Tumor stem cell identification device

Abstract

The utility model discloses a tumor stem cell identification device which comprises a box body, a plurality of bottle grooves, a plurality of storage grooves, a plurality of circular grooves and a plurality of test tube grooves are formed in the box body, a storage groove is formed in the middle of the box body, and reagent bottles are placed in the bottle grooves. The pressing plate is pressed to drive the piston rod and the piston to move, then the connecting pipe is inserted into the reagent bottle, the pressing plate is loosened, the piston is driven to move in the direction away from the adapter under the resilience force of the spring, accordingly, the fluorescent dye reagent in the reagent bottle is sucked into the containing cavity, and then the fluorescent dye reagent in the containing cavity is discharged into the culture dish. In the process, the fluorescent dye reagent cannot enter the reagent extractor, the reagent extractor cannot be polluted, the reagent extractor can be continuously used only by replacing the adapter, and the adapter is simple in structure, convenient to manufacture and low in manufacturing cost. The replacement of the adapter is more cost-effective than the replacement of the rubber head dropper.

Description

Technical Field

[0001] The utility model relates to the technical field of tumor stem cell identification, in particular to a tumor stem cell identification device. Background Art

[0002] When identifying tumor stem cells, it is necessary to first extract a serum sample from the test subject, then centrifuge the serum sample, pour the centrifuged sample into a cell culture dish, and then add a fluorescent dye reagent to the cell culture dish; gently shake the TSC cell culture dish, and then place the cell culture dish at 22-35°C for incubation for 20-30 minutes; after the incubation is completed, the tester takes out the cell culture dish and uses a fluorescent microscope to observe the staining of the tumor stem cells inside the cell culture dish to obtain the test results.

[0003] Publication No. CN 114408357 B discloses a tumor stem cell identification kit, which provides a No. 1 fixing block in a No. 2 fixing groove inside the box body, and provides a No. 2 fixing block at a corresponding position on the outer surface of a reagent bottle, and combines the No. 2 fixing block with the No. 1 fixing block on the reagent bottle embedded in the No. 2 fixing groove, so that the reagent bottle remains stable during the transportation of the kit, thereby avoiding damage, leakage, and other problems of the reagent bottle due to vibration during transportation.

[0004] The above-mentioned prior art uses a rubber-tipped dropper to absorb the fluorescent dye reagent, but the fluorescent dye reagent added to the cell culture dish is limited in dosage, and the rubber-tipped dropper cannot control the dosage of the absorbed fluorescent dye reagent. Moreover, after use, the rubber-tipped dropper is not convenient to clean due to the existence of its rubber head, because the rubber head is easily corroded during cleaning, and drying and other operations are required after cleaning. In these processes, the rubber-tipped dropper cannot be used, and its use efficiency is low. Utility Model Content

[0005] (I) Purpose of the utility model

[0006] In view of this, the purpose of the utility model is to provide a tumor stem cell identification device, the technical problem to be solved is that the rubber-tipped dropper cannot control the dosage of the absorbed fluorescent dye reagent, and after use, the rubber-tipped dropper is not convenient to clean due to the existence of its rubber head, because the rubber head is easily corroded during cleaning, and after cleaning, it is necessary to dry it again, and the rubber-tipped dropper cannot be used during these processes, and its use efficiency is low.

[0007] (II) Technical solution

[0008] In order to achieve the above technical objectives, the utility model provides a tumor stem cell identification device:

[0009] The box body comprises a box body, wherein a plurality of bottle slots, a plurality of storage slots, a plurality of circular slots and a plurality of test tube slots are provided in the box body, and a storage slot is provided in the middle of the box body, wherein reagent bottles are placed in the plurality of bottle slots, adapters are placed in the plurality of storage slots, culture dishes are placed in the plurality of circular slots, test tubes are placed in the plurality of test tube slots, a reagent extractor is placed in the storage slot, and a control component for controlling the amount of reagent added is provided on the reagent extractor.

[0010] Preferably, the adapter comprises a accommodating chamber, one end of the accommodating chamber is fixed and connected with a connecting pipe, and the other end of the accommodating chamber is fixed and connected with a straw.

[0011] Preferably, the reagent extractor comprises a transparent tube, one end of which is fixed with and connected to a catheter, and the catheter can be inserted into the connecting tube, a rubber pad is glued to the outside of the catheter, a piston is slidably connected inside the transparent tube, and a piston rod is fixed to one side of the piston.

[0012] Preferably, a partition is fixed inside the transparent tube, the piston rod passes through the partition and is slidably connected to the partition, and a pressing plate is fixed to one end of the piston rod away from the piston.

[0013] Preferably, a spring is sleeved on the outer side of the piston rod, and one end of the spring abuts against the partition plate, and the other end of the spring abuts against the pressing plate.

[0014] Preferably, the control component comprises a transparent sleeve threadably connected to the transparent tube, and a dosage line is arranged on the outer side of the transparent sleeve.

[0015] Preferably, the diameter of the pressing plate is larger than the diameter of the transparent sleeve.

[0016] It can be seen from the above technical solutions that the present application has the following beneficial effects:

[0017] 1: Press the button to drive the piston rod and the piston to move, then insert the straw into the reagent bottle, release the button, and drive the piston to move away from the adapter under the spring rebound force, so that the fluorescent dye reagent in the reagent bottle is sucked into the containing chamber, and then the fluorescent dye reagent in the containing chamber is discharged into the culture dish. During this process, the fluorescent dye reagent will not enter the reagent extractor, and the reagent extractor will not be contaminated. Only the adapter needs to be replaced, and the reagent extractor can continue to be used. In addition, the adapter has a simple structure, is easy to manufacture, and has a low manufacturing cost. Compared with the rubber-tipped dropper in the prior art, replacing the adapter is more cost-effective than replacing the rubber-tipped dropper.

[0018] 2: Control the distance that the transparent sleeve moves by rotating the transparent sleeve and observing the dose line. In the normal state of the spring, the distance from the press plate to one end of the transparent tube is constant, and this distance is recorded as A. By observing the dose line, the distance from one end of the transparent sleeve to one end of the transparent tube can be known, and this distance is recorded as B. After pressing the press plate so that the press plate and one side of the transparent sleeve are offset, the value is A minus B. This value is the distance moved under the press plate, that is, the dose of the fluorescent dye reagent absorbed. By rotating the transparent sleeve to change the value of B, the dose of the fluorescent dye reagent can be adjusted according to demand. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are only embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0020] Figure 1 A schematic diagram of the structure of a tumor stem cell identification device provided by the utility model;

[0021] Figure 2 A schematic diagram of the disassembled structure of a tumor stem cell identification device provided by the utility model;

[0022] Figure 3 A schematic diagram of the structure of the adapter provided by the utility model;

[0023] Figure 4 A schematic cross-sectional structure diagram of a reagent extractor provided by the utility model;

[0024] Figure 5 This is a structural schematic diagram of the adapter provided by the utility model and the reagent extractor when connected.

[0025] Description of the drawings: 1. Box body; 2. Bottle slot; 3. Storage slot; 4. Storage slot; 5. Round slot; 6. Test tube slot; 7. Reagent bottle; 8. Adapter; 81. Pipette; 82. Receiving chamber; 83. Connecting tube; 9. Reagent extractor; 91. Transparent tube; 92. Catheter; 93. Piston; 94. Piston rod; 10. Culture dish; 11. Test tube; 12. Partition; 13. Press plate; 14. Spring; 15. Transparent sleeve; 16. Dosage line. DETAILED DESCRIPTION

[0026] The following description is merely exemplary in nature and is not intended to limit the present disclosure, application, and use. It should be understood that in all of these figures, the same or similar reference numerals indicate the same or similar parts and features. The various drawings only schematically represent the concepts and principles of the embodiments of the present disclosure, and do not necessarily show the specific dimensions and proportions of the various embodiments of the present disclosure. Specific parts in specific drawings may be exaggerated to illustrate the relevant details or structures of the embodiments of the present disclosure.

[0027] Reference Figure 1-5 :

[0028] The utility model provides a tumor stem cell identification device, comprising a box body 1, wherein the box body 1 is provided with a plurality of bottle slots 2, a plurality of storage slots 3, a plurality of circular slots 5 and a plurality of test tube slots 6, and a storage slot 4 is provided in the middle of the box body 1, wherein reagent bottles 7 are placed in the plurality of bottle slots 2, adapters 8 are placed in the plurality of storage slots 3, culture dishes 10 are placed in the plurality of circular slots 5, test tubes 11 are placed in the plurality of test tube slots 6, a reagent extractor 9 is placed in the storage slot 4, and a control component for controlling the amount of reagent added is provided on the reagent extractor 9.

[0029] When in use, first take out the test tube 11 from the test tube slot 6, then inject the serum sample of the test object into the test tube 11, then centrifuge the serum sample in the test tube 11, then take out the supernatant and pour it into the culture dish 10, then take out the reagent bottle 7 in the bottle slot 2, inject the fluorescent dye reagent in the reagent bottle 7 into the culture dish 10, then slightly shake the culture dish 10, and then incubate it. After the incubation is completed, the tester uses a fluorescent microscope to observe the staining of the tumor stem cells inside the culture dish 10 to obtain the test results.

[0030] Among them, the adapter 8 includes a accommodating chamber 82, one end of the accommodating chamber 82 is fixed and connected with a connecting tube 83, and the other end of the accommodating chamber 82 is fixed and connected with a straw 81, the reagent extractor 9 includes a transparent tube 91, one end of the transparent tube 91 is fixed and connected with a conduit 92, and the conduit 92 can be inserted into the connecting tube 83, a rubber pad is glued to the outside of the conduit 92, a piston 93 is slidably connected in the transparent tube 91, and a piston rod 94 is fixed on one side of the piston 93, a partition 12 is fixed in the transparent tube 91, the piston rod 94 penetrates the partition 12 and is slidably connected to the partition 12, a pressing plate 13 is fixed to the end of the piston rod 94 away from the piston 93, a spring 14 is sleeved on the outside of the piston rod 94, and one end of the spring 14 is against the partition 12, and the other end of the spring 14 is against the pressing plate 13.

[0031] During use, when it is necessary to inject the fluorescent dye reagent in the reagent bottle 7 into the culture dish 10, first take out the reagent extractor 9 and the adapter 8, then insert the catheter 92 into the connecting tube 83, then press the pressing plate 13 to drive the piston rod 94 and the piston 93 to move, and then insert the straw 81 into the reagent bottle 7, and then release the pressing plate 13. At this time, the piston 93 is driven to move away from the adapter 8 under the rebound force of the spring 14, so that the fluorescent dye reagent in the reagent bottle 7 is sucked into the accommodating cavity 82, and then the straw 81 is pulled out of the reagent bottle 7, and then the adapter 8 and the reagent extractor 9 are moved to move the straw 81 to the culture dish 10. At the top, the pressing plate 13 is pressed to drive the piston rod 94 and the piston 93 to move, so that the piston 93 squeezes the air in the transparent tube 91 into the accommodating chamber 82, thereby discharging the fluorescent dye reagent in the accommodating chamber 82 into the culture dish 10. When the fluorescent dye reagent is extracted, the fluorescent dye reagent will not enter the reagent extractor 9, and the reagent extractor 9 will not be contaminated. Therefore, only the adapter 8 needs to be removed to replace the adapter 8, and the reagent extractor 9 can continue to be used. Moreover, the adapter 8 has a simple structure, is easy to manufacture and has a low manufacturing cost. Compared with the rubber-tipped dropper in the prior art, replacing the adapter 8 is more cost-effective than replacing the rubber-tipped dropper.

[0032] In addition, the control component includes a transparent sleeve 15 threadedly connected to the transparent tube 91 , a dosage line 16 is arranged on the outside of the transparent sleeve 15 , and the diameter of the pressing plate 13 is larger than the diameter of the transparent sleeve 15 .

[0033] During use, since there is a limit on the dosage when extracting the fluorescent dye reagent, when extracting the required dosage, the distance moved by the transparent sleeve 15 is controlled by rotating the transparent sleeve 15 and observing the dosage line 16. Since the distance from the pressing plate 13 to one end of the transparent tube 91 is constant in the normal state of the spring 14, this distance is recorded as A. By observing the dosage line 16, the distance from one end of the transparent sleeve 15 to one end of the transparent tube 91 can be known, and this distance is recorded as B. When the pressing plate 13 is pressed so that the pressing plate 13 and one side of the transparent sleeve 15 are against each other, the value is obtained by subtracting B from A. This value is the distance moved by the pressing plate 13, that is, the dosage of the fluorescent dye reagent absorbed. By changing the position of the transparent sleeve 15, that is, changing the value of B, the dosage of the fluorescent dye reagent is adjusted.

[0034] The exemplary implementation scheme of the present disclosure is described in detail above with reference to the preferred embodiments. However, it can be understood by those skilled in the art that, without departing from the concept of the present disclosure, various modifications and variations can be made to the above-mentioned specific embodiments, and various technical features and structures proposed in the present disclosure can be combined in various ways without exceeding the protection scope of the present disclosure, which is determined by the attached claims.

Claims

1. A tumor stem cell identification device, comprising a box body (1), characterized in that: The box body (1) is provided with a plurality of bottle slots (2), a plurality of storage slots (3), a plurality of circular slots (5) and a plurality of test tube slots (6), and a storage slot (4) is provided in the middle of the box body (1), a plurality of bottle slots (2) are each provided with a reagent bottle (7), a plurality of storage slots (3) are each provided with an adapter (8), a plurality of circular slots (5) are each provided with a culture dish (10), a plurality of test tube slots (6) are each provided with a test tube (11), a reagent extractor (9) is provided in the storage slot (4), and a control component for controlling the amount of reagent added is provided on the reagent extractor (9).

2. A tumor stem cell identification device according to claim 1, characterized in that: The adapter (8) comprises a containing chamber (82), one end of which is fixed and connected to a connecting pipe (83), and the other end of which is fixed and connected to a suction pipe (81).

3. A tumor stem cell identification device according to claim 2, characterized in that: The reagent extractor (9) comprises a transparent tube (91), one end of which is fixed and connected to a conduit (92), and the conduit (92) can be inserted into a connecting tube (83), a rubber pad is glued to the outer side of the conduit (92), a piston (93) is slidably connected inside the transparent tube (91), and a piston rod (94) is fixed to one side of the piston (93).

4. A tumor stem cell identification device according to claim 3, characterized in that: A partition (12) is fixed inside the transparent tube (91), the piston rod (94) penetrates the partition (12) and is slidably connected to the partition (12), and a pressing plate (13) is fixed to one end of the piston rod (94) away from the piston (93).

5. The tumor stem cell identification device according to claim 4, characterized in that: A spring (14) is sleeved on the outer side of the piston rod (94), and one end of the spring (14) abuts against the partition plate (12), while the other end of the spring (14) abuts against the pressing plate (13).

6. The tumor stem cell identification device according to claim 5, characterized in that: The control component comprises a transparent sleeve (15) threadedly connected to the transparent tube (91), and a dosage line (16) is arranged on the outside of the transparent sleeve (15).

7. A tumor stem cell identification device according to claim 6, characterized in that: The diameter of the pressing plate (13) is greater than the diameter of the transparent sleeve (15).

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