Vacuum filtration nucleic acid extraction device

Abstract

The utility model provides a vacuum filtration nucleic acid extraction device, which comprises a vacuum box, a vacuum pump and a non-contact connecting cylinder, the top of the vacuum box is connected with the non-contact connecting cylinder through a plurality of connecting holes, the vacuum box is fixedly provided with the vacuum pump for vacuumizing the vacuum box, and the vacuum pump is connected with the non-contact connecting cylinder through a plurality of connecting holes. A waste liquid opening is formed in the lower portion of the side wall of the vacuum box. The liquid adding-centrifuging-waste liquid pouring circulating operation can be omitted, the consumption of experimental consumables can be effectively reduced, experimental garbage can be reduced, the experimental efficiency can be improved, and the device can be popularized and used.

Description

Technical Field

[0001] The utility model relates to a suction filtration device, in particular to a vacuum suction filtration nucleic acid extraction device. Background Art

[0002] Nucleic acid extraction and protein separation and purification are important operating steps in molecular biology. In the prior art, solid phase purification is generally used for nucleic acid extraction and protein separation and purification operations, and impurities are intercepted by solid phase adsorption media, which generally includes four steps: cell lysis, nucleic acid adsorption, washing and elution.

[0003] In the prior art, during the nucleic acid extraction process, it is often necessary to remove the plug on the nucleic acid test tube, then insert the connector, and then insert the adsorption column on the connector for extraction. Such connector and adsorption column are often connected in a contact manner and cannot be reused. Moreover, during use, when processing different numbers of samples, it is necessary to connect and exchange between the plug and the connector, which can easily cause mutual contamination between samples. For example, a non-contact connection device for nucleic acid extraction disclosed in announcement number CN218951368 U only discloses the connection part, without disclosing the entire system. Utility Model Content

[0004] The technical problem to be solved by the utility model is to provide a vacuum filtration nucleic acid extraction device in view of the deficiencies of the above-mentioned prior art, which can save the liquid adding-centrifugation-waste liquid pouring cycle operation, effectively reduce the consumption of experimental consumables, reduce experimental waste, improve experimental efficiency, and can be promoted for use.

[0005] In order to solve the above technical problems, the technical solution adopted by the utility model is: a vacuum filtration nucleic acid extraction device, characterized in that it includes a vacuum box, a vacuum pump and a non-contact connecting cylinder, the top of the vacuum box is connected to the non-contact connecting cylinder through a plurality of connecting holes, a vacuum pump for evacuating the vacuum box to a vacuum is fixedly arranged on the vacuum box, and a waste liquid port is arranged at the lower part of the side wall of the vacuum box.

[0006] Preferably, the non-contact connection cylinder comprises a connection tube and an adsorption column, the lower end of the connection tube is fixedly connected with a connection head, the outer side of the adsorption column is provided with a connection part, the adsorption column is threadedly connected to the connection tube through the connection part, the upper end of the adsorption column is provided with a first plugging cover, the lower end of the adsorption column is provided with an adsorption port, the adsorption port extends into the inner side of the connection tube, the back side of the connection tube is fixedly connected with a second plugging cover, the front side of the second plugging cover is provided with a rubber ring, the back side of the second plugging cover is provided with a marking mechanism, the inner side of the lower end of the connection head is provided with a There is a second sealing ring, a one-way valve is provided on the inner side of the connecting pipe, a threaded groove is provided on the upper end of the connecting pipe, the adsorption column is threadedly installed through the connecting part and the threaded groove, a first sealing ring is provided on the inner side of the threaded groove, a peeling block is fixedly connected to the upper end of the second blocking cover, the marking mechanism includes a marking groove, the marking groove is provided on the back side of the second blocking cover, a soft magnetic layer is adhesively connected to the inner side of the marking groove, an iron sheet is magnetically connected to the outer side of the soft magnetic layer, a label paper is adhesively connected to the outer side of the iron sheet, and an arc groove is provided at the notch of the marking groove.

[0007] Compared with the prior art, the utility model has the following advantages:

[0008] 1. The utility model has a scientific and reasonable structural design, can connect multiple non-contact connection tubes at the same time, improve the efficiency of nucleic acid extraction and filtration, reduce consumables consumption and research costs, and can be promoted for use.

[0009] 2. When processing different sample quantities, the utility model does not need to connect and interchange between the plug and the single connection end, which simplifies the use process, saves time, improves work efficiency, can be used repeatedly, and reduces waste.

[0010] The utility model is further described in detail below with reference to the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 It is a structural schematic diagram of the utility model.

[0012] Figure 2 It is a structural schematic diagram of the non-contact connecting cylinder in the utility model.

[0013] Figure 3 is the nucleic acid quality of corn, rice and wheat in this embodiment (the green area indicates that the nucleic acid quality is qualified, the yellow area indicates that the nucleic acid quality is unqualified, corn is 1-10, rice is 11-20, and wheat is 21-30)

[0014] Figure 4 Schematic diagram of nucleic acid concentrations of corn, rice and wheat in this example.

[0015] Figure 5 It is the electrophoresis diagram of this example.

[0016] Figure 6 This is the standard gene amplification curve in corn in this example.

[0017] Figure 7 It is the standard gene amplification curve of rice in this embodiment.

[0018] Figure 8 This is the amplification curve of the wheat internal standard gene in this embodiment.

[0019] Description of reference numerals:

[0020] 1—Non-contact connection cylinder; 2—Vacuum box; 3—Vacuum pump;

[0021] 4—waste liquid port; 1-1—connector; 1-2—connecting tube;

[0022] 1-3—adsorption column; 1-4—adsorption port; 1-5—first plugging cover;

[0023] 1-6—connecting part; 1-7—thread groove; 1-8—first sealing ring;

[0024] 1-9—Second plug cover; 1-10—Rubber ring; 1-11—Removal block;

[0025] 1-14—One-way valve; 1-15—Second sealing ring. DETAILED DESCRIPTION

[0026] like Figure 1 and Figure 2 As shown, the utility model includes a vacuum box 2, a vacuum pump 3 and a non-contact connecting tube 1. The top of the vacuum box 2 is connected to the non-contact connecting tube 1 through 20 connecting holes. The vacuum box 2 is fixedly provided with a vacuum pump 3 for evacuating the vacuum box 2 to a vacuum. The lower part of the side wall of the vacuum box 2 is provided with a waste liquid port 4.

[0027] In this embodiment, the non-contact connection tube 1 includes a connection tube 1-2 and an adsorption column 1-3, the lower end of the connection tube 1-2 is fixedly connected with a connection head 1-1, the outer side of the adsorption column 1-3 is provided with a connection part 1-6, the adsorption column 1-3 is threadedly connected to the connection tube 1-2 through the connection part 1-6, the upper end of the adsorption column 1-3 is provided with a first plugging cover 1-5, the lower end of the adsorption column 1-3 is provided with an adsorption port 1-4, the adsorption port 1-4 extends into the inner side of the connection tube 1-2, the back side of the connection tube 1-2 is fixedly connected with a second plugging cover 1-9, the front side of the second plugging cover 1-9 is provided with a rubber ring 1-10, the back side of the second plugging cover 1-9 is provided with a marking mechanism, the A second sealing ring 1-15 is provided on the inner side of the lower end of the connecting head 1-1, a one-way valve 1-14 is provided on the inner side of the connecting pipe 1-2, a threaded groove 1-7 is provided on the upper end of the connecting pipe 1-2, the adsorption column 1-3 is threadedly installed through the connecting part 1-6 and the threaded groove 1-7, a first sealing ring 1-8 is provided on the inner side of the threaded groove 1-7, a peeling block 1-11 is fixedly connected to the upper end of the second blocking cover 1-9, the marking mechanism includes a marking groove, the marking groove is provided on the back side of the second blocking cover 1-9, a soft magnetic layer is adhesively connected to the inner side of the marking groove, an iron sheet is magnetically connected to the outer side of the soft magnetic layer, a label paper is adhesively connected to the outer side of the iron sheet, and an arc groove is provided at the notch of the marking groove.

[0028] This example was applied to the extraction of nucleic acids from corn, rice and wheat. The results of the quality determination of nucleic acids extracted by the vacuum filtration nucleic acid extraction system were as follows: Figure 3 and 4 The OD260 / OD280 of corn, rice, and wheat powder samples were between 1.73 and 1.84, 1.71 and 1.88, and 1.66 and 1.88, respectively, which were in line with the expected range of 1.6 to 2.0. The extracted nucleic acid concentrations in ng / μl were between 62.5 and 83.0, 56.0 and 82.0, and 53.0 and 83.5, respectively, which met the detection requirements.

[0029] The extracted nucleic acid was detected by electrophoresis using 0.8% agarose gel electrophoresis. Figure 5 As shown, the electrophoresis brightness of the extracted nucleic acid of corn, rice and wheat is high, with little dispersion and no tailing, which meets the requirements for the next step of genetically modified component analysis.

[0030] The internal standard genes were amplified using real-time fluorescence PCR to obtain Figure 6-8 As shown in the amplification curves, the internal standard genes zSSIIb, SPS and Wx012 of corn, rice and wheat can be completely amplified, and the Ct values ​​are between 24-26, indicating that the extracted corn, rice and wheat nucleic acids are suitable for PCR detection of genetically modified components.

[0031] Compared with traditional methods, the nucleic acid extraction process has been greatly simplified. Taking the extraction of 10 corn seed samples as an example, the extraction steps were reduced from the original 14 steps to 7 steps, and the time taken was shortened from the original 142 minutes to 58 minutes, which reduced the time required by about 60% and increased the efficiency by more than 140%. Since the non-contact connection device was used in the experiment, the use of consumables was reduced. On average, 40 centrifuge tubes and connectors were reduced for every 10 samples, which reduced the use of disposable experimental consumables and reduced experimental waste, and it has a high use value.

[0032] The above is only a preferred embodiment of the utility model, and does not limit the utility model in any way. Any simple modification, change and equivalent change made to the above embodiment according to the essence of the utility model technology still falls within the protection scope of the technical solution of the utility model.

Claims

1. A vacuum filtration nucleic acid extraction device, characterized in that: The invention comprises a vacuum box (2), a vacuum pump (3) and a non-contact connection cylinder (1); the top of the vacuum box (2) is connected to the non-contact connection cylinder (1) via a plurality of connection holes; a vacuum pump (3) for evacuating the vacuum box (2) is fixedly provided on the vacuum box (2); and a waste liquid outlet (4) is provided at the lower part of the side wall of the vacuum box (2).

2. A vacuum filtration nucleic acid extraction device according to claim 1, characterized in that: The non-contact connection cylinder (1) comprises a connection tube (1-2) and an adsorption column (1-3); the lower end of the connection tube (1-2) is fixedly connected to a connection head (1-1); the outer side of the adsorption column (1-3) is provided with a connection portion (1-6); the adsorption column (1-3) is threadedly connected to the connection tube (1-2) through the connection portion (1-6); the upper end of the adsorption column (1-3) is provided with a first blocking cover (1-5); the lower end of the adsorption column (1-3) is provided with an adsorption port (1-4); the adsorption port (1-4) extends into the inner side of the connection tube (1-2); the back side of the connection tube (1-2) is fixedly connected to a second blocking cover (1-9); the front side of the second blocking cover (1-9) is provided with a rubber ring (1-10); the back side of the second blocking cover (1-9) is provided with a marking machine The connecting head (1-1) is provided with a second sealing ring (1-15) on the inner side of the lower end, the connecting pipe (1-2) is provided with a one-way valve (1-14) on the inner side, the connecting pipe (1-2) is provided with a threaded groove (1-7) on the upper end, the adsorption column (1-3) is threadedly installed through the connecting portion (1-6) and the threaded groove (1-7), the inner side of the threaded groove (1-7) is provided with a first sealing ring (1-8), the upper end of the second blocking cover (1-9) is fixedly connected with a peeling block (1-11), the marking mechanism comprises a marking groove, the marking groove is provided on the back side of the second blocking cover (1-9), the inner side of the marking groove is bonded with a soft magnetic layer, the outer side of the soft magnetic layer is magnetically connected with an iron sheet, the outer side of the iron sheet is bonded with a label paper, and an arc groove is provided at the groove of the marking groove.

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