Suction filtration device and polypeptide synthesis system

Abstract

The utility model relates to the technical field of biochemical test equipment, and discloses a suction filtration device and a polypeptide synthesis system. The suction filtration device comprises an air inlet pipe, an exhaust pipe, a connecting seat, a three-way valve, a suction filtration bottle and a vacuumizing device. The connecting seat is suitable for mounting and communicating a reaction kettle; the three-way valve is connected among the air inlet pipe, the exhaust pipe and the connecting seat and is suitable for selectively communicating the air inlet pipe or the exhaust pipe with the connecting seat; the exhaust pipe is communicated with the suction flask through a first pipeline; and the vacuumizing device is communicated with the suction flask through a second pipeline. After reaction steps are finished, the vacuumizing device is started, and residual reagents in the reaction kettle can be pumped out under the action of negative pressure, so that reagent residues in the reaction kettle are avoided, the influence of the residues on the next reaction step is prevented, and the purity of synthesized polypeptide can be improved. By utilizing the suction filtration device provided by the embodiment of the invention, the purity effect is improved more obviously in the synthesis process of high-molecular complex polypeptide.

Description

Technical Field

[0001] The utility model relates to the technical field of biochemical test equipment, in particular to a suction filtration device and a polypeptide synthesis system. Background Art

[0002] Peptide is a biologically active substance related to various cell functions in the body. Its molecular structure is between amino acids and proteins. It is a compound composed of multiple amino acids connected by peptide bonds in a certain order. Peptide is a general term for biologically active substances related to various cell functions in the body. It is often used in functional analysis, antibody research, and especially drug development.

[0003] In the field of peptide synthesis, nitrogen is usually used to pass through the reactor to allow the reagents and resin to fully react in the reactor. After the reaction is completed, the nitrogen is turned off and the reagents in the reactor automatically flow out.

[0004] However, in actual production, a large amount of reagents from the previous step reaction usually remain in the reactor, resulting in a large amount of impurities when other reagents are added in the second step, which greatly reduces the purity of the synthesized polypeptide. Therefore, removing the residual reagents in the resin after the reaction has become the main direction of research and development in the polypeptide field. Utility Model Content

[0005] In view of this, the utility model provides a suction filtration device and a polypeptide synthesis system to solve the problem that residual reagents in the reaction kettle lead to reduced purity of synthesized polypeptides.

[0006] In the first aspect, the utility model provides a suction filtration device, comprising an air inlet pipe, an air extraction pipe, a connecting seat, a three-way valve, a suction filtration bottle and a vacuum device. The connecting seat is suitable for installing and connecting a reaction kettle; the three-way valve is connected between the air inlet pipe, the air extraction pipe and the connecting seat, and the three-way valve is suitable for selectively connecting the air inlet pipe or the air extraction pipe to the connecting seat; the air extraction pipe is connected to the suction filtration bottle through a first pipeline; the vacuum device is connected to the suction filtration bottle through a second pipeline.

[0007] Beneficial effect: By setting the air inlet pipe and the air extraction pipe, when performing the polypeptide synthesis reaction, the three-way valve is switched to connect the air inlet pipe with the connection seat, that is, when the reactor is connected to the connection seat, the air inlet pipe is connected to the reactor, so that an inert gas, such as nitrogen, can be introduced into the reactor through the air inlet pipe for the reagent and the resin to fully react. After waiting for the reaction to end, the inert gas source is turned off to stop the air intake of the air inlet pipe. The three-way valve is switched to connect the air extraction pipe with the connection seat, that is, when the reactor is connected to the connection seat, the air extraction pipe is connected to the reactor, and the reagent in the reactor automatically flows out from the discharge port of the reactor. Then the filtration operation is performed. Since the reactor is connected to the vacuum device through the air extraction pipe, the first pipeline, the suction bottle, the second pipeline, and the vacuum device, the vacuum device is started, and the residual reagent in the reactor can be extracted under the action of negative pressure, thereby avoiding the residual reagent in the reactor and preventing the residual from actually affecting the next reaction step, thereby improving the purity of the synthesized polypeptide. For high molecular complex polypeptides, multiple reactions are usually required. By using the suction filtration device provided in the embodiment of the utility model, suction filtration can be performed after each reaction, thereby ensuring the synthesis purity of the high molecular polypeptide. By using the suction filtration device provided in this embodiment, the purity effect is more significantly improved during the synthesis of high molecular complex polypeptides.

[0008] In an optional embodiment, an air inlet is provided at one end of the air inlet pipe, and the other end of the air inlet pipe is closed, and the air inlet is suitable for connecting to an inert gas source; one end of the exhaust pipe is closed, and the other end is provided with an air outlet, and the air outlet is connected to one end of the first pipeline, and the other end of the first pipeline is connected to the suction bottle.

[0009] In an optional embodiment, there are multiple connecting seats, the number of three-way valves is set corresponding to the connecting seats, and the opening of any three-way valve is suitable for connecting the air inlet pipe or the air exhaust pipe to the connecting seat selectively.

[0010] In an optional embodiment, a water tank is further included, and the outlet of the vacuum device is connected to the water tank.

[0011] In an optional embodiment, the water tank is provided with a water inlet, a drain outlet, a circulation inlet and a circulation outlet, the circulation inlet and the circulation outlet are connected via a water circulation pipeline, the water circulation pipeline is connected to a circulation water pump, and the outlet of the vacuum device is connected to the water circulation pipeline.

[0012] In an optional embodiment, the vacuum extraction device includes a vacuum pump, and the vacuum pump is connected to the suction filtration bottle through a second pipeline.

[0013] In an optional embodiment, the air inlet pipe and the air exhaust pipe are arranged on the same horizontal plane, and the connecting seat is vertically arranged above the air inlet pipe or the air exhaust pipe.

[0014] In an optional embodiment, the first port of the three-way valve is controllably connected to the air intake pipe through a first branch, the second port of the three-way valve is controllably connected to the air exhaust pipe through a second branch, the third port of the three-way valve is set and connected to the connecting seat, and the first branch and the second branch are not colinear.

[0015] In a second aspect, the utility model further provides a polypeptide synthesis system, comprising a reaction kettle and a suction filtration device according to any one of the above technical solutions. The reaction kettle is detachably connected to a connection seat of the suction filtration device.

[0016] Beneficial effects: Since the polypeptide synthesis system includes a suction filtration device, it has the same effect as the suction filtration device and will not be described in detail here.

[0017] In an optional embodiment, a sand filter layer is provided in the reactor. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the specific implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 This is a schematic structural diagram of a suction filtration device according to an embodiment of the utility model;

[0020] Figure 2 The figure is a schematic diagram of the partial structure of a polypeptide synthesis system according to an embodiment of the present utility model.

[0021] Description of reference numerals:

[0022] 1. Air inlet pipe; 2. Air exhaust pipe; 3. Connecting seat; 4. Three-way valve; 5. Filter bottle; 6. Vacuum pump; 7. Water tank; 701. Water inlet; 702. Drain outlet; 8. Water circulation pipeline; 9. Circulating water pump; 10. First pipeline; 11. Second pipeline; 12. First branch; 13. Second branch; 20. Reactor. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical solution and advantages of the embodiment of the utility model clearer, the technical solution in the embodiment of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiment of the utility model. Obviously, the described embodiment is a part of the embodiment of the utility model, not all the embodiments. Based on the embodiment of the utility model, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the utility model.

[0024] Combine the following Figure 1 to Figure 2 , describing an embodiment of the utility model.

[0025] According to an embodiment of the utility model, on the one hand, a suction filtration device is provided, comprising an air inlet pipe 1, an air extraction pipe 2, a connecting seat 3, a three-way valve 4, a suction filtration bottle 5 and a vacuum device. The connecting seat 3 is suitable for installing and connecting the reaction kettle 20; the three-way valve 4 is connected between the air inlet pipe 1, the air extraction pipe 2 and the connecting seat 3, and the three-way valve 4 is suitable for selectively connecting the air inlet pipe 1 or the air extraction pipe 2 to the connecting seat 3; the air extraction pipe 2 is connected to the suction filtration bottle 5 through the first pipeline 10; the vacuum device is connected to the suction filtration bottle 5 through the second pipeline 11.

[0026] In a peptide synthesis system, the reactor 20 is installed in the connection seat 3, and the inner cavity of the reactor 20 is connected to the connection seat 3. When adding reagents, the three-way valve 4 is operated to connect the air inlet pipe 1 and the reactor 20, so that the inert gas source passes through the three-way valve 4 to reach the reactor 20, and the reagents and the resin are fully reacted through the inert gas source.

[0027] By setting the air inlet pipe 1 and the air extraction pipe 2, when performing the polypeptide synthesis reaction, switch the three-way valve 4 to connect the air inlet pipe 1 with the connecting seat 3, that is, when the reactor 20 is connected to the connecting seat 3, connect the air inlet pipe 1 with the reactor 20, so that an inert gas, such as nitrogen, can be introduced into the reactor 20 through the air inlet pipe 1 for the reagent and the resin to react fully. After waiting for the reaction to end, turn off the inert gas source and stop the air intake of the air inlet pipe 1. Switch the three-way valve 4 to connect the air extraction pipe 2 with the connecting seat 3, that is, when the reactor 20 is connected to the connecting seat 3, connect the air extraction pipe 2 with the reactor 20, and the reagent in the reactor 20 automatically flows out from the discharge port of the reactor 20. Then perform the suction filtration operation. Since the reactor 20 is connected to the vacuum device through the exhaust pipe 2, the first pipeline 10, the suction bottle 5, the second pipeline 11, and the vacuum device is started, the residual reagents in the reactor 20 can be extracted under the action of negative pressure, thereby avoiding the residual reagents in the reactor 20 and preventing the residual from actually affecting the next reaction step, thereby improving the purity of the synthesized polypeptide. For high-molecular complex polypeptides, multiple reactions are usually required. By using the suction filtration device provided in the embodiment of the utility model, suction filtration can be performed after each reaction, thereby ensuring the purity of the high-molecular polypeptide synthesis. By using the suction filtration device provided in this embodiment, the purity effect can be improved more significantly during the synthesis of high-molecular complex polypeptides.

[0028] In some embodiments, an air inlet is provided at one end of the air inlet pipe 1, and the other end of the air inlet pipe 1 is closed, and the air inlet is suitable for connecting to an inert gas source; one end of the exhaust pipe 2 is closed, and the other end is provided with an air outlet, and the air outlet is connected to one end of the first pipeline 10, and the other end of the first pipeline 10 is connected to the suction bottle 5.

[0029] Since an air inlet is provided at one end of the air inlet pipe 1, an inert gas source can be connected to deliver the gas into the reactor 20. The other end of the air inlet pipe 1 is closed to ensure that all the gas enters the reactor 20. An air outlet is provided at one end of the air extraction pipe 2, and the air extraction pipe 2 and the suction bottle 5 can be connected through the first pipeline 10. One end of the air extraction pipe 2 is closed to ensure that all the gas containing the reagent extracted enters the suction bottle 5, thereby ensuring the effect of suction filtration.

[0030] In some embodiments, there are multiple connecting seats 3, and the number of three-way valves 4 is set corresponding to the connecting seats 3. The opening of any three-way valve 4 is suitable for connecting the air inlet pipe 1 or the air exhaust pipe 2 to the connecting seat 3 selectively.

[0031] A plurality of connection seats 3 are provided, and correspondingly, a plurality of three-way valves 4 are also provided, so that each three-way valve 4 controls the connection and disconnection between each connection seat 3 and the air inlet pipe 1 or the air extraction pipe 2. A plurality of connection seats 3 and three-way valves 4 are provided, and a plurality of connection seats 3 can be installed with a plurality of reaction kettles 20 at the same time, so that a plurality of reaction kettles 20 can react or extract at the same time, thereby improving the operation efficiency.

[0032] By controlling the three-way valve 4, the connection between the air inlet pipe 1, the air extraction pipe 2 and the connecting seat 3 can be controlled, and the size of the air flow can also be controlled.

[0033] In some embodiments, a water tank 7 is further included, and the outlet of the vacuum device is connected to the water tank 7 .

[0034] By setting up the water tank 7 and connecting the outlet of the vacuum device to the water tank 7, the residual reagents and waste gas filtered out are adsorbed into the water in the water tank 7, so that the waste water can be discharged to the wastewater treatment facility through the drainage system to prevent air pollution.

[0035] In some embodiments, the water tank 7 is provided with a water inlet 701, a drain outlet 702, a circulation inlet and a circulation outlet. The circulation inlet and the circulation outlet are connected through a water circulation pipeline 8. The water circulation pipeline 8 is connected to a circulation water pump 9. The outlet of the vacuum device is connected to the water circulation pipeline 8.

[0036] The water inlet 701 and the drain outlet 702 of the water tank 7 can be used to replace the water in the water tank 7 and discharge the waste water. The circulation inlet and the circulation outlet of the water tank 7 are connected through the water circulation pipeline 8. Under the power of the circulating water pump 9, the water circulation in the water tank 7 can be realized, and the outlet of the vacuum device is connected to the water circulation pipeline 8. The residual reagents and waste gas filtered by vacuum enter the water circulation pipeline 8, participate in the water circulation, and can be thoroughly mixed with water and discharged.

[0037] Specifically, after a reaction process of polypeptide synthesis is completed, the three-way valve 4 is adjusted, the air extraction pipe 2 is connected to the reactor 20, and the reactor 20 is in a suction filtration working state. After the reagent flows into the suction filtration bottle 5 through the air extraction pipe 2, the vacuum negative pressure provided by the vacuum pump 6 machine room causes the air to flow from the reactor 20 to the suction filtration bottle 5, and finally the air and waste gas with the reagent residue are sucked into the water circulation pipeline 8. The residual reagent and waste gas in the air are adsorbed into the water in the water tank 7 through the water circulation pipeline 8, and then the waste water is discharged to the wastewater treatment facility through the drainage system.

[0038] In some embodiments, the vacuum pump includes a vacuum pump 6 , which is connected to the suction flask 5 via a second pipeline 11 .

[0039] Specifically, the vacuum pump 6 provides vacuum negative pressure adsorption force to extract the residual reagents and waste gas in the reaction kettle 20, so as to prevent the residual reagents from affecting the subsequent reaction steps and improve the purity of the polypeptide synthesis.

[0040] In some embodiments, the air inlet pipe 1 and the air exhaust pipe 2 are arranged on the same horizontal plane, and the connecting seat 3 is vertically arranged above the air inlet pipe 1 or the air exhaust pipe 2 .

[0041] The connection seat 3 is arranged vertically, so that the reactor 20 can be vertically installed on the connection seat 3. The connection seat 3 is arranged vertically above the air inlet pipe 1 or the air extraction pipe 2, and the reactor 20 is arranged above the air inlet pipe 1 or the air extraction pipe 2. In this way, in the reaction state, the nitrogen in the air inlet pipe 1 enters the reactor 20 from bottom to top, and the solution in the reactor 20 is located at the bottom under the action of gravity. The nitrogen will enter the solution and generate bubbles, which is conducive to the full reaction of the solution reagent. In the filtration state, the residual reagent in the reactor 20 will flow downward to the air extraction pipe 2 below the reactor 20 under the action of pressure and gravity. Combined with the vacuuming effect, the residual reagent and waste gas in the reactor 20 can be completely filtered out.

[0042] In some embodiments, the first port of the three-way valve 4 is controllably connected to the intake pipe 1 through the first branch 12, the second port of the three-way valve 4 is controllably connected to the exhaust pipe 2 through the second branch 13, the third port of the three-way valve 4 is set and connected to the connecting seat 3, and the first branch 12 and the second branch 13 are not colinear.

[0043] Since the first branch 12 and the second branch 13 are not collinear, in one embodiment, the first branch 12 and the second branch 13 form a "Z"-shaped structure, forming a circuitous structure, which can prevent accidental connection between the air inlet pipe 1 and the air exhaust pipe 2, prevent nitrogen from blowing the reaction products away from the reactor 20, and ensure the filtration effect.

[0044] The suction filtration device provided by the utility model has the following advantages:

[0045] 1. The air inlet pipe 1 or the air extraction pipe 2 is selectively connected to the connection seat 3 through the three-way valve 4, and the reaction kettle 20 can be filtered after the reaction is completed to improve the efficiency of synthesis and the purity of the product. The production advantages are more obvious especially for high molecular weight and complex polypeptides (which need to be repeated many times).

[0046] 2. Using a vacuum device for filtration can not only extract the residual reagents, but also significantly reduce the emission of organic waste gas in the reactor 20.

[0047] According to an embodiment of the present invention, on the other hand, a polypeptide synthesis system is provided, comprising a reaction kettle 20 and a suction filtration device according to any one of the above technical solutions. The reaction kettle 20 is detachably connected to the connection seat 3 of the suction filtration device.

[0048] Because the polypeptide synthesis system includes a suction filtration device, which has the same effect as the suction filtration device, it will not be described in detail here.

[0049] In some embodiments, a sand filter layer is provided in the reactor 20 .

[0050] Nitrogen is introduced into the reactor 20 through the air inlet pipe 1. The force provided by the nitrogen prevents the reagent solution from leaking from the sand filter layer in the reactor 20. The nitrogen is introduced into the reagent solution from bottom to top, causing the solution to produce physical reactions such as bubbles, thereby allowing the liquid reagent and resin in the reactor 20 to fully react.

[0051] When the connection seat 3 and the suction pipe 2 are interconnected, due to pressure reasons and the gravity of the solution, the sand filter layer in the reagent solution reactor 20 gradually flows into the suction pipe 2 below the connection seat 3, and is collected in the suction bottle 5 through the first pipeline 10. After all the solution reagents flow to the suction pipe 2 below, the residual solution of the solid resin on the sand filter layer is further sucked away by the negative pressure of vacuum extraction.

[0052] Although the embodiments of the present invention are described in conjunction with the accompanying drawings, those skilled in the art may make various modifications and variations without departing from the spirit and scope of the present invention, and such modifications and variations are all within the scope defined by the appended claims.

Claims

1. A suction filtration device, characterized in that: include: Intake pipe (1); Air extraction pipe (2); A connecting seat (3), wherein the connecting seat (3) is suitable for installing and connecting to a reaction kettle (20); A three-way valve (4), the three-way valve (4) being connected between the air inlet pipe (1), the air extraction pipe (2) and the connecting seat (3), the three-way valve (4) being suitable for selectively connecting the air inlet pipe (1) or the air extraction pipe (2) to the connecting seat (3); A suction filtration bottle (5), wherein the suction pipe (2) is connected to the suction filtration bottle (5) via a first pipeline (10); A vacuum pumping device is connected to the suction filtration bottle (5) via a second pipeline (11).

2. The filtration device according to claim 1, characterized in that: One end of the air inlet pipe (1) is provided with an air inlet, the other end of the air inlet pipe (1) is closed, and the air inlet is suitable for connecting to an inert gas source; One end of the air extraction pipe (2) is closed, and the other end is provided with an air outlet, the air outlet is connected to one end of the first pipeline (10), and the other end of the first pipeline (10) is connected to the suction filtration bottle (5).

3. The suction filtration device according to claim 1 or 2, characterized in that: The connecting seat (3) is provided with a plurality of them, the number of the three-way valves (4) is arranged corresponding to the connecting seat (3), and the opening of any one of the three-way valves (4) is suitable for selectively connecting the air intake pipe (1) or the air exhaust pipe (2) with the connecting seat (3).

4. The suction filtration device according to claim 1 or 2, characterized in that: It also comprises a water tank (7), and the outlet of the vacuum extraction device is in communication with the water tank (7).

5. The filtration device according to claim 4, characterized in that: The water tank (7) is provided with a water inlet (701), a water outlet (702), a circulation inlet and a circulation outlet; the circulation inlet and the circulation outlet are connected via a water circulation pipeline (8); the water circulation pipeline (8) is connected to a circulation water pump (9); and the outlet of the vacuum device is connected to the water circulation pipeline (8).

6. The suction filtration device according to claim 1 or 2, characterized in that: The vacuum extraction device comprises a vacuum pump (6), and the vacuum pump (6) is connected to the suction filtration bottle (5) through the second pipeline (11).

7. The suction filtration device according to claim 1 or 2, characterized in that: The air intake pipe (1) and the air exhaust pipe (2) are arranged on the same horizontal plane, and the connecting seat (3) is arranged vertically above the air intake pipe (1) or the air exhaust pipe (2).

8. The suction filtration device according to claim 1 or 2, characterized in that: The first port of the three-way valve (4) is controllably connected to the air intake pipe (1) via a first branch (12), the second port of the three-way valve (4) is controllably connected to the air extraction pipe (2) via a second branch (13), the third port of the three-way valve (4) is disposed and connected to the connecting seat (3), and the first branch (12) and the second branch (13) are not collinear.

9. A polypeptide synthesis system, characterized in that: include: Reactor (20); The suction filtration device according to any one of claims 1 to 8, wherein the reaction kettle (20) is detachably connected to the connecting seat (3) of the suction filtration device.

10. The polypeptide synthesis system according to claim 9, characterized in that: A sand filter layer is provided in the reactor (20).

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