Amino acid derivatization tandem mass spectrometry detection kit

Abstract

The utility model relates to an amino acid derivatization tandem mass spectrometry detection kit which comprises a kit body, the front side of the kit body is open, the rear side of the kit body is provided with a limiting groove, a sliding block capable of moving up and down is arranged in the limiting groove, the top of the sliding block can extend out of the kit body and is hinged to an L-shaped cover, one end of the L-shaped cover can be inserted into the limiting groove, and the other end of the L-shaped cover is hinged to the limiting groove. Supports are fixed to the left side and the right side in the box body respectively, a tray is arranged on the two supports, a plurality of containing grooves are formed in the tray, shifting rods are hinged to the bottoms of the containing grooves, and one sides of the shifting rods are connected with the tray through arc-shaped springs. Various reagent tubes are respectively placed in the placing grooves, when the L-shaped cover is in an open state, the shifting rod can tilt the reagent tubes by a certain angle under the action of the arc-shaped spring, so that the reagent tubes are convenient to take, when the L-shaped cover covers the box body, the arc-shaped spring is compressed to store force, and the reagent tubes are pressed in the placing grooves by the L-shaped cover, so that the reagent tubes are convenient to take. Therefore, the reagent tubes are convenient to store or take.

Description

Technical Field

[0001] This invention relates to whole blood detection technology for various amino acids, and in particular to an amino acid derivatization tandem mass spectrometry detection kit. Background Art

[0002] Currently, a high-throughput rapid derivatization tandem mass spectrometry method for detecting 22 amino acids in whole blood can be established to meet the clinical needs for screening and diagnosis of inherited metabolic diseases. The method involves collecting heel blood from newborns and capillary blood from children onto blank blood collection filter paper, allowing it to fully penetrate, and then drying it. Using a punch, circular blood filter paper discs with a diameter of 3 mm are made on the dried blood filter paper discs (4 quality controls and 1 blank). These discs are placed in a 96-well plate, and 100 μL of an extraction solution prepared by dissolving and diluting in methanol is added to each well. The plate is sealed and shaken at room temperature for 15 min to extract the 22 amino acids from the blood discs. The extraction solution is then transferred to another 96-well plate, heated to 40°C, and dried under nitrogen. 60 μL of derivatization reagent is then added, the plate is covered with a Teflon membrane, and the plate is incubated at 65°C for 20 min. Subsequently, the plate is dried under nitrogen at 40°C, and 100 μL of 80% acetonitrile is added to dissolve the amino acids. The plate is then covered with an aluminum membrane and detected by tandem mass spectrometry.

[0003] Currently, in establishing a high-throughput rapid detection method for 22 amino acids in whole blood using derivatization tandem mass spectrometry, kits are typically required to store extracts, derivatization reagents, and other reagents, and 96-well plates need to be stored. Existing kits are often inconvenient for storing or retrieving reagents and 96-well plates. Utility Model Content

[0004] The purpose of this invention is to provide an amino acid derivatization tandem mass spectrometry detection kit to solve the problem that existing kits are often inconvenient for storing or retrieving reagents and 96-well plates.

[0005] To address the aforementioned problems, this invention provides an amino acid derivatization tandem mass spectrometry detection kit, comprising a box body with an open front and a limiting groove on the rear. A slider that can move up and down is located within the limiting groove. The top of the slider extends out of the box body and is hinged to an L-shaped cover, one end of which can be inserted into the limiting groove. Supports are fixed to the left and right sides of the box body, and a tray is mounted on each support. Multiple placement slots are provided on the tray, and a lever is hinged to the bottom of each placement slot. One side of the lever is connected to the tray via an arc spring. Two support plates are slidably connected between the two supports, with the front ends of each support plate extending out of the box body.

[0006] The amino acid derivatization tandem mass spectrometry detection kit provided by this utility model also has the following technical features:

[0007] Furthermore, one side of the placement slot is connected to a clearance slot.

[0008] Furthermore, the lower surface of the slider is an inclined surface, and a push block is fixed on one side of the lower support plate. The push block can extend into the limiting groove, and the lower surface of the slider can contact the push block.

[0009] Furthermore, a vertical plate is fixed to the upper side of the lower support plate, and a connecting plate is fixed to the lower side of the upper support plate. The front side of the vertical plate can contact the connecting plate.

[0010] Furthermore, the tray has a rectangular groove, and a placement frame is provided inside the rectangular groove.

[0011] Furthermore, the placement frame is provided with multiple round holes and square slots.

[0012] This invention has the following advantages: By placing various reagent tubes in the placement slots, when the L-shaped cover is open, the lever can tilt the reagent tubes at a certain angle under the action of the arc spring, making it easier to pick up the reagent tubes. When the L-shaped cover is on the box, the arc spring is compressed and stored, and the reagent tubes are pressed into the placement slots by the L-shaped cover, making it easier to store or pick up the reagent tubes. The two support plates facilitate the placement of two 96-well plates, and the front end of the support plate can extend through the opening of the box 1, making it easier to store or pick up the 96-well plates. This makes it convenient to use in the high-throughput rapid detection of 22 amino acids in whole blood using derivatization tandem mass spectrometry. Attached Figure Description

[0013] Figure 1 This is an isometric view of an embodiment of the present utility model;

[0014] Figure 2 This is an isometric view of the embodiment of the present invention with the L-shaped cover closed;

[0015] Figure 3 This is a right-side sectional view of the fracture in an embodiment of the present invention;

[0016] Figure 4 This is an embodiment of the present utility model. Figure 2 Right sectional view after removing the L-shaped cover;

[0017] Figure 5 This is a structural diagram of the box body in an embodiment of this utility model. Detailed Implementation

[0018] The present invention will be described in detail below with reference to the accompanying drawings and embodiments. It should be noted that, unless otherwise specified, the embodiments and features in the embodiments of the present invention can be combined with each other.

[0019] like Figures 1 to 5In the embodiment of the amino acid derivatization tandem mass spectrometry detection kit of this utility model shown, the kit includes a box body 1. The front of the box body 1 is open, and a limiting groove is formed on the rear of the box body 1. A slider 2 that can move up and down is provided in the limiting groove. The top of the slider 2 can extend out of the box body 1 and is hinged to an L-shaped cover 3. One end of the L-shaped cover 3 can be inserted into the limiting groove. Supports 4 are fixed on the left and right sides of the box body 1, and a tray 5 is provided on the two supports 4. Multiple placement slots 6 are formed on the tray 5. A lever 7 is hinged to the bottom of the placement slot 6. One side of the lever 7 is connected to the tray 5 via an arc spring 8. There are two sliding support plates 9, the front ends of which can extend out of the box body 1 respectively. By placing various reagent tubes in the placement slots 6 respectively, when the L-shaped cover 3 is open, the lever 7 can tilt the reagent tubes at a certain angle under the action of the arc spring 8, so as to facilitate the removal of the reagent tubes. When the L-shaped cover 3 is on the box body 1, the arc spring 8 is compressed and stored, and the reagent tubes are pressed into the placement slots 6 by the L-shaped cover 3, so as to facilitate the storage or removal of the reagent tubes. The setting of the two support plates 9 makes it easy to place two 96-well plates respectively, and the front ends of the support plates 9 can extend through the opening of the box body 1, so as to facilitate the storage or removal of the 96-well plates.

[0020] In one embodiment of this application, preferably, a relief groove 10 is connected to one side of the placement groove 6. The relief groove 10 facilitates the insertion of fingers into the placement groove 6, thereby making it easier to take out the reagent tube.

[0021] In one embodiment of this application, preferably, the lower surface of the slider 2 is an inclined surface, and a push block 11 is fixed on one side of the lower support plate 9. The push block 11 can extend into the limiting groove, and the lower surface of the slider 2 can contact the push block 11. When the L-shaped cover 3 is in the open state, the L-shaped cover 3 is moved downward so that the L-shaped cover 3 is inserted into the limiting groove, thereby driving the slider 2 to move downward so that the inclined surface of the slider 2 contacts the push block 11, thereby driving the push block 11 and the lower support plate 9 to move forward and extend out of the opening of the box body 1.

[0022] In one embodiment of this application, preferably, a vertical plate 12 is fixed to the upper side of the lower support plate 9, and a connecting plate 13 is fixed to the lower side of the upper support plate 9. The front side of the vertical plate 12 can contact the connecting plate 13. During the forward movement of the lower support plate 9, the front side of the vertical plate 12 can contact the connecting plate 13, thereby driving the upper support plate 9 to move forward and extend out of the box body 1. Finally, both support plates 9 extend out of the box body 1, and the two support plates 9 are stepped, which facilitates the removal of the 96-hole plate on the support plate 9.

[0023] In one embodiment of this application, preferably, a rectangular groove is provided on the tray 5, and a placement frame 14 is provided in the rectangular groove.

[0024] In one embodiment of this application, preferably, the placement frame 14 is provided with a plurality of round holes 15 and square grooves 16. The round holes 15 make it easy to take out the reagent tubes to be used from the placement grooves 6 and insert them into the round holes 15, thereby facilitating the placement of the reagent tubes. The square grooves 16 can hold other miscellaneous items.

[0025] In use, this invention involves placing various reagent tubes in the placement slot 6. When the L-shaped cover 3 is opened, the lever 7, under the action of the arc spring 8, tilts the reagent tubes at a certain angle, making it easier to retrieve them. The opened L-shaped cover 3 is then moved downwards, inserting into the limiting slot. This causes the slider 2 to move downwards, bringing its inclined surface into contact with the push block 11. This, in turn, moves the push block 11 and the lower support plate 9 forward, extending them out of the opening of the box body 1. During the forward movement of the lower support plate 9, the front side of the vertical plate 12 contacts the connecting plate 1. When the two supports 9 are in contact, the upper support plate 9 moves forward and extends out of the box body 1. Finally, both support plates 9 extend out of the box body 1 and are stepped, which facilitates the removal of the 96-well plate on the support plate 9. When the L-shaped cover 3 is placed on the box body 1, the arc spring 8 is compressed and stores force, and the reagent tube is pressed into the placement slot 6 by the L-shaped cover 3. The two support plates 9 are reset, which facilitates the storage or removal of the reagent tube. The setting of the two support plates 9 makes it easy to place two 96-well plates separately, and the front end of the support plate 9 can extend through the opening of the box body 1, which facilitates the storage or removal of the 96-well plate.

[0026] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and not to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. An amino acid derivatization tandem mass spectrometry detection kit, comprising a box body (1), characterized in that: The front side of the box body (1) is open, and a limiting groove is provided on the rear side of the box body (1). A slider (2) that can move up and down is provided in the limiting groove. The top of the slider (2) can extend out of the box body (1) and is hingedly connected to an L-shaped cover (3). One end of the L-shaped cover (3) can be inserted into the limiting groove. Supports (4) are fixed on the left and right sides of the box body (1). A tray (5) is provided on the two supports (4). The tray (5) is provided with a plurality of placement grooves (6). A lever (7) is hingedly connected to the bottom of the placement groove (6). One side of the lever (7) is connected to the tray (5) via an arc spring (8). Two support plates (9) are slidably connected between the two supports (4). The front ends of the two support plates (9) can extend out of the box body (1) respectively.

2. The amino acid derivatization tandem mass spectrometry detection kit according to claim 1, characterized in that: One side of the placement groove (6) is connected to a clearance groove (10).

3. The amino acid derivatization tandem mass spectrometry detection kit according to claim 1, characterized in that: The lower surface of the slider (2) is an inclined surface, and a push block (11) is fixed to one side of the lower support plate (9). The push block (11) can extend into the limiting groove, and the lower surface of the slider (2) can contact the push block (11).

4. The amino acid derivatization tandem mass spectrometry detection kit according to claim 3, characterized in that: A vertical plate (12) is fixed to the upper side of the lower support plate (9), and a connecting plate (13) is fixed to the lower side of the upper support plate (9), and the front side of the vertical plate (12) can contact the connecting plate (13).

5. The amino acid derivatization tandem mass spectrometry detection kit according to claim 1, characterized in that: The tray (5) is provided with a rectangular groove, and a placement frame (14) is provided in the rectangular groove.

6. The amino acid derivatization tandem mass spectrometry detection kit according to claim 5, characterized in that: The placement frame (14) is provided with a plurality of circular holes (15) and square grooves (16).

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