Neutrophil gelatinase associated lipocalin determination kit

Abstract

The utility model discloses a neutrophil gelatinase associated lipocalin determination kit, and belongs to the technical field of a lipocalin determination kit. The heat dissipation device comprises a protection box body, a plurality of heat dissipation openings are symmetrically formed in the two sides of the protection box body, the heat dissipation openings are evenly arranged, a heat insulation frame is fixedly connected to the interior of the protection box body, and semiconductor chilling plates are fixedly connected to the two sides of the interior of the heat insulation frame. The temperature of the hot end of the semiconductor chilling plate is reduced in an active heat dissipation mode, the temperature of the cold end can be correspondingly reduced, long-time continuous cooling can be achieved, the sealing shell can be cooled through cooperation of the heat conduction opening, the sealing shell, the refrigerant, the containing groove, the heat conduction column, the box cover and the sealing frame strip, and the service life of the semiconductor chilling plate is prolonged. Furthermore, the reagent can be cooled, heat loss in the protection box body can be reduced, and the heat preservation effect of the protection box body can be improved.

Description

Technical Field

[0001] The utility model belongs to the technical field of a carrier protein determination kit, in particular to a neutrophil gelatinase-related lipocalin determination kit. Background Art

[0002] Neutrophil gelatinase-associated lipocalin (NGAL) is a small molecular weight secretory protein originally discovered in activated neutrophils. Modern studies have shown that NGAL is one of the most effective biological markers for diagnosing acute kidney injury and one of the effective markers for early diabetic nephropathy. Neutrophil gelatinase-associated lipocalin undergoes antigen-antibody reaction with the sensitized latex particles of goat anti-human neutrophil gelatinase-associated lipocalin monoclonal antibody in the reagent in a phosphate buffer system, and agglutinates under the action of the aggregation promoter polyethylene glycol to increase turbidity. The change in absorbance of the reaction solution is detected at a wavelength of 546nm, and the degree of change is proportional to the NGAL content in the sample.

[0003] Traditional original packaged test kits should be stored in a sealed, light-proof condition at 2-8°C with a shelf life of 12 months. After opening the bottle, the reagents are stable for 30 days at 2-8°C. The matching calibrators should be discarded after opening the bottle.

[0004] After searching, Chinese patent application No. 201921447071.0 discloses a neutrophil gelatinase-associated lipocalin assay kit. The patent relates to the field of test kits, specifically to a neutrophil gelatinase-associated lipocalin assay kit, including a box body, a sponge and a box cover. The box body is a cylindrical shell structure, the sponge is arranged on the inner side of the box body, the box cover is closed and arranged at the top opening of the box body, and an interlayer for holding cooling water is provided in the side wall of the box body, the interlayer is connected to the inner side of the box body, and a control compartment is provided at the bottom of the box body. A controller, a semiconductor refrigeration sheet and two sensors for detecting the position of the test kit are provided in the control compartment. The semiconductor refrigeration sheet and the two sensors are electrically connected to the controller. A pressure ring for fitting into the interlayer is provided at the bottom of the box cover. The neutrophil gelatinase-associated lipocalin assay kit of the patent can provide effective protection and buffering for the test kit, and can also automatically perform effective refrigeration treatment on the test kit placed therein.

[0005] Although the above patent can provide effective protection and buffering for the test kit and can also automatically and effectively refrigerate the test kit placed therein, in actual use, the patent adopts semiconductor refrigeration chips for cooling, and the semiconductor refrigeration chips need to dissipate heat at their hot ends during use. However, the semiconductor refrigeration chip placement area of ​​the patent is in a closed state, and the semiconductor refrigeration chip cannot be dissipated, resulting in the cooling effect of the semiconductor refrigeration chip gradually decreasing during use until it is unable to cool down, making the device unable to maintain cooling for a long time.

[0006] Therefore, it is necessary to modify the assay kit in the above patent to effectively prevent the problem that the semiconductor refrigeration chip cannot be cooled, resulting in the cooling effect of the semiconductor refrigeration chip gradually decreasing during use, until it cannot cool down and the device cannot continue to cool down for a long time. Utility Model Content

[0007] In order to solve the problems raised in the above-mentioned background technology, the purpose of the utility model is to provide a neutrophil gelatinase-associated lipid transporter protein assay kit, which has the advantages of heat dissipation treatment for the hot end of the semiconductor refrigeration plate, and is convenient for taking the required reagents, increasing the convenience of taking, and solving the problem that the cooling effect of the semiconductor refrigeration plate during use cannot be gradually reduced until it cannot be cooled, making the device unable to continue cooling for a long time.

[0008] The utility model provides the following technical scheme: a neutrophil gelatinase-associated lipocalin assay kit comprises a protective box body, a plurality of heat dissipation ports are symmetrically provided on both sides of the protective box body, and the plurality of heat dissipation ports are evenly arranged, a thermal insulation frame is fixedly connected to the interior of the protective box body, semiconductor refrigeration sheets are fixedly connected to both sides of the interior of the thermal insulation frame, heat dissipation fans are arranged at the hot ends of both sides of the semiconductor refrigeration sheets, and the heat dissipation fans are fixedly connected to the thermal insulation frame and communicated with the heat dissipation ports, a heat conduction port is provided on the inner wall of the thermal insulation frame, and the other end of the heat conduction port is communicated with the cold end of the semiconductor refrigeration sheet, a sealing shell is fixedly connected to the inner wall of the thermal insulation frame, a refrigerant is arranged inside the sealing shell, a plurality of placement grooves are provided on the top of the sealing shell, and the plurality of placement grooves are evenly arranged, a heat conduction column is fixedly connected to the surface of the sealing shell, and the other end of the heat conduction column passes through the heat conduction port and is arranged correspondingly to the semiconductor refrigeration sheet, a box cover is hingedly connected to the top of the protective box body, a sealing frame strip is fixedly connected to the surface of the box cover, and the sealing frame strip is clamped with the thermal insulation frame.

[0009] The beneficial effects of the utility model are as follows: the utility model uses active heat dissipation to reduce the temperature of the hot end of the semiconductor refrigeration plate, and the temperature of the cold end will also drop accordingly, which can achieve long-term continuous cooling. The utility model uses the cooperation of the heat conduction port, the sealing shell, the refrigerant, the placement groove, the heat conduction column, the box cover and the sealing frame strip to achieve the cooling of the sealing shell, and then the reagent can be cooled, and the heat loss in the protective box can be reduced to improve its insulation effect. The utility model can clamp the reagent through the setting of the support frame, the clamping frame, the clamping port and the clamping ring, which is convenient for subsequent removal and use by the staff. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 It is a schematic diagram of the structure of the utility model.

[0011] Figure 2 It is a schematic diagram of a rear-view stereoscopic structure of the utility model.

[0012] Figure 3 It is a partial cross-sectional stereoscopic schematic diagram of the structure of the utility model when viewed from the front.

[0013] Figure 4 It is a bottom-up stereoscopic cross-sectional schematic diagram of the sealing shell of the utility model.

[0014] Figure 5 It is a stereoscopic schematic diagram of the corresponding arrangement of the clamping ring, the sliding column and the toggle block of the utility model. DETAILED DESCRIPTION

[0015] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0016] like Figures 1 to 5 As shown, the present embodiment is applied to a neutrophil gelatinase-associated lipocalin assay kit, comprising a protective box body 1, a plurality of heat dissipation ports 2 are symmetrically provided on both sides of the protective box body 1, and the plurality of heat dissipation ports 2 are evenly arranged, a thermal insulation frame 3 is fixedly connected to the interior of the protective box body 1, semiconductor cooling sheets 4 are fixedly connected to both sides of the thermal insulation frame 3, heat dissipation fans 5 are provided at the hot ends of both sides of the semiconductor cooling sheets 4, and the heat dissipation fans 5 are fixedly connected to the thermal insulation frame 3 and communicated with the heat dissipation ports 2, a heat conduction port 6 is provided on the inner wall of the thermal insulation frame 3, and the heat conduction port 6 is The other end is connected to the cold end of the semiconductor refrigeration sheet 4, the inner wall of the thermal insulation frame 3 is fixedly connected with a sealing shell 7, a refrigerant 8 is arranged inside the sealing shell 7, a plurality of placement grooves 9 are opened on the top of the sealing shell 7, and the plurality of placement grooves 9 are evenly arranged, a heat-conducting column 10 is fixedly connected to the surface of the sealing shell 7, and the other end of the heat-conducting column 10 passes through the heat-conducting port 6 and is arranged corresponding to the semiconductor refrigeration sheet 4, a box cover 11 is hinged on the top of the protective box body 1, a sealing frame strip 12 is fixedly connected to the surface of the box cover 11, and the sealing frame strip 12 is clamped with the thermal insulation frame 3.

[0017] refer to Figure 3 and Figure 5 A support frame 13 is fixedly connected to the top of the sealing shell 7, and a clamping frame 14 is fixedly connected to the top of the support frame 13. A plurality of bayonet holes 15 are provided inside the clamping frame 14, and the plurality of bayonet holes 15 are respectively in a one-to-one correspondence with the plurality of placement grooves 9, and a clamping ring 16 is clamped inside the bayonet holes 15.

[0018] In this embodiment, the supporting frame 13, the clamping frame 14, the clamping port 15 and the clamping ring 16 are provided to clamp the reagent, so that the reagent can be taken out and used by the staff later.

[0019] refer to Figure 3 and Figure 5 A sliding hole 17 is symmetrically provided at the top of the snap-fit ​​frame 14 about the bayonet 15 , a sliding column 18 is slidably connected inside the sliding hole 17 , and the bottom of the sliding column 18 is fixedly connected to the clamping ring 16 through a connecting piece, and a toggle block 19 is fixedly connected to the top of the sliding column 18 .

[0020] In this embodiment, the sliding hole 17, the sliding column 18 and the toggle block 19 are arranged to facilitate the staff to pull the toggle block 19 upwards, thereby driving the reagent in the clamping ring 16 to move upwards, thereby facilitating the staff to take out the reagent.

[0021] refer to Figure 1 , Figure 3 and Figure 4 A control unit 20 is fixedly connected to the surface of the protective box body 1, and the control unit 20 is electrically connected to the semiconductor refrigeration sheet 4 and the heat dissipation fan 5. A temperature detector 21 is fixedly connected to the surface of the sealing shell 7, and the temperature detector 21 is electrically connected to the control unit 20.

[0022] In this embodiment, the control unit 20 is provided to facilitate the staff to control the various electrical appliances in the measuring kit. The temperature detector 21 is provided to monitor the temperature of the refrigerant 8 to facilitate the staff to adjust the cooling power of the semiconductor refrigeration plate 4.

[0023] refer to Figure 1 A plurality of sponge pressure pads 22 are fixedly connected to the inner wall of the box cover 11 , and the plurality of sponge pressure pads 22 are in a one-to-one correspondence with the plurality of placement slots 9 .

[0024] In this embodiment, a plurality of sponge pressure pads 22 are provided to cover the top of the reagent to prevent it from shaking during transportation.

[0025] refer to Figure 1 A lock body 23 is symmetrically fixedly connected to the front of the protective box body 1, and a lock buckle 24 is symmetrically fixedly connected to the surface of the box cover 11, and the lock buckle 24 is arranged correspondingly to the lock body 23.

[0026] In this embodiment, the lock body 23 and the lock buckle 24 are arranged to lock the protective box body 1 and the box cover 11, thereby improving the sealing effect thereof.

[0027] The utility model opens the box cover 11 through the lock body 23 and the lock buckle 24, then places the reagent inside the clamp ring 16, inserts it into the placement groove 9, then closes the box cover 11 and locks it, and the utility model presets the temperature of the refrigerant 8 through the control unit 20, and the utility model starts the semiconductor refrigeration sheet 4 and the heat dissipation fan 5. At this time, the cold end of the semiconductor refrigeration sheet 4 cools the refrigerant 8 in the sealed shell 7 through the heat-conducting column 10, and then the reagent in the placement groove 9 can be cooled. At this time, the heat dissipation fan 5 can be used to dissipate the heat of the hot end of the semiconductor refrigeration sheet 4. The heat is dissipated outward through the heat dissipation port 2, which can realize long-term continuous cooling of the semiconductor refrigeration plate 4. At this time, the temperature of the refrigerant 8 can be detected in real time by the temperature detector 21. If the temperature of the refrigerant 8 is higher than the preset value, the output power of the cold end of the semiconductor refrigeration plate 4 is increased, and vice versa. When the reagent needs to be taken out, the box cover 11 is opened first. The utility model pulls up the toggle blocks 19 on both sides of the corresponding reagent, which can drive the sliding column 18 to move upward and drive the retaining ring 16 to translate upward, so that the reagent can slide out upward, which is convenient for taking the required reagent and increases the convenience of taking.

Claims

1. A kit for determining neutrophil gelatinase-associated lipocalin, comprising a protective box body (1), characterized in that: A plurality of heat dissipation openings (2) are symmetrically provided on both sides of the protection box body (1), and the plurality of heat dissipation openings (2) are evenly arranged; a heat insulation frame (3) is fixedly connected to the interior of the protection box body (1); semiconductor cooling sheets (4) are fixedly connected to both sides of the interior of the heat insulation frame (3); heat dissipation fans (5) are provided at the hot ends of both sides of the semiconductor cooling sheets (4); the heat dissipation fans (5) are fixedly connected to the heat insulation frame (3) and communicated with the heat dissipation openings (2); a heat conduction opening (6) is provided on the inner wall of the heat insulation frame (3), and the other end of the heat conduction opening (6) is communicated with the cold end of the semiconductor cooling sheet (4); the heat insulation frame The inner wall of the protective box (3) is fixedly connected to a sealing shell (7), a refrigerant (8) is arranged inside the sealing shell (7), a plurality of placement grooves (9) are opened on the top of the sealing shell (7), and the plurality of placement grooves (9) are evenly arranged, a heat-conducting column (10) is fixedly connected to the surface of the sealing shell (7), and the other end of the heat-conducting column (10) passes through the heat-conducting port (6) and is arranged corresponding to the semiconductor refrigeration plate (4), the top of the protective box body (1) is hinged with a box cover (11), the surface of the box cover (11) is fixedly connected with a sealing frame strip (12), and the sealing frame strip (12) is snap-fitted with the thermal insulation frame (3).

2. A neutrophil gelatinase-associated lipocalin assay kit according to claim 1, characterized in that: The top of the sealing shell (7) is fixedly connected to a support frame (13), the top of the support frame (13) is fixedly connected to a snap-on frame (14), a plurality of snap-on openings (15) are provided inside the snap-on frame (14), and the plurality of snap-on openings (15) are respectively in a one-to-one correspondence with the plurality of placement grooves (9), and a snap ring (16) is snap-oned inside the snap-on opening (15).

3. A neutrophil gelatinase-associated lipocalin assay kit according to claim 2, characterized in that: A sliding hole (17) is symmetrically provided on the top of the snap-fit ​​frame (14) with respect to the snap-fit ​​socket (15); a sliding column (18) is slidably connected inside the sliding hole (17); the bottom of the sliding column (18) is fixedly connected to the snap ring (16) via a connecting piece; and a toggle block (19) is fixedly connected to the top of the sliding column (18).

4. A neutrophil gelatinase-associated lipocalin assay kit according to claim 3, characterized in that: A control unit (20) is fixedly connected to the surface of the protective box body (1), and the control unit (20) is electrically connected to the semiconductor refrigeration sheet (4) and the heat dissipation fan (5); a temperature detector (21) is fixedly connected to the surface of the sealing shell (7), and the temperature detector (21) is electrically connected to the control unit (20).

5. A neutrophil gelatinase-associated lipocalin assay kit according to claim 4, characterized in that: A plurality of sponge pressure pads (22) are fixedly connected to the inner wall of the box cover (11), and the plurality of sponge pressure pads (22) are in a one-to-one correspondence with the plurality of placement grooves (9).

6. A neutrophil gelatinase-associated lipocalin assay kit according to claim 1, 2 or 5, characterized in that: A lock body (23) is symmetrically fixedly connected to the front of the protection box body (1), and a lock buckle (24) is symmetrically fixedly connected to the surface of the box cover (11), and the lock buckle (24) and the lock body (23) are arranged in a corresponding manner.

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