Multilayer tissue engineering biological scaffold for tendon repair

Abstract

The utility model discloses a multi-layer tissue engineering biological scaffold for tendon repair, which comprises a biological scaffold which is of a cylindrical structure and comprises a biological membrane layer which is the outermost layer of the biological scaffold, a biological membrane layer which is the outer layer of the biological scaffold, and a biological membrane layer which is the inner layer of the biological scaffold. The first bracket layer is fixedly arranged on the inner wall of the biological membrane layer; a plurality of active particles disposed on the top of the first stent layer; the main body structure adding layer is fixedly arranged on the inner wall of the first bracket layer; the second bracket layer is fixedly arranged on the inner wall of the main body structure adding layer; the plurality of lysozyme bodies are arranged at the top of the second bracket layer. The problems of insufficient mechanical strength, poor hydrophilicity, poor adhesion and poor histocompatibility of common materials can be solved, and the mechanical property and the biological activity of the material are improved.

Description

Technical Field

[0001] The utility model relates to a multilayer tissue engineering biological scaffold for tendon repair, belonging to the technical field of biomedicine. Background Art

[0002] The treatment of tendon injury is one of the most difficult in the human body. The difficulty mainly comes from the tendon tissue itself, such as few tendon cells, low blood supply, and very low metabolic rate, which leads to very limited self-repair ability.

[0003] At present, the main method for treating tendon injuries is tendon transplantation, which is divided into three categories: biological tendon transplantation, artificial tendon transplantation and tissue engineering scaffold materials. Among them, biological tendons are divided into autologous, allogeneic and xenogeneic, and their disadvantages are narrow sources, low compatibility, ethical issues, immune rejection, etc.; artificial tendon materials include alloys, plastics, silicone, chitin, collagen, etc., but the use of these materials has problems such as being unable to be absorbed by the human body, slow healing of sutures, significant rejection reactions, adhesions, easy infection, and insufficient mechanical properties; tissue engineering scaffold materials include fibrin, chitin, PLA, PGA, etc., but different materials also have problems such as insufficient mechanical strength, poor hydrophilicity, poor adhesion, and poor tissue compatibility. Utility Model Content

[0004] The utility model designs and develops a multilayer tissue engineering biological scaffold for tendon repair, which can overcome the problems of insufficient mechanical strength, poor hydrophilicity, poor adhesion and poor tissue compatibility of common materials and improve their mechanical properties and biological activity.

[0005] The technical solution provided by the utility model is:

[0006] A multilayer tissue engineering bioscaffold for tendon repair, the bioscaffold is a cylindrical structure and comprises:

[0007] A biofilm layer, which is the outermost layer of the bioscaffold;

[0008] A first support layer, which is fixedly disposed on the inner wall of the biofilm layer;

[0009] a plurality of active particles disposed on top of the first support layer;

[0010] A main structure layer, which is fixedly arranged on the inner wall of the first support layer;

[0011] A second support layer, which is fixedly arranged on the inner wall of the main structure layer;

[0012] A plurality of lysozymes are disposed on top of the second support layer.

[0013] Preferably, the material of the biofilm layer is human dermal tissue extract.

[0014] Preferably, the first support layer and the second support layer are made of the same material, both of which are sodium alginate hydrogel.

[0015] Preferably, the material of the main structure layer is nanocellulose.

[0016] Preferably, the material of the active particles is PLGA nanomaterial.

[0017] Preferably, the active particles are connected to the first scaffold layer by physical cross-linking.

[0018] Preferably, the biofilm layer, the first support layer, the main structure layer and the second support layer are fixedly connected by lamination.

[0019] Beneficial effects of the utility model:

[0020] The multilayer tissue engineering biological scaffold for tendon repair provided by the utility model is a four-layer structure tissue engineering scaffold based on hydrogel, which is compounded with nanocellulose and polymer microspheres to improve mechanical strength, and then immobilized enzymes are added to improve biological activity. It has similar softness and flexibility to biological tissues, good biodegradability and compatibility, and improves mechanical properties and biological activity.

[0021] The mechanical strength and stress of the material are enhanced by using a multilayer structure of composite nanocellulose and physically cross-linked PLGA particles. The immunogenicity is reduced by adding a biofilm to prevent adhesion during the recovery process and increase biological activity. The risk of infection is reduced by adding immobilized phage lytic enzymes. The sodium alginate scaffold as the main frame has strong hydrophilicity and tissue compatibility. The shortcomings of currently commonly used materials, such as insufficient mechanical strength, poor hydrophilicity, poor adhesion, and poor tissue compatibility, are solved. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a schematic diagram of the three-dimensional structure of the multilayer tissue engineering biological scaffold for tendon repair described in the utility model.

[0023] Figure 2 This is a front view of the multilayer tissue engineering biological scaffold for tendon repair described in the utility model.

[0024] Figure 3 This is a schematic structural diagram of the second support layer described in the present utility model. DETAILED DESCRIPTION

[0025] The present invention will be further described in detail below in conjunction with the accompanying drawings so that those skilled in the art can implement the invention with reference to the description.

[0026] like Figure 1-3 As shown, the utility model provides a multilayer tissue engineering biological scaffold for tendon repair, comprising: a biofilm layer 1, a first scaffold layer 2a, a main structure layer 3, a second scaffold layer 2b, active particles 4, and lysozyme 5.

[0027] like Figure 1-2 As shown, the bioscaffold is a multi-layered cylinder as a whole, and includes, from the outside to the inside, the following connected in sequence: a biofilm layer 1, a first scaffold layer 2a, a main structure layer 3, and a second scaffold layer 2b, wherein the biofilm layer 1 is the outermost layer, the first scaffold layer 2a is fixedly arranged on the inner wall of the biofilm layer 1, and a plurality of active particles 4 are fixedly arranged on the top of the first scaffold layer 2a, the main structure layer 3 is fixedly arranged on the inner wall of the first scaffold layer 2a, the second scaffold layer 2b is fixedly arranged on the inner wall of the main structure layer 3, and a plurality of lysozymes 5 are fixedly arranged on the top of the second scaffold layer 2b.

[0028] In the present invention, as a preferred embodiment, the biofilm layer 1 adopts the decellularized component technology, and the ECM extracted from the human dermis tissue can increase the biological activity of the material, reduce immune rejection, and prevent adhesion when the tendon heals.

[0029] In the present invention, as a preferred embodiment, the material of the first support layer 2a and the second support layer 2b is sodium alginate, which is manufactured by electrospinning technology, and maintains the safety of the overall material with its strong permeability, water absorption and self-degradability.

[0030] In the present invention, as a preferred embodiment, the material of the active particles 4 is PLGA nanoparticles, the active particles 4 and the first scaffold layer 2a are physically cross-linked, the active particles are cross-linked with the sodium alginate hydrogel, and the particles are distributed on it, which can improve the mechanical properties, such as Figure 3 shown.

[0031] In the present invention, as a preferred embodiment, the material of the main structure additional layer 3 is nanocellulose, which can give full play to the toughness of the material, increase the mechanical properties, make the artificial tendon tougher, and enhance the stress.

[0032] In the present invention, as a preferred embodiment, the lysozyme 5 is an immobilized phage lytic enzyme, which reduces the body's inflammatory response and the risk of infection by controlling the release of the phage lytic enzyme.

[0033] In the present invention, as a preferred embodiment, the porous three-dimensional structure of the material is achieved by freeze-drying, and each layer of material is bonded by an adhesive or covalent cross-linking.

[0034] The four-layered tissue engineering scaffold based on hydrogel has its mechanical strength and stress enhanced by composite nanocellulose and physically cross-linked PLGA particles. The immunogenicity is reduced by adding a biofilm to prevent adhesion during the recovery process and increase biological activity. The risk of infection is reduced by adding immobilized phage lytic enzymes. The sodium alginate scaffold as the main frame has strong hydrophilicity and tissue compatibility. It solves the shortcomings of currently commonly used materials, such as insufficient mechanical strength, poor hydrophilicity, poor adhesion, and poor tissue compatibility.

[0035] Although the implementation scheme of the utility model has been disclosed as above, it is not limited to the applications listed in the specification and implementation modes. It can be fully applied to various fields suitable for the utility model. For those familiar with the art, additional modifications can be easily implemented. Therefore, without departing from the general concept defined by the claims and the scope of equivalents, the utility model is not limited to the specific details and the illustrations shown and described herein.

Claims

1. A multilayer tissue engineering bioscaffold for tendon repair, characterized in that: The bio-scaffold is a cylindrical structure, and comprises, from outside to inside: A biofilm layer, which is the outermost layer of the bioscaffold; A first support layer, which is fixedly disposed on the inner wall of the biofilm layer; a plurality of active particles disposed on top of the first support layer; A main structure layer, which is fixedly arranged on the inner wall of the first support layer; A second support layer, which is fixedly arranged on the inner wall of the main structure layer; A plurality of lysozymes are disposed on top of the second support layer.

2. The multilayer tissue engineering bioscaffold for tendon repair according to claim 1, characterized in that: The material of the biofilm layer is human dermal tissue extract.

3. The multilayer tissue engineering bioscaffold for tendon repair according to claim 2, characterized in that: The first support layer and the second support layer are made of the same material, which is sodium alginate hydrogel.

4. The multilayer tissue engineering bioscaffold for tendon repair according to claim 3, characterized in that: The material of the main structure layer is nanocellulose.

5. The multilayer tissue engineering bioscaffold for tendon repair according to claim 4, characterized in that: The material of the active particles is PLGA nanomaterial.

6. The multilayer tissue engineering bioscaffold for tendon repair according to claim 5, characterized in that: The active particles are connected to the first support layer through physical cross-linking.

7. The multilayer tissue engineering bioscaffold for tendon repair according to claim 6, characterized in that: The biofilm layer, the first support layer, the main structure layer and the second support layer are fixedly connected by lamination.

Images