Treatment system for 5-aminolevulinic acid phosphate
By designing a treatment system including a liquid slag separator and a multi-stage membrane filter, the waste liquid problem caused by ion exchange column purification in the prior art is solved, and the efficient and environmentally friendly 5-aminolevulinic acid phosphate is achieved. 制备,制得高质量和高收率的成品。
Patent Information
- Application Number
- CN202420920358.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-29
- Publication Date
- 2025-02-14
- Estimated Expiration
- 2034-04-29
AI Technical Summary
In the process of industrial and large-scale preparation of 5-aminolevulinic acid phosphate, the purification of ion exchange columns results in the generation of large amounts of waste liquid, causing environmental pollution and increasing wastewater treatment costs.
A treatment system is designed, including a liquid reservoir, a liquid slag separator, a multi-stage membrane filter, a reactor, a concentration device and a crystallization tank, and solid-liquid separation and step-by-step through a liquid slag separator and a multi-stage membrane filter. Separate, instead of ion exchange columns, reduce wastewater generation.
The system effectively removes bacteria, proteins and macromolecular organic matter in the fermentation broth through a multi-stage membrane filter, reduces membrane damage, improves treatment efficiency, reduces wastewater, and finally produces high-quality and high-yield 5-aminoacetyl Finished product of propionic acid phosphate.
Smart Images
Figure CN222468640U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of bioengineering, and particularly relates to a processing system for 5-aminolevulinic acid phosphate. Background Art
[0002] 5-Aminolevulinic acid phosphate is mainly used to treat respiratory diseases such as chronic bronchitis, emphysema and acute bronchitis. It can also be used for liver detoxification and treatment of alcohol poisoning. It has a wide range of uses and important significance in the medical field.
[0003] In the prior art, 5-aminolevulinic acid phosphate is mainly obtained by acidification of 5-aminolevulinic acid and phosphoric acid. The production equipment of 5-aminolevulinic acid phosphate mainly includes a fermentation tank, a plate-and-frame filter, an ion exchange column, an acidification tank, a concentration crystallization tank, etc. The fermentation tank is mainly used to obtain a fermentation liquid containing 5-aminolevulinic acid by fermentation with Corynebacterium glutamicum as a fermentation strain and glycine as a substrate, and then the fermentation liquid is filtered by a plate-and-frame filter, purified by an ion exchange column, and a 5-aminolevulinic acid desorption liquid is obtained, and then 5-aminolevulinic acid and phosphoric acid are acidified in an acidification tank to obtain 5-aminolevulinic acid phosphate, and finally the 5-aminolevulinic acid phosphate finished product is obtained by concentration crystallization. Since the prior art usually uses an ion exchange column for purification, a large amount of waste liquid will be generated during the purification process of the ion exchange column, thereby causing environmental pollution and increasing the subsequent sewage treatment costs. Utility Model Content
[0004] In view of the above technical problems, the utility model provides a processing system for 5-aminolevulinic acid phosphate, which is used to solve one of the technical problems faced in the process of industrial and large-scale preparation of 5-aminolevulinic acid phosphate.
[0005] The purpose of this utility model is mainly achieved through the following technical solutions:
[0006] On the one hand, the utility model provides a processing system for 5-aminolevulinic acid phosphate, comprising a liquid storage tank, a liquid-slag separator, a multi-stage membrane filter, a reactor, a concentrating device and a crystallizing tank; the discharge port of the liquid storage tank is connected with the feed port of the liquid-slag separator through a pipeline, the liquid discharge port of the liquid-slag separator is connected with the feed port of the multi-stage membrane filter through a pipeline, the clear liquid outlet of the multi-stage membrane filter is connected with the feed port of the reactor through a pipeline, the discharge port of the reactor is connected with the feed port of the concentrating device through a pipeline, the concentrated liquid outlet of the concentrating device is connected with the feed port of the crystallizing tank through a pipeline, and a crystal discharge port is provided at the bottom of the crystallizing tank.
[0007] Furthermore, a first feeding port and a second feeding port are provided on the top of the liquid storage tank, the first feeding port is used to add fermentation liquid containing 5-aminolevulinic acid into the liquid storage tank, the second feeding port is used to add additives into the liquid storage tank, and a fermentation liquid discharge port is provided at the bottom of the liquid storage tank.
[0008] Furthermore, the liquid storage tank includes a tank body, a first pH sensing circuit, a temperature sensing circuit, a liquid level sensing circuit and a control circuit. The pH sensor of the first pH sensing circuit is arranged at the bottom of the tank body, the temperature sensor of the temperature sensing circuit is arranged at the bottom of the tank body, the liquid level sensor of the liquid level sensing circuit is arranged in the tank body, and the output end of the first pH sensing circuit, the output end of the temperature sensing circuit and the output end of the liquid level sensing circuit are all electrically connected to the control circuit.
[0009] Furthermore, the multi-stage membrane filter includes a ceramic membrane filter with a filtration pore size of 50-200nm, an ultrafiltration membrane filter with a filtration pore size of 5000-10000Da, an ultrafiltration membrane filter with a filtration pore size of 1000-5000Da, and an ultrafiltration membrane filter with a filtration pore size of 300-500Da, which are connected in sequence to remove bacteria, proteins and macromolecular organic matter in the fermentation broth.
[0010] Furthermore, a reverse osmosis membrane is arranged between the multistage membrane filter and the reactor, the clear liquid outlet of the multistage membrane filter is connected to the feed port of the reverse osmosis membrane through a pipeline, and the retentate of the reverse osmosis membrane is connected to the feed port of the reactor through a pipeline.
[0011] Furthermore, a feed port and a filling port are correspondingly arranged on the upper side wall of the reactor, a discharge port is arranged at the bottom of the reactor, and a stirring rod extending downward is also arranged at the top of the reactor.
[0012] Furthermore, a decolorization tank is arranged between the reactor and the concentrating device, a filling port is arranged on the top of the decolorization tank, and the decolorization tank is used to store the reaction liquid and decolorize the reaction liquid; the discharge port of the reactor is connected to the feed port of the decolorization tank through a pipeline, and the discharge port of the decolorization tank is connected to the feed port of the concentrating device through a pipeline.
[0013] Furthermore, a feed port is provided on the side wall of the crystallization tank, and a filling port is provided on the top of the crystallization tank.
[0014] Furthermore, a valve is provided on the pipeline.
[0015] Furthermore, a transmission pump is also arranged on the pipeline.
[0016] Compared with the prior art, the utility model can achieve at least one of the following technical effects:
[0017] (1) The treatment system for 5-aminolevulinic acid phosphate proposed in the utility model first performs preliminary solid-liquid separation on the fermentation liquid in the liquid storage tank through a liquid-slag separator to obtain a primary screening liquid, and then uses a multi-stage membrane filter to separate the primary screening liquid step by step, which can grade and remove bacteria, proteins and macromolecular organic matter in the fermentation liquid, is not easy to block the membrane pores, and the production process causes little damage to the membrane and is easy to regenerate. In addition, the membrane treatment liquid has the advantages of large processing capacity and high separation efficiency, which can replace the expensive resin exchange column in the prior art and reduce a lot of regeneration wastewater. The clear liquid filtered by the multi-stage membrane filter is then reacted with phosphoric acid in the reactor to obtain 5-aminolevulinic acid phosphate. After being treated by a concentration device and a crystallization tank, a high-quality and high-yield 5-aminolevulinic acid phosphate finished product can be finally obtained. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation on the present invention. In the drawings:
[0019] Figure 1 A diagram of a processing system for 5-aminolevulinic acid phosphate provided in an embodiment of the present utility model.
[0020] Figure numerals: 1 is a liquid storage tank, 11 is a first pipeline, 12 is a first transmission pump, 13 is a first valve, 2 is a liquid-slag separator, 21 is a second pipeline, 22 is a second transmission pump, 23 is a second valve, 3 is a multi-stage membrane filter, 31 is a third pipeline, 32 is a third transmission pump, 33 is a third valve, 4 is a reactor, 41 is a fourth pipeline, 42 is a fourth transmission pump, 43 is a fourth valve, 5 is a concentration device, 51 is a fifth pipeline, 52 is a fifth transmission pump, 53 is a fifth valve, and 6 is a crystallization tank. DETAILED DESCRIPTION
[0021] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.
[0022] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.
[0023] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present utility model, the meaning of "multiple" is two or more, unless otherwise clearly and specifically defined. The meaning of "several" is one or more, unless otherwise clearly and specifically defined.
[0024] In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as a limitation on the present invention.
[0025] In the description of the present utility model, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.
[0026] A treatment system for 5-aminolevulinic acid phosphate is further described in detail below in conjunction with specific examples. These examples are only used for comparison and explanation purposes, and the present utility model is not limited to these examples.
[0027] See also Figure 1 The treatment system for 5-aminolevulinic acid phosphate provided by the utility model comprises a liquid storage tank 1, a liquid-slag separator 2, a multi-stage membrane filter 3, a reactor 4, a concentrator 5 and a crystallizer 6. For example, the liquid-slag separator 2 can be a bag filter. Among them, the discharge port of the liquid storage tank 1 is connected to the feed port of the liquid-slag separator 2 through a first pipeline 11, the liquid discharge port of the liquid-slag separator 2 is connected to the feed port of the multi-stage membrane filter 3 through a second pipeline 21, the clear liquid outlet of the multi-stage membrane filter 3 is connected to the feed port of the reactor 4 through a third pipeline 31, the discharge port of the reactor 4 is connected to the feed port of the concentrator 5 through a fourth pipeline 41, the concentrated liquid outlet of the concentrator 5 is connected to the feed port of the crystallizer 6 through a fifth pipeline 51, and a crystal discharge port is provided at the bottom of the crystallizer 6.
[0028] During the specific implementation, the operator passes the fermentation liquid into the liquid storage tank 1, performs preliminary solid-liquid separation on the fermentation liquid in the liquid storage tank 1 through the liquid-slag separator 2 to obtain the primary screening liquid, and then uses the multi-stage membrane filter 3 to separate the primary screening liquid step by step. The clear liquid filtered by the multi-stage membrane filter 3 is reacted with phosphoric acid in the reactor 4 to obtain 5-aminolevulinic acid phosphate, and is processed by the concentrator 5 and the crystallizer 6 to finally obtain a high-quality and high-yield 5-aminolevulinic acid phosphate finished product.
[0029] Through the structure and specific implementation process of the above-mentioned treatment system, it can be known that the treatment system for 5-aminolevulinic acid phosphate proposed by the utility model first performs preliminary solid-liquid separation on the fermentation liquid in the liquid storage tank 1 through the liquid-slag separator 2 to obtain the primary screening liquid, and then uses the multi-stage membrane filter 3 to separate the primary screening liquid step by step, which can grade and remove the bacteria, protein and macromolecular organic matter in the fermentation liquid, is not easy to block the membrane pores, and the production process has little damage to the membrane and is easy to regenerate. In addition, the membrane treatment feed liquid has the advantages of large processing capacity and high separation efficiency, which can replace the expensive resin exchange column in the prior art and reduce a lot of regeneration wastewater. The clear liquid filtered by the multi-stage membrane filter 3 is reacted with phosphoric acid in the reactor 4 to obtain 5-aminolevulinic acid phosphate, which is treated by the concentrator 5 and the crystallizer 6, and finally a high-quality and high-yield 5-aminolevulinic acid phosphate finished product can be obtained.
[0030] As a possible implementation method, the liquid storage tank 1 of the treatment system in this embodiment can be provided with a first feeding port and a second feeding port, the first feeding port is used to add the fermentation liquid containing 5-aminolevulinic acid into the liquid storage tank, and the second feeding port is used to add additives into the liquid storage tank 1. By way of example, the additive can be a pH adjuster, etc. A fermentation liquid discharge port is provided at the bottom of the liquid storage tank.
[0031] As a possible implementation, the liquid storage tank 1 of the treatment system in this embodiment includes a tank body, a pH sensing circuit, a temperature sensing circuit, a liquid level sensing circuit and a control circuit. For example, the liquid level sensor can be a float type liquid level sensor. The pH sensor of the pH sensing circuit is arranged at the bottom of the tank body, the temperature sensor of the temperature sensing circuit is arranged at the bottom of the tank body, the liquid level sensor of the liquid level sensing circuit is arranged in the tank body, the output end of the pH sensing circuit, the output end of the temperature sensing circuit and the output end of the liquid level sensing circuit are all electrically connected to the control circuit, the pH sensing circuit can accurately and quickly detect the pH value change in the tank, the temperature sensing circuit can accurately and quickly detect the temperature change in the tank, so as to timely regulate the growth environment of the Corynebacterium glutamicum in the tank, and the liquid level sensing circuit can accurately and quickly detect the liquid level in the tank, so that the operator can know the fermentation status of the fermentation liquid.
[0032] As a possible implementation method, the multi-stage membrane filter 3 of the treatment system in this embodiment includes a ceramic membrane filter with a filtration pore size of 50-200nm, an ultrafiltration membrane filter with a filtration pore size of 5000-10000Da, an ultrafiltration membrane filter with a filtration pore size of 1000-5000Da, and an ultrafiltration membrane filter with a filtration pore size of 300-500Da. For example, the connection method of the above-mentioned multi-stage membrane filter can be referred to as follows: the liquid discharge port of the liquid-slag separator is connected to the liquid inlet of the ceramic membrane filter with a filtration pore size of 50-200nm through the second pipeline 21, the clear liquid outlet of the ceramic membrane filter with a filtration pore size of 50-200nm is connected to the liquid inlet of the ultrafiltration membrane filter with a filtration pore size of 5000-10000Da, the clear liquid outlet of the ultrafiltration membrane filter with a filtration pore size of 5000-10000Da is connected to the liquid inlet of the ultrafiltration membrane filter with a filtration pore size of 1000-5000Da, the clear liquid outlet of the ultrafiltration membrane filter with a filtration pore size of 1000-5000Da is connected to the liquid inlet of the ultrafiltration membrane filter with a filtration pore size of 300-500Da, and the clear liquid outlet of the 300-500Da ultrafiltration membrane filter is connected to the feed port of the reactor 4 through the third pipeline 31.
[0033] When the above-mentioned embodiment is used specifically, according to the conventional use conditions of different ceramic membrane filters in the prior art, the fermentation broth is filtered under specified pH and temperature conditions and specified pressure conditions, for example, at a pH of 3.5 to 5, a temperature range of 25 to 30°C, and a pressure of 0.2 to 0.4 MPa to remove bacteria, proteins and macromolecular organic matter in the fermentation broth. The above-mentioned specific parameters can be selected according to the requirements of ultrafiltration membranes with different pore sizes. In this way, bacteria, proteins and macromolecular organic matter in the fermentation broth can be removed in stages, the membrane pores are not easily blocked, the production process causes little damage to the membrane, and it is easy to regenerate.
[0034] As a possible implementation, in the present embodiment, a reverse osmosis membrane (not shown in the accompanying drawings) is further arranged between the multistage membrane filter and the reactor. The clear liquid outlet of the multistage membrane filter is communicated with the feed port of the reverse osmosis membrane by a pipeline (not shown in the accompanying drawings), and the retentate of the reverse osmosis membrane is communicated with the feed port of the reactor by a pipeline. During specific implementation, in the multistage membrane filter that is communicated successively, the minimum filter particle size, that is, the clear liquid outlet of the 300-500Da ultrafiltration membrane filter at the end is first communicated with the feed port of the reverse osmosis membrane by a pipeline, and the retentate of the reverse osmosis membrane is communicated with the feed port of the reactor 4 by the third pipeline 31. By arranging a reverse osmosis membrane, the reaction solution can be concentrated before the clear liquid (i.e., the reaction solution) enters the reactor, so as to reduce the volume of the reaction solution. The specific use conditions of the reverse osmosis membrane can refer to the specific requirements of the reverse osmosis membrane equipment specifically selected.
[0035] As a possible implementation, the upper side wall of the reactor 4 of the treatment system in the present embodiment is correspondingly provided with a feed port and a filling port, the bottom of the reactor 4 is provided with a discharge port, and the top of the reactor 4 is also provided with a stirring rod extending downward, and the filtered clear liquid reacts with phosphoric acid in the reactor 4, and after stirring by the stirring rod of the reactor 4, the reaction is made more sufficient to obtain a 5-aminolevulinic acid phosphate solution. The feed port is used for membrane filtration of the clear liquid, and the filling port is provided to add reagents required in the reaction process, such as pH value regulators, etc., through the filling port, and the specific reaction conditions can refer to the prior art. Of course, a second pH sensing circuit can also be provided in the reactor 4 as needed, and the pH sensor of the second pH sensing circuit is provided at the bottom of the reactor tank body, and the output end of the second pH sensing circuit is also electrically connected to the control circuit, and the reaction conditions required for the conventional 5-aminolevulinic acid and phosphoric acid reaction in the prior art can be added.
[0036] As a possible implementation, a decolorization tank is further provided between the reactor 4 and the concentrator 5 of the treatment system in this embodiment, and a filling port is provided on the top of the decolorization tank, and the filling port is used to add a decolorizer, such as activated carbon, etc. The decolorization tank is used to store the reaction solution and decolorize the reaction solution; the discharge port of the reactor 4 is connected to the feed port of the decolorization tank through a pipeline, and the discharge port of the decolorization tank is connected to the feed port of the concentrator 5 through a pipeline, and the decolorization tank decolorizes the 5-aminolevulinic acid phosphate solution, further purifies the 5-aminolevulinic acid phosphate solution, and thus obtains a high-quality 5-aminolevulinic acid phosphate solution.
[0037] As a possible implementation, the concentrator 5 in this embodiment is conventionally set, mainly used to concentrate the 5-aminolevulinic acid phosphate solution, and the purpose of concentration is to facilitate subsequent crystallization. The concentrated solution after concentration by the concentrator 5 enters the crystallizer 6, and a feed port is provided on the side wall of the crystallizer 6, and a filling port is provided on the top of the crystallizer 6. It should be understood that the feed port on the top of the crystallizer is mainly used to add the concentrated solution into the crystallizer, and the filling port is mainly used to add additives required in the crystallization process, such as the addition of seed crystals.
[0038] As a possible implementation, valves are provided on the pipelines of the processing system in this embodiment, and the valves specifically include: a first valve 13 provided on the first pipeline, a second valve 23 provided on the second pipeline, a third valve 33 provided on the third pipeline, a fourth valve 43 provided on the fourth pipeline, and a fifth valve 53 provided on the fifth pipeline. The valves are mainly used to adjust the flow and stop of the liquid in the pipeline. Of course, the valves can also be provided as a backflow valve with a backflow prevention function, so that the required liquid after transmission can be prevented from backflowing and the equipment safety can be protected.
[0039] As a possible implementation method, a transmission pump is also provided on the pipeline of the processing system in this embodiment, and the transmission pump specifically includes: a first transmission pump 12 arranged on the first pipeline, a second transmission pump 22 arranged on the second pipeline, a third transmission pump 32 arranged on the third pipeline, a fourth transmission pump 42 arranged on the fourth pipeline, and a fifth transmission pump 52 arranged on the fifth pipeline. The transmission pump is mainly used to provide driving force when transmitting the required liquid.
[0040] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by any technician familiar with the technical field within the technical scope disclosed in the present invention should be included in the protection scope of the present invention.
Claims
1. A processing system for 5-aminolevulinic acid phosphate, characterized in that: The invention comprises a liquid storage tank, a liquid-slag separator, a multi-stage membrane filter, a reactor, a concentrator and a crystallizer; the discharge port of the liquid storage tank is communicated with the feed port of the liquid-slag separator through a pipeline, the liquid discharge port of the liquid-slag separator is communicated with the feed port of the multi-stage membrane filter through a pipeline, the clear liquid outlet of the multi-stage membrane filter is communicated with the feed port of the reactor through a pipeline, the discharge port of the reactor is communicated with the feed port of the concentrator through a pipeline, the concentrated liquid outlet of the concentrator is communicated with the feed port of the crystallizer through a pipeline, and a crystal discharge port is arranged at the bottom of the crystallizer.
2. The processing system according to claim 1, characterized in that The top of the liquid storage tank is provided with a first feeding port and a second feeding port, the first feeding port is used to add the fermentation liquid containing 5-aminolevulinic acid into the liquid storage tank, the second feeding port is used to add additives into the liquid storage tank, and the bottom of the liquid storage tank is provided with a fermentation liquid discharge port.
3. The processing system according to claim 1 or 2, characterized in that: The liquid storage tank includes a tank body, a first pH sensing circuit, a temperature sensing circuit, a liquid level sensing circuit and a control circuit. The pH sensor of the first pH sensing circuit is arranged at the bottom of the tank body, the temperature sensor of the temperature sensing circuit is arranged at the bottom of the tank body, the liquid level sensor of the liquid level sensing circuit is arranged in the tank body, and the output end of the first pH sensing circuit, the output end of the temperature sensing circuit and the output end of the liquid level sensing circuit are all electrically connected to the control circuit.
4. The processing system according to claim 1, characterized in that The multi-stage membrane filter comprises a ceramic membrane filter with a filtration pore size of 50-200nm, an ultrafiltration membrane filter with a filtration pore size of 5000-10000Da, an ultrafiltration membrane filter with a filtration pore size of 1000-5000Da, and an ultrafiltration membrane filter with a filtration pore size of 300-500Da, which are connected in sequence and are used to remove bacteria, proteins and macromolecular organic matter in the fermentation liquid.
5. The processing system according to claim 1, characterized in that A reverse osmosis membrane is also arranged between the multi-stage membrane filter and the reactor. The clear liquid outlet of the multi-stage membrane filter is connected to the feed port of the reverse osmosis membrane through a pipeline, and the retentate of the reverse osmosis membrane is connected to the feed port of the reactor through a pipeline.
6. The processing system according to claim 1 or 5, characterized in that: The upper side wall of the reactor is provided with a feed port and a filling port respectively, the bottom of the reactor is provided with a discharge port, and the top of the reactor is also provided with a stirring rod extending downward.
7. The processing system according to claim 1 or 6, characterized in that: A decolorization tank is also provided between the reactor and the concentrating device. A filling port is provided on the top of the decolorization tank. The decolorization tank is used to store the reaction liquid and decolorize the reaction liquid. The discharge port of the reactor is connected to the feed port of the decolorization tank through a pipeline, and the discharge port of the decolorization tank is connected to the feed port of the concentrating device through a pipeline.
8. The processing system according to claim 1, characterized in that A feed port is arranged on the side wall of the crystallization tank, and a filling port is arranged on the top of the crystallization tank.
9. The processing system according to claim 1, characterized in that A valve is arranged on the pipeline.
10. The processing system according to claim 1, characterized in that A transmission pump is also arranged on the pipeline.