Cell biological sampler

Abstract

The utility model discloses a cell biological sampler which comprises a supporting table, a placing groove is formed in the upper surface of the supporting table, a placing base is connected to the inner wall of the placing groove in a clamped mode, a cell culture tank is connected to the inner wall of the placing base in a clamped mode, and the upper surface of the cell culture tank is fixedly communicated with a set of sampling pipes. The bottom ends of the group of sampling pipes are respectively positioned at different depth layers of the cell culture tank, a group of extraction bottles are arranged outside the cell culture tank, and the output end of each sampling pipe is fixedly communicated with the upper surface of the extraction bottle. According to the device, the sampling tubes, the suction needle tube, the negative pressure suction tube, the liquid discharge tube and the extraction bottle are arranged, so that cell sap at different positions can be extracted by using the sampling tubes at different depth levels, the sampling tubes and the liquid discharge tube are clamped in sequence through the clamp, the cell sap can be extracted without any contact negative pressure, and sterile sampling of cells is realized; the pollution to the extracted cells is effectively avoided, and the using effect of the cell sampler is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biological cell sampling, in particular to a biological cell sampler. Background Art

[0002] A cell bioreactor is a scientific instrument used in the pharmaceutical field. After cells are stored in a cell bioreactor, various biochemical reactions occur in the bioreactor, thereby affecting the cells through the effects of drugs or other reagents. After the biological reaction, it is necessary to sample the cells stored in the cell bioreactor, and a cell biological sampler is needed for sampling.

[0003] Among them, the utility model patent with publication number CN220224167U discloses a cell bioreactor sampler. However, the above patent cannot achieve aseptic sampling of cells during sampling, which easily causes contamination of cell fluid. In addition, the current sampler has a relatively single fixed position and cannot perform sampling operations on different layers of cell fluid, which reduces the use effect of the cell sampler. Therefore, we propose a cell biological sampler to solve the above problems. Utility Model Content

[0004] The purpose of the utility model is to provide a cell biological sampler to solve the problems raised in the above background technology.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] A cell biological sampler comprises a support platform, the upper surface of the support platform is provided with a placement groove, the inner wall of the placement groove is clamped with a placement base, the inner wall of the placement base is clamped with a cell culture tank, the upper surface of the cell culture tank is fixedly connected with a group of sampling tubes, the bottom ends of a group of sampling tubes are respectively located at different depth layers of the cell culture tank, a group of extraction bottles are arranged outside the cell culture tank, the output end of each sampling tube is fixedly connected with the upper surface of the extraction bottle, the upper surface of each extraction bottle is fixedly connected with a drainage pipe, the upper surface of each extraction bottle is fixedly connected with a negative pressure suction pipe, and the end of each negative pressure suction pipe away from the extraction bottle is fixedly connected with a suction needle tube, the inner bottom wall of the placement groove is fixedly inlaid with a support bearing, the inner ring of the support bearing is fixedly connected with a rotating shaft, and the top end of the rotating shaft is fixedly connected with the bottom surface of the placement base.

[0007] In a further embodiment, a turntable is fixedly connected to the bottom end of the rotating shaft, and a handle is fixedly connected to the bottom surface of the turntable.

[0008] In a further embodiment, two positioning bolts are threadedly connected to the bottom surface of the turntable, and the top end of each positioning bolt is in contact with the bottom surface of the support platform.

[0009] In a further embodiment, a set of scales is provided on the front of the cell culture tank, and the scales are located in the middle of the cell culture tank.

[0010] In a further embodiment, two groups of card slots are provided on the upper surface of the support platform, and the number of the two groups of card slots is at least four.

[0011] In a further embodiment, two groups of supporting legs are fixedly connected to the bottom surface of the support platform, the bottom end of each supporting leg is fixedly connected to a base plate, the bottom surface of each base plate is fixedly connected to a damping pad, and the upper surface of each base plate is provided with two mounting holes.

[0012] Compared with the prior art, the beneficial effects of the utility model are:

[0013] The device is equipped with a sampling tube, a suction needle, a negative pressure suction tube, a drainage tube and an extraction bottle, and can use sampling tubes at different depths to extract cell fluid at different positions. The sampling tube and the drainage tube are clamped in sequence by clamps, and the cell fluid can be extracted by negative pressure without any contact, thereby achieving sterile extraction and sampling of cells, effectively avoiding contamination of cell extraction, and increasing the use effect of this cell sampler. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the overall three-dimensional structure of the cell biological sampler.

[0015] Figure 2 Schematic diagram of the internal anatomical structure of the cell culture tank in the cell biological sampler.

[0016] Figure 3 This is a bottom view of the support table in the cell biological sampler.

[0017] Figure 4 A top view of the support table in the cell biological sampler.

[0018] In the figure: 1. Support table; 2. Card placement slot; 3. Mounting hole; 4. Support leg; 5. Base plate; 6. Placement base; 7. Cell culture tank; 8. Scale; 9. Extraction bottle; 10. Placement groove; 11. Suction needle tube; 12. Negative pressure suction tube; 13. Sampling tube; 14. Drain pipe; 15. Support bearing; 16. Rotating shaft; 17. Turning handle; 18. Positioning bolt; 19. Turntable; 20. Damping pad. DETAILED DESCRIPTION

[0019] In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In addition, the terms "first", "second", etc. are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, features defined as "first", "second", etc. may explicitly or implicitly include one or more of the features. In the description of the present utility model, unless otherwise specified, "multiple" means two or more.

[0020] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood by specific circumstances.

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] See also Figure 1-4In the utility model, a cell biological sampler comprises a support table 1, the upper surface of the support table 1 is provided with a placement groove 10, the inner wall of the placement groove 10 is clamped with a placement base 6, the inner wall of the placement base 6 is clamped with a cell culture tank 7, which is convenient for draining biological cells, and the upper surface of the cell culture tank 7 is fixedly connected with a group of sampling tubes 13, the bottom ends of a group of sampling tubes 13 are respectively located at different depth layers of the cell culture tank 7, which is convenient for sampling operations of cell fluids at different levels, and the outside of the cell culture tank 7 is provided with a group of extraction bottles 9, which is convenient for storing the cell fluid when it is extracted, and the output end of each sampling tube 13 is connected to the extraction bottle 9. The upper surface of the bottle 9 is fixedly connected, and each extraction bottle 9 is fixedly connected with a drainage pipe 14 on its upper surface. Each extraction bottle 9 is fixedly connected with a negative pressure suction pipette 12 on its upper surface. Each negative pressure suction pipette 12 is fixedly connected with a suction needle tube 11 at one end away from the extraction bottle 9, which can perform negative pressure suction on the cell fluid, facilitating the sampling operation of the cell fluid. The inner bottom wall of the placement groove 10 is fixedly inlaid with a support bearing 15, and the inner ring of the support bearing 15 is fixedly connected with a rotating shaft 16. The top end of the rotating shaft 16 is fixedly connected to the bottom surface of the placement base 6, and can rotatably support the placement base 6, facilitating the sampling operation of different cell fluids.

[0023] A turntable 19 is fixedly connected to the bottom end of the rotating shaft 16, and a handle 17 is fixedly connected to the bottom surface of the turntable 19, which can conveniently rotate the placement base 6 to facilitate the rotation adjustment of the cell culture tank 7. Two positioning bolts 18 are threadedly connected to the bottom surface of the turntable 19, and the top of each positioning bolt 18 is in contact with the bottom surface of the support platform 1, which can position the placement base 6 and facilitate stable sampling of biological cells.

[0024] A set of scales 8 are provided on the front of the cell culture tank 7, and the scales 8 are located in the middle of the cell culture tank 7, so that the specific situation of the biological cell fluid inside the cell culture tank 7 can be understood, which is convenient for sampling cells at different levels. Two sets of card placement slots 2 are provided on the upper surface of the support platform 1, and the number of the two sets of card placement slots 2 is at least four, which can place sampling bottles for the convenience of sampling biological cells. Two sets of support legs 4 are fixedly connected to the bottom surface of the support platform 1, and the bottom end of each support leg 4 is fixedly connected to a base plate 5, and the bottom surface of each base plate 5 is fixedly connected to a damping pad 20, and the upper surface of each base plate 5 is provided with two mounting holes 3, which can be installed and fixed to the support platform 1 for the stable sampling of biological cells.

[0025] The working principle of the utility model is:

[0026] When in use, the biological cells are first cultured in the cell culture tank 7. When cell sampling is required, the sampling personnel loosens the positioning bolt 18 according to different sampling requirements, and then turns the handle 17 and the turntable 19 to move the sampling tubes 13 and the extraction bottle 9 at different depths to the extraction position. During the extraction process, the drainage tube 14 is clamped with a clamp, and then the suction needle 11 is pulled to generate negative pressure in the extraction bottle 9. The biological cells are extracted with the sampling tube 13 and flow into the extraction bottle 9. Then, the drainage tube 14 clamp is removed and clamped onto the sampling tube 13, and the suction needle 11 is pushed to discharge the biological cell fluid in the extraction bottle 9 through the drainage tube 14, thereby achieving aseptic sampling of the biological cells.

[0027] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

[0028] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A cell biological sampler, characterized in that: The invention comprises a support platform (1), wherein the upper surface of the support platform (1) is provided with a placement groove (10), the inner wall of the placement groove (10) is clamped with a placement base (6), the inner wall of the placement base (6) is clamped with a cell culture tank (7), the upper surface of the cell culture tank (7) is fixedly connected with a group of sampling tubes (13), the bottom ends of the group of sampling tubes (13) are respectively located at different depth layers of the cell culture tank (7), and the outside of the cell culture tank (7) is provided with a group of extraction bottles (9), and the output end of each sampling tube (13) is connected to the extraction bottle ( 9), the upper surface of each extraction bottle (9) is fixedly connected to a liquid discharge pipe (14), the upper surface of each extraction bottle (9) is fixedly connected to a negative pressure suction pipe (12), and the end of each negative pressure suction pipe (12) away from the extraction bottle (9) is fixedly connected to a suction needle tube (11), the inner bottom wall of the placement groove (10) is fixedly inlaid with a support bearing (15), the inner ring of the support bearing (15) is fixedly connected to a rotating shaft (16), and the top end of the rotating shaft (16) is fixedly connected to the bottom surface of the placement base (6).

2. A cell biological sampler according to claim 1, characterized in that: The bottom end of the rotating shaft (16) is fixedly connected to a rotating disk (19), and the bottom surface of the rotating disk (19) is fixedly connected to a turning handle (17).

3. A cell biological sampler according to claim 2, characterized in that: The bottom surface of the rotating disk (19) is threadedly connected to two positioning bolts (18), and the top end of each positioning bolt (18) is in contact with the bottom surface of the supporting platform (1).

4. A cell biological sampler according to claim 1, characterized in that: A set of scales (8) is provided on the front of the cell culture tank (7), and the scales (8) are located in the middle of the cell culture tank (7).

5. A cell biological sampler according to claim 1, characterized in that: The upper surface of the support platform (1) is provided with two groups of card placement slots (2), and the number of the two groups of card placement slots (2) is at least four.

6. A cell biological sampler according to claim 1, characterized in that: The bottom surface of the support platform (1) is fixedly connected to two groups of support legs (4), and the bottom end of each support leg (4) is A base plate (5) is fixedly connected thereto, and a damping pad (20) is fixedly connected to the bottom surface of each base plate (5). Two mounting holes (3) are provided on the upper surface of each base plate (5).

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