Novel pipette tip suitable for collecting organoids

Abstract

The novel pipette tip comprises a connecting part and a suction nozzle, the suction nozzle is arranged at one end of the connecting part, and the suction nozzle and the connecting part are communicated with each other; a slow flow structure is arranged in the connecting part; the suction nozzle is conical, an opening is formed in the lower portion of the suction nozzle, the opening in the lower end of the suction nozzle is composed of a flat opening part and an oblique opening part, and the oblique opening part inclines from the middle of the bottom end of the suction nozzle to the middle of the suction nozzle. The device has the beneficial effects that suction force is adjusted in the pipetting and organoid collection process, so that control over pipetting and organoid collection is more convenient and faster; the partition plate is arranged in the connecting part, so that a sealing effect can be achieved when the connecting part is connected with the pipette, and meanwhile, the flow speed of liquid in the gun head is reduced through the flow slowing hole during pipetting and organoid collection operation, so that damage to the organoid is reduced; by arranging the double threads in the suction nozzle, after the suction nozzle is in contact with liquid, the liquid can be prevented from flowing back by utilizing the tension of the liquid, and the organoid and the pipettor are protected.

Description

Technical Field

[0001] The utility model relates to the technical field of experimental tools, and in particular to a novel pipette tip suitable for collecting organoids. Background Art

[0002] One of the key advances in stem cell research over the past decade has been the development of organoid systems. Organoids are three-dimensional (3D) cell cultures that have highly similar histological features to corresponding human organs and can reproduce some of the physiological functions of organs. These 3D systems can replicate the complex spatial morphology of differentiated tissues and can demonstrate cell-cell and cell-matrix interactions. Organoids have their own unique experimental advantages. They remove the confounding variables that may be introduced by animal models, but provide a higher degree of complexity compared to homogenized 2D cell cultures. By combining a high level of physiological relevance with the convenience of in vitro operations, in many cases, organoids have the potential to assist or replace in vitro experiments using primary cells or immortalized cell lines and animals. In addition, organoids have a high degree of genetic stability in culture and maintain the genotype and phenotype of the source tissue. Therefore, organoids have broad application prospects in many fields such as stem cells and development, regenerative medicine, disease research, drug development, and precision medicine.

[0003] With the development of organoid technology, existing conventional pipettes and pipette tips are difficult to meet the current experimental operations of organoids: 1. Conventional pipette tips are flat, and it is difficult to control the adsorption force when changing the liquid and collecting organoids, resulting in difficulty in controlling the amount of liquid change and organoid collection;

[0004] 2. Conventional pipette tips have no shielding or sealing inside, which causes the organoids to be damaged due to excessive flow rate after being sucked into the tip. The contact sealing between the tip and the pipette cannot be guaranteed, which can lead to leakage. Utility Model Content

[0005] The purpose of the present invention is to provide a new type of pipette tip suitable for organoid collection in order to solve the above-mentioned problems. The preferred technical solution among the many technical solutions provided by the present invention has the following technical effects: good sealing, convenient operation, convenient and easy control of pipetting and organoid collection operations, etc., as described below for details.

[0006] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0007] The utility model provides a novel pipette tip suitable for collecting organoids, comprising a connecting portion and a suction nozzle, wherein the suction nozzle is arranged at one end of the connecting portion, and the two are connected;

[0008] A slow flow structure is provided inside the connecting portion;

[0009] The suction nozzle is conical and has an opening at the bottom. The opening at the lower end of the suction nozzle consists of a flat opening and an oblique opening. The oblique opening is inclined from the middle of the bottom end of the suction nozzle to the middle of the suction nozzle.

[0010] Preferably, the inner wall of the suction nozzle is provided with a tension-increasing structure for increasing the tension of the liquid, and the tension-increasing structure extends from the upper side of the oblique mouth portion to the upper end of the suction nozzle.

[0011] Preferably, the expansion structure is a plurality of annular patterns formed on the inner wall of the nozzle.

[0012] Preferably, the expansion structure is a double helix pattern formed on the inner wall of the nozzle.

[0013] Preferably, a boss supporting the slow-flow structure is arranged on the inner side of the connecting portion, and the boss is an annular structure and protrudes from the inner wall of the connecting portion.

[0014] Preferably, the slow-flow structure is a flexible baffle, which is circular and has a clearance fit with the inner wall of the connecting portion.

[0015] Preferably, the partition comprises a circular plate body, and a plurality of slow-flow holes are formed on the plate body.

[0016] Preferably, a central hole is formed at the center of the partition, and a plurality of slow-flow holes are evenly distributed on the outside of the central hole; the slow-flow holes are C-shaped holes.

[0017] In summary, the beneficial effects of the utility model are: 1. By arranging a flat mouth portion and an oblique mouth portion at the tip of the suction nozzle, the suction force of the suction nozzle can be adjusted by adjusting the relative position of the oblique mouth portion and the liquid surface, thereby adjusting the suction force during the process of pipetting and organoid collection, making the control of pipetting and organoid collection more convenient;

[0018] 2. By setting a partition inside the connection part, it can play a sealing role when the connection part is connected to the pipette. At the same time, during the pipetting and organoid collection operations, the liquid flow rate inside the pipette tip is reduced through the slow flow hole to reduce the damage to the organoid;

[0019] 3. By setting a double helix pattern inside the nozzle, after contact with the liquid, the liquid tension can be used to prevent liquid reflux and protect the organoid and pipette. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 It is a main structural schematic diagram of the utility model;

[0022] Figure 2 yes Figure 1 Schematic diagram of the cross-sectional structure along the AA direction;

[0023] Figure 3 It is a left-side structural schematic diagram of the utility model;

[0024] Figure 4 It is a schematic diagram of the top view structure of the utility model;

[0025] Figure 5 It is a three-dimensional structural schematic diagram of the utility model;

[0026] Figure 6 It is a three-dimensional structural schematic diagram of the partition of the utility model.

[0027] The following are the descriptions of the reference numerals:

[0028] 1. Connecting part; 2. Suction nozzle; 3. Flat mouth; 4. Oblique mouth; 5. Double helix pattern; 6. Boss; 7. Partition; 71. Plate body; 72. Center hole; 73. Slow flow hole. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical solution and advantages of the utility model clearer, the technical solution of the utility model will be described in detail below. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other implementation methods obtained by ordinary technicians in this field without creative work belong to the scope of protection of the utility model.

[0030] See also Figure 1-Figure 6 As shown, the utility model provides a novel pipette tip suitable for collecting organoids, comprising a connecting portion 1 and a suction nozzle 2, wherein the suction nozzle 2 is arranged at one end of the connecting portion 1, and the two are connected;

[0031] A slow-flow structure is provided inside the connection part 1; a boss 6 supporting the slow-flow structure is provided inside the connection part 1, the boss 6 is an annular structure and protrudes from the inner wall of the connection part 1; the slow-flow structure is a flexible baffle 7, the baffle 7 is circular and has a clearance fit with the inner wall of the connection part 1; the baffle 7 includes a circular plate body 71, and a plurality of slow-flow holes 73 are formed on the plate body 71;

[0032] A central hole 72 is formed at the center of the partition 7, and a plurality of slow-flow holes 73 are evenly distributed on the outside of the central hole 72; the slow-flow hole 73 is a C-shaped hole. Since the surface area of ​​the plate body 71 is limited, when the slow-flow hole 73 is set to a C-shaped structure, the area ratio of the slow-flow hole 73 can be increased while the number of holes is set to be small, thereby providing a slow-flow effect while reducing the impact on the flow of liquid and organoids to avoid damage to the similar items; the suction nozzle 2 is conical and has an opening at the bottom. The opening at the lower end of the suction nozzle 2 is composed of a flat mouth portion 3 and an oblique mouth portion 4. The oblique mouth portion 4 is inclined from the middle of the bottom end of the suction nozzle 2 to the middle of the suction nozzle 2. After such a setting, when absorbing liquid and organoids, the suction nozzle 2 can be first inserted vertically into the liquid surface. At this time, the oblique mouth portion 4 is not completely closed. The exposure size of the oblique mouth portion 4 can be adjusted by adjusting the depth of the suction nozzle 2 inserted into the liquid or the angle of coordination with the liquid surface, thereby adjusting the suction force inside the suction nozzle 2, making the adsorption operation easier to control;

[0033] The inner wall of the nozzle 2 is provided with a tension-increasing structure for increasing the tension of the liquid. The tension-increasing structure extends from the upper part of the oblique mouth 4 to the upper end of the nozzle 2. The extension structure is an inwardly concave or outwardly convex pattern formed on the inner wall of the nozzle 2 to increase the contact area between the liquid and the inner wall of the nozzle 2, thereby preventing the liquid from flowing back by increasing the tension.

[0034] As an implementation of the expansion structure, the expansion structure is a plurality of annular patterns formed on the inner wall of the nozzle 2, and the plurality of annular patterns are parallel to each other and evenly distributed along the axis direction of the nozzle 2;

[0035] As another implementation of the expansion structure, the expansion structure is a double helix pattern 5 formed on the inner wall of the suction nozzle 2, and the double helix pattern 5 is two spirally ascending and mutually interlaced arcs.

[0036] By adopting the above structure, by arranging a flat mouth portion 3 and an oblique mouth portion 4 at the tip of the suction nozzle 2, the suction force of the suction nozzle 2 can be adjusted by adjusting the relative position of the oblique mouth portion 4 and the liquid surface. During operation, the flat mouth portion 3 contacts the organoid to absorb the organoid, and the suction force is adjusted during the pipetting and organoid collection process, so that the control of pipetting and organoid collection is more convenient; by arranging a partition plate 7 inside the connecting portion 1, it can be used as a sealing pad when the connecting portion 1 is connected to the pipette gun to play a sealing role. At the same time, during the pipetting and organoid collection operations, the slow flow hole 73 is used to reduce the liquid flow rate inside the gun head to reduce the damage to the organoid; by arranging a double helix pattern 5 inside the suction nozzle 2, after contacting with the liquid, the specific surface area of ​​the liquid in contact with the inside of the gun head can be increased, thereby increasing the tension of the liquid, making the contact between the liquid and the inner wall of the suction nozzle 2 more stable, and the liquid tension can be used to avoid liquid reflux, thereby protecting the organoid and the pipette.

[0037] The above is only a specific implementation of the utility model, but the protection scope of the utility model is not limited thereto. Any technician familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed by the utility model, which should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope of the claims.

Claims

1. A novel pipette tip suitable for organoid collection, characterized in that: It comprises a connecting portion (1) and a suction nozzle (2), wherein the suction nozzle (2) is arranged at one end of the connecting portion (1), and the two are connected; A slow-flow structure is provided inside the connecting portion (1); The suction nozzle (2) is conical and has an opening at the bottom. The opening at the bottom of the suction nozzle (2) consists of a flat opening portion (3) and an oblique opening portion (4). The oblique opening portion (4) is inclined from the middle of the bottom end of the suction nozzle (2) towards the middle of the suction nozzle (2).

2. A novel pipette tip suitable for organoid collection according to claim 1, characterized in that: The inner wall of the suction nozzle (2) is provided with a tension-increasing structure for increasing the tension of the liquid, and the tension-increasing structure extends from the upper side of the oblique mouth portion (4) to the upper end of the suction nozzle (2).

3. A novel pipette tip suitable for organoid collection according to claim 2, characterized in that: The expansion structure is a plurality of annular patterns formed on the inner wall of the suction nozzle (2).

4. A novel pipette tip suitable for collecting organoids according to claim 2, characterized in that: The expansion structure is a double helical pattern (5) formed on the inner wall of the suction nozzle (2).

5. A novel pipette tip suitable for organoid collection according to claim 1, characterized in that: A boss (6) supporting the slow-flow structure is arranged on the inner side of the connecting portion (1); the boss (6) is an annular structure and protrudes from the inner wall of the connecting portion (1).

6. A novel pipette tip suitable for organoid collection according to claim 1, characterized in that: The slow-flow structure is a flexible baffle (7), the baffle (7) is circular and is gap-matched with the inner wall of the connecting portion (1).

7. A novel pipette tip suitable for organoid collection according to claim 6, characterized in that: The partition plate (7) comprises a circular plate body (71), and a plurality of slow-flow holes (73) are formed on the plate body (71).

8. A novel pipette tip suitable for organoid collection according to claim 7, characterized in that: A central hole (72) is formed at the center of the partition (7), and a plurality of slow-flow holes (73) are evenly distributed outside the central hole (72); the slow-flow holes (73) are C-shaped holes.

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