Nucleic acid and protein extraction equipment

Abstract

The utility model provides nucleic acid and protein extraction equipment, which belongs to the technical field of nucleic acid and protein extraction, and comprises an extraction cylinder and mounting openings annularly and uniformly arranged on the lower end surface of the extraction cylinder, discharge pipes are arranged in the mounting openings, electromagnetic valves are connected in series in the discharge pipes, and an extraction crushing mechanism is arranged in the extraction cylinder. The extracting and smashing mechanism comprises a filtering funnel movably placed in the extracting cylinder, a rotating rod is rotationally connected to the middle of the lower end of the filtering funnel, a spiral piece is arranged on the outer arc face of the rotating rod, cutting knives are evenly distributed on the outer arc face of the rotating rod, and a turning assembly is further arranged at the bottom of the extracting cylinder. By means of the extracting and smashing mechanism, plants are smashed more thoroughly, the utilization efficiency of plant raw materials is further improved, nucleic acid and protein can be extracted more easily and can be put into the next extraction operation after being rapidly disassembled, assembled and cleaned, and the problem that ineffective working hours are increased due to inconvenient cleaning is solved.

Description

Technical Field

[0001] The utility model belongs to the technical field of nucleic acid and protein extraction, and in particular relates to nucleic acid and protein extraction equipment. Background Art

[0002] Nucleic acids and proteins are the material basis of life, organic macromolecules, and the basic organic matter that constitutes cells. At present, there are quite a lot of nucleic acid and protein products on the market that are made from plants as raw materials. Because plants contain very rich plant proteins, which can increase the nutritional elements of products, they are widely used in various foods, feeds, medicinal materials and nutritional products.

[0003] When the existing nucleic acid and protein extraction equipment is in use, the cutting blade is set to cut and crush the incoming raw materials, and then cooperate with the setting of the internal filtering structure of the device to facilitate the subsequent protein precipitation and extraction work. Although the extraction of nucleic acids and proteins can be achieved, there are still some problems in use. The plant raw materials are not crushed thoroughly enough, which leads to low utilization efficiency and extraction efficiency of the plant raw materials, resulting in a large amount of nucleic acid and protein waste. Secondly, most of the existing equipment is an integrated design, which is more troublesome to clean and is not convenient for quick use in the next extraction operation. The inconvenient cleaning will lead to an increase in invalid working hours. Utility Model Content

[0004] In view of this, the utility model provides a nucleic acid and protein extraction device, which can make the plant crushing more thoroughly through the extraction and crushing mechanism, thereby improving the utilization efficiency of the plant raw materials, making the raw material mixed solution and the raw materials constantly surge, thereby further improving the crushing effect of the plant raw materials, so that the nucleic acid and protein in the plant can be fully mixed with the solution used for its extraction, which can make the extraction of nucleic acid and protein easier, and the split design can be quickly disassembled and cleaned and put into the next extraction operation, which solves the problem of increased invalid working hours due to inconvenient cleaning and improves the work efficiency of the staff.

[0005] In order to solve the above technical problems, the utility model provides a nucleic acid and protein extraction device, including an extraction cylinder and an installation opening annularly and evenly arranged on the lower end surface of the extraction cylinder, a discharge pipe is arranged in the installation opening, a solenoid valve is connected in series in the discharge pipe, an extraction crushing mechanism is arranged in the extraction cylinder, the extraction crushing mechanism includes a filter funnel movably placed in the extraction cylinder, a rotating rod is rotatably connected to the middle part of the lower end of the filter funnel, a spiral sheet is arranged on the outer arc surface of the rotating rod, and evenly distributed cutting knives are arranged on the outer arc surface of the rotating rod, a tumbling component is also arranged at the bottom of the extraction cylinder, a limit component for limiting the filter funnel is also arranged at the upper end of the extraction cylinder, a storage component is also arranged at the lower end of the extraction cylinder, and the solenoid valves are electrically connected to an external single-chip computer.

[0006] The limiting assembly includes a limiting seat evenly arranged in an annular shape on the upper end of the outer arc surface of the extraction cylinder, the top wall of the filter funnel is provided with limiting rods evenly distributed in an annular shape, and the middle part of the limiting seat is provided with limiting jacks corresponding to the limiting rods one by one.

[0007] A ring-shaped evenly distributed mounting plate is arranged in the middle of the outer arc surface of the extraction cylinder.

[0008] The storage assembly comprises a threaded ring arranged at the lower end of the extraction cylinder, and the external thread of the threaded ring is connected with a storage barrel.

[0009] The extraction and crushing mechanism also includes a motor arranged in the middle of the bottom wall of the extraction cylinder, a rectangular connector is provided at the upper end of the output shaft of the motor, a rectangular socket matched with the rectangular connector is provided at the lower end surface of the rotating rod, and the motor is electrically connected to an external single-chip computer; the extraction and crushing mechanism also includes a protective cover arranged in the middle of the bottom wall of the extraction cylinder, and a through hole matching with the output shaft of the motor is provided in the middle of the upper end of the protective cover.

[0010] The tumbling assembly includes a connecting pipe arranged in the middle of the bottom wall of the extraction cylinder. The air inlet of the connecting pipe passes through the outer arc surface of the extraction cylinder and extends to the outside. One-way air outlet pipes are provided in the air outlets evenly arranged in a ring at the upper end of the outer arc surface of the connecting pipe. The one-way air outlet pipes pass through the protective cover and extend to the inside of the extraction cylinder.

[0011] The beneficial effects of the above technical solution of the utility model are as follows:

[0012] 1. The relevant staff injects an appropriate amount of solution for extracting nucleic acids and proteins into the extraction cylinder, and then places the plants to be extracted with nucleic acids and proteins in the filter funnel. The relevant staff then controls the operation of the motor through an external single-chip microcomputer. The rotation of the motor output shaft drives the rectangular connector, the rotating rod, the spiral blade and the cutting knife to rotate synchronously. The cutting knife crushes and cuts the plants in the filter funnel, and the spiral blade continuously flips the plants in the filter funnel upward, so that the plants are crushed more thoroughly, thereby improving the utilization efficiency of plant raw materials.

[0013] 2. At the same time, the connecting tube is connected to the external air pump, and the external air pump operates through the connecting tube and the one-way air outlet pipe, so that the raw material mixed solution and the raw material are constantly surging, thereby further improving the crushing effect of the plant raw material, so that the nucleic acid and protein in the plant can be fully mixed with the solution used for its extraction, which can make the extraction of nucleic acid and protein easier.

[0014] 3. When the stirring and crushing time reaches the set value, the external single-chip microcomputer controls the solenoid valve to open. At this time, the raw material mixture enters the storage barrel through the discharge pipe, and the raw material particles remain in the filter funnel. At this time, the raw material mixture in the storage barrel is extracted to achieve nucleic acid and protein extraction. Finally, you only need to unscrew the storage barrel counterclockwise from the threaded ring. The operation is relatively convenient and quick.

[0015] 4. When the extraction is completed, just lift the filter funnel upwards, then the rotating rod will be separated from the rectangular connector, and then the filter funnel can be turned upside down to clean the raw material residue, and then the filter funnel and the extraction cylinder can be cleaned. Through the split design, it can be quickly disassembled and cleaned and put into the next extraction operation, which solves the problem of increased invalid working hours due to inconvenient cleaning and improves the work efficiency of the staff. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the main structure of a nucleic acid and protein extraction device of the utility model;

[0017] Figure 2 This is a schematic diagram of the structure of the extraction and crushing mechanism of the utility model;

[0018] Figure 3 It is a schematic diagram of the enlarged structure of point A of the utility model;

[0019] Figure 4 It is a schematic diagram of the enlarged structure of point B of the utility model.

[0020] Explanation of the reference numerals: 100, extraction cylinder; 200, discharge pipe; 300, solenoid valve; 400, filter funnel; 401, rotating rod; 402, spiral blade; 403, cutting knife; 404, motor; 405, rectangular connector; 406, protective cover; 500, connecting pipe; 501, one-way air outlet pipe; 600, limit seat; 601, limit rod; 700, threaded ring; 701, storage barrel; 800, mounting plate. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical scheme and advantages of the embodiment of the utility model clearer, the following will be combined with the appended drawings of the embodiment of the utility model. Figure 1-4 , the technical scheme of the embodiment of the utility model is clearly and completely described. Obviously, the described embodiment is a part of the embodiment of the utility model, not all of the embodiments. Based on the described embodiment of the utility model, all other embodiments obtained by ordinary technicians in this field belong to the scope of protection of the utility model.

[0022] like Figure 1-4 As shown:

[0023] The present embodiment provides a nucleic acid and protein extraction device, including an extraction cylinder 100 and an installation opening evenly arranged in an annular manner on the lower end surface of the extraction cylinder 100, wherein a discharge pipe 200 is arranged in the installation opening, and a solenoid valve 300 is connected in series in the discharge pipe 200, and an extraction crushing mechanism is arranged in the extraction cylinder 100, wherein the extraction crushing mechanism comprises a filter funnel 400 movably placed in the extraction cylinder 100, wherein a rotating rod 401 is rotatably connected to the middle part of the lower end of the filter funnel 400, wherein a spiral sheet 402 is arranged on the outer arc surface of the rotating rod 401, and evenly distributed cutting knives 403 are arranged on the outer arc surface of the rotating rod 401, and a tumbling component is also arranged at the bottom of the extraction cylinder 100, wherein the extraction cylinder 100 is provided with a plurality of screw blades 402, wherein the outer arc surface of the rotating rod 401 is provided with uniformly distributed cutting knives 403 ... 0 is also provided with a limit assembly for limiting the filter funnel 400, and a storage assembly is also provided at the lower end of the extraction cylinder 100. The solenoid valve 300 is electrically connected to the external single-chip computer; the extraction and crushing mechanism also includes a motor 404 arranged in the middle of the bottom wall of the extraction cylinder 100, and a rectangular connector 405 is provided at the upper end of the output shaft of the motor 404. A rectangular jack matched with the rectangular connector 405 is provided on the lower end surface of the rotating rod 401, and the motor 404 is electrically connected to the external single-chip computer; the extraction and crushing mechanism also includes a protective cover 406 arranged in the middle of the bottom wall of the extraction cylinder 100, and a through hole matching the output shaft of the motor 404 is provided in the middle of the upper end of the protective cover 406.

[0024] like Figure 2-4 As shown, the surging component includes a connecting pipe 500 arranged in the middle of the bottom wall of the extraction cylinder 100, the air inlet of the connecting pipe 500 passes through the outer arc surface of the extraction cylinder 100 and extends to the outside, and the air outlets evenly arranged in a ring at the upper end of the outer arc surface of the connecting pipe 500 are provided with one-way air outlet pipes 501, and the one-way air outlet pipes 501 pass through the protective cover 406 and extend to the inside of the extraction cylinder 100.

[0025] like Figure 1-3 As shown, the limiting assembly includes a limiting seat 600 which is evenly arranged in an annular shape on the upper end of the outer arc surface of the extraction cylinder 100, the top wall surface of the filter funnel 400 is provided with limiting rods 601 which are evenly distributed in an annular shape, and the middle part of the limiting seat 600 is provided with limiting sockets which correspond one to one with the limiting rods 601.

[0026] like Figure 1-4 As shown, the storage assembly includes a threaded ring 700 disposed at the lower end of the extraction cylinder 100 , and the external thread of the threaded ring 700 is connected to a storage barrel 701 .

[0027] The working principle of a nucleic acid and protein extraction device provided by the utility model is as follows: first, the relevant staff injects an appropriate amount of solution for extracting nucleic acids and proteins into the extraction cylinder 100, and then places the plant to be extracted with nucleic acids and proteins in the filter funnel 400, and then the relevant staff controls the operation of the motor 404 through an external single-chip microcomputer, and the output shaft of the motor 404 rotates to drive the rectangular connector 405, the rotating rod 401, the spiral piece 402 and the cutting knife 403 to rotate synchronously, and the cutting knife 403 crushes and cuts the plants in the filter funnel 400, and the spiral piece 402 will continuously flip the plants in the filter funnel 400 upwards, so that the plants are crushed more thoroughly, thereby improving the utilization efficiency of the plant raw materials, and at the same time, the connecting pipe 500 is connected to the external air pump, and the operation of the external air pump cooperates with the connecting pipe 500 and the one-way air outlet pipe 501, thereby further improving the crushing effect of the plant raw materials, so that the raw material mixed solution and the raw materials are continuously surging, so that the nucleic acid and The protein is fully mixed with the solution used for its extraction, which can make the extraction of nucleic acid and protein easier. When the stirring and crushing time reaches the set value, the external single-chip microcomputer controls the solenoid valve 300 to open. At this time, the raw material mixture enters the storage barrel 701 through the discharge pipe 200, and the raw material particles remain in the filter funnel 400. At this time, the raw material mixture in the storage barrel 701 is extracted to achieve nucleic acid and protein extraction. Finally, it is only necessary to unscrew the storage barrel 701 from the threaded ring 700 counterclockwise. The operation is relatively convenient and fast. When the extraction is completed, it is only necessary to lift the filter funnel 400 upward. At this time, the rotating rod 401 is separated from the rectangular connector 405, and then the filter funnel 400 is turned upside down to clean the raw material residue, and then the filter funnel 400 and the extraction cylinder 100 are cleaned. Through the split design, it can be quickly disassembled and cleaned and put into the next extraction operation, which solves the problem of increased invalid working hours due to inconvenient cleaning and improves the work efficiency of the staff.

[0028] like Figure 1-2 As shown, a ring-shaped evenly distributed mounting plate 800 is provided in the middle of the outer arc surface of the extraction cylinder 100 to facilitate the relevant staff to fix and install the extraction cylinder 100.

[0029] In addition, it should be noted that in the description of the present invention, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to the specific circumstances.

[0030] The above is a preferred embodiment of the present invention. It should be pointed out that, for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A nucleic acid and protein extraction device, characterized in that: The invention comprises an extraction cylinder (100) and an installation opening evenly arranged in an annular shape on the lower end surface of the extraction cylinder (100), wherein a discharge pipe (200) is arranged in each installation opening, wherein a solenoid valve (300) is connected in series in each discharge pipe (200), wherein an extraction and crushing mechanism is arranged in the extraction cylinder (100), wherein the extraction and crushing mechanism comprises a filter funnel (400) movably placed in the extraction cylinder (100), wherein a rotating rod (400) is rotatably connected to the middle part of the lower end of the filter funnel (400) 01), the outer arc surface of the rotating rod (401) is provided with a spiral sheet (402), the outer arc surface of the rotating rod (401) is provided with evenly distributed cutting knives (403), the bottom of the extraction cylinder (100) is also provided with a tumbling component, the upper end of the extraction cylinder (100) is also provided with a limiting component for limiting the filter funnel (400), the lower end of the extraction cylinder (100) is also provided with a storage component, and the solenoid valve (300) is electrically connected to an external single-chip computer.

2. A nucleic acid and protein extraction device as claimed in claim 1, characterized in that: The extraction and crushing mechanism also includes a motor (404) arranged in the middle of the bottom wall of the extraction cylinder (100), the upper end of the output shaft of the motor (404) is provided with a rectangular connector (405), the lower end surface of the rotating rod (401) is provided with a rectangular socket matched with the rectangular connector (405), and the motor (404) is electrically connected to an external single-chip computer.

3. A nucleic acid and protein extraction device as claimed in claim 2, characterized in that: The extraction and pulverization mechanism further comprises a protective cover (406) arranged in the middle of the bottom wall of the extraction cylinder (100), and a through hole matching the output shaft of the motor (404) is provided in the middle of the upper end of the protective cover (406).

4. A nucleic acid and protein extraction device as claimed in claim 3, characterized in that: The tumbling assembly comprises a connecting pipe (500) arranged in the middle of the bottom wall of the extraction cylinder (100), the air inlet of the connecting pipe (500) penetrates the outer arc surface of the extraction cylinder (100) and extends to the outside, and the air outlets evenly arranged in an annular shape at the upper end of the outer arc surface of the connecting pipe (500) are all provided with one-way air outlet pipes (501), and the one-way air outlet pipes (501) all penetrate the protective cover (406) and extend to the inside of the extraction cylinder (100).

5. A nucleic acid and protein extraction device as claimed in claim 1, characterized in that: The limiting assembly comprises a limiting seat (600) which is evenly arranged in an annular shape on the upper end of the outer arc surface of the extraction cylinder (100); the top wall surface of the filtering funnel (400) is provided with limiting rods (601) evenly distributed in an annular shape; and the middle part of the limiting seat (600) is provided with limiting sockets which correspond one to one with the limiting rods (601).

6. A nucleic acid and protein extraction device as claimed in claim 1, characterized in that: The storage assembly comprises a threaded ring (700) arranged at the lower end of the extraction cylinder (100), and the outer thread of the threaded ring (700) is connected to a storage barrel (701).

7. A nucleic acid and protein extraction device as claimed in claim 1, characterized in that: A ring-shaped evenly distributed mounting plate (800) is provided in the middle of the outer arc surface of the extraction cylinder (100).

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