Movable experimental vessel for cell immunofluorescence staining

Abstract

The utility model discloses a movable experimental vessel for cell immunofluorescence staining, which relates to the technical field of cell experimental apparatuses and comprises a bracket and a cover plate placed on the bracket. A plurality of loading openings are formed in the top of the bracket and are arranged at equal intervals; by arranging the inverted circular truncated cone sleeve, the bottom surface of the microscopic cover glass cannot be in direct contact and fit with the bottom surface of the inverted circular truncated cone sleeve, so that the contact force between the microscopic cover glass and the pad is reduced; the trouble that the microscopic cover glass is difficult to take out when being directly attached to the pad plate is avoided; meanwhile, a small amount of antibodies can be added when the antibodies are incubated, and the cell surfaces face downwards, so that the use of the antibodies is saved; the microscopic cover glass can be easily put in and taken out through the triangular cone groove tweezers; the situation that the microscopic cover glass is possibly broken due to frequent taking and putting back is avoided; the movable device enhances independence and convenience of cell experiment operation, and taking and placing operation does not affect cells in other cells of the same batch.

Description

Technical Field

[0001] The utility model relates to the technical field of cell experiment equipment, and more specifically to an active cell immunofluorescence staining experiment vessel. Background Art

[0002] Immunofluorescence technology is a commonly used experimental method in cell and molecular biology. This technology uses fluorescent antibodies (antigens) labeled with fluorescein as probes to detect corresponding antigens (antibodies) in tissues or cells. Fluorescent substances are deposited on the antigen-antibody complex formed in tissues or cells. When the sample is observed under a fluorescence microscope, the fluorescein emits fluorescence when irradiated by the excitation light. The antigen or antibody is located through the cells or tissues where the fluorescence is located. At the same time, the sample can be quantified using quantitative technology. Fluorescent substances can be used to label both antibodies and antigens. However, due to the complex structure and physical and chemical properties of antigens, the conditions for fluorescein labeling are not easy to control. Fluorescent substances are usually used to label antibodies, so it is also called fluorescent antibody technology.

[0003] According to the use of antibodies, immunofluorescence analysis is divided into indirect immunofluorescence analysis and direct immunofluorescence analysis. Indirect immunofluorescence analysis uses an antigen-specific first antibody (primary antibody) to label the antigen, and then uses a second antibody (secondary antibody) coupled with a fluorescent molecule to specifically label the primary antibody, so that the antigen site emits fluorescence. Direct immunofluorescence analysis is a method of directly labeling the antigen with an antigen-specific antibody coupled with a fluorescent molecule. Whether it is indirect immunofluorescence analysis or direct immunofluorescence analysis, the immunofluorescence method based on cell slides is applicable.

[0004] The traditional cell immunofluorescence staining method mainly includes the following steps:

[0005] 1. Plant the cells in a common well plate covered with a microscope cover glass. 2. After the cells grow to the required density, carry out relevant experimental operations and then prepare the cell slides. 3. Aspirate the culture medium, wash the microscope cover glass twice with PBS solution, add fixative to each culture well to fix the cells and then permeabilize the cells (this step can be omitted if only surface proteins are stained). 4. After blocking the cells with blocking solution for a specified time, antibodies can be incubated. 5. Dilute the primary antibody according to the specified multiple with primary antibody diluent, and add the diluted antibody dropwise to the plastic plate covered with sealing film or add the antibody directly to the cell culture plate (the former is complicated to operate and the latter requires a large amount of antibody). 6. If the primary antibody is incubated on the sealing film, it is necessary to use tweezers to clamp the microscope cover glass back to the cell culture plate and wash it with PBST solution. 7. After washing, the slides can be incubated with fluorescently labeled secondary antibodies on plastic plates with sealing film or cell culture plates. Incubate at room temperature in the dark for 1 hour. If the secondary antibodies are incubated on the sealing film, the microscope coverslips need to be clamped back to the cell culture plate with tweezers for washing. Wash with PBST solution in the dark for 3 times, 5 minutes each time. 8. Use the nuclear dye Hoechst33342 in the dark at 1μg / mL to avoid staining the nucleus, and finally wash with PBS solution in the dark for 3 times, 5 minutes each time. 9. Add a drop of anti-fluorescence quenching mounting medium on the slide, and turn the cell side of the antibody-labeled microscope coverslip upside down on the anti-fluorescence quenching mounting medium, and store at 4°C in the dark.

[0006] During the process of cell immunofluorescence staining, it is necessary to put a microscope cover glass in the cell culture plate to culture and process the cells on the microscope cover glass. During the immunofluorescence staining process, it is necessary to put in or take out the microscope cover glass with tweezers many times. Since the bottom of the ordinary cell culture plate is flat, the microscope cover glass is flat, which causes the microscope cover glass to fit tightly in the bottom plane of the round hole of the six-well plate, making it difficult to remove. Even fragments may appear during the operation, causing unnecessary impact on the experimental results. Directly incubating the primary and secondary antibodies in the cell plate requires a large amount of antibodies and high experimental costs; although antibody incubation on the plastic plate of the sealing film saves antibodies, the operation process is cumbersome and time-consuming, and it is also easy to cause problems such as confusion of fragmented samples.

[0007] Based on the above problems, we provide an experimental vessel for active cell immunofluorescence staining.

[0008] The above information disclosed in the background technology is only used to increase the understanding of the background technology of the utility model, therefore, it may include prior art that is not known to ordinary technicians in this field. Utility Model Content

[0009] In order to solve the problems raised in the above background technology, the utility model provides an active cell immunofluorescence staining experimental vessel.

[0010] The utility model provides an active cell immunofluorescence staining experimental vessel using the following technical solutions:

[0011] An active cell immunofluorescence staining experimental vessel comprises a bracket and a cover plate, wherein the cover plate is placed on the bracket; a plurality of loading openings are provided on the top of the bracket, and the loading openings are arranged equidistantly; a plurality of small chambers are sequentially arranged in the corresponding loading openings; wherein the small chambers comprise a straight sleeve, an inverted frustum sleeve and a pad, wherein the inverted frustum sleeve is arranged at the bottom end of the straight sleeve, the pad is arranged at the bottom end of the inverted frustum sleeve, and a triangular cone groove is provided on the inner wall of the inverted frustum sleeve.

[0012] Preferably, a ring sleeve is fixedly connected to the top end of the straight sleeve for contacting the side edge of the loading and releasing opening.

[0013] Preferably, an inclined sleeve is fixedly connected between the ring sleeve and the straight sleeve, and is used to contact the loading and releasing opening to separate the ring sleeve from the bracket.

[0014] Preferably, the top end of the inverted frustum sleeve is fixed with a convex ring, and the inverted frustum sleeve is inserted into the bottom end of the straight sleeve through the convex ring. The outer side surface of the straight sleeve is fixed with a plurality of first elastic strips, and the side surface of the inverted frustum sleeve is fixed with a plurality of first convex columns, and the first elastic strips can be passed through the first convex columns.

[0015] Preferably, a convex ring is fixed to the top of the pad, and the pad is inserted into the bottom end of the inverted round sleeve through the convex ring. A plurality of second elastic strips are fixed to the bottom of the pad, and a plurality of second convex columns are fixed to the side of the inverted round sleeve, and the second elastic strips can be passed through the second convex columns.

[0016] Preferably, the outer edge of the ring sleeve is rounded.

[0017] In summary, the utility model includes the following beneficial technical effects:

[0018] By setting the inverted round table sleeve, the bottom surface of the microscope cover glass will not directly contact and fit with the bottom surface of the inverted round table sleeve, thereby reducing the contact force between the microscope cover glass (other sheet components) and the pad, avoiding the trouble of difficult removal when the microscope cover glass is directly fitted to the pad. At the same time, a small amount of antibody can be added during antibody incubation with the cell side facing down, saving the use of antibodies; the microscope cover glass can be easily put in and out by using triangular pyramid groove tweezers; avoiding the situation that frequent taking out and putting back may cause the microscope cover glass to break; the movable device enhances the independence and convenience of cell experimental operations, and the taking and placing operations do not affect the cells of other chambers in the same batch; at the same time, a place that can be marked is added on the upper edge of the chamber to facilitate marking samples and avoid sample confusion.

[0019] The above summary is for the purpose of description only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments and features described above, further aspects, embodiments and features of the utility model will be readily apparent by reference to the accompanying drawings and the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic structural diagram of an active cell immunofluorescence staining experimental vessel in Embodiment 1 of the present utility model;

[0021] Figure 2 It is a schematic diagram of the structure of the small chamber in the first embodiment of the utility model;

[0022] Figure 3 It is a schematic diagram of the structure of the other side of the small chamber in the first embodiment of the utility model;

[0023] Figure 4 It is a schematic diagram of the internal structure of the small chamber in the first embodiment of the utility model;

[0024] Figure 5 It is a structural schematic diagram of the engaging mechanism in the first embodiment of the utility model;

[0025] Figure 6 This is a schematic cross-sectional view of an active cell immunofluorescence staining experimental vessel in the first embodiment of the present utility model;

[0026] Figure 7 It is a schematic diagram of the structure of the small chamber in the second embodiment of the utility model;

[0027] Figure 8 This is a schematic diagram of the structure of the placement of the microscope cover glass in the first embodiment of the utility model;

[0028] Fig. 9 It is a schematic diagram of the structure of a plurality of triangular pyramidal grooves designed in the first embodiment of the present utility model.

[0029] Explanation of the reference numerals: 1. bracket; 2. cover plate; 3. loading port; 4. small chamber; 400. straight sleeve; 401. frustum sleeve; 402. pad; 403. triangular cone groove; 404. ring sleeve; 405. inclined sleeve; 5. first elastic strip; 6. first convex column; 7. second elastic strip; 8. second convex column. DETAILED DESCRIPTION

[0030] The following is combined with Figures 1 to 6 The utility model is described in further detail.

[0031] It should be noted that the drawings are schematic and not to scale. For the sake of clarity and convenience, the relative sizes and proportions of the parts shown in the drawings are exaggerated or reduced in size, and any size is only illustrative and not restrictive. In addition, the same reference symbol is used for the same structure, element or accessory appearing in more than two figures to reflect similar features.

[0032] Embodiment 1

[0033] The utility model embodiment discloses an active cell immunofluorescence staining experimental vessel. Figures 1 to 6 A movable cell immunofluorescence staining experimental vessel comprises a bracket 1 and a cover plate 2, the cover plate 2 is placed on the bracket 1; a plurality of loading ports 3 are provided on the top of the bracket 1, and the loading ports 3 are arranged at equal intervals; a plurality of small chambers 4 are sequentially arranged in the corresponding loading ports 3; wherein the small chambers 4 comprise a straight sleeve 400, an inverted rounded sleeve 401 and a pad 402, the middle part of the pad 402 is a transparent membrane structure, the inverted rounded sleeve 401 is arranged at the bottom end of the straight sleeve 400, the pad 402 is arranged at the bottom end of the inverted rounded sleeve 401, and the inverted rounded sleeve 401 is provided at the bottom end of the straight sleeve 400. The inner wall is provided with a triangular pyramid groove 403, the diameter of the microscope cover glass is slightly larger than the bottom surface diameter of the inverted cone sleeve 401, and smaller than the upper opening diameter of the inverted cone sleeve 401. When cell culture is performed, the microscope cover glass is placed inside the inverted cone sleeve 401, and the microscope cover glass can be clamped by inserting tweezers into the triangular pyramid groove 403; by setting the inverted cone sleeve 401, the bottom surface of the microscope cover glass will not directly contact and fit with the bottom surface of the inverted cone sleeve 401, thereby reducing the contact force between the microscope cover glass (other sheet-like components) and the pad 402 (such as Figure 8 ), avoiding the trouble of difficult removal when the microscope cover glass is directly attached to the pad 402. At the same time, a small amount of antibody can be added during antibody incubation, and the cells can be turned down to save the use of antibodies; the microscope cover glass can be easily put in and taken out by tweezers through the triangular pyramid groove 403; avoiding the situation that frequent taking out and putting back may cause the microscope cover glass to break; the movable device enhances the independence and convenience of cell experimental operations, and the taking and placing operations do not affect the cells of other chambers in the same batch; at the same time, a place that can be marked is added on the upper edge of the chamber 4 to facilitate marking samples and avoid sample confusion.

[0034] Specifically, the triangular pyramid groove 403 may be provided in plurality (eg Fig. 9 ), by providing a plurality of triangular pyramid grooves 403, it is convenient to take and place the glass slide, and it is also beneficial to clean the chamber 4.

[0035] Specifically, a ring sleeve 404 is fixedly connected to the top end of the straight sleeve 400 for contacting the side edge of the loading opening 3 .

[0036] Specifically, an inclined sleeve 405 is fixedly connected between the ring sleeve 404 and the straight sleeve 400 for contacting the loading port 3 to separate the ring sleeve 404 from the bracket 1. This design allows the ring sleeve 404 to protrude from the bracket 1, making it easier for users to take the small chamber 4.

[0037] Specifically, a convex ring is fixed to the top of the inverted cone sleeve 401, and the inverted cone sleeve 401 is inserted into the bottom end of the straight sleeve 400 through the convex ring. A plurality of first elastic strips 5 are fixed to the outer side surface of the straight sleeve 400, and a plurality of first protruding columns 6 are fixed to the side surface of the inverted cone sleeve 401. The first elastic strips 5 can be passed through the first protruding columns 6. When cleaning the inside of the inverted cone sleeve 401, first, remove the first elastic strips 5 in turn, and then push down the inverted cone sleeve 401, thereby completing the disassembly of the inverted cone sleeve 401, and then clean the inner wall of the inverted cone sleeve 401.

[0038] Specifically, a convex ring is fixed to the top of the pad 402, and the pad 402 is inserted into the bottom end of the inverted rounded sleeve 401 through the convex ring. A plurality of second elastic strips 7 are fixed to the bottom of the pad 402, and a plurality of second protruding columns 8 are fixed to the side of the inverted rounded sleeve 401. The second elastic strips 7 can be passed through the second protruding columns 8. Similarly, the pad 402 can be removed.

[0039] Specifically, the outer edge of the ring sleeve 404 is rounded.

[0040] It is worth noting that the chamber designed in the present application is used for the 24-well plate Transwell aperture in the prior art, that is, the aperture of the loading port 3 in the present application is the aperture of a 24-well plate.

[0041] Reference Figure 7 , both sides of the cover plate 2 are slidably connected with an insertion strip 9, and two positioning holes 11 are provided on the top of the bracket 1, and the insertion strip 9 can be inserted into the positioning holes 11, and the shape of the insertion strip 9 can be designed to be conical, or a magnetic pad is pasted at the bottom end of the insertion strip 9, and an iron sheet is arranged in the positioning hole 11, and a push pad 10 is fixedly connected to the side of the insertion strip 9, and the push pad 10 is arranged on the outside of the cover plate 2, and the insertion strip 9 and the positioning hole 11 together constitute a snap-fit ​​structure. Through this structural design, the stability of the assembly and placement between the cover plate 2 and the bracket 1 can be ensured, and at the same time, it is convenient for the disassembly of the cover plate 2 and the bracket 1.

[0042] First, take the straight sleeve 400 out of the bracket 1, and then pick up the microscope cover glass by inserting tweezers into the triangular cone groove 403; by providing the inverted rounded table sleeve 401, the microscope cover glass can be easily placed therein, and the bottom surface of the microscope cover glass will not directly contact and fit with the bottom surface of the inverted rounded table sleeve 401, thereby reducing the contact force between the microscope cover glass (other sheet-like components) and the pad 402, avoiding the trouble of difficulty in removing the microscope cover glass when it is directly fitted with the pad 402, and also avoiding the situation that frequent removal and replacement may cause the microscope cover glass to break.

[0043] When cleaning the inside of the frustum sleeve 401, first, remove the first elastic strip 5 in sequence, and then push down the frustum sleeve 401, thereby completing the disassembly of the frustum sleeve 401, and then clean the inner wall of the frustum sleeve 401.

[0044] Embodiment 2

[0045] This embodiment is further optimized on the basis of the above embodiment, and the same parts as the above technical solution will not be repeated here. Figure 7 As shown, in order to better realize the present utility model, the following configuration is particularly adopted: In this embodiment, the small chamber 4 is an integrally formed structure and is made of the same material.

[0046] The standard parts used in the utility model can all be purchased from the market, and special-shaped parts can be customized according to the instructions and the drawings. The specific connection methods of each part adopt the conventional means such as mature bolts, rivets, welding, etc. in the existing technology. The machinery, parts and equipment all adopt the conventional models in the existing technology, and the circuit connection adopts the conventional connection method in the existing technology, which will not be described in detail here.

[0047] In the description of the present utility model, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. The meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0048] In the present invention, unless otherwise clearly specified and limited, the terms "install", "connect", "connect", "fix" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0049] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0050] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example" or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, unless they are contradictory.

[0051] In the drawings of the embodiments disclosed by the present invention, only the structures related to the embodiments disclosed by the present invention are involved, and other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of the present invention can be combined with each other.

[0052] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art may still modify the technical solutions described in the aforementioned embodiments, or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. An experimental vessel for active cell immunofluorescence staining, characterized in that: include: A bracket (1) and a cover plate (2), wherein the cover plate (2) is placed on the bracket (1); The top of the bracket (1) is provided with a plurality of loading openings (3), and the loading openings (3) are arranged at equal intervals; A plurality of small chambers (4) are sequentially arranged in the corresponding loading and placing openings (3); The small chamber (4) comprises a straight sleeve (400), an inverted cone sleeve (401) and a pad (402), wherein the inverted cone sleeve (401) is arranged at the bottom end of the straight sleeve (400), the pad (402) is arranged at the bottom end of the inverted cone sleeve (401), and the inner wall of the inverted cone sleeve (401) is provided with a triangular cone groove (403).

2. The experimental vessel for active cell immunofluorescence staining according to claim 1, characterized in that: The top end of the straight sleeve (400) is fixedly connected with a ring sleeve (404) for contacting the side edge of the loading opening (3).

3. The experimental vessel for active cell immunofluorescence staining according to claim 2, characterized in that: An inclined sleeve (405) is fixedly connected between the annular sleeve (404) and the straight sleeve (400) and is used to contact the loading and unloading opening (3) to separate the annular sleeve (404) from the bracket (1).

4. The experimental vessel for active cell immunofluorescence staining according to claim 1, characterized in that: The top end of the inverted round sleeve (401) is fixedly connected with a convex ring, and the inverted round sleeve (401) is plugged into the bottom end of the straight sleeve (400) via the convex ring. The outer side surface of the straight sleeve (400) is fixedly connected with a plurality of first elastic strips (5), and the side surface of the inverted round sleeve (401) is fixedly connected with a plurality of first convex columns (6), and the first elastic strips (5) can be inserted into the first convex columns (6).

5. The experimental vessel for active cell immunofluorescence staining according to claim 1, characterized in that: The top of the pad (402) is fixedly connected with a convex ring, and the pad (402) is inserted into the bottom end of the inverted round table sleeve (401) through the convex ring. The bottom of the pad (402) is fixedly connected with a plurality of second elastic strips (7), and the side of the inverted round table sleeve (401) is fixedly connected with a plurality of second convex columns (8), and the second elastic strips (7) can be inserted into the second convex columns (8).

6. The experimental vessel for active cell immunofluorescence staining according to claim 3, characterized in that: The outer edge of the ring sleeve (404) is rounded.

Images