Continuous organ tissue oxygen partial pressure detection device and system for liver oxygenation mechanical perfusion

Abstract

The utility model discloses a continuous organ tissue oxygen partial pressure detection device and a continuous organ tissue oxygen partial pressure detection system for liver oxygenation mechanical perfusion. The device comprises a layer of metalloporphyrin embedded film which is flatly paved on the surface of a liver, a first laser light source; the optical glass is used for focusing the first laser light source and irradiating the first laser light source onto the fluorescent sensitive material of the film; the filtering and focusing optical assembly is arranged on an excited fluorescent light path; an optical sensor used for receiving laser output by the filtering and focusing optical assembly is connected to the control module. The system comprises a control panel, a power supply system, an oxygenator, a liquid accumulator, an optical sensor for detecting oxygen partial pressure, a flow control motor, a peristaltic pump, a filter, an oxygen generator and a gas flow control unit. The device can be used for monitoring the oxygen level in the liver mechanical perfusion period, can be independent of the existence of an oxygen carrier, and can be used for keeping the oxygen level at a target value, so that the mechanical perfusion effect is optimized, and the damage to the perfused liver caused by oxidative stress reaction is reduced.

Description

[0001] This application is a divisional application of the application with application date of July 6, 2023, application number 202321771217.3, and invention name as continuous organ tissue oxygen partial pressure detection device and system for liver oxygenation mechanical perfusion. Technical Field

[0002] The utility model relates to the field of medical instruments, in particular to a continuous organ tissue oxygen partial pressure detection device and system for liver oxygenation mechanical perfusion. Background Art

[0003] Machine perfusion is increasingly used in the field of preservation of solid organs and vascular composite allotransplantation (VCA). Oxygenation perfusion has become a new clinical need based on traditional mechanical perfusion methods. A large amount of clinical data proves the importance of oxygen for the preservation of ex vivo organs, especially the liver, lungs and heart. Even in a low-temperature and low-metabolism state, sufficient oxygen consumption is still required. However, excessive oxygen partial pressure will also have a negative impact on tissues, causing the generation rate of oxygen free radicals to accelerate and the amount of production to exceed the clearance capacity of the tissue antioxidant system, thereby damaging tissue cells and causing pathological changes. The degree of damage to tissue cells is related to oxygen partial pressure and duration. Therefore, it is necessary to monitor the oxygen of mechanically perfused organs to ensure maximum post-transplant survival and minimal ischemia-reperfusion injury.

[0004] Currently, there is no available means of continuous oxygen partial pressure measurement for decellularized hypothermic perfusion systems. Compared with normothermic mechanical perfusion, because artificial blood or plasma is used, continuous measurement can be performed using an oxygen saturation sensor. However, for hypothermic mechanical perfusion, there is currently no solution for oxygen partial pressure monitoring. Utility Model Content

[0005] The utility model aims to provide a continuous organ tissue oxygen partial pressure detection device and system for liver oxygenation mechanical perfusion, wherein the perfusion system can automatically adjust the oxygen concentration and flow rate when the oxygen level exceeds the safety upper limit.

[0006] In order to solve the above technical problems, the embodiment of the utility model provides a continuous organ tissue oxygen partial pressure detection device for liver oxygenation mechanical perfusion, which comprises:

[0007] A thin film of metalloporphyrin embedded in the liver surface;

[0008] a first laser light source for emitting laser light in the direction of the film;

[0009] Optical glass for focusing the first laser light source and irradiating it onto the fluorescent sensitive material of the film;

[0010] A filtering and focusing optical component disposed on the optical path of the excited fluorescent light, comprising a filter for filtering out stray light and an optical lens for focusing and increasing light intensity;

[0011] The optical sensor is used for receiving the laser output by the filtering and focusing optical component and is connected to the control module.

[0012] The utility model also provides a continuous organ tissue oxygen partial pressure detection system for liver oxygenation mechanical perfusion, which includes a control panel, a power supply system, an oxygenator, an accumulator, an optical sensor for oxygen partial pressure detection, a flow control motor, a peristaltic pump, a filter, an oxygen concentrator, and a gas flow control unit;

[0013] The power supply system, flow control motor and peristaltic pump are connected to the control board;

[0014] The oxygen concentrator is connected to the gas flow control unit, the gas flow control unit is connected to the oxygenator, the oxygenator is connected to the liquid accumulator, and the output end of the liquid accumulator has a first delivery pipeline, a second delivery pipeline, and a third delivery pipeline;

[0015] The first delivery pipeline of the effusion device is connected to the liver portal vein in the organ tank, and the first delivery pipeline is equipped with a first flow rate sensor, a first pinch valve, a first bubble sensor, and a first pressure sensor, and the first flow rate sensor, the first pinch valve, the first bubble sensor, and the first pressure sensor are connected to the control board;

[0016] The second delivery pipeline of the effusion device is connected to the liver artery in the organ tank, and the second delivery pipeline is equipped with a second flow rate sensor, a second pinch valve, a second bubble sensor, and a second pressure sensor, and the second flow rate sensor, the second pinch valve, the second bubble sensor, and the second pressure sensor are connected to the control board;

[0017] The third delivery pipeline of the effusion device is connected to the organ tank, the third delivery pipeline is equipped with a third pinch valve, and the third pinch valve is connected to the control panel;

[0018] The liver surface is flattened with a thin film embedded with metalloporphyrins, and the optical sensor is set on the film;

[0019] The perfusion liquid delivery pipeline of the organ tank is connected to the peristaltic pump, the peristaltic pump is connected to the filter, and the filter is connected to the oxygenator.

[0020] Furthermore, the power supply system includes a power supply management module, which is connected to the AC to DC conversion module; and the power supply management module is connected to the rechargeable battery.

[0021] Furthermore, the control panel is connected to a display and a control panel.

[0022] Furthermore, inlet and outlet temperature detection sensors are provided at the organ tank, and the temperature detection sensors are connected to the controller.

[0023] Furthermore, a third pressure sensor is provided in the connection pipeline between the peristaltic pump and the filter, and the third pressure sensor is connected to the control board.

[0024] The utility model has the following beneficial effects: the utility model can be used to monitor the oxygen level during mechanical perfusion of the liver, can be independent of the presence of an oxygen carrier (blood or artificial), and can be used to maintain the oxygen level at a target value to further optimize the effect of mechanical perfusion and reduce the damage to the perfused liver caused by oxidative stress response. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a schematic diagram of a continuous organ tissue oxygen partial pressure detection device for liver oxygenation mechanical perfusion in the first embodiment of the utility model.

[0026] Figure 2 This is a schematic diagram of a continuous organ tissue oxygen partial pressure detection system for liver oxygenation mechanical perfusion in the second embodiment of the utility model. DETAILED DESCRIPTION

[0027] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific embodiments.

[0028] The first embodiment of the utility model provides a continuous organ tissue oxygen partial pressure detection device for liver oxygenation mechanical perfusion, see Figure 1 It includes a thin film 1 embedded with metal porphyrin spread on the surface of the liver. The thin film is used as a consumable. After the thin film is laid, a first laser light source 3 for emitting laser light in the direction of the thin film is required, such as a blue LED light source. The blue LED light source emits laser light in the direction of the liver, and the optical glass 2 for focusing the laser light source and irradiating it onto the fluorescent sensitive substance of the thin film is arranged on the light path between the thin film and the first laser light source, and can be distributed roughly close to the thin film.

[0029] It is worth mentioning that the device also has filtering and focusing optical components, which are installed on the excited fluorescent light path. Figure 1 The filtering and focusing optical component includes a filter 4 for filtering out stray light and an optical lens 5 for focusing and increasing light intensity, and an optical sensor 6 for receiving the laser output by the filtering and focusing optical component receives the optical signal and outputs it to the control module. The control module sends corresponding instructions to the corresponding device according to the parameters through the received optical signal.

[0030] During mechanical perfusion, the film is laid flat on the surface of the liver to be perfused, and an optical sensor is placed on the film to excite and detect changes in the film's phosphorescence lifetime, which must be calibrated before use to produce tissue oxygen partial pressure (pO2) readings.

[0031] The working process of this device is as follows: the laser is focused through the optical glass window of the sensor and irradiated onto the fluorescent sensitive material containing the metal porphyrin film, stimulating red fluorescence of a certain wavelength. The fluorescence is quenched by oxygen in the liver at the place where the sensitive film contacts the liver skin, causing the fluorescence intensity to decrease and the fluorescence lifetime to weaken. The excited fluorescence is filtered out by the condenser to remove stray light, and the light intensity is increased by the focal length of the optical lens. It is then received by the optical sensor (photoelectric detector) and converted into a voltage signal, which is read by the control module (main control unit). After calibration, the oxygen partial pressure can be continuously measured.

[0032] The second embodiment of the utility model provides a continuous organ tissue oxygen partial pressure detection system for liver oxygenation mechanical perfusion, see Figure 2 , which includes a control board, a power system, an oxygenator, an accumulator, an optical sensor for oxygen partial pressure detection, a flow control motor, a peristaltic pump, a filter, an oxygen concentrator, and a gas flow control unit (such as a flow control valve, etc.).

[0033] In this embodiment, the power supply system as the system power supply unit includes a power management module, and the power management module is connected to the AC to DC module, so that the municipal power supply system can directly supply power. The AC power supply supplies power to the system through the AC to DC module. Considering the wide range of applicable scenarios, the power management module is connected to a rechargeable battery so that the rechargeable battery can be used for power supply in scenarios where there is no municipal power interface.

[0034] See also Figure 1 ,Here the power supply system, flow control motor, and peristaltic pump are connected to the control board, which serves as the control center of the system.

[0035] exist Figure 1In the invention, the oxygen concentrator is connected to a gas flow control unit, such as a flow control valve, the gas flow control unit is connected to an oxygenator, the oxygenator is connected to a fluid accumulator, the output end of the fluid accumulator has a first delivery pipeline, a second delivery pipeline, and a third delivery pipeline. The system has a perfusion fluid circuit, and the three delivery pipelines here are used as components of the circuit. The first delivery pipeline is used to deliver perfusion fluid to the liver portal vein, the second delivery pipeline delivers perfusion fluid to the liver artery, and the third delivery pipeline is used to connect to the organ tank. The third delivery pipeline is used as a bypass. When the perfusion fluid to the liver is stopped, the third delivery pipeline is used to maintain the circulation of the circuit (that is, the third delivery pipeline directly passes the perfusion fluid through the organ tank and then flows out through the perfusion fluid output pipeline of the organ tank). The first delivery pipeline of the fluid accumulator is connected to the liver portal vein in the organ tank, and the first delivery pipeline is installed with a first flow rate sensor, a first pinch valve, a first bubble sensor, and a first pressure sensor. The first flow rate sensor, the first pinch valve, the first bubble sensor, and the first pressure sensor are connected to the control panel. The second delivery pipeline of the accumulator is connected to the liver artery in the organ tank, and the second delivery pipeline is equipped with a second flow rate sensor, a second pinch valve, a second bubble sensor, and a second pressure sensor, which are connected to the control board. The third delivery pipeline of the accumulator is connected to the organ tank, and the third delivery pipeline is equipped with a third pinch valve, which is connected to the control board. The above sensors detect their respective signals and feed them back to the control board, and the control board (in conjunction with various sensors) is used to complete parameter detection of the entire system, thereby improving the level of intelligence and ensuring the reliability and stability of the system.

[0036] It is worth mentioning that the surface of the liver is paved with a thin film embedded with metal porphyrin, and the optical sensor is arranged on the thin film. The device provided in the first embodiment can be used here, which will not be described in detail here.

[0037] Continue to see Figure 1 The perfusion liquid delivery pipeline of the organ tank is connected to the peristaltic pump, the peristaltic pump is connected to the filter, and the filter is connected to the oxygenator. The connecting pipeline between the peristaltic pump and the filter is provided with a third pressure sensor, and the third pressure sensor is connected to the control board.

[0038] Considering the convenience of interaction and use, the control panel is connected to a display and a control panel. In addition, the organ tank is provided with an inlet and outlet temperature detection sensor, that is, the inlet and outlet of the organ tank are respectively provided with a temperature detection sensor to measure the temperature parameters of the inlet and outlet perfusion fluid of the organ tank, respectively. The temperature detection sensor is connected to the controller (i.e., the control panel), and the control panel is provided with multiple USB interfaces.

[0039] The liquid accumulator is provided with a bubble catcher and a liquid level detector, and the liquid level detector is connected to the control board. The liquid accumulator is provided with a third temperature detection sensor (ie, the temperature detection sensor T3 in the figure).

[0040] The utility model uses the signal obtained by the device provided by the first embodiment to allow the oxygen supply system to inject oxygen into the perfusion fluid in time when the liver needs oxygenation, so as to avoid adverse effects on the liver organ.

[0041] Those skilled in the art will appreciate that the above-mentioned embodiments are specific examples for implementing the present invention, and in actual applications, various changes may be made thereto in form and detail without departing from the spirit and scope of the present invention.

Claims

1. A continuous organ tissue oxygen partial pressure detection system for liver oxygenation mechanical perfusion, characterized in that: It includes a control board, a power system, an oxygenator, an accumulator, an optical sensor for oxygen partial pressure detection, a flow control motor, a peristaltic pump, a filter, an oxygen concentrator, and a gas flow control unit; The power supply system, flow control motor and peristaltic pump are connected to the control board; The oxygen concentrator is connected to a gas flow control unit, the gas flow control unit is connected to an oxygenator, the oxygenator is connected to a liquid accumulator, and the output end of the liquid accumulator has a first delivery pipeline, a second delivery pipeline, and a third delivery pipeline; The first delivery pipeline of the effusion device is connected to the liver portal vein in the organ tank, and the first delivery pipeline is equipped with a first flow rate sensor, a first pinch valve, a first bubble sensor, and a first pressure sensor, and the first flow rate sensor, the first pinch valve, the first bubble sensor, and the first pressure sensor are connected to the control board; The second delivery pipeline of the effusion device is connected to the liver artery in the organ tank, and the second delivery pipeline is equipped with a second flow rate sensor, a second pinch valve, a second bubble sensor, and a second pressure sensor, and the second flow rate sensor, the second pinch valve, the second bubble sensor, and the second pressure sensor are connected to the control board; The third delivery pipeline of the effusion device is connected to the organ tank, the third delivery pipeline is installed with a third pinch valve, and the third pinch valve is connected to the control panel; The surface of the liver is paved with a thin film embedded with metal porphyrin, and the optical sensor is arranged on the thin film; The perfusion liquid delivery pipeline of the organ tank is connected to the peristaltic pump, the peristaltic pump is connected to the filter, and the filter is connected to the oxygenator.

2. The continuous organ tissue oxygen partial pressure detection system for liver oxygenation mechanical perfusion according to claim 1, characterized in that: The power supply system includes a power management module, and the power management module is connected to the AC to DC conversion module; The power management module is connected to a rechargeable battery.

3. The continuous organ tissue oxygen partial pressure detection system for liver oxygenation mechanical perfusion according to claim 1, characterized in that: The control panel is connected with a display and a control panel.

4. The continuous organ tissue oxygen partial pressure detection system for liver oxygenation mechanical perfusion according to claim 1, characterized in that: The organ tank is provided with inlet and outlet temperature detection sensors, and the temperature detection sensors are connected to the controller.

5. The continuous organ tissue oxygen partial pressure detection system for liver oxygenation mechanical perfusion according to claim 1, characterized in that: The connecting pipeline between the peristaltic pump and the filter is provided with a third pressure sensor. The third pressure sensor is connected to the control board.

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