Virus detector

Abstract

The utility model relates to a virus detector. The virus detector is used for carrying out virus detection on a sample in a detection box, and comprises a box body, an operation panel, a pipetting piece, a magnetic attraction piece and a heating piece, the box body is provided with a first station, a second station located above the first station and a third station located on the side of the first station. The operation board is used for receiving the detection box and is rotatably arranged at the first station, so that any cavity of the detection box can rotate to the position below the second station or the side of the third station; the pipetting piece is arranged at the second station in a manner of moving up and down, and the pipetting piece can load the operation tools in the cavities or unload the operation tools into the corresponding cavities; the magnetic attraction piece is arranged at the third station in a sliding mode and can be close to or away from the cavity used for magnetic bead attraction so that the magnetic beads in the cavity used for magnetic bead attraction can be attracted or separated; the heating piece is arranged on the operation board and can heat the cavity for amplification reaction and the cavity for magnetic bead adsorption.

Description

Technical Field

[0001] The present application relates to the technical field of test kits, and in particular to a virus detector. Background Art

[0002] In the aquaculture industry, viral infections can have a serious impact on the health of farmed products and farming operations, so timely and accurate virus detection is crucial. Currently, when testing samples for viruses, nucleic acids must first be extracted from the samples to be tested, then the extracted nucleic acids must be amplified and tested, and finally the amplification results must be analyzed to determine whether the virus is present in the sample. However, the above virus detection process requires the use of multiple instruments, which not only increases the workload of operators, but also prolongs the detection time. Summary of the invention

[0003] Based on this, it is necessary to provide a virus detector to address the above technical issues.

[0004] A virus detector is used to perform virus detection on a sample in a detection box, wherein the detection box has a plurality of chambers arranged along its circumference, and the virus detector comprises:

[0005] A box body, wherein the box body has a first workstation, a second workstation located above the first workstation, and a third workstation located to the side of the first workstation;

[0006] An operating disk, the operating disk is used to receive the detection box, and the operating disk is rotatably disposed at the first station, so that any of the chambers of the detection box can be rotated to the bottom of the second station or to the side of the third station;

[0007] a liquid transfer member, which is disposed at the second station so as to be movable up and down, and is capable of loading the operating tool in the chamber or unloading the operating tool into the corresponding chamber;

[0008] A magnetic attraction member, which is slidably disposed at the third station and is capable of approaching the chamber for magnetic bead adsorption, so as to adsorb and separate the magnetic beads in the chamber for magnetic bead adsorption; and

[0009] A heating element is disposed on the operation disk and is capable of heating the chamber used for amplification reaction and the chamber used for magnetic bead adsorption.

[0010] In one embodiment, a pipette tip and a retractable push handle are provided at one end of the pipette close to the operating disk;

[0011] The pipette tip can be inserted into the operating tool in an interference fit manner during the descending process to load the operating tool;

[0012] The pusher can push the operating tool toward the operating disk until the operating tool falls off the pipette tip to unload the operating tool.

[0013] In one embodiment, a supporting platform is convexly provided on a side of the operation disk facing the liquid transfer member, and the supporting platform has a notch along the circumference of the operation disk;

[0014] The heating element comprises a resistance wire and a heat-conducting seat, the resistance wire is connected to the heat-conducting seat, the heat-conducting seat is arranged on the operation panel and accommodated in the notch, and the heat-conducting seat and the supporting platform are used to receive the detection box.

[0015] In one embodiment, the heating element further comprises a heat insulating pad, and the heat insulating pad is arranged between the bottom of the heat conductive seat and the top of the operating panel; and / or,

[0016] The heat-conducting seat has a heat-insulating interval with the supporting platform along the circumferential direction of the operating panel.

[0017] In one embodiment, the box body includes a box body body and a mounting seat disposed in the box body body;

[0018] The mounting base includes a chassis and a support frame. The chassis is arranged at the bottom of the box and is used for mounting the operating panel. The support frame is used for mounting the liquid transfer component and has a first window for the magnetic suction component to pass through.

[0019] In one of the embodiments, the box body further includes a shielding shell and an openable and closable protective door;

[0020] The shielding shell cover is mounted on the box body and is provided with a detection chamber protruding toward the inside of the box body, the top of the detection chamber is provided with a second window for the pipetting component to pass through, the bottom of the detection chamber is provided with a third window for exposing the operating panel, and the side of the detection chamber is provided with an operating port communicating with the outside of the box body; the protective door is provided at the operating port.

[0021] In one embodiment, the virus detector further includes a first driving member, and the first driving member is used to drive the operating disk to rotate;

[0022] The first driving member includes a first power member arranged on the box body, a rotating shaft rotatably arranged on the box body, a driving wheel arranged on the output shaft of the first power member, a driven wheel arranged on the rotating shaft, and a conveyor belt tensioned between the driving wheel and the driven wheel, and the rotating shaft is connected to the operating panel.

[0023] In one embodiment, the virus detector further includes a second driving member, and the second driving member is used to drive the pipetting member to move up and down;

[0024] The second driving member includes a second power member arranged on the box body, a screw supported on the box body and connected to the output shaft of the second power member, a screw nut arranged on the screw, and a first connecting member connected to the screw nut and the pipetting member.

[0025] In one embodiment, the virus detector further includes a third driving member, and the third driving member is used to drive the magnetic attraction member to move;

[0026] The third driving member includes a power cylinder arranged on the box body and a second connecting member connected to the piston rod of the power cylinder, and the second connecting member is also connected to the magnetic attraction member.

[0027] In one of the embodiments, a plurality of buttons are provided on the outer side of the box;

[0028] The virus detector also includes a controller disposed in the housing, and the controller adjusts the working state of the operating disk, the pipetting component, the magnetic component or the heating component after detecting that the button is triggered.

[0029] The above-mentioned virus detector integrates functions such as sample homogenization, nucleic acid lysis, washing, elution and amplification detection through the cooperation of the operating panel and the pipetting part, the magnetic suction part and the heating part. Then, after the test box is placed in, the detector can perform a series of operations such as sample homogenization, nucleic acid lysis, washing, elution and amplification detection on the sample in the test box. This can avoid manual transportation of the test box between different devices and reduce labor intensity, and can also continuously perform virus detection on the sample in the test box and shorten the detection time. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 A schematic diagram of the three-dimensional structure of a virus detector provided in one embodiment of the present application when the protective door is closed.

[0031] Figure 2 This is a schematic diagram of the three-dimensional structure of the virus detector provided in one embodiment of the present application when the protective door is separated.

[0032] Figure 3 and Figure 4 Schematic diagram of the three-dimensional structure of the detection box provided in one embodiment of the present application viewed from different angles.

[0033] Figure 5 An exploded diagram of a virus detector provided in accordance with an embodiment of the present application.

[0034] Figure 6 and Figure 7 A schematic diagram of the installation of an operating disk, a pipetting component, a magnetic component and a heating component on a mounting base provided in one embodiment of the present application.

[0035] Figure 8 A schematic diagram of the installation of a heating element on an operating panel provided in one embodiment of the present application.

[0036] Fig. 9 An exploded schematic diagram of a heating element and an operating disk provided in one embodiment of the present application.

[0037] The reference numerals in the accompanying drawings are described as follows:

[0038] 10. Virus detector; 100. Box; 110. Box body; 120. Mounting seat; 121. Chassis; 122. Support frame; 122a. First window; 122b. Fourth window; 123. Tripod; 130. Shielding shell; 131. Detection chamber; 131a. Third window; 131b. Operation port; 140. Protection door; 150. Button; 160. Warning light; 170. Bracket; 200. Operation panel; 210. Carrying platform; 210a. Notch; 210b. First limiting cavity; 300. Liquid transfer element; 310. Liquid transfer gun tip; 400. Magnetic element; 500. Heating element; 510. Resistance wire; 520. Heat transfer seat; 520a. Second limiting cavity ; 520b, opening; 530, thermal insulation pad; 600, first driving member; 610, first power member; 620, conveyor belt; 700, second driving member; 710, second power member; 720, lead screw; 730, lead screw nut; 740, first connecting member; 741, first connecting part; 742, adapter; 7421, detection sheet; 743, second connecting part; 751, first guide rail; 752, first slider; 761, support bearing; 762, limit bearing; 770, position sensor; 800, third power member; 810, power cylinder; 820, second connecting member; 821, third connecting part; 822, fourth connecting part 900, controller; 20, detection box. DETAILED DESCRIPTION

[0039] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present application, so the present application is not limited by the specific embodiments disclosed below.

[0040] In the description of the present application, it should be understood that if the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. appear, the orientation or position relationship indicated by these terms is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application.

[0041] In addition, if the terms "first" or "second" appear, these terms are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of this application, if the term "plurality" appears, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0042] In this application, unless otherwise clearly specified and limited, if the terms "installed", "connected", "connected", "fixed" and the like appear, these terms should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to the specific circumstances.

[0043] In the present application, unless otherwise clearly specified and limited, if there is a description that a first feature is "above" or "below" a second feature, etc., or similar descriptions appear, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "above" and "above" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. The first feature being "below", "below" and "below" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0044] It should be noted that if an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be a central element. If an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. If any, the terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in this application are for illustrative purposes only and do not represent the only implementation method.

[0045] like Figure 1 and Figure 2 As shown, an embodiment of the present application provides a virus detector 10, which is used to Figure 3 and Figure 4 The sample in the test box 20 shown is tested for viruses.

[0046] The detection box 20 has a plurality of chambers arranged along its circumference. The number of chambers can be set accordingly according to the needs, as long as the virus detection of the sample can be guaranteed. For example, Figure 3 As shown, the detection box 20 is provided with 10 chambers, namely, the sample chamber S, the first tool chamber T1, the second tool chamber T2, the first washing liquid chamber C, the second washing liquid chamber D, the lysing liquid chamber B, the eluting liquid chamber E, the freeze-dried ball chamber T3, the magnetic bead adsorption chamber A and the detection reaction chamber F. Among them, the sample chamber S is pre-filled with a homogenate, the first tool chamber T1 is equipped with a grinding rod L, the side wall of the grinding rod L has a filter grid, the second tool chamber T2 is equipped with a pipette tip (for example, a Tip pipette tip with a capacity of 1000 μL), the first washing liquid chamber C is equipped with a washing liquid 1 (for example, Buffer RWA 01), the second washing liquid chamber D is equipped with a washing liquid 2 (for example, Buffer RWB 01), the lysing liquid chamber B is equipped with a lysing liquid (for example, Buffer RL01), the eluting liquid chamber E is equipped with an eluting liquid (for example, Buffer RE 01), the freeze-dried ball chamber T3 is equipped with freeze-dried balls, the magnetic bead adsorption chamber A is equipped with magnetic beads, and the bottom of the detection reaction chamber F is equipped with a test paper strip.

[0047] See also Figure 2 and Figure 5In some embodiments, the virus detector 10 includes: a housing 100, an operating disk 200, a liquid transfer component 300, a magnetic component 400 and a heating component 500; the housing 100 has a first station, a second station located above the first station and a third station located to the side of the first station; the operating disk 200 is used to support the detection box 20, and the operating disk 200 is rotatably arranged at the first station so that any chamber of the detection box 20 can be rotated to the bottom of the second station or to the side of the third station; the liquid transfer component 300 can load the operating tools in the chamber of the detection box 20 (for example, the grinding rod L in the first tool chamber T1 and the Tip suction head in the second tool chamber T2) or unload the operating tools into the corresponding chamber; the magnetic suction component 400 can be slidably arranged at the third station, and can approach or move away from the chamber for magnetic bead adsorption to adsorb and separate the magnetic beads in the chamber for magnetic bead adsorption; the heating component 500 is arranged on the operating disk 200, and can heat the chamber for amplification reaction and the chamber for magnetic bead adsorption.

[0048] Below Figure 3 and Figure 4 Taking the detection box 20 shown as an example, the working process of the virus detector 10 is described:

[0049] (1) Loading the sample onto the machine

[0050] First, tear off the sealing film of the detection box 20, take the shrimp seedlings and other samples into the sample cavity S; (if and then, push the detection box 20 with the sample into the virus detector 10, and the virus detector 10 starts to run automatically. It should be noted that if the detector is used in conjunction with a PCR (Polymerase chain reaction) instrument, such as Figure 2 As shown, the detection box 20 is further provided with a third tool cavity T4, and the third tool cavity T4 is provided with a PCR tube.

[0051] (2) Sample grinding

[0052] First, the first tool cavity T1 is rotated to the bottom of the pipette 300, and the pipette 300 is loaded with the grinding rod L in the first tool cavity T1; then the sample cavity S is rotated to the bottom of the pipette 300, and the pipette 300 moves the grinding rod L up and down in the sample cavity S to fully grind the sample.

[0053] Then, the pipette 300 is moved downward to lower the grinding rod L to the bottom of the sample chamber S. After waiting for several seconds (eg, 10 seconds), the sample slurry enters the hollow section of the grinding rod after being coarsely filtered through the filter grid. The pipette 300 unloads the grinding rod L in situ.

[0054] Then, rotate the second tool cavity T2 to the bottom of the pipette 300, and load the pipette 300 with 1000μL of Tip tip in the second tool cavity T2; then, rotate the sample cavity S to the bottom of the pipette 300, move the pipette 300 downward, and lower the Tip tip to the bottom of the hollow section of the grinding rod L to absorb 100μL of sample liquid; then, rotate the magnetic bead adsorption cavity A to the bottom of the pipette 300, and release the sample liquid in the Tip tip into the magnetic bead adsorption cavity A.

[0055] (3) Cleavage and Binding

[0056] First, rotate the lysis liquid chamber B to the bottom of the pipette 300, move the pipette 300 downward, and lower the Tip pipette into the lysis liquid chamber B to absorb 500 μL of Buffer RL 01; then, rotate the magnetic bead adsorption chamber A to the bottom of the pipette 300, release the sample liquid in the Tip pipette into the magnetic bead adsorption chamber A, and use the pipette 300 to blow and mix for several minutes (for example, 3 minutes).

[0057] After the oscillation stops, the magnetic suction component 400 is brought close to the magnetic bead adsorption chamber A to attract the magnet for several seconds (for example, 20 seconds) until the magnetic beads are completely separated; then, the Tip pipette head is lowered into the magnetic bead adsorption chamber A to absorb all the waste liquid; then, the lysis liquid chamber B is rotated to the bottom of the pipette component 300, and the pipette component 300 is moved down to release the waste liquid in the Tip pipette head into the lysis liquid chamber B.

[0058] (4) Washing and purification

[0059] First, rotate the first washing liquid chamber C to the bottom of the pipette 300, move the pipette 300 downward, and lower the Tip pipette into the first washing liquid chamber C to absorb 500μL of Buffer RWA 01; then, rotate the magnetic bead adsorption chamber A to the bottom of the pipette 300, release the sample liquid in the Tip pipette into the magnetic bead adsorption chamber A, and use the pipette 300 to blow and mix for several minutes (for example, 2 minutes).

[0060] After the oscillation stops, the magnetic suction component 400 is brought close to the magnetic bead adsorption chamber A to absorb the magnet for several seconds (for example, 20 seconds) until the magnetic beads are completely separated; then, the Tip suction head is lowered into the magnetic bead adsorption chamber A to absorb all the waste liquid; then, the first washing liquid chamber C is rotated to the bottom of the pipetting component 300, the pipetting component 300 is moved down, the waste liquid in the Tip suction head is released into the first washing liquid chamber C, and the magnetic suction component 400 is moved away from the first station.

[0061] Then, the second washing liquid chamber D is rotated to the bottom of the pipette 300, the pipette 300 is moved down, and the Tip pipette is lowered into the second washing liquid chamber D to absorb 500μL of Buffer RWB 01; thereafter, the magnetic bead adsorption chamber A is rotated to the bottom of the pipette 300, the sample liquid in the Tip pipette is released into the magnetic bead adsorption chamber A, and the pipette 300 is used to blow and mix for several minutes (for example, 2 minutes).

[0062] After the oscillation stops, the magnetic suction component 400 is brought close to the magnetic bead adsorption chamber A to absorb the magnet for several seconds (for example, 20 seconds) until the magnetic beads are completely separated; then, the Tip pipette head is lowered into the magnetic bead adsorption chamber A to absorb all the waste liquid; then, the second washing liquid chamber D is rotated to the bottom of the pipette component 300, the pipette component 300 is moved down, the waste liquid in the Tip pipette head is released into the second washing liquid chamber D, and the magnetic suction component 400 is moved away from the first station.

[0063] Then, the washing liquid chamber E is rotated to the bottom of the pipette 300, the pipette 300 is moved down, and the Tip pipette is lowered into the washing liquid chamber E to absorb 500 μL of Buffer RE 01; then, the magnetic bead adsorption chamber A is rotated to the bottom of the pipette 300, and the sample liquid in the Tip pipette is released into the magnetic bead adsorption chamber A. After standing for several seconds (e.g., 5 seconds), the first tool chamber T1 is rotated to the bottom of the pipette 300, the pipette 300 is moved down, and the waste liquid in the Tip pipette is released into the first tool chamber T1.

[0064] (5) Nucleic acid elution

[0065] First, rotate the washing liquid chamber E to the bottom of the pipette 300, move the pipette 300 downward, and lower the Tip pipette into the washing liquid chamber E to absorb 100 μL of Buffer RE 01; then, rotate the magnetic bead adsorption chamber A to the bottom of the pipette 300, and release the sample liquid in the Tip pipette into the magnetic bead adsorption chamber A; then, use the heating element 500 to heat the magnetic bead adsorption chamber A to a preset temperature (for example, 65°C), and use the pipette 300 to blow and mix the magnetic bead adsorption chamber A for several minutes (for example, 5 minutes); then, bring the magnetic suction element 400 close to the magnetic bead adsorption chamber A and magnetize it for several seconds (for example, 20 seconds) until the magnetic beads are completely separated.

[0066] Then, the Tip pipette is lowered into the magnetic bead adsorption chamber A to absorb 5 μL of nucleic acid; then, the freeze-dried ball chamber T3 is rotated to the bottom of the pipette 300, the pipette 300 is moved down, the Tip pipette is lowered into the dry ball chamber T3 and the nucleic acid is released; then, the pipette 300 is used to blow and mix for several seconds (for example, 30 seconds) until the freeze-dried ball is completely dissolved; then, the Tip pipette is lowered into the dry ball chamber T3 to absorb all the solution; then, the detection reaction chamber F is rotated to the bottom of the pipette 300, the pipette 300 is moved down, and the Tip pipette is lowered into the detection reaction chamber F to release the solution.

[0067] If used in conjunction with a PCR instrument, lower the Tip pipette into the dry ball chamber T3 to absorb all the solution, then rotate the detection reaction chamber F to the bottom of the pipette 300, move the pipette 300 downward, and lower the Tip pipette into the detection reaction chamber T4 to release the solution. Take out the PCR tube in the T4 chamber of the reagent kit and put it into the PCR instrument.

[0068] Jump to step (9)

[0069] (6) Constant temperature amplification

[0070] First, rotate the washing liquid chamber E to the bottom of the pipetting part 300, move the pipetting part 300 downward, and lower the Tip pipette into the washing liquid chamber E to absorb 500 μL of Buffer RE 01; then, rotate the detection reaction chamber F to the bottom of the pipetting part 300, lower the Tip pipette until it is tightly matched with the sealing component in the detection reaction chamber F (the solution is not released), and seal the detection reaction chamber F.

[0071] Then, the detection reaction chamber F is heated to a preset temperature (eg, 65° C.) by using the heating element 500 and incubated for several minutes (eg, 20 minutes) to complete the amplification reaction.

[0072] (7) Chromatography reaction

[0073] First, release the amplification product in the Tip pipette into the detection reaction chamber F; then, rotate the washing liquid chamber E to the bottom of the pipette 300, and lower the Tip pipette into the washing liquid chamber E to absorb 500μL of Buffer RE 01; then, rotate the detection reaction chamber F to the bottom of the pipette 300, and release 500μL of Buffer RE 01 in the Tip pipette into the amplification product three times (add 200μL, 200μL and 100μL in sequence), at this time, the mixed product dilution solution immerses the test strip sample well to start the chromatography reaction.

[0074] (8) Result observation

[0075] Observe the color development results of the test strip and record them.

[0076] (9) Medical waste treatment

[0077] Take out the test box 20 from the instrument, put it into a sealed bag, and discard it into a medical waste bin according to local regulations; clean the instrument at the end of each experiment and set it to automatically shut down after ultraviolet disinfection.

[0078] It can be seen that the virus detector 10 provided in this embodiment integrates functions such as sample homogenization, nucleic acid lysis, washing, elution and amplification detection through the cooperation of the operating disk 200 and the pipetting component 300, the magnetic suction component 400 and the heating component 500. Therefore, after the detection box 20 is placed in, the detector can perform a series of operations such as sample homogenization, nucleic acid lysis, washing, elution and amplification detection on the sample in the detection box 20, which can avoid manual transportation of the detection box 20 between different devices and reduce labor intensity, and can also continuously perform virus detection on the sample in the detection box 20 to shorten the detection time.

[0079] It is understandable that the liquid transfer part 300 can achieve the absorption and release of liquid by changing the external air pressure. When the liquid transfer part 300 is loaded with operating tools such as the grinding rod L and the Tip, the solution in the chamber of the detection box 20 can be absorbed into the operating tool or released from the operating tool. Among them, the liquid transfer part can be a liquid transfer pump, and of course the liquid transfer part 300 can also be a disposable liquid transfer gun.

[0080] See also Figure 6 In some embodiments, a pipette tip 310 and a pusher are provided at one end of the pipette 300 close to the operating disk 200. The pipette tip 310 can be inserted into the operating tool in an interference fit manner during the descent process to load the operating tool; the pusher can push the operating tool toward the operating disk 200 until the operating tool falls off the pipette tip 310 to unload the operating tool. When it is necessary to load the grinding rod L or the Tip tip, the first tool cavity T1 or the second tool cavity T2 of the detection box 20 is rotated to the bottom of the pipette 300 by rotating the operating disk 200, and then the pipette 300 is moved downward, and the pipette tip 310 of the pipette 300 is inserted into the top of the grinding rod L or the Tip tip, thereby realizing the loading of the grinding rod L or the Tip tip; when it is necessary to unload the grinding rod L or the Tip tip, the pusher of the pipette 300 pushes the grinding rod L or the Tip tip downward until the grinding rod or the Tip tip falls off from the pipette tip 310, thereby realizing the unloading of the grinding rod or the Tip tip.

[0081] The transfer member 300 is provided with a mechanism in which a power cylinder or a motor cooperates with a transmission component (such as a lead screw and a lead screw nut) to realize the extension and retraction of the push handle.

[0082] In some embodiments, Figure 8 and Fig. 9As shown, a support platform 210 is convexly provided on one side of the operating disk 200 facing the pipetting member 300, and the support platform 210 has a notch 210a along the circumference of the operating disk 200; the heating element 500 includes a resistance wire 510 and a heat-conducting seat 520, the resistance wire 510 is connected to the heat-conducting seat 520, the heat-conducting seat 520 is arranged on the operating disk 200 and accommodated in the notch 210a, and the heat-conducting seat 520 and the support platform 210 are used to receive the detection box 20. Such an arrangement can make the structure of the virus detector 10 compact. It should be noted that the heat-conducting seat 520 is used to receive the first part of the detection box 20, and the support platform 210 is used to carry the second part of the detection box 20, wherein the first part of the detection box 20 refers to the part having the magnetic group reaction chamber A and the detection reaction chamber B, and the second part of the detection box 20 refers to the remaining part of the detection box 20.

[0083] Among them, Figure 8 and Fig. 9 As shown, the number of the resistance wires 510 is set to 2, one of the resistance wires 510 is used to heat the thermal seat 520, and the other resistance wire 510 can be used as a temperature sensor to feedback temperature information.

[0084] In order to prevent the heating element 500 from heating other chambers of the detection box 20, such as Figure 8 and Fig. 9 As shown, the heating element 500 further includes a heat insulating pad 530, which is disposed between the bottom of the heat conducting seat 520 and the top of the operation panel 200; and / or, the heat conducting seat 520 has a heat insulating gap with the support platform 210 along the circumference of the operation panel 200. By providing a heat insulating gap between the heat conducting seat 520 and the support platform 210, the installation of the heating element 500 is also facilitated.

[0085] It should be noted that if Figure 4 As shown, the lengths of the multiple chambers of the detection box 20 are different. For example, the lengths of the sample chamber S, the first tool chamber T1, the second tool chamber T2, the eluent chamber E, the magnetic bead adsorption chamber A and the detection reaction chamber F are longer than those of other chambers. In order to stably receive the detection box 20, Figure 8 and Fig. 9 As shown, a plurality of first limiting cavities 210b are provided on the support platform 210 of the operation panel 200, and each first limiting cavity 210b is used to accommodate a corresponding cavity on the detection box 20. A plurality of second limiting cavities 520a are also provided on the heat conductive seat 520, and each second limiting cavity 520a is used to accommodate a corresponding cavity on the detection box 20. Among them, an opening 520b is provided on the side of the second limiting cavity 520a for accommodating the magnetic bead adsorption cavity A facing the magnetic attraction member 400, and the opening 520b can be provided for the magnetic attraction member 400 to pass through, so that the magnetic attraction member 400 can fit with the cavity wall of the magnetic bead adsorption cavity A, which is conducive to magnetic attraction.

[0086] In some embodiments, Figure 5 As shown, the box 100 includes a box body 110 and a mounting seat 120 disposed in the box body 110; the mounting seat 120 includes a bottom plate 121 and a support frame 122, the bottom plate 121 is disposed at the bottom of the box 100 and is used for mounting the operating disk 200, and the support frame 122 is used for mounting the liquid transfer component 300 and has a first window 122a for the magnetic suction component 400 to pass through. The box 100 of this structure facilitates the installation of the operating disk 200, the liquid transfer component 300, the magnetic suction component 400 and the heating component 500.

[0087] Among them, Figure 2 As shown, the support frame 122 can be installed on the base 121 through a tripod 123 .

[0088] Furthermore, if Figure 2 and Figure 5 As shown, the box 100 also includes a shielding shell 30 and an openable and closable protective door 140; the shielding shell 30 is covered on the box 100 and is provided with a detection chamber 131 protruding toward the inside of the box 100, the top of the detection chamber 131 has a second window for the pipette 300 to pass through, the bottom of the detection chamber 131 has a third window 131a for exposing the operating disk 200, and the side of the detection chamber 131 has an operating port 131b that communicates with the outside of the box 100; the protective door 140 is arranged at the operating port 131b. When detecting viruses, the protective door 140 can be opened first, the detection box 20 can be placed on the operating disk 200 of the detection chamber 131, and then the protective door 140 can be closed for virus detection; after the virus detection is completed, the protective door 140 can be opened and the detection box 20 can be taken out of the detection chamber 131. By providing the shielding shell 30 and the protective door 140 , the components in the virus detector 10 can be protected and the external foreign matter can be prevented from contaminating the pipette tip 310 of the pipette 300 , thereby ensuring the accuracy of the detection.

[0089] The protective door 140 may be a revolving door or a sliding door.

[0090] In some embodiments, Figure 6 As shown, the virus detector 10 further includes a first driving member 600, which is used to drive the operating disk 200 to rotate; the first driving member 600 includes: a first power member 610 disposed on the box body 100, a rotating shaft rotatably disposed on the box body 100, a driving wheel disposed on the output shaft of the first power member 610, a driven wheel disposed on the rotating shaft, and a conveyor belt 620 tensioned between the driving wheel and the driven wheel, and the rotating shaft is connected to the operating disk 200. When the output shaft of the first power member 610 rotates, the driving wheel also rotates, and the driven wheel can be driven to rotate through the conveyor belt 620, and the rotating shaft rotates accordingly, and then the operating disk 200 can be driven to rotate.

[0091] The first power member 610 may be a stepper motor, which can realize closed-loop control. The first power member 610 may be mounted on the chassis 121 of the mounting base 120 via the bracket 170. It should be noted that: Figure 6 Only the output shaft of the first power member 610 is shown, and the main body of the first power member 610 is not shown.

[0092] In some embodiments, Figure 7 As shown, the virus detector 10 further includes a second driving member 700, which is used to drive the liquid transfer member 300 to move up and down; the second driving member 700 includes: a second power member 710 disposed on the housing 100, a lead screw 720 supported on the housing 100 and connected to the output shaft of the second power member 710, a lead screw nut 730 disposed on the lead screw 720, and a first connecting member 740 connected to the lead screw nut 730 and the liquid transfer member 300. When the output shaft of the second power member 710 rotates, the lead screw 720 also rotates accordingly, and the lead screw nut 730 rotates up and down along the lead screw 720, thereby driving the liquid transfer member 300 to move up and down.

[0093] The magnetic attraction member 400 may be a magnet or an electromagnetic element.

[0094] The second power member 710 may be a stepper motor, which can realize closed-loop control. The second power member 710 may be installed on the side of the support frame 122 facing away from the operation panel 200, while the liquid transfer member 300 may be installed on the side of the support frame 122 facing the operation panel 200. It should be noted that, Figure 7 Only the output shaft of the second power member 710 is shown, and the main body of the second power member 710 is not shown.

[0095] like Figure 7 As shown, a support bearing 761 and a limit bearing 762 are further provided on the side of the support frame 122 facing away from the operating panel 200 . The support bearing 761 is used to support the lead screw 720 , and the limit bearing 762 is used to limit the axial position of the lead screw 720 .

[0096] like Figure 6 As shown, the support frame 122 has a fourth window 122b; Figure 7As shown, the first connecting member 740 includes a first connecting portion 741, a second connecting portion 743 and a transfer portion 742 connected in sequence, the first connecting portion 741 and the transfer portion 742 are both located on the side of the support frame 122 facing away from the operation disk 200, and the second connecting portion 743 passes through the fourth window 122b and is connected to the liquid transfer member 300. Among them, the side of the support frame 122 facing away from the operation disk 200 is also provided with a first guide rail 751, and the side of the transfer portion 742 close to the support frame 122 is provided with a first slider 752, and the first slider 752 can slide along the first guide rail 751. Through the cooperation of the first guide rail 751 and the first slider 752, the liquid transfer member 300 can slide in a directional manner. A position sensor 770 is also provided on the side of the support frame 122 facing away from the operation panel 200, and a first detection sheet 7421 is provided on the adapter 742. When the first detection sheet 7421 is close to the position sensor 770, the position sensor 770 can send position information, and the backend can determine the position of the liquid transfer member 300 according to the position information, so as to prevent the first slider 752 from being separated from the first guide rail 751. The position sensor 770 can be an optical sensor or a proximity sensor.

[0097] In some embodiments, Figure 7 As shown, the virus detector 10 further includes a third driving member 800, which is used to drive the magnetic member 400 to move; the third driving member 800 includes: a power cylinder 810 disposed on the box body 100 and a second connecting member 820 connected to the piston rod of the power cylinder 810, and the second connecting member 820 is also connected to the magnetic member 400. When the piston rod of the power cylinder 810 is extended or retracted, the second connecting member 820 also drives the magnetic member 400 to rotate linearly, thereby approaching or moving away from the operating panel 200.

[0098] The power cylinder 810 may be a hydraulic cylinder or a pneumatic cylinder. The power cylinder 810 may be mounted on a base of the mounting seat 120 , and the power cylinder 810 and the operating panel 200 are distributed on opposite sides of the support frame 122 .

[0099] like Figure 7 As shown, the second connecting member 820 includes a third connecting portion 821 and a fourth connecting portion 822 connected to each other, and the third connecting portion 821 and the fourth connecting portion 822 can both be generally L-shaped structures, wherein the horizontal portion of the third connecting portion 821 is connected to the piston rod of the power cylinder 810, the vertical portion of the third connecting portion 821 is connected to the horizontal portion of the fourth connecting portion 822, and the vertical portion of the fourth connecting portion 822 is connected to the magnetic member 400. Among them, a second guide rail is also provided on the base, and a second slider is provided on the horizontal portion of the fourth connecting portion 822, and the second slider can slide along the second guide rail. Through the cooperation of the second guide rail and the second slider, the magnetic member 400 can slide in a directional manner.

[0100] In some embodiments, Figure 1As shown, a plurality of buttons 150 are disposed on the outside of the box 100; Figure 7 As shown, the virus detector 10 also includes a controller 900 disposed in the housing 100. The controller 900 is used to adjust the working state of the operating panel 200, the liquid transfer component 300, the magnetic component 400 or the heating component 500 after detecting that the button 150 is triggered. Among them, the controller 900 can be connected to the first power member 610 of the first driving member 600, the second power member 710 of the second driving member 700, the power cylinder 810 of the third driving member and the resistance wire 510 of the heating member 500 to control the opening and closing of the first power member 610, the second power member 710, and the power cylinder 810 and control whether the resistance wire 510 is heated, so that the detector can be started with one button and the whole detection process is realized until the result is obtained. It should be noted that the working states of the operating panel 200, the pipetting component 300, and the magnetic component 400 may include the open state and the closed state of the first power component 610 of the first driving component 600, the second power component 710 of the second driving component 700, and the power cylinder 810 of the third driving component; the working state of the heating component 500 may include the power-on state and the power-off state of the resistance wire 510.

[0101] The button 150 can be arranged on the shielding shell 30 of the box body 100. The number of the buttons 150 can be set accordingly according to the requirements, for example, Figure 1 As shown, three buttons 150 are provided on the box 100, and these three buttons 150 are used for starting, stopping, and resetting respectively. After the detection box 20 is sent into the detection chamber 131 of the box 100 and the protective door 140 is closed, the operator can press the start button 150. When the controller 900 detects that the start button 150 is triggered, it will correspondingly control the opening and closing of the first power member 610, the second power member 710, and the power cylinder 810 and control whether the resistance wire 510 is heated, so that the detector starts to detect. When it is necessary to stop the detection midway, the operator can press the stop button 150. When the controller 900 detects that the stop button 150 is triggered, it will correspondingly control the opening and closing of the first power member 610, the second power member 710, and the power cylinder 810 and control whether the resistance wire 510 is heated, so that the detector stops detecting. When the virus detection is completed and the detection box 20 is taken out, the operator can press the reset button 150. When the controller 900 detects that the reset button 150 is triggered, it will control the opening and closing of the first power member 610, the second power member 710, and the power cylinder 810 and control whether the resistance wire 510 is heated, so as to reset the detector and facilitate the next virus detection.

[0102] like Figure 1As shown, in some embodiments, a plurality of warning lights 160 are provided on the outside of the box 100; after the virus detection is completed or when the detector fails, the warning lights 160 may light up or flash, thereby reminding the operator that the virus detection has been completed or the detector has failed.

[0103] The controller 900 is connected to the indicator light 160 by signal so as to adjust the working mode of the indicator light 160 .

[0104] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0105] The above-described embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be construed as limiting the scope of the patent application. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent application shall be subject to the attached claims.

Claims

1. A virus detector, characterized in that: Used to perform virus detection on a sample in a detection box, the detection box having a plurality of chambers arranged along its circumference, the virus detector comprising: A box body, wherein the box body has a first workstation, a second workstation located above the first workstation, and a third workstation located to the side of the first workstation; An operating disk, the operating disk is used to receive the detection box, and the operating disk is rotatably disposed at the first station, so that any of the chambers of the detection box can be rotated to the bottom of the second station or to the side of the third station; a liquid transfer member, which is disposed at the second station so as to be movable up and down, and is capable of loading the operating tool in the chamber or unloading the operating tool into the corresponding chamber; A magnetic attraction member, which is slidably disposed at the third station and can be moved close to or away from the chamber for magnetic bead adsorption, so as to adsorb and separate the magnetic beads in the chamber for magnetic bead adsorption; and A heating element is disposed on the operation disk and is capable of heating the chamber used for amplification reaction and the chamber used for magnetic bead adsorption.

2. The virus detector according to claim 1, characterized in that: The end of the liquid transfer component close to the operating disk is provided with a liquid transfer gun head and a retractable push handle; The pipette tip can be inserted into the operating tool in an interference fit manner during the descending process to load the operating tool; The pusher can push the operating tool toward the operating disk until the operating tool falls off the pipette tip to unload the operating tool.

3. The virus detector according to claim 1, characterized in that: A bearing platform is convexly provided on one side of the operation disk facing the liquid transfer member, and the bearing platform has a notch along the circumference of the operation disk; The heating element comprises a resistance wire and a heat-conducting seat, the resistance wire is connected to the heat-conducting seat, the heat-conducting seat is arranged on the operation panel and accommodated in the notch, and the heat-conducting seat and the supporting platform are used to receive the detection box.

4. The virus detector according to claim 3, characterized in that: The heating element further includes a heat insulating pad, which is arranged between the bottom of the heat conducting seat and the top of the operating panel; and / or, The heat-conducting seat has a heat-insulating interval with the supporting platform along the circumferential direction of the operating panel.

5. The virus detector according to claim 1, characterized in that: The box body comprises a box body main body and a mounting seat arranged in the box body main body; The mounting base includes a chassis and a support frame. The chassis is arranged at the bottom of the box and is used for mounting the operating panel. The support frame is used for mounting the liquid transfer component and has a first window for the magnetic suction component to pass through.

6. The virus detector according to claim 5, characterized in that: The box body also includes a shielding shell and an openable and closable protective door; The shielding shell cover is mounted on the box body and is provided with a detection chamber protruding toward the inside of the box body, the top of the detection chamber is provided with a second window for the pipetting component to pass through, the bottom of the detection chamber is provided with a third window for exposing the operating panel, and the side of the detection chamber is provided with an operating port communicating with the outside of the box body; the protective door is provided at the operating port.

7. The virus detector according to any one of claims 1 to 6, characterized in that: The virus detector further includes a first driving member, which is used to drive the operating disk to rotate; The first driving member includes a first power member arranged on the box body, a rotating shaft rotatably arranged on the box body, a driving wheel arranged on the output shaft of the first power member, a driven wheel arranged on the rotating shaft, and a conveyor belt tensioned between the driving wheel and the driven wheel, and the rotating shaft is connected to the operating panel.

8. The virus detector according to any one of claims 1 to 6, characterized in that: The virus detector further comprises a second driving member, which is used to drive the liquid transfer member to move up and down; The second driving member includes a second power member arranged on the box body, a screw supported on the box body and connected to the output shaft of the second power member, a screw nut arranged on the screw, and a first connecting member connected to the screw nut and the pipetting member.

9. The virus detector according to any one of claims 1 to 6, characterized in that: The virus detector further comprises a third driving member, and the third driving member is used to drive the magnetic attraction member to move; The third driving member includes a power cylinder arranged on the box body and a second connecting member connected to the piston rod of the power cylinder, and the second connecting member is also connected to the magnetic attraction member.

10. The virus detector according to any one of claims 1 to 6, characterized in that: A plurality of buttons are arranged on the outer side of the box; The virus detector also includes a controller disposed in the housing, and the controller adjusts the working state of the operating disk, the pipetting component, the magnetic component or the heating component after detecting that the button is triggered.

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