High performance liquid chromatography analyzer capable of controlling sample introduction of chromatographic columns with different heights

Abstract

A high performance liquid chromatography analyzer capable of controlling sample introduction of chromatographic columns with different heights comprises a high performance liquid chromatography analyzer body, a frame body, an electric proportional valve, a chromatographic column positioning mechanism and a control circuit, the liquid storage tank is mounted on the frame body; a fixing shell is installed on one side of the lower end of the frame body, the lower end of the sample injector is installed in the fixing shell, the side end of an output pipe of the sample injector is connected with one end of an electromagnetic valve, and the other end of the electromagnetic valve is connected with a detector liquid inlet. The chromatographic column positioning mechanism comprises an electric linear sliding table, a connecting pipe and a connecting rod, the liquid pump is mounted on the other side of the lower end of the frame body, a liquid outlet pipe of the electric hydraulic pump is connected with a liquid inlet end of the electric proportional valve, and a liquid outlet end of the electric proportional valve is connected with the connecting pipe through a hose; and the control circuit is arranged in the element box. According to the utility model, chromatographic columns with different heights can be controlled to be compressed in the sample injector by the limiting plate, so that the probability that the chromatographic columns are loosened is reduced, the speed and flow of a mobile phase output into the sample injector can be adjusted, and the detection data of different samples are ensured to be more accurate.

Description

Technical Field

[0001] The utility model relates to the technical field of liquid chromatography analyzer equipment, in particular to a high performance liquid chromatography analyzer capable of controlling sample injection of chromatographic columns of different heights. Background Art

[0002] High performance liquid chromatography analyzer is a device that uses gas chromatography theory to detect and analyze substances based on classical chromatography analyzer. Technically, high performance liquid chromatography analyzer has higher detection sensitivity than ordinary chromatography analyzer and can perform continuous detection of effluent. Specifically, when the high performance liquid chromatograph analyzer is working, its supporting liquid pump sucks the solvent (that is, the mobile phase) in the liquid storage tank and then outputs it into the injector, and the analyte (the analyte, the filler and the liquid isopropanol are pre-pressed to form a chromatographic column) is pre-installed in the supporting injector of the high performance liquid chromatograph analyzer (the injector has an upper cover at the upper end, the upper end of the cover and the liquid outlet pipe of the liquid pump are connected through a hose, the lower end of the injector is welded with a liquid outlet plate with a liquid outlet hole, and the injector is welded with an output pipe on the right side of the lower end of the liquid outlet plate. The lower end of the injector is a closed structure. After the chromatographic column is placed in the injector, the upper cover is covered, and the lower end of the upper cover presses the chromatographic column to prevent the over-pressure mobile phase from dispersing the chromatographic column, causing fine substances in the chromatographic column to enter the detector through the liquid outlet hole at the lower end of the injector, resulting in blockage inside the detector, etc. The mobile phase (the substance that carries the components to be tested forward during the chromatographic column detection process, such as liquid acetonitrile-water solution, acetonitrile-acetic acid aqueous solution, methanol-water solution, acetonitrile-phosphoric acid aqueous solution, etc.) is injected through the liquid inlet tube at the upper end of the injector and passes through the chromatographic column (chromatographic column) located in the injector (injection tube). The effective components in the test object are separated by the chromatographic column and then flow out through the output tube at the lower end of the injector into the detector inside the high performance liquid chromatograph analyzer. The signal detected by the detector is collected and processed by the data processing equipment supporting the high performance liquid chromatograph analyzer, and the chromatogram of the tested substance is recorded, thereby obtaining the composition of the tested substance (the fluid after detection flows out through the waste liquid pipe of the chromatograph analyzer into the waste liquid tank).

[0003] In actual applications of high performance liquid chromatography analyzers, depending on specific detection needs and the amount limit of the object to be measured, there is a problem that the height of the chromatographic column pressed by the chromatographic column pressing equipment is different. In this way, since the height of the injector is fixed, there is a problem that the height of the prepared chromatographic column is too high to be placed in the injector; it is also possible that the height of the prepared chromatographic column is too low, the distance between the upper cover and the lower end of the upper cover is too large, and the higher pressure mobile phase enters the injector and disperses the chromatographic column, causing fine substances in the chromatographic column to enter the detector through the liquid outlet at the lower end of the injector, resulting in blockage inside the detector, etc., which has an adverse effect on the subsequent correct detection and analysis of the object to be measured. In addition, in fact, when different samples of the test objects are tested, the speed and flow rate of the effective substances brought out by the mobile phase flowing through the detector are different, and the test data will be different. For example, some samples require a relatively low speed and flow rate to achieve better detection and analysis results, while some samples require a relatively high speed and flow rate to achieve better detection and analysis results. The existing high-performance liquid chromatography analyzer cannot adjust the speed and flow rate of the liquid mobile phase output by the liquid pump, which will more or less have an adverse effect on the accuracy of the test data. Utility Model Content

[0004] In order to overcome the drawbacks of the existing high performance liquid chromatograph due to structural limitations as described in the background, the utility model provides a high performance liquid chromatograph based on the main body of the high performance liquid chromatograph. Under the joint action of relevant mechanisms in application, after chromatographic columns of different heights are placed in an injector, they can be compressed by a limit plate driven by an electric linear slide, thereby reducing the probability of the chromatographic columns becoming loose, and the mobile phase speed and flow rate output to the injector can be adjusted as needed, thereby ensuring that the detection data of different samples are as accurate as possible. A high performance liquid chromatograph that can control the injection of chromatographic columns of different heights.

[0005] The technical solution adopted by the utility model to solve its technical problems is:

[0006] A high-performance liquid chromatograph capable of controlling the injection of chromatographic columns of different heights, comprising a high-performance liquid chromatograph body, a frame, and an electric proportional valve, characterized in that it also comprises a chromatographic column positioning mechanism and a control circuit; the liquid storage tank of the liquid chromatograph body is installed on the frame; a fixed shell is installed on one side of the lower end of the frame, the lower end of the injector of the liquid chromatograph body is installed in the fixed shell, the output pipe side end of the injector is connected to one end of the electromagnetic valve, and the other end of the electromagnetic valve is connected to the liquid inlet of the detector of the liquid chromatograph; the chromatographic column positioning mechanism comprises an electric linear slide, a connecting pipe, and a connecting rod, and the shell of the electric linear slide is vertically installed on the Between the lower end of the frame and the lower end of the liquid storage tank, one side end of the connecting rod is installed on the side end of the sliding block of the electric linear slide, and the other side end of the connecting rod is installed on the side end of the connecting pipe, a limit plate is installed at the lower end of the connecting pipe, and a vertical pipe is installed at the upper end of the connecting pipe; the liquid pump of the liquid chromatograph analyzer body is installed on the other side of the lower end of the frame, the liquid inlet pipe of the electric hydraulic pump and the liquid outlet end of the liquid storage tank are installed together, the liquid outlet pipe of the electric hydraulic pump is connected to the liquid inlet end of the electric proportional valve, and the liquid outlet end of the electric proportional valve is connected to the vertical pipe via a hose with a length margin; the control circuit is installed in the component box, and the signal output end of the control circuit and the signal input end of the electric proportional valve are electrically connected.

[0007] Preferably, the upper end of the sample injector and the lower end limit plate of the connecting tube are in a vertical plane, and the solenoid valve is a normally closed valve core solenoid valve.

[0008] Preferably, the outer end of the limiting plate has a fixing groove, an elastic sealing ring is installed in the fixing groove, and the outer diameter of the sealing ring is larger than the inner diameter of the injector, the outer diameter of the limiting plate is larger than the outer diameter of the connecting tube, and the position of the limiting plate located inside the connecting tube has multiple liquid inlet holes.

[0009] Preferably, a liquid adding pipe, a liquid adding cover and a one-way air valve are installed at the upper end of the liquid storage tank.

[0010] Preferably, the control circuit comprises an adjustable resistor and a resistor which are electrically connected, and one end of the adjustable resistor is connected to one end of the resistor.

[0011] Compared with the prior art, the present invention has the following beneficial effects: (1) the present invention is based on a high performance liquid chromatograph analyzer body and has all other functions of a common high performance liquid chromatograph analyzer. In application, under the joint action of relevant mechanisms, after chromatographic columns of different heights are placed in the injector, the measuring personnel can control the upward or downward movement of the connecting tube by controlling the working mode of the electric linear slide. In this way, the measuring personnel can conveniently place the chromatographic column in the injector and control the chromatographic columns of different heights to be pressed in the injector by the limiting plate, thereby reducing the probability of the chromatographic column loosening; (2) the measuring personnel can adjust the mobile phase speed and flow rate output to the injector by controlling the circuit and the electric proportional valve, thereby ensuring that the detection data of different samples are more accurate as much as possible. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0013] Figure 1 It is a schematic diagram of the overall structure and the partially enlarged structure of the utility model.

[0014] Figure 2 This is a circuit diagram of the utility model. DETAILED DESCRIPTION

[0015] Figure 1 , 2 As shown, a high-performance liquid chromatograph analyzer capable of controlling the injection of chromatographic columns of different heights comprises a high-performance liquid chromatograph analyzer body (not shown), a power module T1, a frame 1, an electric proportional valve T2, a solenoid valve DC1, power switches T3 and T4, and a chromatographic column positioning mechanism and a control circuit 2; the lower end of the liquid storage tank 3 of the liquid chromatograph analyzer body is welded to the frame 1, and the front end of the frame 1 is an open structure; a fixing shell 4 with a fixing hole in the upper middle part is welded to the left part of the lower end of the frame 1, and the liquid chromatograph analyzer is provided with a plurality of fixing holes on the frame 1. The outer side of the lower end of the sample injector 5 (the inner side height is greater than the highest height of the chromatographic column to be detected and analyzed) of the instrument body is sealed and welded in the fixing hole of the fixed shell 4, and the right end of the output pipe 51 of the sample injector is located outside the middle of the right end of the fixed shell, and the right end of the output pipe 51 is connected to one end of the electromagnetic valve DC1 through a thread, and the other end of the electromagnetic valve DC1 is connected to the upper end of the liquid inlet of the detector (not shown) inside the liquid chromatograph analyzer through a pipeline; the chromatographic column positioning mechanism includes an electric linear slide M1, a connecting pipe 61, and a connecting rod 62, and the electric linear slide M The outer sides of the upper and lower ends of the shell 1 are vertically welded between the middle of the lower end of the frame 1 and the middle of the lower outer end of the liquid storage tank 3, and the sliding block of the electric linear slide M1 is located in the front, the right end of the connecting rod 62 is horizontally welded to the front end of the sliding block, and the left end of the connecting rod 62 is welded to the upper right outer end of the connecting pipe 61. A circular stop plate 63 is welded to the lower end of the connecting pipe 61, and a vertical pipe 7 that communicates with the inside of the connecting pipe 61 is welded to the middle of the upper end of the connecting pipe 61; the electric liquid pump M (280W) of the liquid chromatograph analyzer body is installed on the frame by bolts On the right side of the lower end of the body 1, the liquid inlet pipe of the electric hydraulic pump M and the branch pipe in the middle of the right side of the lower outer end of the liquid storage tank 3 are connected together through a pipe joint, the liquid outlet pipe of the electric hydraulic pump M and the liquid inlet end of the electric proportional valve T2 are threadedly connected, the liquid outlet end of the electric proportional valve T2 and the right end of a horizontal pipe are threadedly connected, and the left end of the horizontal pipe and the upper end of the vertical pipe are connected through a hose 8 with a length margin; the power module T1, the control circuit 2, the power switches T3 and T4 are installed on the circuit board in the plastic box 9, and the plastic box 9 is installed on the left part of the front outer end of the liquid storage tank 3 by bolts.

[0016] Figure 1 , 2As shown, the upper end of the sample injector 5 and the lower end of the connecting tube are located in a vertical plane, and the solenoid valve DC is a normally closed valve core solenoid valve. The outer end of the ring limit plate 63 has an annular fixing groove 64, and an annular rubber ring 65 is tightly sleeved in the fixing groove 64, and the outer diameter of the ring 65 is slightly larger than the inner diameter of the sample injector 5 (injection tube), the outer diameter of the limit plate 63 is larger than the outer diameter of the connecting tube 61, and the surface of the limit plate 63 located inside the connecting tube 61 has multiple liquid inlet holes 631 (to ensure that the mobile phase enters the sample injector). A liquid adding pipe 31 (communicating with the upper end of the liquid storage tank) and a liquid adding cap 32 (open the liquid adding cap 32 to add mobile phase liquid into the liquid storage tank through the liquid adding pipe 31) are installed in the middle of the upper end of the liquid storage tank 3, and a one-way air valve 33 is installed on the right side of the upper end, and the connecting pipe of the one-way air valve 33 is interconnected with the upper end of the liquid storage tank 3 (to ensure that external air can enter the liquid storage tank to prevent vacuum negative pressure from being generated in the liquid storage tank). The control circuit includes an adjustable resistor RP1 and a resistor R1 connected via circuit board wiring, and one end of the adjustable resistor RP1 is connected to one end of the resistor R1. The power input terminals 1 and 2 of the power module T1 are connected to the two poles of the AC 220V through wires, the power input terminal of the electric liquid pump M is connected in series with the power switch T and the two poles of the AC power supply through wires, the power output terminals 3 and 4 of the power module T1 and the power input terminals of the two power switches T3 (the power input terminal of the voltage switch T4), the other end of the adjustable resistor RP1 of the power input terminal of the control circuit and the other end of the resistor R1, and the power input terminals 1 and 2 of the electric proportional valve T2 are connected through wires. One end of the signal output terminal resistor R1 of the control circuit and the signal input terminal 3 of the electric proportional valve T2 are connected through wires, the power output terminals 3, 4 and 5, 6 of the first power switch T3 and the positive and negative or negative and positive power input terminals of the electric linear slide M1 are connected through wires, and the power output terminal of the second power switch T4, the negative power output terminal 4 of the power module T1 and the two ends of the power input of the solenoid valve DC1 are connected through wires.

[0017] Figure 1 , 2 As shown, the new type is based on a high performance liquid chromatography analyzer body 1 and has all the functions of a common high performance liquid chromatography analyzer.

[0018] When the high performance liquid chromatograph analyzer body 1 of the present application is working, its matching liquid pump M is powered on to suck the solvent (that is, the mobile phase) in the liquid storage tank 3, and then outputs it to the injector 5 through the valve core opened by the electric proportional valve T2, the hose 8, the connecting pipe 61 (output through the liquid inlet hole 631 of the connecting pipe), and the measured object (the measured object and the filler and the liquid isopropanol are pre-pressed to form a chromatographic column) in advance. The mobile phase (the substance that carries the component to be measured forward during the detection process of the chromatographic column, such as liquid acetonitrile-water solution, acetonitrile-acetic acid aqueous solution, methanol-water solution, acetonitrile -phosphoric acid aqueous solution, etc.) is injected through the upper end of the sample injector 5, and then passes through the chromatographic column 10 (chromatographic column) located in the sample injector 5 (injection tube). After the effective components in the test object are separated by the mobile phase, they flow out with the mobile phase through the multiple liquid outlet holes of the liquid outlet plate at the lower end of the sample injector 5 and the output tube 51 into the detector inside the high-performance liquid chromatograph analyzer. The signal detected by the detector is collected and processed by the data processing equipment supporting the high-performance liquid chromatograph analyzer, and the chromatogram of the tested substance is recorded, and then the composition of the tested substance is obtained (after detection, the fluid flows out to the waste liquid tank through the waste liquid pipe of the chromatograph analyzer). The multiple liquid outlet holes of the liquid outlet plate ensure that the mobile phase can flow out into the detector, and the liquid outlet plate supports the chromatographic column 10, ensuring that the over-pressure mobile phase will not disperse the chromatographic column 10.

[0019] Figure 1 , 2As shown, after the AC 220V power supply enters the power input terminal of the power module T1, the 3rd and 4th pins of the power module T1 output 12V DC power supply to enter the control circuit, the electric proportional valve T2, and the power input terminal of the power switch. After turning on the power switch T4, the solenoid valve DC1 will be energized and the valve core will open, so that the liquid in the injection tube (injector) can enter the detector; after the power switch T4 is turned off, the solenoid valve DC1 will lose power and the valve core will close, and the liquid in the injection tube will no longer enter the detector. In actual situations, when the staff adjusts the resistance value of the adjustable resistor RP1 to be relatively large or small, the voltage division between it and the resistor R1 will increase or decrease, so that the signal voltage entering the electric proportional valve T2 will decrease or increase, and the internal valve core of the electric proportional valve T2 will open and close relatively small or relatively large, which serves the purpose of adjusting the flow rate and flow rate of the mobile phase liquid entering the injector. In the present application, after the staff turns the handle of the power switch T3 to the left or right, the pins 1, 2 and 3, 4 and 5, 6 of the power switch T3 are connected respectively, so that the positive and negative or negative and positive power input terminals of the electric linear slide M1 will be energized. After the positive and negative power input terminals of the electric linear slide M1 are energized, the sliding block of the electric linear slide M1 (a finished product of an electric screw slide with a power of 100W)) will drive the connecting tube 6 to rise in height and the spacing distance between the upper end of the sampling tube. After it is in place, turn off the power switch T3, and the measurement personnel can put the chromatographic column 10 to be tested into the sampling tube 5 or take it out of the sampling tube 5. After the negative and positive power input terminals of the electric linear slide M1 are energized, the sliding block of the electric linear slide M1 will drive the connecting tube 6 to descend in height, and the lower end of the connecting tube 6 and the limit plate 63 enter the injection tube 5 (the ring 65 can seal between the limit plate and the injection tube to prevent the mobile phase liquid from overflowing upwards). After the lower end of the limit plate 63 presses the chromatographic column, the power switch T3 is turned off; since the limit plate 63 can press the upper end of the chromatographic column, the chance of the chromatographic column loosening is reduced. Figure 2 Among them, the resistance of resistor R1 is 1K; the resistance of adjustable resistor RP1 is 47K; the power supply mechanism T1 is an AC 220V to DC 12V switching power supply module; the solenoid valve DC1 is a 2W normally closed valve core solenoid valve; the power of the electric liquid pump M is 600W; the electric proportional valve T2 is a small electric proportional integral control valve, which has two power input terminals and one signal input terminal. The higher the input signal voltage, the higher the degree of opening of the internal valve core, and vice versa.

[0020] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

Claims

1. A high performance liquid chromatograph capable of controlling the injection of chromatographic columns of different heights, comprising a high performance liquid chromatograph body, a frame, and an electric proportional valve, characterized in that: The invention also comprises a chromatographic column positioning mechanism and a control circuit; the liquid storage tank of the liquid chromatograph analyzer body is installed on the frame; a fixed shell is installed on one side of the lower end of the frame, the lower end of the sample injector of the liquid chromatograph analyzer body is installed in the fixed shell, the output pipe side end of the sample injector is connected to one end of the electromagnetic valve, and the other end of the electromagnetic valve is connected to the liquid inlet of the detector of the liquid chromatograph analyzer; the chromatographic column positioning mechanism comprises an electric linear slide, a connecting pipe and a connecting rod, the shell of the electric linear slide is vertically installed between the lower end of the frame and the lower end of the liquid storage tank, and one side end of the connecting rod is installed on the electric linear slide The sliding block side end of the platform, the other side end of the connecting rod is installed on the side end of the connecting pipe, the lower end of the connecting pipe is installed with a limit plate, and the upper end of the connecting pipe is installed with a vertical pipe; the liquid pump of the liquid chromatograph analyzer body is installed on the other side of the lower end of the frame, the liquid inlet pipe of the electric hydraulic pump and the liquid outlet end of the liquid storage tank are installed together, the liquid outlet pipe of the electric hydraulic pump is connected to the liquid inlet end of the electric proportional valve, and the liquid outlet end of the electric proportional valve is connected to the vertical pipe through a hose with a length margin; the control circuit is installed in the component box, and the signal output end of the control circuit is electrically connected to the signal input end of the electric proportional valve.

2. A high performance liquid chromatography analyzer capable of controlling sample injection into chromatographic columns of different heights according to claim 1, characterized in that: The upper end of the sample injector and the lower end limit plate of the connecting pipe are in a vertical plane, and the solenoid valve is a normally closed valve core solenoid valve.

3. A high performance liquid chromatograph capable of controlling sample injection into chromatographic columns of different heights according to claim 1, characterized in that: The outer end of the limiting plate has a fixing groove, in which an elastic sealing ring is installed, and the outer diameter of the sealing ring is larger than the inner diameter of the injector. The outer diameter of the limiting plate is larger than the outer diameter of the connecting tube. The part of the limiting plate located inside the connecting tube has multiple liquid inlet holes.

4. A high performance liquid chromatography analyzer capable of controlling sample injection into chromatographic columns of different heights according to claim 1, characterized in that: A liquid adding pipe, a liquid adding cover and a one-way air valve are installed on the upper end of the liquid storage tank.

5. The high performance liquid chromatography analyzer capable of controlling sample injection into chromatographic columns of different heights according to claim 1, characterized in that: The control circuit includes an adjustable resistor and a resistor which are electrically connected, and one end of the adjustable resistor is connected to one end of the resistor.

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