Efficient screening device for bacillus / escherichia coli engineering strains
By installing a clamped fixing assembly between the deep-hole plate and the bottom plate, the problem of leakage of screening solution caused by the gap between the deep-hole plate and the bottom plate is solved, improving the screening effect and simplifying the disassembly process.
Patent Information
- Application Number
- CN202421058308.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-15
- Publication Date
- 2025-02-28
- Estimated Expiration
- 2034-05-15
AI Technical Summary
When the deep-hole plate and the base plate are installed, there may be gaps, causing the screening solution to enter other storage tanks from the gap, reducing the screening effect.
An efficient screening device for Bacillus/Escherichia coli engineering strains was designed. By installing a snap-fit fixing component between the deep-well plate and the bottom plate, including the upper side plate, the lower side plate, the movable groove and the insertion plate, the deep-well plate is closely connected to the bottom plate and preventing the solution from entering through the gap.
It effectively prevents the solution from entering other storage tanks through the gap, improves the screening effect, and simplifies the disassembly process of deep-hole plates and bottom plates.
Smart Images
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Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of screening devices, in particular to a high-efficiency screening device for Bacillus / Escherichia coli engineering strains. Background Art
[0002] Deep-well plates are laboratory consumables used for high-throughput experiments, and are commonly used in biochemical experiments, molecular biology experiments, and other fields. The depth of each hole in a deep-well plate is deeper than that of an ordinary well plate, and can accommodate a larger volume of reaction liquid. Deep-well plates are widely used in high-throughput screening experiments. By adding reactants or compounds under different conditions to different wells in a deep-well plate, multiple experiments can be performed simultaneously to quickly screen out substances with specific properties or activities. During screening, a mounting base plate is used at the bottom of the deep-well plate, and a loading slot is provided on the base plate, which corresponds one-to-one to the holes on the deep-well plate. However, the following defects still exist:
[0003] When the deep-well plate and the bottom plate are installed, there may be a gap between the deep-well plate and the bottom plate. When screening is performed, the screening solution may enter other containing tanks through the gap, resulting in poor screening effect. Utility Model Content
[0004] In view of the above situation, in order to overcome the defects of the prior art, the utility model provides a Bacillus / Escherichia coli engineering strain efficient screening device, which effectively solves the problem that the screening solution may enter other containing tanks through the gap, resulting in poor screening effect.
[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a highly efficient screening device for Bacillus / Escherichia coli engineering strains, comprising a deep-well plate, on which hole slots are evenly opened, and screening holes are evenly opened on the bottom wall of the hole slots, a bottom plate is provided below the deep-well plate, and holding slots are evenly opened on the bottom plate, the holding slots correspond to the hole slots one by one, and the holding slots are used to hold substances filtered in the hole slots, a clamping fixing assembly is installed between the deep-well plate and the hole slots, positioning slots are opened at the four corners of the top of the bottom plate, and positioning blocks are installed at the four corners of the bottom end of the deep-well plate, and the positioning blocks are inserted into the interior of the positioning slots;
[0006] The clamping and fixing assembly includes an upper side plate symmetrically mounted on both sides of the deep-hole plate, a slot is provided inside the upper side plate, and one end of the slot penetrates to a side of the upper side plate away from the deep-hole plate;
[0007] The clamping and fixing assembly also includes lower side plates symmetrically installed on both sides of the hole groove, and the lower side plates are located below the upper side plates.
[0008] Preferably, a movable groove is opened inside the lower side plate, a movable block is slidably installed inside the movable groove, a cross bar is installed on the side of the movable block away from the bottom plate, the end of the cross bar passes through the side of the lower side plate away from the bottom plate, an end plate is fixedly installed on the end of the cross bar, and an insertion plate is fixedly installed on the top of the end plate. When the insertion plate is inserted into the slot, the bottom wall of the deep hole plate is in close contact with the top wall of the bottom plate.
[0009] Preferably, the side of the movable block close to the bottom plate contacts the inner wall of the movable groove close to the bottom plate, a first spring is symmetrically installed on the side of the movable block away from the bottom plate, one end of the first spring is fixedly connected to the inner wall of the movable groove away from the bottom plate, and a handle is installed on the side of the end plate away from the lower side plate.
[0010] Preferably, a longitudinal slide groove is provided inside the plug plate, the top end of the longitudinal slide groove extends to the top of the plug plate, a card block is slidably installed inside the longitudinal slide groove, the top end of the card block is inserted into the inside of the card groove, the card groove is provided on the inner top wall of the slot, a pressure inclined surface is provided on the top end of the card block, and the bottom end of the pressure inclined surface is inclined downward toward the side of the bottom plate.
[0011] Preferably, a transverse slide groove is provided inside the plug plate, the transverse slide groove is located on the side of the longitudinal slide groove away from the deep hole plate, a connecting groove is provided on the side of the transverse slide groove close to the longitudinal slide groove, a transverse slide plate is slidably installed inside the transverse slide groove, a connecting rod is provided between the transverse slide plate and the block, both ends of the connecting rod are respectively hinged to the transverse slide plate and the block, and the end of the connecting rod close to the block is tilted downward.
[0012] Preferably, a second spring is symmetrically installed on one side of the transverse slide close to the block, one end of the second spring is fixedly connected to the inner wall of one end of the transverse slide close to the longitudinal slide, and a push rod is installed on the side of the transverse slide away from the block, and the push rod penetrates to the side of the insert plate away from the deep hole plate.
[0013] Compared with the prior art, the beneficial effects of the utility model are:
[0014] During operation, when the deep-well plate is installed above the bottom plate, the insert plate is inserted into the slot to fix the deep-well plate and the bottom plate. At the same time, under the limiting effect of the insert plate, the bottom wall of the deep-well plate is tightly attached to the top wall of the bottom plate, so that the hole slots and the containing slots correspond to each other one by one, and the solution is prevented from entering other containing slots through the gap between the deep-well plate and the bottom plate, thereby improving the screening effect.
[0015] During operation, when the inserted plate is inserted into the slot, the card block can be stuck in the slot under the pressure of the compressed inclined surface and the elastic force of the second spring, thereby improving the tight fit stability of the deep hole plate and the bottom plate. At the same time, pushing the push rod can disengage the card block from the slot, making it convenient for the disassembly of the deep hole plate and the bottom plate. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. They are used together with the embodiments of the present invention to explain the present invention and do not constitute a limitation to the present invention.
[0017] In the attached picture:
[0018] Figure 1 This is a schematic diagram of the structure of a Bacillus / Escherichia coli engineering strain efficient screening device of the utility model;
[0019] Figure 2 This is a schematic diagram of the deep hole plate structure of the utility model;
[0020] Figure 3 This is a schematic diagram of the bottom plate structure of the utility model;
[0021] Figure 4 This is a schematic diagram of the structure of the clamping and fixing assembly of the utility model;
[0022] Figure 5 This is a schematic diagram of the internal structure of the plugboard of the utility model.
[0023] In the figure: 1, deep hole plate; 2, hole groove; 3, bottom plate; 4, containing groove; 5, positioning groove; 6, positioning block; 7, clamping and fixing assembly; 701, upper side plate; 702, slot; 703, lower side plate; 704, movable groove; 705, movable block; 706, cross bar; 707, end plate; 708, plug plate; 709, handle; 710, first spring; 711, clamping groove; 712, longitudinal slide groove; 713, clamping block; 714, pressure inclined surface; 715, transverse slide groove; 716, connecting groove; 717, transverse slide plate; 718, connecting rod; 719, second spring; 720, push rod. DETAILED DESCRIPTION
[0024] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments; based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.
[0025] Depend on Figure 1-5The utility model relates to an efficient screening device for Bacillus / Escherichia coli engineering strains, comprising a deep well plate 1, on which hole slots 2 are evenly opened, and screening holes are evenly opened on the bottom wall of the hole slots 2, a bottom plate 3 is provided below the deep well plate 1, and holding slots 4 are evenly opened on the bottom plate 3, and the holding slots 4 correspond to the hole slots 2 one by one, and the holding slots 4 are used to hold substances filtered in the hole slots 2, and a clamping fixing component 7 is installed between the deep well plate 1 and the hole slots 2, and positioning slots 5 are opened at the four corners of the top of the bottom plate 3, and positioning blocks 6 are installed at the four corners of the bottom end of the deep well plate 1, and the positioning blocks 6 are inserted into the interior of the positioning slots 5;
[0026] The clamping and fixing assembly 7 includes an upper side plate 701 symmetrically installed on both sides of the deep hole plate 1, a slot 702 is provided inside the upper side plate 701, one end of the slot 702 penetrates to the side of the upper side plate 701 away from the deep hole plate 1, and the clamping and fixing assembly 7 also includes a lower side plate 703 symmetrically installed on both sides of the hole groove 2, the lower side plate 703 is located below the upper side plate 701, a movable groove 704 is provided inside the lower side plate 703, a movable block 705 is slidably installed inside the movable groove 704, a cross bar 706 is installed on the side of the movable block 705 away from the bottom plate 3, the end of the cross bar 706 penetrates to the side of the lower side plate 703 away from the bottom plate 3, an end plate 707 is fixedly installed on the end of the cross bar 706, and a plug plate is fixedly installed on the top of the end plate 707 When the insert plate 708 is inserted into the slot 702, the bottom wall of the deep well plate 1 is in close contact with the top wall of the bottom plate 3. When the deep well plate 1 is installed above the bottom plate 3, the insert plate 708 is inserted into the slot 702 to clamp and fix the deep well plate 1 and the bottom plate 3. At the same time, under the limiting action of the insert plate 708, the bottom wall of the deep well plate 1 is in close contact with the top wall of the bottom plate 3, so that the hole groove 2 and the containing groove 4 have a one-to-one correspondence effect, avoiding the solution from entering other containing grooves 4 through the gap between the deep well plate 1 and the bottom plate 3, thereby improving the screening effect. The side of the movable block 705 close to the bottom plate 3 is in contact with the inner wall of the side of the movable groove 704 close to the bottom plate 3. The side of the movable block 705 away from the bottom plate 3 is symmetrically installed with a first spring 710. One end of the first spring 710 is in contact with the bottom plate 3. The movable groove 704 is fixedly connected to the inner wall of the side away from the bottom plate 3, and a handle 709 is installed on the side of the end plate 707 away from the lower side plate 703. A longitudinal slide groove 712 is provided inside the plug plate 708, and the top of the longitudinal slide groove 712 passes through the top of the plug plate 708. A block 713 is slidably installed inside the longitudinal slide groove 712, and the top of the block 713 is inserted into the inside of the slot 711. The slot 711 is provided on the inner top wall of the slot 702, and a pressure inclined surface 714 is provided on the top of the block 713. The bottom end of the pressure inclined surface 714 is inclined downward toward the side of the bottom plate 3. A transverse slide groove 715 is provided inside the plug plate 708. The transverse slide groove 715 is located on the side of the longitudinal slide groove 712 away from the deep well plate 1, and the transverse slide groove 715 is close to the longitudinal A connecting groove 716 is provided on one side of the slide groove 712, and a horizontal slide plate 717 is slidably installed inside the horizontal slide groove 715. A connecting rod 718 is provided between the horizontal slide plate 717 and the block 713. The two ends of the connecting rod 718 are respectively hinged to the horizontal slide plate 717 and the block 713. The end of the connecting rod 718 close to the block 713 is tilted downward. A second spring 719 is symmetrically installed on the side of the horizontal slide plate 717 close to the block 713. One end of the second spring 719 is fixedly connected to the inner wall of one end of the horizontal slide groove 715 close to the longitudinal slide groove 712. A push rod 720 is installed on the side of the horizontal slide plate 717 away from the block 713. The push rod 720 penetrates to the side of the insert plate 708 away from the deep hole plate 1. When the insert plate 708 is inserted into the slot 702,The block 713 can be inserted into the slot 711 under the pressure of the pressure-bearing inclined surface 714 and the elastic force of the second spring 719, thereby improving the tight fit stability between the deep-hole plate 1 and the bottom plate 3. At the same time, pushing the push rod 720 can disengage the block 713 from the slot 711, thereby facilitating the disassembly of the deep-hole plate 1 and the bottom plate 3.
[0027] Working principle: When it is necessary to screen strains efficiently, the deep well plate 1 is installed above the bottom plate 3, so that the positioning block 6 is inserted into the positioning groove 5, and the hole groove 2 is located above the containing groove 4;
[0028] When the deep well plate 1 is installed above the bottom plate 3, the insert plate 708 is pulled toward the side away from the bottom plate 3 by the handle 709. When the deep well plate 1 is installed, the insert plate 708 is pushed back so that the insert plate 708 is inserted into the slot 702 on the upper side plate 701, so that the bottom wall of the deep well plate 1 is closely attached to the top wall of the bottom plate 3, so as to prevent the experimental solution from entering other containing tanks 4 through the gap between the deep well plate 1 and the bottom plate 3 during screening, thereby improving the screening effect;
[0029] When the plug plate 708 is inserted into the slot 702, the pressure-bearing inclined surface 714 on the clamping block 713 is pressed downward into the longitudinal slide groove 712. When the clamping block 713 moves to correspond to the clamping groove 711, the clamping block 713 moves upward and is clamped into the clamping groove 711, which facilitates the clamping of the plug plate 708 and improves the tight fit stability between the deep well plate 1 and the bottom plate 3.
[0030] When disassembly is required, hold the handle 709, use the thumb to push the push rod 720 toward the block 713, and then push the block 713 downward through the connecting rod 718 to disengage the card slot 711, so that the plug plate 708 can be moved outward to disassemble the deep well plate 1 and the bottom plate 3;
[0031] Then screening is performed, reactants or compounds under different conditions are added to different wells 2 in the deep well plate 1, and other solutions and impurities after the reaction enter the containing tank 4 through the screening holes, thereby quickly screening out the required Bacillus / Escherichia coli strains.
Claims
1. A highly efficient screening device for Bacillus / Escherichia coli engineered strains, comprising a deep well plate (1), characterized in that: The deep hole plate (1) is evenly provided with hole grooves (2), the bottom wall of the hole grooves (2) is evenly provided with screening holes, a bottom plate (3) is provided below the deep hole plate (1), and holding grooves (4) are evenly provided on the bottom plate (3), the holding grooves (4) correspond to the hole grooves (2) one by one, and the holding grooves (4) are used to hold substances filtered in the hole grooves (2), a clamping fixing component (7) is installed between the deep hole plate (1) and the hole grooves (2), positioning grooves (5) are provided at the four corners of the top end of the bottom plate (3), and positioning blocks (6) are installed at the four corners of the bottom end of the deep hole plate (1), and the positioning blocks (6) are inserted into the interior of the positioning grooves (5); The clamping fixing assembly (7) comprises an upper side plate (701) symmetrically mounted on both sides of the deep-hole plate (1), a slot (702) is provided inside the upper side plate (701), and one end of the slot (702) penetrates to a side of the upper side plate (701) away from the deep-hole plate (1); The clamping and fixing assembly (7) further comprises lower side plates (703) symmetrically mounted on both sides of the hole groove (2), and the lower side plates (703) are located below the upper side plates (701).
2. The device for efficiently screening Bacillus / Escherichia coli engineering strains according to claim 1, characterized in that: The lower side plate (703) is provided with a movable groove (704) inside, and a movable block (705) is slidably installed inside the movable groove (704). A cross bar (706) is installed on the side of the movable block (705) away from the bottom plate (3). The end of the cross bar (706) passes through the side of the lower side plate (703) away from the bottom plate (3). An end plate (707) is fixedly installed on the end of the cross bar (706). An insert plate (708) is fixedly installed on the top of the end plate (707). When the insert plate (708) is inserted into the slot (702), the bottom wall of the deep hole plate (1) is tightly attached to the top wall of the bottom plate (3).
3. The device for efficiently screening Bacillus / Escherichia coli engineering strains according to claim 2, characterized in that: The side of the movable block (705) close to the bottom plate (3) contacts the inner wall of the movable groove (704) close to the bottom plate (3); a first spring (710) is symmetrically mounted on the side of the movable block (705) away from the bottom plate (3); one end of the first spring (710) is fixedly connected to the inner wall of the movable groove (704) away from the bottom plate (3); and a handle (709) is mounted on the side of the end plate (707) away from the lower side plate (703).
4. The device for efficiently screening Bacillus / Escherichia coli engineering strains according to claim 2, characterized in that: The insert plate (708) is provided with a longitudinal slide groove (712) inside, the top end of the longitudinal slide groove (712) extends to the top of the insert plate (708), a clamping block (713) is slidably installed inside the longitudinal slide groove (712), the top end of the clamping block (713) is clamped into the inside of the clamping groove (711), the clamping groove (711) is provided on the inner top wall of the slot (702), a pressure inclined surface (714) is provided at the top end of the clamping block (713), and the bottom end of the pressure inclined surface (714) is inclined downward toward the side of the bottom plate (3).
5. The device for efficiently screening Bacillus / Escherichia coli engineering strains according to claim 4, characterized in that: The insert plate (708) is provided with a transverse slide groove (715) inside. The transverse slide groove (715) is located on the side of the longitudinal slide groove (712) away from the deep hole plate (1). A connecting groove (716) is provided on the side of the transverse slide groove (715) close to the longitudinal slide groove (712). A transverse slide plate (717) is slidably installed inside the transverse slide groove (715). A connecting rod (718) is provided between the transverse slide plate (717) and the block (713). The two ends of the connecting rod (718) are respectively hinged to the transverse slide plate (717) and the block (713). The end of the connecting rod (718) close to the block (713) is tilted downward.
6. The device for efficiently screening Bacillus / Escherichia coli engineering strains according to claim 5, characterized in that: A second spring (719) is symmetrically installed on one side of the transverse slide (717) close to the block (713), one end of the second spring (719) is fixedly connected to the inner wall of one end of the transverse slide groove (715) close to the longitudinal slide groove (712), and a push rod (720) is installed on the side of the transverse slide (717) away from the block (713), and the push rod (720) passes through the side of the insert plate (708) away from the deep hole plate (1).