Electrode device of in-vivo gene electrotransduction instrument

Abstract

The utility model relates to the field of electrode device design, and discloses an electrode device of an in vivo gene electrotransduction instrument, which comprises an upper shell, a sliding block I is connected in the upper shell in a sliding manner, a plurality of sliding blocks IV are uniformly and fixedly connected to the periphery of the sliding block I, a limiting plate is fixedly connected in the upper shell, a limiting block I is fixedly connected to the lower part of the limiting plate, and the limiting block I is fixedly connected to the lower part of the limiting plate. A limiting groove is formed in the first limiting block, a second sliding block is slidably connected to the inner side of the first limiting block, a third sliding block is fixedly connected to the periphery of the second sliding block, the third sliding block is slidably connected to the interior of the limiting groove, and the lower portion of the upper shell abuts against a middle shell. According to the electrode pen, through cooperation of the shell, the spring, the sliding block, the limiting block, the limiting plate, the protection tube and the limiting groove, rapid insertion and extraction of the electrode are achieved, the operation time and labor intensity are reduced, through the installation assembly, the electrode pen is convenient to assemble and disassemble, and the internal electrode is convenient to replace and maintain.

Description

Technical Field

[0001] The utility model relates to the field of electrode device design, in particular to an electrode device of an in vivo gene electrotransfer instrument. Background Art

[0002] With the development of biotechnology, gene transfection technology plays an increasingly important role in biological research. In vivo gene electroporation is a medical device used to introduce exogenous genes into the body through electroporation technology for gene therapy or gene expression research. The device is usually equipped with special electrodes for generating electroporation on the cell membrane, so that the exogenous gene can enter the cell. In vivo gene electroporation has many advantages, such as the ability to introduce substances such as DNA, RNA or protein into cells, easy operation, good repeatability, and high introduction efficiency. The device has a wide range of applications in gene therapy, vaccine development, tumor research and other fields.

[0003] In the application of in vivo gene electrotransfer instrument, the insertion and removal of electrodes are currently mainly completed manually, which is not only laborious, but also increases the operation time and affects the treatment effect. Therefore, an electrode device of in vivo gene electrotransfer instrument is proposed to solve the above problems. Utility Model Content

[0004] In order to make up for the above shortcomings, the utility model provides an electrode device for an in vivo gene electroporator, aiming to improve the problem that the insertion and removal of electrodes in the prior art are currently mainly completed manually, which is not only laborious, but also increases the operation time and affects the treatment effect.

[0005] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: an electrode device of an in vivo gene electrotransfer instrument comprises an upper shell, a slider 1 is slidably connected inside the upper shell, a plurality of sliders 4 are evenly fixedly connected to the outer periphery of the slider 1, a limiting plate is fixedly connected inside the upper shell, a limiting block 1 is fixedly connected to the lower part of the limiting plate, a limiting groove is provided inside the limiting block 1, a slider 2 is slidably connected to the inner side of the limiting block 1, a slider 3 is fixedly connected to the outer periphery of the slider 2, and the slider 3 is slidably connected to the inside of the limiting groove, a middle shell is abutted at the lower part of the upper shell, a lower shell is abutted at the lower part of the middle shell, a protective tube is fixedly connected inside the slider 1, a limiting block 2 is fixedly connected to the outer periphery of the protective tube, a spring is abutted between the limiting block 2 and the lower shell, an electrode tube is fixedly connected inside the protective tube, positive and negative electrodes are respectively fixedly connected to the bottom ends of the electrode tube, and mounting components are provided at both ends of the middle shell.

[0006] Through the above technical solution, the spring used is a warning spring.

[0007] As a further description of the above technical solution:

[0008] The installation assembly includes a threaded connection pipe 1, which is fixedly connected to the upper part of the middle shell, and the threaded connection pipe 1 is threadedly connected to the inside of the upper shell. The lower inner side of the middle shell is threadedly connected to a threaded connection pipe 2, and the threaded connection pipe 2 is fixedly connected to the upper part of the lower shell.

[0009] Through the above technical solution, the shells are connected by threads, which facilitates assembly and disassembly.

[0010] As a further description of the above technical solution:

[0011] An upper cover is threadedly connected to an upper portion of the sliding block, and a cap rod is fixedly connected to the outer periphery of the upper shell.

[0012] Through the above technical solution, the upper cover is used to protect the slider one.

[0013] As a further description of the above technical solution:

[0014] The protection tube is slidably connected to the interior of the lower shell, and the upper cover is slidably connected to the interior of the upper shell.

[0015] Through the above technical solution, the protective tube uses insulating material to prevent electric shock caused by contact with the shell.

[0016] As a further description of the above technical solution:

[0017] The slider three abuts against a lower portion of the slider, and the lower portion of the slider two abuts against a lower portion of the limit block.

[0018] Through the above technical solution, the limit block 1 is used to limit the moving trajectory of the slider 2.

[0019] As a further description of the above technical solution:

[0020] The spring sleeve is arranged on the outer periphery of the protection tube, and a circuit is fixedly connected to the upper part of the electrode tube.

[0021] Through the above technical solution, the spring is used to provide elastic force, and after pressing the slider one, the limit block two is squeezed to bounce the protection tube back into the shell.

[0022] As a further description of the above technical solution:

[0023] The circuit is arranged through the middle part of the upper cover, and the circuit is slidably connected to the middle part of the slider.

[0024] Through the above technical solution, the circuit is used to connect the electrode tube and the power supply.

[0025] As a further description of the above technical solution:

[0026] The protection tube and the middle shell body are not in contact with each other.

[0027] The above technical solution can prevent the electrode tube from contacting the shell and causing leakage.

[0028] The utility model has the following beneficial effects:

[0029] 1. In the utility model, through the cooperation of the upper shell, the lower shell, the middle shell, the spring, the slider 1, the slider 2, the slider 3, the limit block 1, the limit block 2, the slider 4, the limit plate, the protection tube and the limit groove, the spring drives the electrode tube to pop out and retract, and the slider 2 is driven down by pressing the slider 1, so that the slider 3 is in the limit grooves of different lengths, so that the electrode tube is extended and retracted into the shell, and the electrode is quickly inserted and pulled out, which reduces the operation time and labor intensity. At the same time, the device has a simple and compact structure and is easy to carry and use.

[0030] 2. In the utility model, the shells are connected by threads through the installation components, which makes it convenient to assemble and disassemble the electrode pen, replace and maintain the internal electrodes, and install middle shells of different lengths according to the lengths of the electrode tubes. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a three-dimensional schematic diagram of the electrode device of the in vivo gene electrotransfer instrument proposed by the utility model;

[0032] Figure 2 This is a schematic structural diagram of the middle shell of the electrode device of the in vivo gene electrotransfer instrument proposed by the utility model;

[0033] Figure 3 This is a schematic diagram of the structure of the protection tube of the electrode device of the in vivo gene electrotransfer instrument proposed by the utility model;

[0034] Figure 4 This is a schematic structural diagram of a limit plate of an electrode device of an in vivo gene electrotransfer instrument proposed by the utility model;

[0035] Figure 5 for Figure 3 Enlarged view of point A in the middle;

[0036] Figure 6 for Figure 3 Enlarged view of point B in the middle.

[0037] Legend:

[0038] 1. Upper shell; 2. Middle shell; 3. Cap rod; 4. Lower shell; 5. Upper cover; 6. Circuit; 7. Threaded connection tube 1; 8. Threaded connection tube 2; 9. Slider 1; 10. Protection tube; 11. Spring; 12. Electrode tube; 13. Limit block 1; 14. Limit groove; 15. Limit plate; 16. Slider 2; 17. Slider 3; 18. Slider 4; 19. Limit block 2. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0040] Reference Figure 3 , Figure 5 and Figure 6 The utility model provides an embodiment: an electrode device of an in vivo gene electrotransfer instrument, comprising an upper shell 1, a slider 9 is slidably connected inside the upper shell 1, a plurality of sliders 18 are evenly fixedly connected to the outer periphery of the slider 9, a limiting plate 15 is fixedly connected inside the upper shell 1, a limiting block 13 is fixedly connected to the lower part of the limiting plate 15, a limiting groove 14 is provided inside the limiting block 13, a slider 2 is slidably connected to the inner side of the limiting block 13, a slider 3 17 is fixedly connected to the outer periphery of the slider 2 16, and the slider 3 17 is slidably connected inside the limiting groove 14, a middle shell 2 is abutted at the lower part of the upper shell 1, a lower shell 4 is abutted at the lower part of the middle shell 2, a protective tube 10 is fixedly connected inside the slider 9, a limiting block 2 19 is fixedly connected to the outer periphery of the protective tube 10, a spring 11 is abutted between the limiting block 2 19 and the lower shell 4, an electrode tube 12 is fixedly connected inside the protective tube 10, a positive electrode and a negative electrode are fixedly connected to the bottom end of the electrode tube 12, and mounting components are arranged at both ends of the middle shell 2. The protection tube 10 is slidably connected to the interior of the lower shell 4, and the upper cover 5 is slidably connected to the interior of the upper shell 1. The slider 3 17 abuts against the lower part of the slider 1 9, and the lower part of the slider 2 16 abuts against the lower part of the limit block 1 13. The spring 11 is sleeved on the outer periphery of the protection tube 10, and the upper part of the electrode tube 12 is fixedly connected with the circuit 6.

[0041] Specifically, the components of the electrode device include an upper shell 1, which serves as the outer shell of the entire electrode device and plays a structural support and protection role for the internal components. A slider 9 is provided inside the upper shell 1, and the slider 9 interacts with the upper shell 1 in a sliding connection. A plurality of sliders 18 are evenly and fixedly connected to the outer periphery of the slider 9, and these sliders 18 are connected to the slider 9 to achieve smooth sliding and adjust the position of the internal components. A limit plate 15 is fixedly connected to the inner part of the upper shell 1, and is used to set the moving range of the slider 9. A limit block 13 is provided at the bottom of the limit plate 15, and the vertical movement of the slider 9 is limited by the limit groove 14 inside the limit block 13. The slider 2 16 is located on the inner side of the limit block 13 and is connected to it by a sliding connection. A slider 3 17 is connected to the outer periphery of the slider 2 16, and the slider 3 17 is connected to the slider 2 16, and a sliding connection is achieved inside the limit groove 14. The middle shell 2 is located at the lower part of the upper shell 1, providing additional support and structure for the electrode device. The upper part of the middle shell 2 is connected to the upper shell 1, and the lower part abuts against the lower shell 4. The electrode tube 12 is fixedly connected inside the protection tube 10, and is connected to the lower shell 4 through the second limit block 19. The spring 11 is sleeved on the outer periphery of the protection tube 10, connected between the second limit block 19 and the lower shell 4, and provides buffering and damping functions for the electrode device.

[0042] Reference Figure 1 , Figure 2 and Figure 4 The installation assembly includes a threaded connection pipe 7, which is fixedly connected to the upper part of the middle shell 2, and the threaded connection pipe 7 is threadedly connected to the inside of the upper shell 1. The lower inner side of the middle shell 2 is threadedly connected with a threaded connection pipe 2 8, and the threaded connection pipe 2 8 is fixedly connected to the upper part of the lower shell 4.

[0043] Specifically, the mounting assembly includes a threaded connection pipe 1 7 and a threaded connection pipe 2 8, which form a connection between the middle shell 2 and the upper shell 1 and the lower shell 4 to provide necessary structural support. The shells are connected by threads to achieve convenient disassembly and assembly.

[0044] Reference Figure 1-Figure 3 The upper part of the slider 9 is threadedly connected with the upper cover 5, and the outer periphery of the upper shell 1 is fixedly connected with the cap rod 3. The line 6 is arranged in the middle of the upper cover 5, and the line 6 is slidably connected to the middle of the slider 9. The protection tube 10 and the middle shell 2 do not contact each other.

[0045] Specifically, the upper cover 5 is connected to the inner shell by sliding, and a threaded interface is provided on the upper part thereof for fixing the slider 3 17. The circuit 6 runs through the middle of the upper cover 5 and is slidably connected with the middle of the slider 1 9 and the slider 3 17. A circuit is fixedly provided on the upper part of the electrode tube 12, and is connected with the circuit of the gene electrotransfer instrument.

[0046] Working principle: Press the upper cover 5 to make the slider 1 9 drop, thereby driving the slider 2 16 to move, so that the slider 3 17 is in different limit slots 14. The limit slots 14 are of different lengths. In the long limit slot 14, the electrode is in a retracted state, and in the short limit slot 14, the electrode is in an extended state. The spring 11 squeezes the limit block 2 19 so that the protection tube 10 drives the electrode tube 12 to move, thereby performing the ejection and retraction of the electrode tube 12.

[0047] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An electrode device for an in vivo gene electrotransfer instrument, comprising an upper housing (1), characterized in that: The upper shell (1) is slidably connected to a slider 1 (9), and a plurality of sliders 4 (18) are evenly fixedly connected to the outer periphery of the slider 1 (9). The upper shell (1) is fixedly connected to a limit plate (15), and a limit block 1 (13) is fixedly connected to the lower part of the limit plate (15). A limit groove (14) is provided inside the limit block 1 (13). A slider 2 (16) is slidably connected to the inner side of the limit block 1 (13), and a slider 3 (17) is fixedly connected to the outer periphery of the slider 2 (16). The slider 3 (17) is slidably connected to the limit groove (1 4), the lower part of the upper shell (1) abuts against the middle shell (2), the lower part of the middle shell (2) abuts against the lower shell (4), the slider (9) is fixedly connected to a protection tube (10), the outer periphery of the protection tube (10) is fixedly connected to a limit block (19), a spring (11) abuts between the limit block (19) and the lower shell (4), the protection tube (10) is fixedly connected to an electrode tube (12), the bottom end of the electrode tube (12) is respectively fixedly connected to positive and negative electrodes, and mounting components are provided at both ends of the middle shell (2).

2. The electrode device for in vivo gene electroporation according to claim 1, characterized in that: The mounting assembly comprises a threaded connection pipe (7), the threaded connection pipe (7) being fixedly connected to the upper part of the middle shell (2), the threaded connection pipe (7) being threadedly connected to the interior of the upper shell (1), the inner lower part of the middle shell (2) being threadedly connected to a threaded connection pipe (8), the threaded connection pipe (8) being fixedly connected to the upper part of the lower shell (4).

3. The electrode device for in vivo gene electroporation according to claim 2, characterized in that: The upper portion of the slider (9) is threadedly connected to an upper cover (5), and the outer periphery of the upper shell (1) is fixedly connected to a cap rod (3).

4. The electrode device for in vivo gene electroporation according to claim 3, characterized in that: The protection tube (10) is slidably connected inside the lower shell (4), and the upper cover (5) is slidably connected inside the upper shell (1).

5. The electrode device for in vivo gene electroporation according to claim 4, characterized in that: The slider three (17) abuts against the lower part of the slider one (9), and the lower part of the slider two (16) abuts against the lower part of the limit block one (13).

6. The electrode device for in vivo gene electroporation according to claim 3, characterized in that: The spring (11) is sleeved on the outer circumference of the protection tube (10), and a circuit (6) is fixedly connected to the upper part of the electrode tube (12).

7. The electrode device for in vivo gene electroporation according to claim 6, characterized in that: The circuit (6) is arranged through the middle of the upper cover (5), and the circuit (6) is slidably connected to the middle of the slider 1 (9).

8. The electrode device for in vivo gene electroporation according to claim 1, characterized in that: The protection tube (10) and the middle shell (2) are not in contact with each other.

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