Mixer for preparing cell culture medium

Abstract

The utility model discloses a mixer for preparing a cell culture medium, which comprises a dissolving barrel and a mixing barrel, a fixed pipe and a hopper are arranged on the dissolving barrel, a rotating pipe is rotatably arranged at the bottom of the fixed pipe, a charging barrel is arranged on the rotating pipe, a material hole is formed in the charging barrel, a rotating device is arranged on the dissolving barrel, a plug is arranged on the dissolving barrel, and a stirring frame is arranged on the mixing barrel. A driving device is arranged on the stirring frame, a stirring pipe is rotationally arranged on the stirring frame, stirring blades are arranged on the stirring pipe, a circulating pipe and a water outlet pipe are arranged on the stirring pipe, a circulating pump is arranged in the circulating pipe, a circulating disc is arranged at the bottom of the stirring pipe in a communicating mode, and circulating holes are formed in the circulating disc. The material barrel is driven to rotate through the rotating device, the stirring pipe and the stirring blades are driven to rotate through the driving device, nutrient substances in the mixing barrel are conveniently stirred, the mixing efficiency is improved, the nutrient substances are driven to circulate through the circulating pump, and the mixing effect and the mixing efficiency are further improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, and more specifically, to a mixer for preparing cell culture medium. Background Art

[0002] Cell culture medium is a culture medium that provides appropriate nutrients and environmental conditions to support the growth, proliferation and maintenance of functions of cells in vitro. The formula and composition of cell culture medium usually vary according to the specific cell type, culture conditions and application requirements. Correct selection and configuration of culture medium is one of the keys to in vitro cell culture, which can affect the growth status, health status of cells and the reliability of experimental results.

[0003] When dissolving powdered raw materials, although the powdered raw materials can be dissolved in water, due to the small particle size of the powdered materials during the dissolution preparation, agglomerates are easily formed in the initial stage of dissolution and are difficult to dissolve in water, which affects the initial dissolution rate of the raw materials. Traditionally, the mixing of raw materials for cell culture is generally done by manual mixing, which increases the labor intensity of operators and causes low production efficiency. At the same time, the raw materials cannot be evenly stirred and mixed, resulting in poor mixing effect of the culture medium raw materials. Utility Model Content

[0004] In view of the problems existing in the prior art, the utility model provides a mixer for preparing a cell culture medium to solve the technical problems of slow dissolution efficiency and poor mixing effect mentioned in the background technology.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a mixer for preparing cell culture medium, comprising a dissolving barrel and a mixing barrel, the dissolving barrel is provided with a fixed tube, the top of the fixed tube is provided with a hopper, the bottom of the fixed tube is rotatably provided with a rotating tube, the rotating tube is provided with a barrel, the barrel is provided with a material hole, the dissolving barrel is provided with a rotating device, the dissolving barrel is provided with a plug, the mixing barrel is provided with a stirring frame, the stirring frame is provided with a driving device, the stirring frame is rotatably provided with a stirring tube, the stirring tube is provided with stirring blades, the stirring tube is provided with a circulation tube and a water outlet pipe, the circulation tube is provided with a circulation pump, the water inlet end of the circulation pump is connected with the stirring tube, the water outlet end is connected with the water outlet pipe, the bottom of the stirring tube is connected with a circulation disk, and the circulation disk is provided with a circulation hole.

[0006] The utility model is further configured that the dissolving barrel is provided with a water adding barrel and a water adding pipe, so as to facilitate adding ultrapure water into the dissolving barrel.

[0007] The utility model is further configured that the rotating device includes a rotating motor, a rotating shaft is provided on the motor shaft of the rotating motor, and rotating gears are provided on the rotating shaft and the rotating tube to drive the barrel to rotate.

[0008] The utility model is further configured that the driving device includes a driving tube, a conductive slider is arranged in the driving tube, and the conductive slider is connected to the circulation pump through an electric wire to facilitate power supply to the circulation pump.

[0009] The utility model is further configured that the stirring frame is provided with a stirring motor, the motor shaft of the stirring motor is provided with a first gear, the driving tube is provided with a second gear, and the stirring tube is driven to rotate by the stirring motor.

[0010] The utility model is further configured that a connecting block is provided on the stirring blade, connecting plates are provided on both sides of the connecting block, and limiting plates are provided on adjacent connecting plates, so as to facilitate limiting the positions of the connecting block and the connecting plates.

[0011] The utility model is further configured that the stirring tube is provided with a connecting protrusion matched with the connecting plate, and the stirring blade is driven to rotate through the stirring tube through the connecting protrusion.

[0012] The utility model is further configured such that limiting bolts are provided between the connecting protrusion, the connecting plate and the limiting plate, so as to fix the positions of the limiting plate and the connecting plate, and further fix the position of the stirring blade.

[0013] Compared with the prior art, the utility model provides a mixer for preparing cell culture medium, which has the following beneficial effects:

[0014] 1. Powder materials are added into the barrel through the hopper, and the barrel is driven to rotate by the rotating device, so that the powder materials rotate centrifugally in the barrel, and enter the dissolution barrel through the material hole to be mixed with ultrapure water for centrifugal dissolution, so that the powder materials are evenly dispersed in the ultrapure water, thereby improving the dissolution rate of the powder raw materials.

[0015] 2. The driving device drives the stirring tube and the stirring blades to rotate, which facilitates the stirring of the nutrients in the mixing barrel and improves the mixing efficiency. The circulating pump drives the nutrients to circulate, further improving the mixing effect and mixing efficiency.

[0016] 3. By providing the connecting protrusion, the connecting plate and the limiting plate, it is convenient to connect and fix the stirring rod, thereby improving the convenience of the mixer assembly. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the overall structure of a mixer for preparing a cell culture medium in the utility model;

[0018] Figure 2 This is a schematic diagram of the internal structure of the dissolving barrel in the utility model;

[0019] Figure 3This is a schematic diagram of the internal structure of the mixing barrel in the utility model;

[0020] Figure 4 It is a schematic diagram of the overall structure of the driving device in the utility model;

[0021] Figure 5 It is a structural schematic diagram of the stirring blade, the connecting block, the connecting plate, the limiting plate, the connecting protrusion and the limiting bolt in the utility model.

[0022] In the figure: 1. dissolving barrel; 2. mixing barrel; 3. fixed pipe; 4. hopper; 5. rotating pipe; 6. barrel; 7. material hole; 8. plug; 9. stirring frame; 10. stirring pipe; 11. stirring blade; 12. circulation pipe; 13. water outlet pipe; 14. circulation pump; 15. circulation disk; 16. circulation hole; 17. water adding barrel; 18. water adding pipe; 19. rotating motor; 20. rotating shaft; 21. rotating gear; 22. driving pipe; 23. conductive slider; 24. stirring motor; 25. first gear; 26. second gear; 27. connecting block; 28. connecting plate; 29. ​​limit plate; 30. connecting protrusion; 31. limit bolt. DETAILED DESCRIPTION

[0023] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present utility model will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0024] It should be noted that, unless otherwise specified, all technical and scientific terms used in this application have the same meanings as commonly understood by ordinary technicians in the technical field to which this application belongs.

[0025] In the present invention, unless otherwise specified, directions such as "up" and "down" are usually used in reference to the directions shown in the drawings, or in reference to the vertical, perpendicular or gravity direction; similarly, for ease of understanding and description, "left" and "right" are usually used in reference to the left and right shown in the drawings; "inside" and "outside" refer to the inside and outside relative to the outline of each component itself, but the above-mentioned directions are not used to limit the present invention.

[0026] See also Figure 1-5A mixer for preparing a cell culture medium comprises a dissolving barrel 1 and a mixing barrel 2. The dissolving barrel 1 is provided with a fixed tube 3. A hopper 4 is provided on the top of the fixed tube 3. A rotating tube 5 is rotatably provided on the bottom of the fixed tube 3. A barrel 6 is provided on the rotating tube 5. A material hole 7 is provided on the barrel 6. The dissolving barrel 1 is provided with a rotating device. A plug 8 is provided on the dissolving barrel 1. The mixing barrel 2 is provided with a stirring frame 9. A driving device is provided on the stirring frame 9. A stirring tube 10 is rotatably provided on the stirring frame 9. A stirring blade 11 is provided on the stirring tube 10. A circulation pipe 12 and a water outlet pipe 13 are provided on the stirring tube 10, a circulation pump 14 is provided in the circulation pipe 12, a water inlet end of the circulation pump 14 is connected to the stirring tube 10, and a water outlet end is connected to the water outlet pipe 13, a circulation disk 15 is provided at the bottom of the stirring tube 10, a circulation hole 16 is provided on the circulation disk 15, a water adding bucket 17 and a water adding pipe 18 are provided on the dissolving barrel 1, and the rotating device includes a rotating motor 19, a rotating shaft 20 is provided on the motor shaft of the rotating motor 19, and a rotating gear 21 is provided on the rotating shaft 20 and the rotating tube 5.

[0027] In this embodiment, when dissolving, ultrapure water is added to the dissolving barrel 1 through the water adding barrel 17 and the water adding pipe 18, and the powder material is added to the barrel 6 through the hopper 4, and the rotating motor 19 is started. The rotating motor 19 drives the rotating shaft 20 to rotate, and the rotating shaft 20 drives the rotating tube 5 to rotate through the rotating gear 21. The rotating tube 5 drives the barrel 6 to rotate, and the material hole 7 set inside the barrel 6 forms a mixed centrifugal dissolution with ultrapure water, which can make the powder raw material more evenly dispersed inside the ultrapure water, which is used to increase the dissolution rate of the initial powder raw material, and then the plug 8 is manually opened to reverse the dissolved nutrients.

[0028] See also Figure 3-5 As an implementation method of the mixing barrel 2: the driving device includes a driving tube 22, a conductive slider 23 is provided in the driving tube 22, the conductive slider 23 is connected to the circulation pump 14 through an electric wire, a stirring motor 24 is provided on the stirring frame 9, a first gear 25 is provided on the motor shaft of the stirring motor 24, a second gear 26 is provided on the driving tube 22, a connecting block 27 is provided on the stirring blade 11, connecting plates 28 are provided on both sides of the connecting block 27, and limit plates 29 are provided on adjacent connecting plates 28, a connecting protrusion 30 matching the connecting plate 28 is provided on the stirring tube 10, and a limit bolt 31 is provided between the connecting protrusion 30, the connecting plate 28 and the limit plate 29.

[0029] More specifically, when installing the stirring blade 11, the connecting plate 28 of the stirring blade 11 is engaged with the connecting protrusion 30, and the limiting plate 29 is laid on the connecting plate 28 to fix the position of the connecting plate 28 and the stirring blade 11. The limiting bolt 31 connects and fixes the limiting plate 29, the connecting plate 28 and the connecting protrusion 30, thereby fixing the stirring blade 11. The dissolved nutrients are added to the mixing barrel 2, the conductive slider 23 is connected to the external power supply, the stirring motor 24 and the circulation pump 14 are started, the stirring motor 24 drives the first gear 25 to rotate, the first gear 25 drives the second gear 26 and the driving tube 22 to rotate, the driving tube 22 drives the stirring tube 10 and the stirring blade 11 to rotate, the nutrient solution is stirred and mixed by the stirring blade 11, the circulation pump 14 absorbs the bottom solution through the circulation disk 15 and the circulation hole 16, and discharges it to the top of the liquid surface through the outlet pipe 13, so as to facilitate the circulation of the bottom and top solutions in the mixing barrel 2, thereby improving the stirring and mixing efficiency.

[0030] In summary, when the overall equipment is in use or running: when dissolving, ultrapure water is added to the dissolving barrel 1 through the water adding barrel 17 and the water adding pipe 18, and the powder material is added to the barrel 6 through the hopper 4, and the rotating motor 19 is started. The rotating motor 19 drives the rotating shaft 20 to rotate, and the rotating shaft 20 drives the rotating tube 5 to rotate through the rotating gear 21, and the rotating tube 5 drives the barrel 6 to rotate. The material hole 7 set inside the barrel 6 forms a mixed centrifugal dissolution with ultrapure water, which can make the powder raw material more evenly dispersed inside the ultrapure water, which is used to increase the dissolution rate of the initial powder raw material, and then the plug 8 is manually opened to reverse the dissolved nutrients.

[0031] When installing the stirring blade 11, the connecting plate 28 of the stirring blade 11 is engaged with the connecting protrusion 30, and the limiting plate 29 is laid on the connecting plate 28 to fix the position of the connecting plate 28 and the stirring blade 11. The limiting bolt 31 connects and fixes the limiting plate 29, the connecting plate 28 and the connecting protrusion 30, and then fixes the stirring blade 11. The dissolved nutrients are added to the mixing barrel 2, the conductive slider 23 is connected to the external power supply, the stirring motor 24 and the circulation pump 14 are started, the stirring motor 24 drives the first gear 25 to rotate, the first gear 25 drives the second gear 26 and the driving tube 22 to rotate, the driving tube 22 drives the stirring tube 10 and the stirring blade 11 to rotate, the nutrient solution is stirred and mixed by the stirring blade 11, the circulation pump 14 absorbs the bottom solution through the circulation disk 15 and the circulation hole 16, and discharges it to the top of the liquid surface through the outlet pipe 13, so as to facilitate the circulation of the bottom and top solutions in the mixing barrel 2, thereby improving the stirring and mixing efficiency.

[0032] In all the schemes mentioned above, the connection between two parts can be selected according to actual conditions by welding, bolt and nut matching connection, bolt or screw connection or other well-known connection methods, which are not described here one by one. In the above, all fixed connections are preferably welded. Although the embodiments of the utility model have been shown and described, it can be understood by ordinary technicians in this field that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the utility model. The scope of the utility model is defined by the attached claims and their equivalents.

Claims

1. A mixer for preparing a cell culture medium, comprising a dissolution tank (1) and a mixing tank (2), characterized in that: The dissolving barrel (1) is provided with a fixed tube (3), the top of the fixed tube (3) is provided with a hopper (4), the bottom of the fixed tube (3) is rotatably provided with a rotating tube (5), the rotating tube (5) is provided with a barrel (6), the barrel (6) is provided with a material hole (7), the dissolving barrel (1) is provided with a rotating device, the dissolving barrel (1) is provided with a plug (8), the mixing barrel (2) is provided with a stirring frame (9), the stirring frame (9) is provided with a driving device, the stirring frame ( A stirring tube (10) is rotatably provided on the stirring tube (9), a stirring blade (11) is provided on the stirring tube (10), a circulation tube (12) and a water outlet tube (13) are provided on the stirring tube (10), a circulation pump (14) is provided in the circulation tube (12), a water inlet end of the circulation pump (14) is connected to the stirring tube (10), and a water outlet end is connected to the water outlet tube (13), a circulation disk (15) is provided at the bottom of the stirring tube (10), and a circulation hole (16) is provided on the circulation disk (15).

2. A mixer for preparing cell culture medium according to claim 1, characterized in that: The dissolving barrel (1) is provided with a water adding barrel (17) and a water adding pipe (18).

3. The mixer for preparing cell culture medium according to claim 1, characterized in that: The rotating device comprises a rotating motor (19), a rotating shaft (20) is provided on the motor shaft of the rotating motor (19), and a rotating gear (21) is provided on the rotating shaft (20) and the rotating tube (5).

4. The mixer for preparing cell culture medium according to claim 1, characterized in that: The driving device comprises a driving tube (22), a conductive slider (23) is arranged in the driving tube (22), and the conductive slider (23) is connected to the circulation pump (14) via an electric wire.

5. A mixer for preparing a cell culture medium according to claim 4, characterized in that: The stirring frame (9) is provided with a stirring motor (24), a motor shaft of the stirring motor (24) is provided with a first gear (25), and the driving tube (22) is provided with a second gear (26).

6. The mixer for preparing cell culture medium according to claim 1, characterized in that: A connecting block (27) is provided on the stirring blade (11), connecting plates (28) are provided on both sides of the connecting block (27), and limiting plates (29) are provided adjacent to the connecting plates (28).

7. A mixer for preparing a cell culture medium according to claim 6, characterized in that: The stirring tube (10) is provided with a connecting protrusion (30) that cooperates with the connecting plate (28).

8. A mixer for preparing a cell culture medium according to claim 7, characterized in that: A limiting bolt (31) is provided between the connecting protrusion (30), the connecting plate (28) and the limiting plate (29).

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