removed from the glass needle 8 by utilizing the suction force of the mouth, thereby realizing the egg detection and transplantation operation. Compared with the prior art, the present device can solve the problem of cross infection caused by the oral cavity and the suction nozzle 2 through the filter cartridge gun head 1. At the same time, when the fertilized egg is inhaled, if the suction force is too high, causing part of the liquid to enter the rubber tube from the glass needle 8, the liquid can be absorbed by the cotton plug 6 to avoid the inhaled liquid from directly contacting the rubber tube, which can solve the hygiene problem well and has the advantage of high practicality.

[0042] In one embodiment, it is more preferred that a filter 4 is connected to the middle of the rubber tube, and the filter 4 divides the rubber tube into an upper rubber tube 3 and a lower rubber tube 5 .

[0043] It should be noted that the filter 4 is mainly used to filter the air in the rubber hose. During the filtering process, the flow rate of the gas can be slowed down to prevent the liquid from rising too quickly. At the same time, it is convenient for temporary placement and fixation on the eyepiece barrel of the microscope.

[0044] Among them, during the entire experimental operation, it is necessary to perform other steps intermittently. At this time, the entire device needs to be temporarily placed aside. At this time, the entire device can be hung on the stereo microscope being operated, and the setting of the filter 4 can place the rubber tube and flip it on the stereo microscope to facilitate the temporary placement of the device.

[0045] In one embodiment, the device is suitable for the egg detection process after in vitro fertilization of mice. During the egg detection process, it is necessary to quickly select fertilized eggs (about 100um in diameter) from the culture medium containing impurities and continuously transfer them to fresh egg washing solution for 2 to 3 times to remove impurities attached to the embryo. When the diameter of the egg detection needle is small, the fertilized eggs selected are relatively clean, but the number of eggs stored is limited. When the diameter is large, it is easy to absorb many impurities.

[0046] Reference Figure 2 as well as Figure 3 As shown, in this embodiment, the glass needle 8 is further refined as a whole, and the glass needle 8 is used as an egg detection needle as a whole. The glass needle 8 includes a holding section 9 and a capillary section 10. The holding section 9 is used for installation and connection with the needle body holder 7. One end of the capillary section 10 is connected to the holding section 9, and the other end is a needle tip 11, and the needle tip 11 is set in a conical transition.

[0047] Through the arrangement of this structure, the glass needle 8 as a whole can not only have a smaller needle structure, but its subsequent tapered capillary section 10 that gradually expands can also store the sucked fertilized eggs, and has high practicality.

[0048] In this embodiment, the needle mouth of the needle tip 11 is rounded and smooth. In addition, the inner diameter of the needle mouth can be selected to be 100-120um, and the inner diameter of the capillary segment 10 can be selected to be 200um-300um. Specifically, in this embodiment, the inner diameter of the needle mouth is specifically 110um, and the inner diameter of the capillary segment 10 is specifically 250um. In the egg detection process, by providing a needle tip 11 with a small front diameter, a large rear diameter, and a smooth transition in a cone, the front end of the needle tip 11 can avoid sucking in too much impurities in the liquid, and the capillary segment 10 at the rear end of the needle tip 11 can also ensure the storage capacity of fertilized eggs and improve the egg detection rate.

[0049] Reference Figure 4 as well as Figure 5As shown, in one embodiment, the device as a whole is suitable for embryonic fallopian tube transplantation in mice. In the embryonic fallopian tube transplantation, a large diameter of the transplantation needle will lead to a large wound in the fallopian tube, and it is difficult to enter the fallopian tube if it is too large. A small diameter is conducive to transplantation but easy to block, and a small diameter is easy to squeeze the embryo, making it difficult for the embryo and the culture medium to go down. In addition, the inappropriate size and angle of the transplantation needle inserted into the fallopian tube can also easily cause problems such as blockage or reflux.

[0050] In this embodiment, the glass needle 8 is further refined as a whole. The glass needle 8 is specifically used as a transplant needle. The glass needle 8 includes a holding section 9 and a capillary section 10, wherein the holding section 9 is used to be installed and connected with the needle body holder 7. One end of the capillary section 10 is connected to the holding section 9, and the other end is a needle tip 11. The needle tip 11 and the capillary section 10 are arranged in a deflected manner, and the deflection angle can be selected to be 15-30 degrees, and the deflection length of the needle tip 11 can be selected to be 0.5-1mm. Specifically, in this embodiment, the deflection angle is set to 20 degrees, and the deflection length of the needle tip 11 is 1mm.

[0051] It should be noted that the deflection setting of the needle tip 11 can make the needle tip 11 of the glass needle 8 parallel to the fallopian tube after entering the fallopian tube, which is beneficial to the subsequent transplantation operation. At the same time, the deflection length setting of the needle tip 11 can also facilitate the control of the depth of the glass needle 8 entering the fallopian tube, avoid needle blockage or reflux by the fallopian tube wall, and help improve the success rate of transplantation.

[0052] The inner diameter of the capillary segment 10 can be selected to be 100-120 um. In this embodiment, specifically, the inner diameter of the capillary segment 10 is 120 um. In addition, the needle opening of the needle tip 11 is rounded and smooth.

[0053] By providing a needle tip 11 slightly larger than the diameter of a 2-cell embryo (about 100 um), the problem of easy blockage, easy squeezing of the embryo, and difficulty in the downward movement of the embryo and the culture medium can be solved, which is conducive to improving the success rate of transplantation. In addition, by providing a smooth and round needle tip 11, it is conducive to avoiding damage to the fertilized egg, embryo, fallopian tube, etc. by the needle tip 11.

[0054] In one embodiment, the device is equipped with two glass needles 8, one is the above-mentioned egg detection needle, and the other is the above-mentioned transplantation needle;

[0055] The structure of the egg detection needle is as follows: the glass needle 8 includes a holding section 9 and a capillary section 10. The holding section 9 is used to be installed and connected with the needle body holder 7. One end of the capillary section 10 is connected to the holding section 9, and the other end is a needle tip 11. The needle tip 11 is set in a conical transition, and the needle mouth of the needle tip 11 is rounded and smooth. Specifically, in this embodiment, the inner diameter of the needle mouth is specifically 110um, and the inner diameter of the capillary section 10 is specifically 250um;

[0056] The structure of the transplant needle is as follows: the glass needle 8 includes a holding section 9 and a capillary section 10, wherein the holding section 9 is used to be installed and connected with the needle body holder 7. One end of the capillary section 10 is connected to the holding section 9, and the other end is a needle tip 11, and the needle tip 11 and the capillary section 10 are arranged in a deflected manner. In this embodiment, the deflection angle is set to 20 degrees, and the deflection length of the needle tip 11 is 1 mm, and the inner diameter of the capillary section 10 is 120 um. In addition, the needle tip 11 has a rounded and smooth needle opening. Specific implementation method:

[0058] When testing eggs, the egg testing needle is selected as a glass needle 8 and installed in the needle holder 7. The filter cartridge gun head 1 is held in the mouth while the needle holder 7 is held in the right hand. The egg testing needle is inserted into the drop of in vitro fertilization culture medium under a stereo microscope. The needle tip 11 is close to the fertilized egg and the fertilized egg is sucked into the glass needle 8 by the suction of the mouth. As little liquid and impurities as possible are sucked. All fertilized eggs in the culture medium are continuously sucked and transferred to new culture medium. This is repeated for a total of 3 times to complete the washing of the fertilized eggs (i.e., egg testing).

[0059] During transplantation, the transplantation needle is selected as the glass needle 8 and installed in the needle holder 7. The filter cartridge gun head 1 is held in the mouth while the needle holder 7 is held in the right hand. Under a stereomicroscope, the culture fluid and air are alternately sucked twice at intervals of 2-3 mm to make 2-3 small bubbles. Then, the 2-cell embryos are sucked into the glass needle 8 one by one in the culture fluid using oral suction until the last embryo is located above the deflection. The intervals between embryos are as small as possible. The transplantation needle is inserted into the fallopian tube through the opening on the fallopian tube at the front end of the mouse enlargement along the direction from the ovary to the uterus. When the deflection contacts the fallopian tube, the needle tip 11 of the transplantation needle is stopped, and the fallopian tube is parallel to the fallopian tube. The mouth is gently blown to inject the embryo in the transplantation needle into the fallopian tube. After observing that small bubbles are generated in the enlargement, the transplantation needle can be withdrawn.

[0060] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the utility model.

Claims

1. An egg detection and transplantation device, characterized in that: include: Rubber hose; A suction nozzle connected to one end of the rubber tube; A filter cartridge gun head, the filter cartridge gun head being connected to the suction nozzle; A needle holder, the needle holder being connected to the other end of the rubber tube; a glass needle, wherein the glass needle is disposed on the needle holder; and A cotton plug is arranged inside the rubber tube and contacts the end of the glass needle.

2. The egg detection and transplantation device according to claim 1, characterized in that: The middle part of the rubber tube is connected with a filter.

3. The egg detection and transplantation device according to claim 1, characterized in that: The glass needle comprises: a holding section, the holding section being used for being mounted and connected with the needle holder; and A capillary segment, one end of which is connected to the holding segment, and the other end of which is a needle tip, and the needle tip is arranged in a conical transition.

4. The egg detection and transplantation device according to claim 3, characterized in that: The needle mouth of the needle tip is round and smooth.

5. The egg detection and transplantation device according to claim 4, characterized in that: The inner diameter of the needle opening is 100-120um, and the inner diameter of the capillary section is 200um-300um.

6. The egg detection and transplantation device according to claim 1, characterized in that: The glass needle comprises: a holding section, the holding section being used for being mounted and connected with the needle holder; and A capillary segment, one end of which is connected to the holding segment, and the other end of which is a needle tip, wherein the needle tip and the capillary segment are arranged in a deflected manner, and the deflection angle is 15-30 degrees.

7. The egg detection and transplantation device according to claim 6, characterized in that: The inner diameter of the capillary section is 100-120um.

8. The egg detection and transplantation device according to claim 6, characterized in that: The deflection length of the needle tip is 1 mm.

9. The egg detection and transplantation device according to claim 6, characterized in that: The needle mouth of the needle tip is round and smooth.

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