Reaction tank liquid level judgment system of FISH pretreatment equipment
By designing a reaction tank liquid level judgment system in the FISH pretreatment equipment, using the current change of the drain pump and the multi-sensor structure, the problem of inaccurate liquid level judgment in the prior art is solved, and high-accurate liquid level monitoring is achieved.
Patent Information
- Application Number
- CN202420945184.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-30
- Publication Date
- 2025-03-14
- Estimated Expiration
- 2034-04-30
AI Technical Summary
The existing methods for judging the liquid level of the reaction tank have problems such as inaccurate, wasted reagents and easy equipment damage, and it is difficult to effectively judge the height of the reagent liquid level in the reaction tank.
A liquid level judgment system for the reaction tank of FISH pretreatment equipment is designed. The liquid level height is determined by setting the first liquid discharge port and the second liquid inlet port, and the operating current changes of the liquid discharge pump. Combining multiple sensors and branch tube structures, the accuracy of the judgment is improved.
Accurate judgment of the height of the reagent liquid in the reaction tank is achieved, the accuracy of judgment is improved, and it is not affected by the physical properties of the reagent, avoiding equipment damage and reagent waste.
Smart Images
Figure CN222618334U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of in vitro diagnostic technology, in particular to a liquid level judgment system for a reaction tank of FISH pre-treatment equipment. Background Art
[0002] Conventional paraffin-embedded tissue sections (conventional sections) are the most widely used method in conventional histological section preparation techniques. In pathology, they are often used to study, observe and judge the morphological changes of cell tissues. In combination with immunological techniques, they can be used for qualitative and localized observation and research of macromolecular substances such as polypeptides and proteins in cell tissues in tissue sections. In combination with FISH methods, they can be used for gene localization and status analysis in cells. They are currently widely used in research in multidisciplinary fields.
[0003] In conventional FISH (fluorescence in situ hybridization) testing, paraffin-embedded tissue sections need to be processed through steps such as dewaxing, penetrant penetration, pepsin digestion, washing, and draining, and need to be processed in a reaction tank.
[0004] In the experiment, it is necessary to ensure that the reagents entering the reaction tank effectively soak the samples, and the liquid level cannot be too high or even overflow outside the reaction tank. Generally, the following schemes are used to judge the liquid level in the reaction tank: 1. Use a peristaltic pump to fill the liquid, and judge the liquid filling height in the tank by the liquid filling time. The number of samples placed in the reaction tank will affect the liquid level. The more samples placed, the more space is occupied in the tank. The higher the liquid level is for the same liquid filling time. As the peristaltic pump is used for a longer time, the more serious the rolling of the pipeline will also lead to inaccurate liquid filling. 2. Float switch. The float switch can accurately judge the liquid level of some reagents, but for reagents with low density and low buoyancy such as dewaxing agents, there may be slow reactions and misjudgments. The float switch occupies extra space and wastes reagents. 3. Photoelectric liquid level switch. Photoelectric switches generally react accurately and quickly, but the situation in the reaction tank is more complicated. Photoelectric switches are prone to accumulate water mist and water droplets in the reaction tank after repeated high and low temperatures, causing misjudgments. 4. Pressure switch. The pressure switch is installed at the bottom of the reaction tank. The higher the liquid level, the greater the feedback pressure. However, the alternation of high and low temperatures in the reaction tank will cause the temperature drift of the pressure switch to cause inaccurate measurement. Complex algorithms are needed to solve this problem. The pressure switch will be damaged if it works at high temperature in the reaction tank for a long time. At present, there are problems to varying degrees in the methods for judging the liquid level of the reaction tank. Utility Model Content
[0005] Based on the above description, the utility model provides a system for judging the liquid level of a reaction tank of a FISH pre-treatment device to solve the problems existing in the method for judging the liquid level height of a reaction tank in the related art.
[0006] The technical solution of the utility model to solve the above technical problems is as follows:
[0007] The present application provides a FISH pre-treatment equipment reaction tank liquid level judgment system, the technical solution adopted is as follows:
[0008] A liquid level judgment system for a reaction tank of a FISH pre-treatment device, comprising:
[0009] A reaction tank, comprising a first liquid inlet, a first liquid discharge port and a second liquid inlet, wherein the first liquid inlet is located below a set liquid level, the first liquid discharge port is at the same height as the set liquid level, and the second liquid inlet is not lower than the set liquid level;
[0010] a liquid inlet tube, one end of which is connected to the first liquid inlet and the other end of which is used to connect to the reagent container;
[0011] a first liquid discharge pipe, one end of which is connected to the first liquid discharge port, and the other end of which is connected to the input end of the liquid discharge pump;
[0012] A second liquid discharge pipe, one end of which is connected to the second liquid inlet, and the other end of which is connected to the output end of the liquid discharge pump;
[0013] Wherein, when adding reagents into the reaction tank, it is suitable to control the liquid inlet pump to be closed according to the change of the operating current of the liquid discharge pump.
[0014] Preferably, at least two first liquid drain ports are provided, the at least two first liquid drain ports are spaced apart in a plane, and the at least two first liquid drain ports are connected to the first liquid drain pipe via branch pipes, respectively.
[0015] Preferably, a sensor is provided in the branch pipe, and the sensor is used to detect whether there is liquid flowing in the branch pipe.
[0016] Preferably, a plurality of the first liquid discharge ports are provided, and the plurality of the first liquid discharge ports are distributed at intervals along the circumference of the reaction tank.
[0017] Preferably, the reaction tank also includes a second drain port, which is arranged at the bottom of the reaction tank, the input end of the drain pump is connected to the first drain pipe through a first valve, the output end of the drain pump is connected to the second drain pipe through a second valve, the second drain port is connected to a third drain pipe, the third drain pipe is connected to the first valve, the second valve is connected to a fourth drain pipe, the fourth drain pipe is used to be connected to a waste liquid container, and is suitable for controlling the input end of the drain pump to be connected to the first drain pipe or the third drain pipe through the first valve, and controlling the output end of the drain pump to be connected to the second drain pipe or the fourth drain pipe through the second valve.
[0018] Preferably, the first liquid discharge port and the second liquid inlet are spaced apart in a plane.
[0019] Preferably, the second liquid inlet is opened on the side wall of the reaction tank, and a conduit is provided on the inner wall of the reaction tank at the second liquid inlet, the conduit is arranged around the second liquid inlet, one end of the conduit is connected with the second liquid inlet, and the other end extends upward at an angle, and a leakage hole is provided at the bottom of the end of the conduit connected with the second liquid inlet.
[0020] Compared with the prior art, the technical solution of this application has the following beneficial technical effects:
[0021] 1. The present application sets a first liquid discharge port and a second liquid inlet. During the process of injecting reagents into the reaction tank through the first liquid inlet, the liquid discharge pump is kept in the started state. When the reagents in the reaction tank do not reach the set liquid level, the first liquid discharge pipe inhales air from the first liquid discharge port and discharges the air from the second liquid inlet to the reaction tank through the second liquid discharge pipe. When the reagents in the reaction tank reach the set liquid level, the first liquid discharge pipe inhales the reagents from the first liquid discharge port, and after passing through the liquid discharge pump, it is returned to the reaction tank from the second liquid inlet through the second liquid discharge pipe. During the process of the liquid discharge pump changing from conveying air to conveying reagents, the working current of the liquid discharge pump will undergo a large jump. By detecting the change in the operating current of the liquid discharge pump, it can be determined whether the reagent liquid level in the reaction tank has reached the set height, with high accuracy and without being affected by the physical properties of the reagents.
[0022] 2. The present application is provided with a plurality of first liquid discharge ports. Since the reagent liquid level will fluctuate when the reagent is injected into the reaction tank, in order to accurately judge the liquid level height, the plurality of first liquid discharge ports are spaced apart in the plane, and each first liquid discharge port is connected to the first liquid discharge pipe through a branch pipe, and a sensor is provided in the branch pipe to detect whether there is liquid flowing in the branch pipe. The control system determines that the liquid level in the reaction tank has reached the set height based on the signals of the plurality of sensors when there is liquid flowing in the plurality of branch pipes and the operating current change of the liquid discharge pump reaches the set threshold, thereby improving the accuracy of the liquid level judgment. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 A schematic diagram of the structure of a liquid level determination system for a reaction tank of a FISH pre-treatment device provided by an embodiment of the utility model;
[0024] Figure 2 A schematic structural diagram of a second liquid inlet and a conduit in a liquid level judgment system in a reaction tank of a FISH pre-treatment device provided in an embodiment of the utility model.
[0025] In the accompanying drawings, the components represented by the reference numerals are listed as follows:
[0026] 1. Reaction tank; 11. First liquid inlet; 12. First liquid discharge port; 13. Second liquid inlet; 14. Second liquid discharge port; 15. Conduit; 2. Liquid inlet pipe; 3. First liquid discharge pipe; 4. Second liquid discharge pipe; 5. Liquid discharge pump; 6. Third liquid discharge pipe; 7. First valve; 8. Second valve; 9. Fourth liquid discharge pipe. DETAILED DESCRIPTION
[0027] In order to facilitate understanding of the present application, the present application will be described more fully below with reference to the relevant drawings. Embodiments of the present application are provided in the drawings. However, the present application can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thorough and comprehensive.
[0028] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application.
[0029] It will be appreciated that spatial relationship terms such as "under," "beneath," "below," "under," "above," "above," etc., may be used herein to describe the relationship of an element or feature shown in the figures to other elements or features. It will be appreciated that, in addition to the orientations shown in the figures, spatial relationship terms also include different orientations of the device in use and operation. For example, if the device in the accompanying drawings is flipped, an element or feature described as "under other elements" or "under it" or "under it" will be oriented as being "above" the other elements or features. Thus, the exemplary terms "under" and "under" may include both upper and lower orientations. In addition, the device may also include additional orientations (e.g., rotated 90 degrees or other orientations), and the spatial descriptors used herein are interpreted accordingly.
[0030] It should be noted that when an element is considered to be "connected" to another element, it can be directly connected to the other element, or connected to the other element through an intermediate element. The "connection" in the following embodiments should be understood as "electrical connection", "communication connection", etc. if the connected circuits, modules, units, etc. have electrical signals or data transmission between each other.
[0031] When used herein, the singular forms "a", "an", and "said / the" may also include plural forms, unless the context clearly indicates otherwise. It should also be understood that the terms "include / comprise" or "have" etc. specify the presence of stated features, wholes, steps, operations, components, parts or combinations thereof, but do not exclude the possibility of the presence or addition of one or more other features, wholes, steps, operations, components, parts or combinations thereof.
[0032] Reference Figure 1-2 As shown, the embodiment of the present application provides a FISH pretreatment equipment reaction tank liquid level judgment system, which includes a reaction tank 1, a liquid inlet pipe 2, a first liquid discharge pipe 3 and a second liquid discharge pipe 4, the reaction tank 1, which includes a first liquid inlet port 11, a first liquid discharge port 12 and a second liquid inlet port 13, the first liquid inlet port 11 is located below the set liquid level, the first liquid discharge port 12 is at the same height as the set liquid level, the second liquid inlet port 13 is not lower than the set liquid level, one end of the liquid inlet pipe 2 is connected to the first liquid inlet port 11, and the other end is used to connect to the reagent container, one end of the first liquid discharge pipe 3 is connected to the first liquid discharge port 12, and the other end is connected to the input end of the liquid discharge pump 5, one end of the second liquid discharge pipe 4 is connected to the second liquid inlet port 13, and the other end is connected to the output end of the liquid discharge pump 5.
[0033] Reference Figure 1 As shown, during the process of the liquid inlet pipe 2 injecting reagent into the reaction tank 1 through the first liquid inlet port 11, the drainage pump 5 remains in the started state. When the reagent in the reaction tank 1 has not reached the set liquid level, the first drainage pipe 3 inhales air from the first drainage port 12, and discharges it into the reaction tank 1 from the second liquid inlet port 13 through the second drainage pipe 4. When the reagent in the reaction tank 1 reaches the set liquid level, the first drainage pipe 3 inhales the reagent from the first drainage port 12, and after passing through the drainage pump 5, it is returned to the reaction tank 1 from the second liquid inlet port 13 through the second drainage pipe 4. During the process of the drainage pump 5 changing from conveying air to conveying reagent, the working current of the drainage pump 5 will undergo a large jump. By detecting the change in the operating current of the drainage pump 5, it can be determined that the reagent liquid level in the reaction tank 1 has reached the set height, with high accuracy and is not affected by the physical properties of the reagent.
[0034] Specifically, the liquid inlet pipe 2 is connected to the output end of the liquid inlet pump, and the input end of the liquid inlet pump is connected to multiple reagent containers through pipelines. Reagents can be pumped out from different reagent containers and injected into the reaction tank 1 as needed.
[0035] Reference Figure 1As shown, further, since the liquid level will fluctuate when the reagent is injected into the reaction tank 1, when only one first liquid discharge port 12 is provided, the liquid level may not reach the set height when stationary, but the first liquid discharge port 12 may absorb the reagent due to the fluctuation of the liquid level, resulting in a misjudgment. Therefore, in order to avoid misjudgment, at least two first liquid discharge ports 12 are provided, at least two first liquid discharge ports 12 are spaced apart in the plane, at least two first liquid discharge ports 12 are respectively connected to the first liquid discharge pipe 3 through branch pipes, and a sensor is provided in the branch pipe, and the sensor is used to detect whether there is liquid flowing in the branch pipe.
[0036] Reference Figure 1 As shown, specifically, a plurality of first liquid discharge ports 12 are provided, and the plurality of first liquid discharge ports 12 are spaced apart circumferentially along the reaction tank 1. When the static liquid level of the reagent in the reaction tank 1 has not reached the set height, when some branch pipes inhale the reagent from the first liquid discharge port 12 under the condition of liquid level fluctuation, some sensors are triggered, and at this time, the amount of reagent inhaled is small, and the operating current of the liquid discharge pump 5 changes little. The control system receives the signal and determines that the liquid level has not reached the set height, and continues to inject the reagent into the reaction tank 1. When liquid flows in a plurality of branch pipes, it indicates that the static liquid level of the reagent has reached the set height. At this time, more reagent is inhaled, and the operating current of the liquid discharge pump 5 changes greatly. The operating current change of the liquid discharge pump 5 reaches the set threshold, that is, it is determined that the liquid level in the reaction tank 1 has reached the set height, and the injection of reagent into the reaction tank 1 is stopped, thereby improving the accuracy of liquid level judgment.
[0037] Reference Figure 1-2 As shown, further, in the process of injecting reagent into the reaction tank 1, the drainage pump 5 runs the second drainage pipe 4 to discharge air from the second drainage port 14. In order to avoid the fluctuation of the reagent liquid level caused by the discharged air, the first drainage port 12 and the second liquid inlet 13 are distributed at intervals in the plane. At the same time, the second liquid inlet 13 is opened on the side wall of the reaction tank 1, and a conduit 15 is arranged at the second liquid inlet 13 on the inner wall of the reaction tank 1. The conduit 15 is arranged around the second liquid inlet 13. One end of the conduit 15 is connected to the second liquid inlet 13, and the other end extends upward at an angle. The end of the conduit 15 connected to the second liquid inlet 13 is provided with a leakage hole at the bottom. When the second drain pipe 4 outputs air from the second drain port 14, the output air enters the conduit 15, and the air is blown upward through the conduit 15, thereby reducing the disturbance to the reagent liquid level. When the second drain pipe 4 outputs the reagent from the second drain port 14, the reagent flows back under the action of gravity and leaks out from the leakage hole into the reaction tank 1, thereby reducing the fluctuation of the reagent liquid level to improve the accuracy of liquid level monitoring.
[0038] Reference Figure 1As shown, further, the reaction tank 1 also includes a second drainage port 14, the second drainage port 14 is arranged at the bottom of the reaction tank 1, the input end of the drainage pump 5 is connected to the first drainage pipe 3 through the first valve 7, the output end of the drainage pump 5 is connected to the second drainage pipe 4 through the second valve 8, the second drainage port 14 is connected to the third drainage pipe 6, the third drainage pipe 6 is connected to the first valve 7, the second valve 8 is connected to the fourth drainage pipe 9, the fourth drainage pipe 9 is used to be connected to the waste liquid container, and is suitable for controlling the input end of the drainage pump 5 to be connected to the first drainage pipe 3 or the third drainage pipe 6 through the first valve 7, and controlling the output end of the drainage pump 5 to be connected to the second drainage pipe 4 or the fourth drainage pipe 9 through the second valve 8. Specifically, the first valve 7 and the second valve 8 are both three-way valves. During the process of injecting reagents into the reaction tank 1, the input end of the drainage pump 5 is connected to the first drainage pipe 3, and the output end is connected to the second drainage pipe 4. The reagents pumped out from the reaction tank 1 are returned to the reaction tank 1. When drainage is required after the reaction is completed, the input end of the drainage pump 5 is connected to the third drainage pipe 6, and the output end is connected to the fourth drainage pipe 9. The reagents are pumped out from the bottom of the reaction tank 1 and input into the waste liquid container through the fourth drainage pipe 9 to discharge the reagents in the reaction tank 1.
[0039] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.
Claims
1. A FISH pre-treatment equipment reaction tank liquid level judgment system, characterized in that: include: A reaction tank (1), comprising a first liquid inlet (11), a first liquid discharge port (12) and a second liquid inlet (13), wherein the first liquid inlet (11) is located below a set liquid level, the first liquid discharge port (12) is at the same height as the set liquid level, and the second liquid inlet (13) is not lower than the set liquid level; a liquid inlet pipe (2), one end of which is connected to the first liquid inlet (11), and the other end of which is used to connect to a reagent container; A first liquid discharge pipe (3), one end of which is connected to the first liquid discharge port (12), and the other end of which is connected to the input end of the liquid discharge pump (5); a second liquid discharge pipe (4), one end of which is connected to the second liquid inlet (13), and the other end of which is connected to the output end of the liquid discharge pump (5); When adding reagents into the reaction tank (1), it is suitable to control the liquid inlet pump to be closed according to the change of the operating current of the liquid discharge pump (5).
2. The FISH pre-treatment equipment reaction tank liquid level judgment system according to claim 1, characterized in that: At least two of the first liquid discharge ports (12) are provided, the at least two first liquid discharge ports (12) are spaced apart in a plane, and the at least two first liquid discharge ports (12) are respectively connected to the first liquid discharge pipe (3) via branch pipes.
3. The FISH pre-treatment equipment reaction tank liquid level judgment system according to claim 2, characterized in that: A sensor is arranged in the branch pipe, and the sensor is used to detect whether there is liquid flowing in the branch pipe.
4. The FISH pre-treatment equipment reaction tank liquid level judgment system according to claim 2, characterized in that: A plurality of the first liquid discharge ports (12) are provided, and the plurality of the first liquid discharge ports (12) are distributed at intervals along the circumference of the reaction tank (1).
5. The FISH pre-treatment equipment reaction tank liquid level judgment system according to claim 1, characterized in that: The reaction tank (1) further comprises a second drainage port (14), wherein the second drainage port (14) is arranged at the bottom of the reaction tank (1); the input end of the drainage pump (5) is connected to the first drainage pipe (3) through a first valve (7); the output end of the drainage pump (5) is connected to the second drainage pipe (4) through a second valve (8); the second drainage port (14) is connected to a third drainage pipe (6), wherein the third drainage pipe (6) is connected to the first valve (7); the second valve (8) is connected to a fourth drainage pipe (9); the fourth drainage pipe (9) is used to be connected to a waste liquid container and is suitable for controlling the input end of the drainage pump (5) to be connected to the first drainage pipe (3) or the third drainage pipe (6) through the first valve (7); and controlling the output end of the drainage pump (5) to be connected to the second drainage pipe (4) or the fourth drainage pipe (9) through the second valve (8).
6. The FISH pre-treatment equipment reaction tank liquid level judgment system according to claim 1, characterized in that: The first liquid discharge port (12) and the second liquid inlet (13) are spaced apart in a plane.
7. The FISH pre-treatment equipment reaction tank liquid level judgment system according to claim 1, characterized in that: The second liquid inlet (13) is provided on the side wall of the reaction tank (1); a conduit (15) is provided on the inner wall of the reaction tank (1) at the second liquid inlet (13); the conduit (15) is arranged around the second liquid inlet (13); one end of the conduit (15) is connected to the second liquid inlet (13); the other end of the conduit (15) extends upward at an angle; a leakage hole is provided at the bottom of the end of the conduit (15) connected to the second liquid inlet (13).