Production system for simultaneously extracting flavone and alkaloid in elaeagnus angustifolia pit

Abstract

The utility model discloses a production system for simultaneously extracting flavones and alkaloids in elaeagnus angustifolia pits. The production system comprises an ultrafine grinder, an enzymolysis extraction tank, a butterfly filter, a cross-flow membrane filter, a rectifying tower and a vacuum low-temperature dryer which are connected in sequence, the number of the enzymolysis extraction tanks is at least two; the ultrafine grinder is communicated with the enzymolysis extraction tank through a diverter valve; an enzyme filling port and an ethanol filling port are formed in the upper part of the enzymolysis extraction tank. According to the method, large-scale production of various functional components of the elaeagnus angustifolia pits is realized, the production energy consumption is reduced, the production cost of the product is reduced, the powdery elaeagnus angustifolia pit extract is prepared, and the biological utilization value of the elaeagnus angustifolia pits is improved.

Description

Technical Field

[0001] The utility model relates to the field of processing of anole seeds, in particular to a production system capable of simultaneously extracting flavonoids and alkaloids from anole seeds. Background Art

[0002] Elaeagnus angustifolia Elaeagnus angustifolia L.), food name. A deciduous tree of the genus Elaeagnus in the family Elaeagnaceae; the stem is high, thornless or thorny, brown-red, shiny, and cylindrical; the leaves are small, thin and papery, oblong-lanceolate, green on the upper side, and grayish white on the lower side; the fruit is oval, pink, and has soft hairs on the surface; the flesh is milky white, powdery, and the fruit stalk is short and sturdy; the fruit tastes sour, slightly sweet, and cool; the bark is sour, slightly bitter, and cool; the stems and roots contain alkaloids; the leaves and branches also contain catechins and epicatechins; the fruit has the effect of strengthening the spleen and stopping diarrhea, and is used for indigestion and other symptoms; the bark has the effect of clearing heat and cooling blood, astringing and relieving pain, and is used for chronic bronchitis, stomachache, enteritis, leucorrhea, etc., and is used externally to stop burns and hemostasis. The core of the Elaeagnus angustifolia has the effect of calming the nerves and helping sleep, and contains a variety of alkaloids and flavonoids.

[0003] Alkaloids are a class of biologically active substances in the core of the Elaeagnus angustifolia, which have a bitter taste. Studies have shown that Elaeagnus angustifolia contains eight alkaloids, including halman, dihydrohalman, 2-methyl-1,2,3,4-tetrahydro-β-carline, elaeagnine, tetrahydrohalol, N-2-methyltetrahydrohalol, halamine and halol.

[0004] Elaeagnus angustifolia contains a variety of flavonoids, including kaempferol, kaempferol derivatives, luteolin, isorhamnetin, tricholoma, coltsfoot ketone, etc. Among them, kaempferol has physiological effects such as hypoglycemic, antibacterial, and anti-inflammatory. Luteolin has anti-inflammatory, anti-allergic, and anti-tumor effects. It is currently believed that the anti-inflammatory activity of luteolin is related to the inhibition of nitric oxide (NO) and other inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), the inhibition of protein tyrosine phosphorylation, and nuclear transcription factor KB (NF-KB)-mediated gene expression. Luteolin can protect oligodendrocytes against hydrogen peroxide-induced oxidative damage in vitro in a dose-dependent manner, strongly inhibit the phagocytosis of myelin by macrophages, significantly reduce the production of cellular reactive oxygen species, reduce the expression of iNOS protein, and significantly reduce the level of NO produced by macrophages at low doses. Luteolin can inhibit vascular endothelial growth factor (VEGF)-induced angiogenesis in rabbit cornea in vivo; it can inhibit tumor growth and angiogenesis in mouse xenograft tumor models; it can inhibit VEGF-induced survival and proliferation of human umbilical vein endothelial cells (HUVECs) in vitro. It can significantly inhibit the proliferation and DNA synthesis of vascular smooth muscle cells induced by platelet-derived growth factor-BB (PDGF-BB), and can block the cell cycle at the G1 phase. It can reduce the tension of phenylephrine-precontracted blood vessels in a concentration-dependent manner, antagonize the vasoconstriction caused by high potassium, and has an arterial vasodilation effect. Its vasodilation effect is non-endothelial-dependent. Its mechanism of action is related to direct inhibition of voltage-dependent calcium channels, receptor-operated calcium channels, intracellular calcium release, and activation of potassium channels, but not to α and β receptors. Isorhamnetin has antioxidant, anti-inflammatory, anti-tumor, antibacterial and antiviral effects. As a potential neuroprotective agent, it can inhibit neurodegeneration of the hippocampal structure in patients with diabetes (DM). Low levels of isorhamnetin can be used as an anti-diabetic drug to promote the uptake of glucose by skeletal muscle cells and maintain blood sugar homeostasis under hyperglycemia. Triticum tectorum has anti-inflammatory, antibacterial, antiviral, antioxidant, hypoglycemic and anti-ulcer effects. Tuberculin has an antioxidant effect. Tuberculin inhibits lipopolysaccharide (LPS)-induced neuronal apoptosis by scavenging reactive oxygen species (ROS). This indicates that Tuberculin is expected to be a lead compound in the study of drugs for neurodegenerative diseases. Coltsfoot ketone also has anti-inflammatory effects. Coltsfoot ketone can inhibit the release of NO, PGE2, and TNF-α by inhibiting the nuclear transcription factor-κB (NF-κB) pathway. The IC50 concentration of coltsfoot ketone to inhibit NO is 8.7 mmol / L, the concentration of PGE2 is reduced to 15.12 ng / mL, and the concentration of TNF-α is reduced to 1.73 ng / mL. It can also inhibit the expression of nitric oxide synthase (iNOS) and COX-2 caused by LPS stimulation of BV-2 microglial cells, thereby inhibiting the release of inflammatory factors.Tussilogen can induce the expression of HO-1 in RAW 264.7 cells, and inhibit the release of NO, TNF-α and PGE2, as well as the expression of iNOS and COX-2 in RAW264.7 cells stimulated by LPS, in a time- and dose-dependent manner. Coltsfoot ketone also has anti-allergic effect. Coltsfoot ketone can significantly inhibit the activity of hyaluronidase. The inhibition rates of low (0.26×10-3 mol / L), medium (0.77×10-3 mol / L), and high (2.30×10-3 mol / L) doses are 29.6%, 45.3%, and 68.6%, respectively. Coltsfoot ketone significantly inhibits the contraction of guinea pig ileum in vitro induced by histamine. The inhibition rates of low (1.54×10-3 mol / L) and high (3.07×10-3) mol / L dose groups are 44.1% and 64.9%, respectively. Coltsfoot ketone significantly inhibits the degranulation of mast cells. The inhibition rates of low (1.92×10-5 mol / L), medium (3.84×10-5 mol / L), and high (7.68×10-5 mol / L) dose groups had inhibition rates of 0, 51.0%, and 100.0%, respectively; coltsfoot ketone inhibited the ear swelling of mice induced by 2,4-dinitrochlorobenzene and the immediate allergic reaction of mouse skin induced by histamine, and the inhibition rates of the low (1.07×10-2 mol / L) and high (2.15×10-2 mol / L) dose groups on ear swelling of mice and immediate allergic reaction of mouse skin were 6.2%, 25.6%, 41.6%, and 52.3%, respectively. Coltsfoot ketone also has anti-cancer effects. Coltsfoot ketone can promote the degradation of β-catenin in HEK293 cells, inhibit the Wnt / β-catenin signaling pathway, and reduce the expression of β-catenin in SW480 and HCT116 colon cancer cells, and reduce the expression of target genes cyclin D 1 and c-myc, thereby inhibiting the proliferation of colon cancer cells. Coltsfoot ketone also has a lipid-lowering effect. Coltsfoot ketone can effectively inhibit the proliferation of rat liver microsomes and HepG2 in vitro The IC50 values ​​of DGAT produced by liver microsomes are 18.8 and 49.1 μmol / L respectively. In the 20 and 30 μmol / L concentrations of cellular triglycerides, 49%, 59% (for radiolabeled glycerol) and 34%, 61% (for radiolabeled acetate) of coltsfoot ketone inhibit its synthesis respectively. Coltsfoot ketone has a lipid-lowering effect and is promising for the treatment of obesity or type 2 diabetes.

[0005] The main methods for preparing flavonoids from Elaeagnus angustifolia include water extraction and alcohol precipitation, where alcohol solution is used to separate flavonoids. This method results in a great loss of flavonoids, a low yield, and a high production cost. Alternatively, it can be extracted with an alcohol organic solvent, concentrated, and passed through a macroporous adsorption resin column, and eluted with a gradient of water and alcohol solutions of different concentrations. However, there is currently no process for simultaneously extracting flavonoids and alkaloids from Elaeagnus angustifolia kernels. Summary of the invention

[0006] The problem to be solved by the utility model is to propose an improved solution or a replacement solution for the shortcomings of the above-mentioned prior art, especially an improved solution or a replacement solution that can simultaneously extract flavonoids and alkaloids from Elaeagnus angustifolia seeds.

[0007] To solve the above problems, the solution adopted by the utility model is as follows: a production system for simultaneously extracting flavonoids and alkaloids from anole seeds, characterized in that the production system comprises an ultrafine pulverizer, an enzymatic extraction tank, a butterfly filter, a cross-flow membrane filter, a distillation tower, and a vacuum low-temperature dryer connected in sequence; the enzymatic extraction tank is provided with at least two; the ultrafine pulverizer is connected to the enzymatic extraction tank through a diverter valve; the upper part of the enzymatic extraction tank is provided with an enzyme filling port and an ethanol filling port. After ultrafine pulverization, the anole seeds are sent into the enzymatic extraction tank through a diverter valve, and after fermentation, ethanol is injected and stirred to dissolve, and then solid impurities are removed by a butterfly filter and a cross-flow membrane filter in sequence, and the filtrate is sent to a distillation tower to recover ethanol, and it is best to send the solid-liquid mixture after passing through the distillation tower to a vacuum low-temperature dryer to remove moisture to obtain a solid mixture of flavonoids and alkaloids.

[0008] Further, the production system for simultaneously extracting flavonoids and alkaloids from the core of the angustifolia is characterized in that the diverter valve is connected to the discharge valve of the superfine pulverizer; the diverter valve includes an outer shell of a barrel-shaped structure, a spiral conveying roller and a motor; the spiral comfort stick is arranged in the outer shell and connected to the motor; a discharge port is arranged at intervals on one side of the ground of the outer shell, each discharge port is connected to a corresponding enzymolysis extraction tank, and an electric control valve is provided at the discharge port. Since enzymolysis takes time, equipping each enzymolysis extraction tank with an ultrafine pulverizer will increase the cost, and manual transportation will reduce production efficiency. Therefore, a diverter valve is designed to allow the angustifolia core powder to be sequentially fed into different enzymolysis extraction tanks, saving time and improving efficiency.

[0009] Furthermore, the production system for simultaneously extracting flavonoids and alkaloids from Elaeagnus angustifolia seeds is characterized in that the enzymatic extraction tank comprises an inner tank and an outer tank, a heating device and a temperature sensor are wrapped around the outer side of the inner tank, and the cavity between the inner tank and the outer tank is filled with insulation material.

[0010] Furthermore, a material turning mechanism is provided in the enzymatic extraction tank; the material turning mechanism is composed of a material shovel, a transmission shaft and a motor; the material shovel is an arc-shaped structure with a gradually increasing bending angle, and the height of the material shovel gradually decreases from the inside to the outside until it is zero.

[0011] The arc-shaped curved structure of the material shovel can turn up the material at the bottom of the enzymatic extraction tank, allowing the enzyme and the material to be fully mixed.

[0012] The technical effects of the utility model are as follows: the superfine pulverizer crushes the angustifolia kernel into particles with a diameter of less than 10um. Then it is distributed to each enzymatic extraction tank through a diverter valve. Multiple enzymatic extraction tanks set in succession share one superfine pulverizer, which rationally utilizes the enzymatic hydrolysis and ethanol extraction time, reduces the setting of the superfine pulverizer, and reduces the cost. In the enzymatic hydrolysis step, 0.3% cellulase (W:W), angustifolia kernel, and 7 times the water (W:V) of the angustifolia kernel are added, and the enzymatic hydrolysis is carried out at a temperature of 45°C for 2 hours. The enzymatic hydrolysis tank is an explosion-proof enzymatic hydrolysis tank, which can be used for the ethanol extraction step. After the enzymatically hydrolyzed angustifolia kernel is cooled to room temperature, 18 times anhydrous ethanol (W:V) is added, 60°C, and the mixing device at the bottom of the extraction tank is turned on to mix the water in the extraction tank and the added anhydrous ethanol to obtain an alcohol extract of about 72%. The extraction is carried out for 2 hours, and then the solid mixture is introduced from the extraction tank into the butterfly filter, and then centrifuged at 1000rpm for 15 minutes. The extract is transported from the butterfly filter to the cross-flow membrane filter for further filtration. Then it is sent to a distillation tower to recover ethanol. Finally, a vacuum low-temperature dryer is used to remove moisture to obtain flavonoids and alkaloids. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is the workflow diagram of the production system for simultaneous extraction of flavonoids and alkaloids from Elaeagnus angustifolia seeds.

[0014] Figure 2 Schematic diagram of the production system structure for simultaneous extraction of flavonoids and alkaloids from Elaeagnus angustifolia seeds.

[0015] Figure 3 Schematic diagram of the diverter valve structure.

[0016] Figure 4 This is a schematic diagram of the enzymatic extraction tank structure.

[0017] Among them, 1 is an ultrafine pulverizer, 2 is an enzymatic extraction tank, 3 is a butterfly filter, 4 is a cross-flow membrane filter, 5 is a distillation tower, 6 is a vacuum low-temperature dryer, 7 is a diverter valve, 21 is an outer tank, 22 is an inner tank, 23 is a heating device, 24 is a transmission shaft, 25 is a shovel, 26 is an enzyme filling port, 27 is an ethanol filling port, 71 is an outer shell, 72 is a spiral conveyor roller, 73 is an electric control valve, and 74 is a motor. DETAILED DESCRIPTION

[0018] The utility model is further described in detail below in conjunction with the accompanying drawings.

[0019] Embodiment: A production system for simultaneously extracting flavonoids and alkaloids from anole seeds, the production system comprising an ultrafine grinder, an enzymatic hydrolysis extraction tank, a butterfly filter, a cross-flow membrane filter, a distillation tower, and a vacuum low-temperature dryer connected in sequence; at least two enzymatic hydrolysis extraction tanks are provided; the ultrafine grinder is connected to the enzymatic hydrolysis extraction tank through a diverter valve; an enzyme filling port and an ethanol filling port are provided on the upper part of the enzymatic hydrolysis extraction tank. After ultrafine grinding, the anole seeds are sent into the enzymatic hydrolysis extraction tank through a diverter valve, and after fermentation, ethanol is injected and stirred to dissolve, and then solid impurities are removed by a butterfly filter and a cross-flow membrane filter in sequence, and the filtrate is sent to a distillation tower to recover ethanol, and preferably the solid-liquid mixture after the distillation tower is sent to a vacuum low-temperature dryer to remove moisture to obtain a solid mixture of flavonoids and alkaloids. The diverter valve is connected to the discharge valve of the ultrafine pulverizer; the diverter valve includes a barrel-shaped outer shell, a spiral conveying roller and a motor; the spiral comfort stick is arranged in the outer shell and connected to the motor; discharge ports are arranged at intervals on one side of the ground of the outer shell, each discharge port is connected to an enzymatic extraction tank, and an electrically controlled valve is provided at the discharge port.

[0020] Production of flavonoids and alkaloids using this production system:

[0021] S1. Wash and dry 20 kg of Elaeagnus angustifolia seeds.

[0022] S2. The washed Elaeagnus angustifolia seeds are ultrafinely ground to a particle diameter of less than 10 μm.

[0023] S3. Use a diverter valve to distribute the superfine powder of Elaeagnus angustifolia kernels into an enzymatic extraction tank. In the enzymatic step, add 60 g of cellulase (W:W), Elaeagnus angustifolia kernels, and 140 kg of water, and perform enzymatic hydrolysis at 45° C. for 2 hours.

[0024] S4. The enzymolysis tank is an explosion-proof enzymolysis tank, which can be used for the ethanol extraction step. After the enzymolyzed Elaeagnus angustifolia kernels are cooled to room temperature, 360 kg of anhydrous ethanol (W:V) is added at 60°C. The mixing device at the bottom of the extraction tank is turned on to mix the water in the extraction tank and the added anhydrous ethanol to obtain an alcohol extract of about 72%, and the extraction is carried out for 2 hours.

[0025] S5. After the solid mixture is introduced from the extraction tank into the butterfly filter component, it is centrifuged at 1000 rpm for 15 minutes.

[0026] S6. Use a vacuum pump to introduce the filtrate into a cross-flow membrane filter for further filtration to remove solid particles.

[0027] S7. Use a vacuum pump to pump the filtrate into a distillation tower to recover ethanol and simultaneously precipitate flavonoids and alkaloids.

[0028] S8. Send the mixture of flavonoids and alkaloids into a vacuum low-temperature dryer to remove moisture.

Claims

1. A production system for simultaneously extracting flavonoids and alkaloids from Elaeagnus angustifolia seeds, characterized in that: The production system comprises an ultrafine pulverizer, an enzymolysis extraction tank, a butterfly filter, a cross-flow membrane filter, a distillation tower and a vacuum low-temperature dryer which are connected in sequence; at least two enzymolysis extraction tanks are provided; the ultrafine pulverizer is connected to the enzymolysis extraction tank via a diverter valve; and an enzyme filling port and an ethanol filling port are provided on the upper part of the enzymolysis extraction tank.

2. The production system for simultaneously extracting flavonoids and alkaloids from Elaeagnus angustifolia seeds according to claim 1, characterized in that: The diverter valve is connected to the discharge valve of the ultrafine pulverizer; the diverter valve includes a barrel-shaped outer shell, a spiral conveying roller and a motor; the spiral comfort stick is arranged in the outer shell and connected to the motor; discharge ports are arranged at intervals on one side of the ground of the outer shell, each discharge port is connected to an enzymatic extraction tank, and an electrically controlled valve is provided at the discharge port.

3. The production system for simultaneously extracting flavonoids and alkaloids from Elaeagnus angustifolia seeds according to claim 1, characterized in that: The enzymatic extraction tank comprises an inner tank and an outer tank. A heating device and a temperature sensor are wound around the outer side of the inner tank. The cavity between the inner tank and the outer tank is filled with heat-insulating materials.

4. The production system for simultaneously extracting flavonoids and alkaloids from Elaeagnus angustifolia seeds according to claim 1, characterized in that: The enzymatic extraction tank is provided with a material turning mechanism; the material turning mechanism is composed of a material shovel, a transmission shaft and a motor; the material shovel is an arc-shaped structure with a gradually increasing bending angle, and the height of the material shovel gradually decreases from the inside to the outside until it is zero.

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