Simple stratified sampling centrifugal tube

Abstract

The utility model relates to the technical field of medical detection appliances, and particularly discloses a simple stratified sampling centrifuge tube which comprises a tube body and a tube cover, the tube cover is detachably buckled at the top of the tube body in a sealing manner, and a bendable connecting strip is connected between the tube body and the tube cover; a sampling structure is arranged at the position, close to the bottom, of the tube body and comprises a sampling hole formed in the tube body and a sampling tube arranged in the tube body, a sealing plug is detachably connected into the sampling hole, one end of the sampling tube is communicated with the sampling hole, a sealing film is arranged at the other end of the sampling tube, and the sealing film is elastic. A sampling notch is formed in the sealing film. By adopting the technical scheme provided by the utility model, the technical problem that the accuracy of a detection result is influenced due to the fact that the sucked lower-layer sample is easy to be impure when the lower-layer sample is taken and used by the centrifugal tube in the prior art can be solved.

Description

Technical Field

[0001] The utility model relates to the technical field of medical detection tools, in particular to a simple layered sampling centrifuge tube. Background Art

[0002] Centrifuge tubes are small containers commonly used in laboratories, specially designed for sample separation in centrifuges. Figure 1 As shown, the prior art centrifuge tube includes a tube body 10 and a tube cover 20. The top of the tube body 10 is opened to form a tube mouth, and the bottom of the tube body 10 is a conical structure; the tube cover 20 is a press-type sealing cover, and the tube cover 20 is connected to one side of the tube mouth through a bendable connecting strip. When in use, after adding the sample to the centrifuge tube, the tube mouth is sealed by the tube cover, and then the centrifuge tube is placed in a centrifuge for centrifugal treatment, so that the sample is layered in the centrifuge tube, and then the centrifuge tube is taken out of the centrifuge, the tube cover is opened, and the pipette tip is inserted into the centrifuge tube through the tube mouth, and the sample corresponding to the layer in the centrifuge tube is taken for use.

[0003] In some medical testing and experimental operations, it is necessary to take the lower layer sample after centrifugation for use, and it is very important to maintain the purity of the lower layer sample when sampling. For example, when conducting medical tests such as anemia type identification, red blood cell count, and hemoglobin content measurement on patients, whole blood is collected for centrifugation. After centrifugation, the whole blood is roughly divided into three layers, from top to bottom, namely the plasma layer (serum layer), the white blood cell layer, and the red blood cell layer. Taking the lower layer red blood cell sample for use and maintaining the purity of the lower layer red blood cell sample when sampling can ensure the accuracy of the red blood cell test results. For another example, when conducting biochemical tests, drug concentration determination, viral marker detection, hormone level analysis, electrolyte assessment and other medical tests on patients with hyperlipidemia, whole blood is collected for centrifugation. The upper serum sample contains a large amount of lipid substances and is turbid milky white, which is called a lipemia sample. The lipemia sample needs to be centrifuged a second time. After the second centrifugation, the lipemia sample is roughly divided into two layers, from top to bottom, the chylomicron blood lipid layer and the transparent and clear serum layer. The lower serum sample is taken for use. Maintaining the purity of the lower serum sample during sampling can ensure the accuracy of the serum test data.

[0004] The use of the prior art centrifuge tube for the above-mentioned medical testing operation of removing the lower layer sample will have the following problems: the pipette tip extends downward into the centrifuge tube through the tube mouth, and the pipette tip needs to pass through the upper layer sample before it can absorb the lower layer sample; on the one hand, when the pipette tip passes through the upper layer sample to enter the lower layer sample, it is easy to stir the junction between the upper layer sample and the lower layer sample, so that the upper layer sample is mixed into the lower layer sample, resulting in impure lower layer sample; on the other hand, when the pipette tip passes through the upper layer sample, part of the upper layer sample is easy to enter the pipette tip first or adhere to the surface of the tip, which also results in impure lower layer sample; therefore, it is difficult to ensure the accuracy of subsequent test results. Utility Model Content

[0005] The utility model aims to provide a simple stratified sampling centrifuge tube to solve the technical problem that when the centrifuge tube in the prior art takes out the lower layer sample for use, the lower layer sample is easily sucked impure, thereby affecting the accuracy of the detection result.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] A simple layered sampling centrifuge tube comprises a tube body and a tube cover, wherein the tube cover is detachably sealed and buckled on the top of the tube body, and a bendable connecting strip is connected between the tube body and the tube cover; a sampling structure is provided on the tube body near the bottom, and the sampling structure comprises a sampling hole opened on the tube body and a sampling tube arranged in the tube body, a sealing plug is detachably connected in the sampling hole, one end of the sampling tube is connected to the sampling hole, and a sealing film is provided on the other end of the sampling tube.

[0008] The principle and beneficial effects of this program:

[0009] In actual application, open the tube cover and add the sample into the tube body. Since the sealing plug seals the sampling hole, that is, the sealing plug seals one end of the sampling tube and the sealing film seals the other end of the sampling tube, the sample in the tube body will not enter the sampling tube, and will not flow out of the tube body through the sampling tube and the sampling hole. Close the tube cover, put the centrifuge tube into the centrifuge, and centrifuge the sample so that the sample is layered in the centrifuge tube. After the centrifugation is completed, take the centrifuge tube out of the centrifuge, then take the sealing plug out of the sampling hole, and use a pipette to take the lower layer sample in the centrifuge tube through the sampling structure. Specifically, the pipette tip is inserted into the sampling tube through the sampling hole, and the pipette is forced so that the pipette tip pierces the sealing film and extends into the position near the bottom of the tube body, quantitatively absorbs the lower layer sample, and then the pipette tip is taken out from the sampling tube and the sampling hole, and the sampling hole is re-sealed with a sealing plug to prevent the remaining sample in the tube body from flowing out.

[0010] 1. In this scheme, the sampling structure is arranged near the bottom of the tube body. After the centrifugation is completed, the pipette tip can be directly extended into the tube body near the bottom through the sampling structure, so that the pipette tip can directly absorb the lower sample without passing through the upper sample. This can prevent the pipette tip from muddying the junction between the upper and lower samples when passing through the upper sample to enter the lower sample, thereby causing the lower sample to be mixed with the upper sample, and can also prevent part of the upper sample from entering the pipette tip first when passing through the upper sample, thereby ensuring the purity of the lower sample absorbed by the pipette tip, and further ensuring the accuracy of subsequent test results.

[0011] 2. In this scheme, a sampling tube is arranged at the sampling hole to be connected to the tube body, and a sealing film is arranged at the end of the sampling tube away from the sampling hole. In the centrifugation process after sample addition, the sealing film and the sealing plug play a double sealing role on the sampling structure, thereby improving the sealing of the tube body and preventing the sample in the tube body from flowing out of the tube body from the sampling structure. In addition, the sealing plug seals the sampling hole to keep the sampling tube clean. In the sampling operation after the centrifugation is completed, even if the pipette tip contacts the sampling tube, the pipette tip can be prevented from being contaminated, thereby ensuring the purity of the lower layer sample sucked.

[0012] 3. The sealing film and sampling tube of the present scheme form a buffer space between the sealing plug and the inside of the tube body. When the sampling operation is performed after the sample is centrifuged, removing the sealing plug from the sampling hole will not cause the sample in the tube body to flow out directly from the sampling hole, thereby ensuring the smooth progress of the sampling operation, which can not only avoid sample waste, but also avoid two-way contamination of the sample and the experimental environment; and when the pipette tip is taken out after the sampling is completed, even if the sample in the tube body will flow out from the position where the sealing film is punctured, it will first flow into the sampling tube, and will not flow out of the tube body through the sampling hole until the sampling tube is filled, thus reserving time for the operator to re-seal the sampling hole with the sealing plug, further preventing the sample in the tube body from flowing out.

[0013] Preferably, as an improvement, the sealing film is elastic and a sampling incision is provided on the sealing film.

[0014] Beneficial effect: The sealing film of the present invention is elastic. When a sampling incision is opened on the sealing film, the sampling incision will be closed under the elastic squeezing action of the sealing film to ensure the airtightness of the sealing film. On the one hand, the sampling structure can still be double-sealed with the sealing plug. On the other hand, a buffer space can still be formed between the sealing plug and the inside of the tube body to isolate the sample outside the sampling tube and prevent the sample from flowing out of the tube body from the sampling hole when the sealing plug is opened.

[0015] When the sample is centrifuged and the sampling operation is performed, the pipette tip is inserted into the sampling tube through the sampling hole. When the pipette is forced, the pipette tip squeezes the sampling incision open and extends into the tube body near the bottom through the sampling incision to complete the sampling of the lower layer sample. And when the sampling is completed and the pipette tip is withdrawn, the sampling incision will be re-sealed under the elastic squeezing action of the sealing film, and the sample will continue to be isolated outside the sampling tube to prevent the lower layer sample or even the upper layer sample from flowing into the sampling tube. Not only can secondary sampling be performed later, but the sampling hole can also be re-sealed with a sealing plug at any time. There is no need to worry about the sample filling the sampling tube and flowing out of the tube body through the sampling hole, which improves practicality.

[0016] Preferably, as an improvement, the sampling tube is arranged obliquely, and the sealing film is arranged horizontally.

[0017] Beneficial effect: This scheme sets the sampling tube at an angle, which can achieve the horizontal setting of the sealing film. Setting the sealing film horizontally can avoid the centrifugal force during the centrifugation of the sample, and prevent the sampling incision from being squeezed open under the action of the centrifugal force, thereby preventing the sample from entering the sampling tube through the sampling incision during the centrifugation process, causing the sampling tube and even the sampling hole and the sealing plug to be contaminated by the sample that has not been centrifuged, ensuring that the pure lower layer sample can be smoothly obtained through the sampling structure after the centrifugation is completed.

[0018] Preferably, as an improvement, the sampling hole is arranged obliquely, and the axis of the sampling hole coincides with the axis of the sampling tube.

[0019] Beneficial effects: In this scheme, the sampling hole and the sampling tube are coaxially tilted. On the one hand, the sealing plug can be inserted into the sampling tube through the sampling hole, increasing the contact area of ​​the sealing plug, thereby improving the connection stability and sealing effect of the sealing plug; on the other hand, the angle between the sampling hole and the sampling tube is eliminated, so the diameters of the sampling hole and the sampling tube can be set to match the diameter of the pipette tip, which is beneficial to reducing the volume of the sampling structure and ensuring that the pipette tip can smoothly pass through the sampling hole into the sampling tube, thereby improving the convenience of the sampling operation.

[0020] Preferably, as an improvement, the sealing plug comprises an elastic sealing portion, which is tightened in the sampling hole to achieve a detachable connection between the sealing plug and the sampling hole.

[0021] Beneficial effect: In this scheme, the sealing plug is configured to include an elastic sealing part, and the elastic sealing part is tightened in the sampling hole, which can effectively realize the detachable connection between the sealing plug and the sampling hole. It has a simple structure and is easy to operate. It can also effectively realize the sealing of the sampling hole, which can prevent the sample from flowing out of the tube body from the sampling hole and maintain a clean environment in the sampling tube, thereby ensuring the purity of the lower layer sample absorbed and the accuracy of subsequent test results.

[0022] Preferably, as an improvement, the sealing plug further comprises a hard limiting portion arranged outside the elastic sealing portion, and the size of the hard limiting portion is larger than the size of the elastic sealing portion.

[0023] The outer side of the elastic sealing part refers to the side of the elastic sealing part away from the sampling tube.

[0024] Beneficial effects: In this scheme, a hard limiting part is arranged on the outside of the elastic sealing part, and the size of the hard limiting part is larger than that of the elastic sealing part. On the one hand, when the elastic sealing part is inserted into the sampling hole and tightened, the hard limiting part can limit the elastic sealing part to avoid the elastic limiting part being over-inserted and difficult to remove; on the other hand, when the elastic limiting part is taken out of the sampling hole, it can be operated through the hard limiting part located outside the sampling hole, thereby improving the convenience of removal.

[0025] Preferably, as an improvement, the hard limiting portion is arc-shaped, and the hard limiting portion can fit with the tube body.

[0026] Beneficial effect: This solution sets the hard limit part to an arc shape and fits the hard limit part with the tube body, which can effectively reduce the volume of the sampling structure exposed outside the tube body, thereby effectively reducing the radial volume of the entire centrifuge tube and ensuring that the centrifuge tube can be placed in a centrifuge for centrifugal treatment.

[0027] Preferably, as an improvement, a connecting rope is provided between the sealing plug and the connecting strip, and the length of the connecting rope is greater than the distance between the sealing plug and the connecting strip.

[0028] Beneficial effect: This solution connects the sealing plug to the connecting strip via a connecting rope, thereby preventing the sealing plug from being lost after being taken out of the sampling hole, thereby ensuring that the sealing plug can be used to re-seal the sampling hole later; and the length of the connecting rope is set to be greater than the distance between the sealing plug and the connecting strip, that is, when the sealing plug is connected to the sampling hole, the connecting rope has a surplus and is in a non-tensioned state, so that the operator can hold it. When the sealing plug needs to be taken out of the sampling hole, the sealing plug can be taken out of the sampling hole by pulling the connecting rope, thereby improving the convenience of removing the sealing plug. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a structural schematic diagram of a centrifuge tube in the prior art.

[0030] Figure 2 This is a schematic diagram of the internal structure of Example 1 of the utility model (the sealing plug is not inserted into the sampling hole).

[0031] Figure 3 for Figure 2 Schematic diagram of the structure at A in the middle.

[0032] Figure 4It is a schematic diagram of the top view of the sealing film in Example 1 of the utility model.

[0033] Figure 5 This is a schematic diagram of the structure of Example 2 of the utility model (the sealing plug is inserted into the sampling hole). DETAILED DESCRIPTION

[0034] The following is further described in detail through specific implementation methods:

[0035] The figure marks in the drawings of the specification include: tube body 10, cylindrical section 11, conical section 12, tube cover 20, connecting strip 21, sampling structure 30, sampling hole 31, sampling tube 32, sealing plug 40, elastic sealing part 41, hard limiting part 42, sealing membrane 50, sampling incision 51, connecting rope 60.

[0036] Example 1

[0037] Simple stratified sampling centrifuge tube, as attached Figure 2 As shown, it includes a tube body and a tube cover 20, and the tube cover 20 is tightened and buckled on the top of the tube body to achieve a detachable connection between the tube cover 20 and the tube body. A bendable connecting strip 21 is connected between the tube body and the tube cover 20, one end of the connecting strip 21 is fixed to the tube body, and the other end of the connecting strip 21 is fixed to the tube cover 20. The tube body includes a cylindrical section 11 and a conical section 12 integrally formed from top to bottom, and a sampling structure 30 is provided near the bottom of the tube body, specifically, the cylindrical section 11 of the tube body is provided with a sampling structure 30 near the conical section 12.

[0038] The sampling structure 30 includes a sampling hole 31 and a sampling tube 32. The sampling hole 31 is obliquely opened on the cylindrical section 11 and penetrates the cylindrical section 11. The sampling tube 32 is obliquely fixed on the inner wall of the tube body. The top of the sampling tube 32 is connected to the sampling hole 31, and the axis of the sampling tube 32 coincides with the axis of the sampling hole 31. In this embodiment, the side wall of the sampling tube 32 is fitted and fixed to the inner wall of the tapered section 12 of the tube body to increase the connection area between the sampling tube 32 and the tube body, improve the connection stability between the sampling tube 32 and the tube body, and prevent the sampling tube 32 from falling off during the centrifugation process.

[0039] A sealing plug 40 is detachably connected in the sampling hole 31. The sealing plug 40 includes an elastic sealing portion 41 and a hard limiting portion 42. The elastic sealing portion 41 is tightened in the sampling hole 31 to realize the detachable connection between the sealing plug 40 and the sampling hole 31; the hard limiting portion 42 is fixedly connected to the outside of the elastic sealing portion 41, and the size of the hard limiting portion 42 is larger than the size of the elastic sealing portion 41. The elastic sealing portion 41 is arranged at an angle, and after the elastic sealing portion 41 is inserted into the sampling hole 31 and tightened and fixed, the axis of the elastic sealing portion 41 coincides with the axis of the sampling hole 31. The hard limiting portion 42 is arc-shaped, so that the hard limiting portion 42 can fit with the tube body, reduce the radial size of the entire centrifuge tube, and ensure that the centrifuge tube can be placed in the centrifuge.

[0040] Combination Figure 3 and Figure 4 As shown, a sealing film 50 is horizontally arranged at the bottom end of the sampling tube 32, and a sampling incision 51 is formed on the sealing film 50, and the sealing film 50 is elastic, and the sampling incision 51 is closed under the elastic squeezing action of the sealing film 50. In this embodiment, the elastic sealing portion 41 of the sealing plug 40 and the sealing film 50 are both made of existing elastic materials such as rubber and silicone, and this embodiment is not specifically limited as long as the above functions can be achieved.

[0041] The specific implementation process is as follows:

[0042] (1) Sample addition: Add the sample to be centrifuged into the space outside the sampling tube 32 in the tube body, and tighten the tube cover 20 to fit on the top of the tube body to seal the top of the tube body. The elastic sealing portion 41 of the sealing plug 40 is tightened and inserted into the sampling hole 31 to seal the sampling hole 31, thereby sealing the top of the sampling tube 32. Since the sealing film 50 is elastic, the sampling incision 51 is closed under the elastic squeezing action of the sealing film 50, thereby sealing the bottom of the sampling tube 32. In this way, the sample added to the tube body will not enter the sampling tube 32, and will not flow out of the tube body through the sampling tube 32 and the sampling hole 31.

[0043] (2) Centrifugation: Place the centrifuge tube containing the sample into a centrifuge and centrifuge the sample to separate into layers in the centrifuge tube. Since the sealing film 50 is in a horizontal state, the sampling incision 51 can be prevented from being squeezed open by the horizontal outward centrifugal force, thereby preventing the uncentrifuged sample from entering the sampling tube 32 through the sampling incision 51 and contaminating the sampling tube 32 or even the sampling hole 31 and the sealing plug 40.

[0044] (3) Sampling: After the centrifugal stratification is completed, when it is necessary to take the lower layer sample for testing, remove the sealing plug 40 from the sampling hole 31, then tilt the pipette tip through the sampling hole 31 and insert it into the sampling tube 32, and tilt the pipette downward to force the pipette tip to squeeze the sampling incision 51 and insert it into the lower part of the tube body through the sampling incision 51, so that the lower layer sample located at the lower part of the tube body after centrifugation can be sampled smoothly. After sampling, the pipette tip is withdrawn from the sampling tube 32 and the sampling hole 31, and the sampling incision 51 is re-sealed under the elastic squeezing action of the sealing film 50, and then the sealing plug 40 is used to re-seal the sampling hole 31.

[0045] Since the lower end of the sampling tube 32 is located at the lower part of the tube body, the sampling incision 51 on the sealing film 50 is located at the lower part of the tube body; after the sample is stratified by centrifugation, the lower layer sample to be taken is also located at the lower part of the tube body, and the pipette tip can directly take the lower layer sample through the sampling incision 51 on the sealing film 50 without direct contact with the upper layer sample, thereby effectively avoiding the lower layer sample being contaminated by the previous sample, ensuring the purity of the lower layer sample, and then ensuring the accuracy of the test result.

[0046] Example 2

[0047] Simple stratified sampling centrifuge tube, as attached Figure 5 As shown, the difference between it and Example 1 is that: the sampling structure 30 is arranged just below the connecting strip 21, and a connecting rope 60 is arranged between the sealing plug 40 and the connecting strip 21, and the length of the connecting rope 60 is greater than the distance between the sealing plug 40 and the connecting strip 21. Specifically, one end of the connecting strip 21 is fastened and fixed to the connecting strip 21, and the other end of the connecting strip 21 is bonded and fixed to the middle position of the hard stopper 42 of the sealing plug 40. When the sealing plug 40 is connected to the sampling hole 31, the connecting rope 60 has a margin and is in a relaxed state, so that the operator can hold the connecting rope 60 to pull the sealing plug 40 out of the sampling hole 31 to realize the disassembly of the sealing plug 40.

[0048] The above is only an embodiment of the utility model, and the common knowledge such as the known specific technical solutions and / or characteristics in the solution is not described in detail here. It should be pointed out that for those skilled in the art, without departing from the technical solution of the utility model, several deformations and improvements can be made, which should also be regarded as the protection scope of the utility model, and these will not affect the effect of the implementation of the utility model and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A simple stratified sampling centrifuge tube, comprising a tube body and a tube cover, wherein the tube cover is detachably sealed and buckled on the top of the tube body, and a bendable connecting strip is connected between the tube body and the tube cover; the characteristics are: A sampling structure is provided near the bottom of the tube body, and the sampling structure includes a sampling hole opened on the tube body and a sampling tube arranged in the tube body. A sealing plug is detachably connected in the sampling hole, one end of the sampling tube is connected to the sampling hole, and a sealing film is provided at the other end of the sampling tube.

2. The simple stratified sampling centrifuge tube according to claim 1, characterized in that: The sealing film is elastic and is provided with a sampling incision.

3. The simple stratified sampling centrifuge tube according to claim 2, characterized in that: The sampling tube is arranged obliquely, and the sealing film is arranged horizontally.

4. The simple stratified sampling centrifuge tube according to claim 3, characterized in that: The sampling hole is arranged obliquely, and the axis of the sampling hole coincides with the axis of the sampling tube.

5. The simple stratified sampling centrifuge tube according to claim 1, characterized in that: The sealing plug comprises an elastic sealing portion, which is tightened in the sampling hole to achieve a detachable connection between the sealing plug and the sampling hole.

6. The simple stratified sampling centrifuge tube according to claim 5, characterized in that: The sealing plug also includes a hard limiting portion arranged outside the elastic sealing portion, and the size of the hard limiting portion is larger than the size of the elastic sealing portion.

7. The simple stratified sampling centrifuge tube according to claim 6, characterized in that: The hard limiting portion is arc-shaped and can fit with the tube body.

8. The simple stratified sampling centrifuge tube according to any one of claims 5 to 7, characterized in that: A connecting rope is provided between the sealing plug and the connecting strip, and the length of the connecting rope is greater than the distance between the sealing plug and the connecting strip.

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