Biological safety protection material supplementing device
By designing a biosafety protective feeding device, the air purification structure and purified air storage room are used to achieve automatic sterilization of air, which solves the problem of susceptibility to pollution in the fermentation process and improves the safety and efficiency of the production process.
Patent Information
- Application Number
- CN202421230545.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-31
- Publication Date
- 2025-03-04
- Estimated Expiration
- 2034-05-31
AI Technical Summary
During the fermentation process, feed bottles or feed tanks are prone to miscellaneous bacterial contamination and infection with phages or other pathogens, resulting in huge safety hazards during the production process.
Design a biosafety protective feeding device, including a feed tank, an air inlet, an outlet, an air purification structure and a purified air storage room. The incoming air is processed through the air purification structure, and the purified air is stored for ventilation in the storage chamber to achieve automatic sterilization and avoid contamination of the material culture medium.
It effectively prevents phage and other microorganisms during bio-fermentation experiments and fermentation production processes, improves the safety and efficiency of the production process, and reduces the risk of miscellaneous bacterial contamination and pathogen infection.
Smart Images
Figure CN222556930U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of microbial culture and fermentation engineering, and in particular to a biosafety protection feeding device for protecting materials infected by viruses such as microorganisms and bacteriophages during culture and fermentation. Background Art
[0002] Fermentation engineering, as an important application field of synthetic biology technology, refers to the process of converting organic matter into products with economic value through the metabolic activities of microorganisms. Driven by synthetic biology, fermentation technology has developed rapidly, covering a wide range of fields, including food, medicine, energy, environmental protection and other aspects. At present, the fermentation industry has formed a complete industrial chain, covering multiple links such as raw material supply, fermentation process control, product extraction and purification. Globally, the fermentation industry has shown strong development momentum in the fields of food, beverages, feed, biopesticides, bioenergy, etc. However, during the fermentation process, bacterial and viral contamination may lead to serious consequences. First, bacterial contamination may lead to the loss of control of the fermentation process and make the fermentation process unable to proceed normally. Secondly, viral contamination should not be ignored. Viruses are microorganisms that parasitize living cells. They can destroy the integrity of cells by infecting target microorganisms in the fermentation process, causing cell death or metabolic abnormalities. This will not only reduce the yield of fermentation products and make industrial production impossible, but also may cause the products to carry viruses, posing a threat to human health. Therefore, it is crucial to prevent bacterial and viral contamination during the fermentation process. This needs to be achieved through strict operating procedures, high-quality raw materials, good equipment maintenance, regular testing and monitoring, etc. More importantly, experimental or production equipment with protection capabilities is very necessary. Among them, feeding equipment plays an important role in the fermentation production process. Therefore, it is necessary to develop a protective feeding device that can achieve biosafety protection. Utility Model Content
[0003] In order to solve the problem that the feeding bottles or feeding tanks in the prior art are prone to contamination by miscellaneous bacteria and infection by bacteriophages or other pathogens during experiments or fermentation production, resulting in huge safety hazards in the production process that are difficult to deal with, the utility model provides a biosafety protection feeding device to solve the problem that the infection of feeding bottles in current laboratories and fermentation production processes seriously affects production efficiency.
[0004] The utility model relates to a biosafety protection feeding device, comprising a feeding tank, an air inlet and a discharge port arranged on the feeding tank, a storage chamber connected with the discharge port and used for storing materials is arranged in the feeding tank, an air purification structure and a purified air storage chamber are also arranged in the feeding tank, and a liquid-stopping structure is arranged between the air purification structure and the purified air storage chamber, wherein one end of the air purification structure is communicated with the air inlet, and the other end is communicated with one end of the purified air storage chamber, and the other end of the purified air storage chamber is communicated with the air circulation port above the storage chamber through a breathing switch, and external air enters the feeding tank through the air inlet, and after being purified by the air purification structure, enters the purified air storage chamber for storage, so as to provide ventilation in the storage chamber.
[0005] Furthermore, a top cover is provided on the top of the feeding tank, and the discharge port is arranged on the top cover.
[0006] Furthermore, the top cover is also provided with a discharge pipe connected with the discharge port, and the discharge pipe or the discharge port of the top cover is provided with a discharge switch.
[0007] Furthermore, the feeding tank includes a cylindrical first tank body and a conical second tank body arranged above the cylindrical first tank body and integrally arranged with the cylindrical first tank body, and the top cover is arranged on the top surface of the conical second tank body.
[0008] Furthermore, a stirring paddle is provided in the storage chamber, and a driving device for driving the stirring paddle is also provided on the feeding tank.
[0009] Furthermore, the feed replenishment tank is also provided with a heating device for heating the material in the storage chamber.
[0010] Furthermore, the air inlet is arranged above one side of the outside of the storage chamber, and the purified air storage chamber and the air circulation port are arranged on the other side of the storage chamber, wherein the air circulation port is arranged on the upper side wall of the storage chamber.
[0011] Furthermore, a first air channel is provided at the other end of the purified air storage chamber, the air circulation port is communicated with the purified air storage chamber through the first air channel, and the air switch is arranged at the end of the first air channel close to the air circulation port.
[0012] Furthermore, the air purification structure is a tank bottom space arranged at the bottom of the feeding tank and below the storage chamber, the air inlet is connected to the tank bottom space through a second air channel, the tank bottom space is used to fill with sterilization and disinfection liquid, and the volume of the purified air storage chamber is 3-10 times the volume of the second air channel.
[0013] Furthermore, a plurality of load-bearing brackets for supporting the storage chamber are provided at the bottom of the feeding tank.
[0014] Compared with the prior art, the utility model has the following beneficial effects: simple structure, reasonable design. The air entering the device (feeding bottle or feeding tank) is treated by the air purification structure, which is safe and reliable, and can effectively prevent bacteriophage and other microbial contamination in biological fermentation experiments and fermentation production processes.
[0015] When in use, add culture medium into the storage chamber, then close the breathing switch first, close the external air passage, seal the storage chamber, and sterilize the entire feeding tank with high temperature and high pressure. This step is the same as the existing sterilization step. The advantage of the utility model is that after sterilization, the air purification structure and the purified air storage chamber are isolated, and sterilization and disinfection liquid is added to the air purification structure. During the fermentation process, turn on the air switch and the liquid stop structure switch to automatically sterilize the air entering the storage chamber to avoid contamination of the material culture medium.
[0016] This device is easy to operate, saves time and effort, is safe and efficient, and ensures quality and quantity. It can meet the needs of both large-scale microbial fermentation manufacturers and small-scale producers, and can also be widely used in fermentation engineering experiments. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] In order to more clearly illustrate the solutions in the present invention or the prior art, a brief introduction is given below to the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.
[0018] Figure 1 It is a schematic diagram of the overall structure of the utility model;
[0019] Figure 2 It is a schematic diagram of the internal structure of an embodiment of the utility model. DETAILED DESCRIPTION
[0020] Unless otherwise defined, all technical and scientific terms used in the present invention have the same meanings as those commonly understood by technicians in the technical field of the present invention; the terms used in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention; the terms "including" and "having" and any variations thereof in the specification and claims of the present invention and the above-mentioned drawings are intended to cover non-exclusive inclusions. The terms "first", "second", etc. in the specification and claims of the present invention or the above-mentioned drawings are used to distinguish different objects, not to describe a specific order.
[0021] Reference to "embodiments" in the present invention means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present invention. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it mutually exclusive, independent, or alternative to other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described in the present invention may be combined with other embodiments.
[0022] In order to enable those skilled in the art to better understand the solution of the utility model, the technical solution in the embodiment of the utility model will be clearly and completely described below in conjunction with the accompanying drawings.
[0023] like Figure 1 As shown, the utility model includes a feeding tank 100, an air inlet 2 and a discharge port 110 arranged on the feeding tank 100, a storage chamber connected to the discharge port 110 and used to store materials is provided in the feeding tank 100, and a biosafety protection system capable of entering the storage chamber is also provided in the feeding tank 100. The biosafety protection system of this example is located outside the storage chamber, and the biosafety protection system of this example is integrated into the feeding tank and is self-contained with the feeding tank. A breathing switch 9 and a liquid-stopping structure 6 are also provided on the feeding tank, wherein the breathing switch is used for switching the storage chamber and the external safety protection system, and the liquid-stopping structure 6 is used for isolating the air purification, sterilization and disinfection liquid in the safety protection system from the purified air.
[0024] As a preferred embodiment of the utility model, the feeding tank 100 of this example includes a cylindrical first tank body 101 and a conical second tank body 102 arranged above the cylindrical first tank body 101 and integrally arranged with the cylindrical first tank body 101. A top cover 11 is provided on the top surface of the conical second tank body 102, and the top cover 11 can be opened to realize feeding into the storage chamber. The top of the feeding tank 100 is set to a cone, which effectively reduces the top surface area of the storage tank 100, reduces the contact area between the material in the storage chamber and the external air, and reduces the risk of infection. The discharge port 110 of this example is set on the top cover 11, and the discharge can be realized through a connected pipe and a pump. Of course, this example can also adopt other feeding bottle structures, such as a cone, a cylinder, a test tube, etc.
[0025] like Figure 1 and Figure 2 As shown, a discharge pipe 10 can also be provided on the discharge port 110 of this example, and then a discharge valve can be provided on the discharge pipe to achieve isolation from the outside during the fermentation process. Of course, the discharge valve can also be provided on the discharge port, or the discharge pipe 10 can be externally connected to a control mechanism such as a water pump.
[0026] As a preferred embodiment of the present utility model, the feeding tank 100 of this example is also provided with an air purification structure 4 and a purified air storage chamber 8, and a liquid-stopping structure 6 arranged between the air purification structure 4 and the purified air storage chamber 8, wherein one end of the air purification structure 4 is connected to the air inlet 2, and the other end is connected to one end of the purified air storage chamber 7, and the other end of the purified air storage chamber 7 is connected to the air circulation port above the storage chamber 1 through a breathing switch 9, and the external air enters the feeding tank 100 through the air inlet 2, and after being purified by the air purification structure 4, enters the purified air storage chamber 7 for storage for ventilation in the storage chamber 1.
[0027] The air inlet 2 of this example is arranged above the outer side of the storage chamber 1, and the purified air storage chamber 7 and the air circulation port are arranged on the other side of the storage chamber 1, wherein the air circulation port is arranged on the upper side wall of the storage chamber 1.
[0028] Preferably, in order to facilitate the control of air intake in the storage chamber 1, a first air channel 8 is provided at the upper end of the purified air storage chamber 7 near the air circulation port in this example, and the air circulation port is connected with the purified air storage chamber 7 through the first air channel 8, and the air switch 9 is arranged at the end of the first air channel 8 near the air circulation port.
[0029] The air purification structure 4 of this example is a tank bottom space arranged at the bottom of the feed tank 100 and below the storage chamber 1. The air inlet 2 is connected to the tank bottom space through the second air channel 3. The tank bottom space is used to fill the sterilization and disinfection liquid to sterilize the air entering the purified air storage chamber 7 through the second air channel 3, thereby avoiding the contamination of the materials or culture medium in the storage chamber 1 by bacteria and viruses in the air. The air purification structure 4 is arranged at the bottom of the feed tank 100, which is convenient for the filling and storage of the sterilization and disinfection liquid. When the feed tank 100 of the utility model is moved, it is not easy to cause the overflow of the sterilization and disinfection liquid. Of course, the air purification structure 4 of this example can be arranged at other places in the interlayer between the feed tank 100 and the storage chamber 1, as long as the air inlet 2 and the air circulation port are higher than the air purification structure 4, the sterilization and disinfection liquid will not be backflowed.
[0030] The liquid-stopping structure 6 in this example can be a liquid-stopping switch, or a biological protective membrane that allows air to pass but isolates liquid, etc.
[0031] In this example, the volume of the purified air storage chamber 7 is preferably 3-10 times the volume of the second air inlet channel 3 , so that the air entering the second air inlet channel can be more easily stored in the purified air storage chamber 7 .
[0032] The material of the feed tank 100 and the storage chamber 1 in this example can be metal, ceramic, glass, etc., and the size of the feed tank 100 and the air purification structure 4 can be adjusted according to production needs. When the feed tank 100 is large, or the material in the storage chamber 1 is heavy, in order to improve the strength of the feed tank 100, in this example, a plurality of load-bearing brackets 5 for supporting the storage chamber 1 can be installed at the bottom of the feed tank 100.
[0033] In this example, the large-scale feeding tank can also be provided with a stirring paddle in the storage chamber 1, and a driving device such as a motor for driving the stirring paddle is provided on the feeding tank 100. A heating device for heating the material in the storage chamber 1 can also be provided on the feeding tank 100 to meet various culture or fermentation requirements.
[0034] The working principle of the utility model is:
[0035] The air purification structure 4 is located at the bottom of the tank body of the feeding tank 100, and the air inlet 2 extends from the second air channel 3 from the outer wall of the storage chamber 1 to the bottom of the storage chamber 1, and is connected to the tank bottom space of the air purification structure. The purified air storage chamber 7 is located on the outer wall of the storage chamber 1 opposite to the air inlet 2 pipe, and is connected to the tank bottom space through the stop liquid switch. The purified air storage chamber 7 is integrated with the first air channel 8 for purifying air, and the end of the first air channel 8 is connected to the top of the storage chamber 1 inside the feeding tank through the breathing switch 9.
[0036] For small devices, before sterilizing the feed medium, close the breathing switch 9 and open the liquid stop switch, and sterilize the entire feed tank 100 at high temperature and high pressure. After the feed medium is sterilized, immediately close the liquid stop switch, and then add sterilization and disinfection liquid (such as 84 sterilization liquid, etc.) into the bottom space of the tank until the liquid level rises to the second air channel 3, and stop filling.
[0037] Then, after connecting the feeding pipe (additional setting, not marked in the figure), the breathing switch 9 is opened before feeding. After feeding, the breathing switch 9 and the liquid stop switch are opened normally to carry out the normal biological culture or fermentation process.
[0038] The utility model has a simple structure and reasonable design. The air entering the device (feeding bottle or feeding tank) is processed by the filtering protection system, which is safe and reliable, and can effectively prevent phage and other microbial contamination in biological fermentation experiments and fermentation production processes. The device is simple to operate, saves time and effort, is safe and efficient, and ensures quality and quantity. It can solve the problems that currently seriously affect production efficiency in laboratories and fermentation production processes. The utility model can meet the needs of large-scale microbial fermentation manufacturers, as well as the requirements of small-scale manufacturers, and can also be widely used in fermentation engineering experiments.
[0039] The specific implementation methods described above are preferred implementation methods of the present utility model, and are not intended to limit the specific implementation scope of the present utility model. The scope of the present utility model includes but is not limited to the specific implementation methods. All equivalent changes made in accordance with the present utility model are within the protection scope of the present utility model.
Claims
1. A biosafety protection feeding device, characterized in that: The invention comprises a feeding tank, an air inlet and a discharge port arranged on the feeding tank, a storage chamber connected to the discharge port and used for storing materials is arranged in the feeding tank, an air purification structure and a purified air storage chamber are also arranged in the feeding tank, and a liquid stop structure is arranged between the air purification structure and the purified air storage chamber, wherein: One end of the air purification structure is connected to the air inlet, and the other end is connected to one end of the purified air storage chamber. The other end of the purified air storage chamber is connected to the air flow port above the storage chamber through a breathing switch. External air enters the feeding tank through the air inlet, is purified by the air purification structure, and then enters the purified air storage chamber for storage to provide ventilation in the storage chamber.
2. The biosafety protection feeding device according to claim 1, characterized in that: A top cover is provided on the top of the feeding tank, and the discharge port is arranged on the top cover.
3. The biosafety protection feeding device according to claim 2, characterized in that: The top cover is also provided with a discharge pipe connected with the discharge port, and the discharge pipe or the discharge port of the top cover is provided with a discharge switch.
4. The biosafety protection feeding device according to claim 2, characterized in that: The feed tank comprises a cylindrical first tank body and a conical second tank body arranged above the cylindrical first tank body and integrally arranged with the cylindrical first tank body, and the top cover is arranged on the top surface of the conical second tank body.
5. The biosafety protection feeding device according to claim 1, characterized in that: A stirring paddle is arranged in the material storage chamber, and a driving device for driving the stirring paddle is also arranged on the feeding tank.
6. The biosafety protection feeding device according to claim 1, characterized in that: The feeding tank is also provided with a heating device for heating the material in the storage chamber.
7. The biosafety protection feeding device according to any one of claims 1 to 6, characterized in that: The air inlet is arranged above one side of the outside of the storage chamber, and the purified air storage chamber and the air circulation port are arranged on the other side of the storage chamber, wherein the air circulation port is arranged on the upper side wall of the storage chamber.
8. The biosafety protection feeding device according to claim 7, characterized in that: A first air passage is disposed at the other end of the purified air storage chamber, the air circulation port is communicated with the purified air storage chamber through the first air passage, and the air switch is disposed at the end of the first air passage close to the air circulation port.
9. The biosafety protection feeding device according to claim 7, characterized in that: The air purification structure is a tank bottom space arranged at the bottom of the feeding tank and below the storage chamber. The air inlet is connected to the tank bottom space through a second air channel. The tank bottom space is used to fill with sterilization and disinfection liquid. The volume of the purified air storage chamber is 3-10 times the volume of the second air channel.
10. The biosafety protection feeding device according to claim 9, characterized in that: A plurality of load-bearing brackets for supporting the storage chamber are arranged at the bottom of the feeding tank.