Sample introduction structure of sensor and sensor detection system

Abstract

The utility model relates to the technical field of sensor detection, in particular to a sampling structure of a sensor and a sensor detection system. The sample introduction structure comprises an upper unit, a lower unit and a waterproof breathable film; the waterproof breathable film is arranged between the upper unit and the lower unit; and the liquid inlet and the liquid outlet of the upper unit are respectively connected with the waterproof breathable film. The sensor detection system comprises a sample storage tank, a sampler, an upstream pipeline, a sample introduction structure, a sensor and a downstream pipeline; one end of the sampler is connected with the sample storage tank, and the other end of the sampler is sequentially connected with the upstream pipeline, the sample introduction structure, the sensor and the downstream pipeline. According to the utility model, segmentation bubbles of a sample and a buffer solution in the SPR sample injection process can be removed, and bubbles in a pipeline can be removed without an additional control valve structure or a complex control system. The overall structure is simple, small in size and low in cost.

Description

Technical Field

[0001] The utility model relates to the technical field of sensor detection, in particular to a sensor sample introduction structure and a sensor detection system. Background Art

[0002] When performing detection, the SPR (surface plasmon resonance) type biosensor needs to be driven by a fluid control system to flow the sample to be detected through the surface of the SPR sensor at a certain flow rate to achieve the detection purpose. A complete SPR sample detection also requires the flow of buffer before and after the SPR sample as a reference baseline. That is, during the SPR detection process, the sensor and the pipeline directly connected to the sensor need to pass through the following sequence: buffer - test sample - buffer.

[0003] It is inevitable that the buffer and the sample will mix during the circulation of the test sample and the buffer. However, the affinity detection and data analysis of the SPR sensor are based on the assumption that the buffer and the sample will not mix. Therefore, the design of the SPR instrument must avoid the mixing of the sample and the buffer in the instrument pipeline as much as possible.

[0004] In the pipeline that is not directly connected to the SPR sensor, the buffer and the test sample can usually be separated by bubbles to prevent mixing, and the bubbles in the pipeline are removed by fluid valve control before the sample enters the pipeline directly connected to the SPR sensor. However, in the pipeline directly connected to the sensor, since the SPR sensor cannot allow bubbles to pass through, mixing of the test sample and the buffer is inevitable.

[0005] The commonly used method at present is to shorten the length of the pipeline directly connected to the sensor chip, for example, directly use a microfluidic chip to connect the sensor chip, and complete the removal of bubbles through the microfluidic chip. First, set up a bubble detection device. When bubbles are detected, the electromagnetically controlled three-way valve is switched to the pipeline connected to the waste liquid, and a part of the buffer solution is discharged together with the bubbles. When the liquid is detected again, the bubbles in the three-way valve are completely discharged after a delay, and then the three-way valve is switched back to the sensor pipeline. At the end of the sample, the sensor detects bubbles again, and the three-way valve is switched to the waste liquid, and a small amount of sample and bubbles at the other end of the sample are discharged. However, this method requires the microfluidic chip to be directly connected to the sensor chip, which is not only expensive, but also creates additional requirements for the layout of instruments and equipment. In addition, such a structure is prone to sample volume loss, and a complex control system is also required. Utility Model Content

[0006] In order to overcome the problems existing in the prior art, the utility model aims to provide a sensor sampling structure and a sensor detection system, which have a simple structure and a small size; and can remove bubbles in the pipeline without an additional control system.

[0007] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a sample injection structure of a sensor, comprising an upper unit, a lower unit and a waterproof breathable membrane; the waterproof breathable membrane is arranged between the upper unit and the lower unit; the upper unit comprises a liquid inlet and a liquid outlet; the liquid inlet and the liquid outlet are respectively connected to the waterproof breathable membrane.

[0008] The utility model is further configured as follows: the waterproof and breathable membrane is made of expanded polytetrafluoroethylene.

[0009] The waterproof breathable membrane of the present invention needs to meet the function of being breathable and waterproof, for example, expanded polytetrafluoroethylene (ePTFE) is a mature and widely commercialized waterproof breathable material.

[0010] The utility model is further configured as follows: a placement groove is provided at the bottom of the upper unit, and a liquid channel is provided on the placement groove; the liquid inlet is communicated with one end of the liquid channel; and the liquid outlet is communicated with the other end of the liquid channel.

[0011] The size of the placement slot matches the waterproof breathable membrane. In actual application, the waterproof breathable membrane is placed in the placement slot and then the upper unit and the lower unit are fixed, and the waterproof breathable membrane in the middle is clamped, so that the sealing of the sample injection structure can be better. The liquid channel on the placement slot can accommodate liquid entering from the liquid inlet and passing through the waterproof breathable membrane in the placement slot and then flowing out from the liquid outlet.

[0012] The utility model is further configured as follows: the lower unit is provided with a vacuum chamber and an air pipe connecting port; the vacuum chamber is communicated with the air pipe connecting port.

[0013] The utility model is further configured that: the position of the vacuum chamber corresponds to the position of the liquid channel.

[0014] The utility model is further configured as follows: the sample introduction structure also includes a vacuum pump, and the trachea connecting port is connected to the vacuum pump via a trachea connecting piece.

[0015] The utility model is further configured as follows: the liquid channel is a C-shaped liquid channel, and the liquid channel stroke is increased to ensure that the gas in the liquid channel can be completely separated.

[0016] The utility model is further configured as follows: threaded connection holes are provided at the center and edge of the upper unit; threaded connection holes matching with the upper unit are provided at corresponding positions at the center and edge of the lower unit; the upper unit and the lower unit are connected by bolts.

[0017] The utility model also relates to a sensor detection system, comprising a sample storage tank, a sampler, an upstream pipeline, a sample introduction structure, a sensor and a downstream pipeline; the characteristic is that the sample introduction structure adopts the sample introduction structure of the above-mentioned sensor; the sensor is a surface plasmon resonance sensor;

[0018] One end of the sampler is connected to the sample storage tank, and the other end of the sampler is sequentially connected to the upstream pipeline, the sample introduction structure, the surface plasmon resonance sensor and the downstream pipeline.

[0019] The utility model is further configured as follows: the liquid inlet of the sample injection structure is connected to the upstream pipeline, and the liquid outlet of the sample injection structure is connected to the surface plasmon resonance sensor.

[0020] The beneficial effects of the above technical solution of the utility model are as follows:

[0021] 1. The sample injection structure of the utility model can remove the bubbles separating the sample and the buffer solution during the SPR sample injection process, and the sample injection structure does not require an additional control valve structure or a complex control system to remove the bubbles in the pipeline. The structure is simple, the volume is small, and the cost is low. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for describing the embodiments are briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0023] Figure 1 It is a schematic diagram of the overall structure of the sample injection structure of the utility model.

[0024] Figure 2 It is the front view of the sample injection structure of the utility model.

[0025] Figure 3 for Figure 2 Sectional view at AA.

[0026] Figure 4 It is a wireframe diagram of the upper unit of the utility model.

[0027] Figure 5 for Figure 4 Cross-sectional view at BB in the middle.

[0028] Figure 6 This is a schematic diagram of the sensor detection system described in Example 2 of the present utility model.

[0029] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0030] 1. Upper unit, 11. Liquid channel, 12. Placement slot, 2. Lower unit, 21. Air pipe connector, 22. Vacuum chamber, 23. Air pipe connecting hole, 3. Waterproof breathable membrane, 4. Liquid inlet, 5. Liquid outlet, 6. Threaded connecting hole, 100. Sample storage tank, 200. Sampler, 300. Upstream pipeline, 400. Sample injection structure, 500. Surface plasmon resonance sensor, 600. Downstream pipeline. DETAILED DESCRIPTION

[0031] In order to enable those skilled in the art to better understand the technical solution of the present invention, the following is a clear and complete description of the technical solution of the present invention in conjunction with the drawings of the present invention. Based on the embodiments of the present invention, other similar embodiments obtained by ordinary technicians in the field without creative work should all fall within the scope of protection of the present invention. In addition, the directional words mentioned in the following embodiments, such as "up", "down", "left", "right", etc., are only referenced to the directions of the drawings. Therefore, the directional words used are used to illustrate rather than limit the creation of the present invention.

[0032] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0033] The utility model is further described below in conjunction with the accompanying drawings and preferred embodiments.

[0034] Embodiment 1:

[0035] like Figure 1-Figure 5 As shown, it is a preferred embodiment of the utility model, a sample injection structure of a sensor, comprising an upper unit 1, a lower unit 2 and a waterproof breathable membrane 3; the waterproof breathable membrane 3 is arranged between the upper unit 1 and the lower unit 2; the upper unit 1 comprises a liquid inlet 4 and a liquid outlet 5; the liquid inlet 4 and the liquid outlet 5 are respectively connected to the waterproof breathable membrane 3. The waterproof breathable membrane 3 is made of expanded polytetrafluoroethylene.

[0036] like Figure 1 , Figure 2 , Figure 4As shown, the sample injection structure can be divided into three parts. The upper unit 1 and the lower unit 2 are cylindrical in shape. The center and edge of the upper unit 1 are provided with threaded connection holes 6; the corresponding positions of the center and edge of the lower unit 2 are provided with threaded connection holes 6 that cooperate with the upper unit 1; the upper unit 1 and the lower unit 2 are connected by bolts, and the middle waterproof and breathable membrane 3 is clamped by the upper unit 1 and the lower unit 2.

[0037] Combination Figure 4-Figure 5 As shown, a placement groove 12 is provided at the bottom of the upper unit 1, and a liquid channel 11 is provided on the placement groove 12; the liquid inlet 4 is connected to one end of the liquid channel 11; the liquid outlet 5 is connected to the other end of the liquid channel 11. The liquid channel 11 is a C-shaped liquid channel.

[0038] The size of the placement groove 12 matches the waterproof breathable membrane 3. In actual application, the waterproof breathable membrane 3 is placed in the placement groove 12 and then the upper unit 1 and the lower unit 2 are fixed, and the waterproof breathable membrane 3 in the middle is clamped, so that the sealing of the sample injection structure can be better. The liquid channel 11 on the placement groove 12 can accommodate liquid entering from the liquid inlet 4 and passing through the waterproof breathable membrane 3 in the placement groove 12 and then flowing out from the liquid outlet 5.

[0039] Combination Figure 2 , Figure 3 As shown, the lower unit 2 is provided with a vacuum chamber 22 and an air pipe connection port 23; the vacuum chamber 22 is communicated with the air pipe connection port 23. The position of the vacuum chamber 22 corresponds to the position of the liquid channel 11. The sampling structure also includes a vacuum pump (not shown in the figure), and the air pipe connection port 23 is connected to the vacuum pump through the air pipe connector 21.

[0040] In actual application, the sample is guided by the pipeline from the liquid inlet 4 of the sample injection structure to the waterproof breathable membrane 3. Below the waterproof breathable membrane 3 is the vacuum chamber of the lower unit 2. The vacuum pump is connected to the air pipe connection port 23 of the lower unit 2 to evacuate and form a vacuum. When gas appears in the liquid channel 11, due to the air permeability of the waterproof breathable membrane 3 and the pressure difference between the liquid channel 11 and the vacuum chamber 22 below, the gas will quickly diffuse to the vacuum chamber 22 below and be extracted; when the liquid flows through, because the waterproof breathable membrane 3 has strong hydrophobicity, polar solvents cannot penetrate the micropores therein, and will not leave the liquid channel 11. Finally, the liquid will flow out from the liquid outlet 5, thus realizing the function of removing and segmenting bubbles with zero liquid loss. The whole process has no active mechanical structure, no computer control is required, and zero dead volume.

[0041] Embodiment 2:

[0042] like Figure 6As shown, a sensor detection system includes a sample storage tank 100, a sampler 200, an upstream pipeline 300, a sampling structure 400, a surface plasmon resonance sensor 500 and a downstream pipeline 600; the sampling structure adopts the sampling structure of a sensor described in Example 1;

[0043] One end of the sampler 200 is connected to the sample storage tank 100 , and the other end of the sampler 200 is sequentially connected to the upstream pipeline 300 , the sample injection structure 400 , the surface plasmon resonance sensor 500 and the downstream pipeline 600 .

[0044] The sampler 200 and the surface plasmon resonance sensor 500 are electrically connected to an external fluid control system.

[0045] The liquid inlet 4 of the sample injection structure 400 is connected to the upstream pipeline 300 , and the liquid outlet 5 of the sample injection structure 400 is connected to the surface plasmon resonance sensor 500 .

[0046] In actual application, the sampler 200 takes samples from the sample tank 100. In the sampler 200, the order of sample transportation in the fluid pipeline is air-sample-air. The sampler 200 takes samples from the sample tank 100, and can select whether the sample to be sucked is air or sample by controlling whether the end of its pipeline is located in the liquid in the sample tank 100; for example, the end of the pipeline is controlled to immerse in the liquid of the sample tank 100 or leave the liquid by moving the mechanical arm up and down. After entering the upstream pipeline 300, the fluid control system will remove excess air and inject buffer, retaining a certain amount of bubbles to separate the buffer and sample in the upstream pipeline 300, to prevent the sample from mixing with the buffer during transportation. At this time, the order of samples in the pipeline is buffer-separation bubble-sample-separation bubble-buffer.

[0047] The sample enters the sample injection structure 400 in the above order. Since the waterproof and breathable membrane 3 in the sample injection structure 400 can only pass through gas but not liquid, the volume of the divided bubbles will gradually decrease and eventually disappear when they circulate in the sample injection structure 400. The sample injection structure 400 is also provided with a vacuum pump to create negative pressure, so that the gas can be removed from the pipeline efficiently and quickly. In order to ensure that the gas can be discharged stably, the liquid channel of the sample injection structure 400 will be provided with a certain redundancy in length. When the sample reaches the surface plasmon resonance sensor 500, it is detected in the order of buffer solution-sample-buffer solution in the flow direction, and finally the liquid enters the downstream pipeline 600 to be recovered for other purposes or treated as waste liquid.

[0048] Finally, it should be noted that the above-mentioned embodiments only express one or several implementation methods of the utility model, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the utility model patent. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the utility model, which all belong to the protection scope of the utility model. Therefore, the protection scope of the utility model patent shall be based on the attached claims.

Claims

1. A sample introduction structure of a sensor, characterized in that: It comprises an upper unit, a lower unit and a waterproof breathable membrane; the waterproof breathable membrane is arranged between the upper unit and the lower unit; the upper unit comprises a liquid inlet and a liquid outlet; the liquid inlet and the liquid outlet are respectively connected to the waterproof breathable membrane.

2. A sample introduction structure of a sensor according to claim 1, characterized in that: The waterproof and breathable membrane is made of expanded polytetrafluoroethylene.

3. The sample introduction structure of a sensor according to claim 1, characterized in that: A placement groove is provided at the bottom of the upper unit, and a liquid channel is provided on the placement groove; the liquid inlet is communicated with one end of the liquid channel; and the liquid outlet is communicated with the other end of the liquid channel.

4. A sample introduction structure of a sensor according to claim 3, characterized in that: The lower unit is provided with a vacuum chamber and an air pipe connecting port; the vacuum chamber is communicated with the air pipe connecting port.

5. A sample introduction structure of a sensor according to claim 4, characterized in that: The position of the vacuum chamber corresponds to the position of the liquid channel.

6. A sample injection structure for a sensor according to claim 5, characterized in that: The sample injection structure also includes a vacuum pump, and the trachea connecting port is connected to the vacuum pump via a trachea connecting piece.

7. The sample introduction structure of a sensor according to claim 3, characterized in that: The liquid channel is a C-shaped liquid channel.

8. The sample introduction structure of a sensor according to claim 1, characterized in that: The center and edge of the upper unit are provided with threaded connection holes; the corresponding positions of the center and edge of the lower unit are provided with threaded connection holes matching with the upper unit; the upper unit and the lower unit are connected by bolts.

9. A sensor detection system, comprising a sample storage tank, a sampler, an upstream pipeline, a sample introduction structure, a sensor and a downstream pipeline; characterized in that: The sampling structure adopts the sampling structure of a sensor according to any one of claims 1 to 8, and the sensor is a surface plasmon resonance sensor; One end of the sampler is connected to the sample storage tank, and the other end of the sampler is sequentially connected to the upstream pipeline, the sample introduction structure, the surface plasmon resonance sensor and the downstream pipeline.

10. A sensor detection system according to claim 9, characterized in that: The liquid inlet of the sample injection structure is connected to the upstream pipeline, and the liquid outlet of the sample injection structure is connected to the surface plasmon resonance sensor.

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