Rapid nucleic acid extraction device

Abstract

The utility model belongs to the technical field of nucleic acid extraction, and discloses a rapid nucleic acid extraction device which comprises a main cylinder, a needle tube is connected to one end of the main cylinder, scale marks are engraved on the outer side of the main cylinder, a push-pull rod is arranged in the main cylinder, a sealing rubber plug is mounted at one end of the push-pull rod, and a push-pull plate is mounted at the other end of the push-pull rod. The top of the main cylinder is connected with two symmetrical positioning rods, the outer walls of the two positioning rods are each provided with a plurality of sets of positioning grooves at equal intervals, the bottom ends of the two positioning rods are each provided with a limiting plate, and the top of the main cylinder is rotationally connected with a positioning assembly located outside the two positioning rods; the operation modes of the two positioning assemblies are the same, and when the two positioning rods are fixed, the position of the push-pull plate can be fixed; according to the structure, through the arrangement of the two positioning assemblies, the phenomenon that liquid leaks due to the fact that a worker mistakenly touches the push-pull plate can be prevented, the safety after liquid extraction is improved, and the adjusting mode is simple and convenient.

Description

Technical Field

[0001] The present application relates to the technical field of nucleic acid extraction, and more specifically, to a rapid nucleic acid extraction device. Background Art

[0002] Nucleic acid is the carrier of genetic information, the most important bioinformatics molecule, and the main object of molecular biology research. With the rapid development of molecular biology technology, the research and analysis of nucleic acid is being promoted and applied in the fields of clinical diagnosis, environmental testing, food safety testing, etc., and plays a great role. Since the magnetic bead method for extracting nucleic acid is simple to operate and takes a short time; it does not use toxic reagents such as benzene and chloroform in traditional methods, and is safe and non-toxic, it fully conforms to the modern environmental protection concept; the specific binding with nucleic acid makes the extracted nucleic acid high in purity and concentration, so the magnetic bead method for extracting nucleic acid is increasingly valued. The extraction steps of nucleic acid by magnetic bead method mainly include: (1) lysis; (2) binding; (3) washing; (4) elution; cell or tissue samples are lysed in the lysis solution, and the nucleic acid molecules released from the sample are specifically adsorbed to the surface of the magnetic particles, while impurities such as proteins are not adsorbed and remain in the solution. At this time, the surface-modified superparamagnetic silica nanoparticles "specifically bind" to the nucleic acid to form a "nucleic acid-magnetic bead complex"; then, under the action of an external magnetic field, the complex is separated. Finally, the non-specifically adsorbed impurities are washed away with elution solution, and after desalting and purification, the nucleic acid material to be extracted is obtained.

[0003] Some existing nucleic acid extraction devices are unable to fix the extraction devices when extracting some liquids. During operation, the staff may accidentally touch the nucleic acid extraction devices, causing liquid leakage and possible cross contamination.

[0004] In order to solve the above problems, the present application provides a rapid nucleic acid extraction device. Utility Model Content

[0005] The rapid nucleic acid extraction device provided in this application adopts the following technical solution:

[0006] A rapid nucleic acid extraction device comprises a main cylinder, one end of which is connected to a needle tube, the outer side of which is engraved with scale lines, a push-pull rod is arranged inside the main cylinder, a sealing rubber plug is installed at one end of the push-pull rod, a push-pull plate is installed at the other end of the push-pull rod, two symmetrical positioning rods are connected to the top of the main cylinder, the outer walls of the two positioning rods are provided with a plurality of groups of positioning grooves with equal distances, the bottom ends of the two positioning rods are installed with limit plates, and the top of the main cylinder and outside the two positioning rods are rotatably connected with a positioning assembly;

[0007] Through the above technical solution, the two positioning rods are fixed by the positioning assembly, and after the positioning rods are fixed, the push-pull plate can be fixed.

[0008] The positioning assembly includes a connecting cylinder rotatably connected to the top of the main cylinder body, a through groove is opened at the inner center of the connecting cylinder, a mounting cylinder is installed inside the connecting cylinder, a groove is opened inside the mounting cylinder, a connecting rod is connected to the inside of the mounting cylinder and at the position of the groove, a positioning block is installed at one end of the connecting rod, a connecting plate is installed on the outside of the connecting cylinder at the other end of the connecting rod, a pull ring is installed on the outside of the connecting plate, a spring is installed on one side of the inner side of the groove and on the outside of the connecting rod, and one end of the spring is connected to the positioning block.

[0009] Furthermore, the outer wall of the sealing rubber plug fits with the inner wall of the main cylinder, and the push-pull plate is located on the top of the main cylinder.

[0010] Furthermore, the diameters of the sealing rubber plug and the push-pull plate are both larger than the diameter of the push-pull rod, and the diameter of the push-pull plate is larger than the diameter of the sealing rubber plug.

[0011] Furthermore, through holes are provided inside the main cylinder at positions corresponding to the two positioning rods, and the diameter of the limiting plate is larger than the diameter of the positioning rods.

[0012] Through the above technical solution, the diameter of the limiting plate is larger than the diameter of the positioning rod, thereby preventing the positioning rod from being separated from the main cylinder.

[0013] Furthermore, a rotation groove is provided at the top of the main cylinder and outside the two positioning rods, and a rotation plate is installed at the bottom edge of the connecting cylinder.

[0014] Furthermore, the rotating plate is located inside the rotating groove, and the positioning rod is located inside the through groove.

[0015] Through the above technical solution, the connection cylinder is rotatably connected to the top of the main cylinder body and is located outside the positioning rod through the arrangement of the rotation plate and the rotation groove.

[0016] Furthermore, the diameter of the connecting plate is greater than the diameter of the connecting rod, the positioning block is located inside the groove, and the positioning block is adapted to the positioning groove.

[0017] Through the above technical solution, the positioning block can be located in the positioning groove at any position. When the positioning block is located in the positioning groove, the positioning rod can be fixed in the through groove.

[0018] In summary, this application includes the following beneficial technical effects:

[0019] When the nucleic acid extraction is completed, the user first operates a positioning component, holding the pull ring and pulling it outwards. When the positioning block is completely located inside the groove, the user rotates the connecting tube until the positioning block corresponds to the positioning groove at the appropriate position. The user releases the pull ring. At this time, the positioning block is driven to rebound forward and return to its original position through the setting of the spring. When the positioning block is located in the positioning groove at the appropriate position, the positioning rod can be fixed. The two positioning components operate in the same way. When both positioning rods are fixed, the position of the push-pull plate can be fixed. This structure can prevent the staff from accidentally touching the push-pull plate and causing liquid leakage, thereby improving the safety after liquid extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic diagram of the overall structure of this application;

[0021] Figure 2 A semi-exploded diagram of the structure of this application;

[0022] Figure 3 For this application Figure 2 Enlarged view of point A in the middle;

[0023] Figure 4 This is a schematic diagram of the positioning component of this application;

[0024] Figure 5 This is an internal diagram of the positioning component of this application;

[0025] Figure 6 This is a schematic diagram of the magnetic bead method for nucleic acid extraction of the present application.

[0026] Description of the numbers in the figure:

[0027] 1. Main cylinder; 2. Needle tube; 3. Scale line; 4. Push-pull rod; 5. Sealing rubber plug; 6. Push-pull plate; 7. Positioning rod; 8. Positioning groove; 9. Limit plate; 10. Rotating groove; 11. Positioning assembly; 111. Connecting cylinder; 112. Through groove; 113. Rotating plate; 114. Mounting cylinder; 115. Groove; 116. Connecting rod; 117. Positioning block; 118. Connecting plate; 119. Pull ring; 1110. Spring; 12. Single-row connecting tube; 121. Elution chamber; 122. Low-salt washing liquid chamber; 124. High-salt washing liquid chamber; 125. Spare chamber; 126. Lysis chamber; 127. Magnetic rod; 129. Transparent magnetic sleeve. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application; obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments, and all other embodiments obtained by ordinary technicians in this field based on the embodiments in the present application without making creative work are within the scope of protection of the present application.

[0029] In the description of the present application, it should be noted that the terms "upper", "lower", "inner", "outer", "top / bottom" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific position, and therefore cannot be understood as limiting the present application. In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0030] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "set / connected", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0031] Example:

[0032] The present application discloses a rapid nucleic acid extraction device. Figure 1 , Figure 2 and Figure 3 , including a main cylinder 1, one end of the main cylinder 1 is connected to a needle tube 2, the outer side of the main cylinder 1 is engraved with a scale line 3, the inside of the main cylinder 1 is provided with a push-pull rod 4, one end of the push-pull rod 4 is installed with a sealing rubber plug 5, the outer wall of the sealing rubber plug 5 is in contact with the inner wall of the main cylinder 1, the other end of the push-pull rod 4 is installed with a push-pull plate 6, the push-pull plate 6 is located at the top of the main cylinder 1, the diameters of the sealing rubber plug 5 and the push-pull plate 6 are both larger than the diameter of the push-pull rod 4, the diameter of the push-pull plate 6 is larger than the diameter of the sealing rubber plug 5, the main cylinder 1 is connected to the top of two symmetrical positioning rods 7, through holes are provided inside the main cylinder 1 and at positions corresponding to the two positioning rods 7, a plurality of groups of positioning grooves 8 with equal distances are provided on the outer walls of the two positioning rods 7, and a limiting plate 9 is installed at the bottom end of the two positioning rods 7, and the diameter of the limiting plate 9 is larger than the diameter of the positioning rod 7, a rotating groove 10 is provided at the top of the main cylinder 1 and outside the two positioning rods 7, and a positioning assembly 11 is rotatably connected to the top of the main cylinder 1 and outside the two positioning rods 7;

[0033] The setting of the scale line 3 facilitates the user to extract the nucleic acid to the required volume. When the push-pull plate 6 moves, the two positioning rods 7 are also driven to move. The positioning rods 7 can be fixed by the setting of the positioning assembly 11, and the push-pull plate 6 can be fixed at this time;

[0034] See also Figure 4 and Figure 5 The positioning assembly 11 includes a connecting cylinder 111 rotatably connected to the top of the main cylinder body 1, a through slot 112 is provided at the inner center of the connecting cylinder 111, the positioning rod 7 is located inside the through slot 112, a rotating plate 113 is installed at the bottom edge of the connecting cylinder 111, the rotating plate 113 is located inside the rotating slot 10, a mounting cylinder 114 is installed inside the connecting cylinder 111, a groove 115 is provided inside the mounting cylinder 114, a connecting rod 116 is connected to the inner part of the mounting cylinder 114 and located at the position of the groove 115, and the connecting rod 116 is connected to the connecting rod 116. A positioning block 117 is installed at one end of the groove 115, and the positioning block 117 is adapted to the positioning groove 8. The other end of the connecting rod 116 penetrates and extends to the outside of the mounting tube 114 and is installed with a connecting plate 118. The diameter of the connecting plate 118 is larger than the diameter of the connecting rod 116. A pull ring 119 is installed on the outside of the connecting plate 118. A spring 1110 is installed on one side of the inner side of the groove 115 and located outside the connecting rod 116. One end of the spring 1110 is connected to the positioning block 117.

[0035] The pull ring 119 is semicircular, which is convenient for the user to pull. The connecting rod 116 is located inside the installation cylinder 114 for sliding. The positioning block 117 can be engaged in the positioning groove 8 at different positions. The positioning assembly 11 is rotatably connected to the top of the main cylinder body 1 through the setting of the rotating plate 113 and the rotating groove 10. When the positioning rod 7 is not fixed, the connecting cylinder 111 is rotated to one side to make the positioning block 117 out of the position of the positioning groove 8, which is convenient for moving the position of the positioning rod 7.

[0036] See also Figure 6 The magnetic bead method nucleic acid extraction device comprises a single-row tube 12, a magnetic rod 127, and a transparent magnetic sleeve 129; the single-row tube 12 is provided with a lysis chamber 126 and a plurality of functional chambers, the functional chambers comprising a high-salt washing chamber 124, a low-salt washing chamber 122, an elution chamber 121, and a spare chamber 125; the nucleic acid extraction device is made of colorless and transparent materials for easy observation during operation, and is simple in design, lightweight, and easy to use;

[0037] The method of using the magnetic rod method in nucleic acid extraction and purification is as follows:

[0038] Mixing: The magnetic beads are pre-stored in the low-salt washing solution in the low-salt washing solution chamber 122, and the magnetic beads are placed in the chamber and mixed before use.

[0039] Lysis: Put the magnetic rod 127 into the transparent magnetic sleeve 129, absorb the magnetic beads in the low-salt washing liquid chamber 122 and inject them into the lysis chamber 126, and vibrate up and down to mix the sample to be tested, lysis solution and magnetic beads in the lysis chamber 126 to fully lyse.

[0040] Combination: the transparent magnetic sleeve 129 is moved out of the lysis chamber 126 of the single-row tube 12, and the magnetic rod 127 is inserted into the transparent magnetic sleeve 129; then, the transparent magnetic sleeve 129 and the magnetic rod 127 are synchronously inserted into the bottom of the lysis chamber 126 to adsorb the magnetic beads adsorbed with nucleic acid in the lysis chamber 126.

[0041] Washing: The magnetic beads adsorbed with nucleic acid are transferred to the high salt washing solution in the high salt washing solution chamber 124 and the low salt washing solution in the low salt washing solution chamber 122 in sequence for washing.

[0042] Elution: put it into the low-salt washing liquid chamber 122 for adsorption together with the magnetic rod 127, and transfer it to the elution chamber 121 for elution;

[0043] pass Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5 The structure in the middle facilitates the extraction of liquid in the single-row connecting tube 12, thereby improving the extraction efficiency of the liquid.

[0044] The implementation principle of this embodiment is as follows: when extracting liquid, the user holds the push-pull plate 6 to drive the push-pull rod 4 and the sealing rubber plug 5 set at the bottom to move, and the push-pull plate 6 drives the two positioning rods 7 to move at the same time, and the needle tube 2 extracts liquid. The setting of the scale line 3 facilitates the user to observe the volume of the extracted liquid; in order to prevent the staff from accidentally touching the push-pull plate 6 after the liquid is extracted and causing the liquid to leak, the two positioning components 11 can be set to avoid such a phenomenon;

[0045] When the liquid extraction is finished, the user first operates a positioning component 11, and the position of the hand-held pull ring 119 drives the connecting plate 118 to be pulled outward, and the connecting plate 118 drives the positioning block 117 to move through the connecting rod 116. At this time, the spring 1110 is in an elongated state. When the positioning block 117 is completely located inside the groove 115, the user rotates the connecting tube 111 until the positioning block 117 corresponds to the positioning groove 8 at a suitable position on the outer side of the positioning rod 7. The user releases the pull ring 119. At this time, the positioning block 117 is driven to rebound forward and return to its original position through the setting of the spring 1110. When the positioning block 117 is located in the positioning groove 8 at a suitable position, the positioning rod 7 can be fixed. At this time, another positioning component 11 is operated in the same step as above. When both positioning rods 7 are fixed, the position of the push-pull plate 6 can be fixed.

[0046] When it is necessary to press the push-pull plate 6 later, the user first operates a positioning component 11, holds the pull ring 119 and pulls it outward. When the positioning block 117 is completely located inside the groove 115, the user rotates the connecting tube 111 to disengage the positioning block 117 from the position of the positioning groove 8. The operation method of the two positioning components 11 is the same. When the two positioning blocks 117 are disengaged from the position of the positioning groove 8, the push-pull plate 6 can be moved.

[0047] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A rapid nucleic acid extraction device, comprising a main cylinder (1), characterized in that: One end of the main cylinder (1) is connected to a needle tube (2), the outer side of the main cylinder (1) is engraved with a scale line (3), a push-pull rod (4) is arranged inside the main cylinder (1), one end of the push-pull rod (4) is installed with a sealing rubber plug (5), the other end of the push-pull rod (4) is installed with a push-pull plate (6), the top of the main cylinder (1) is connected to two symmetrical positioning rods (7), the outer walls of the two positioning rods (7) are provided with a plurality of groups of equidistant positioning grooves (8), the bottom ends of the two positioning rods (7) are installed with a limit plate (9), and the top of the main cylinder (1) and the outside of the two positioning rods (7) are rotatably connected with a positioning assembly (11); The positioning assembly (11) comprises a connecting cylinder (111) rotatably connected to the top of the main cylinder (1), a through groove (112) is provided at the inner center of the connecting cylinder (111), a mounting cylinder (114) is installed inside the connecting cylinder (111), a groove (115) is provided inside the mounting cylinder (114), a connecting rod (116) is connected inside the mounting cylinder (114) and at a position located at the groove (115), a positioning block (117) is installed at one end of the connecting rod (116), a connecting plate (118) is installed at the other end of the connecting rod (116) that passes through and extends to the outside of the mounting cylinder (114), a pull ring (119) is installed on the outside of the connecting plate (118), a spring (1110) is installed on one side of the inner side of the groove (115) and located outside the connecting rod (116), and one end of the spring (1110) is connected to the positioning block (117).

2. A rapid nucleic acid extraction device according to claim 1, characterized in that: The outer wall of the sealing rubber plug (5) fits the inner wall of the main cylinder (1), and the push-pull plate (6) is located at the top of the main cylinder (1).

3. A rapid nucleic acid extraction device according to claim 1, characterized in that: The diameters of the sealing rubber plug (5) and the push-pull plate (6) are both larger than the diameter of the push-pull rod (4), and the diameter of the push-pull plate (6) is larger than the diameter of the sealing rubber plug (5).

4. A rapid nucleic acid extraction device according to claim 1, characterized in that: Through holes are provided inside the main cylinder (1) and at positions corresponding to the two positioning rods (7), and the diameter of the limiting plate (9) is greater than the diameter of the positioning rods (7).

5. A rapid nucleic acid extraction device according to claim 1, characterized in that: A rotation groove (10) is provided at the top of the main cylinder (1) and outside the two positioning rods (7), and a rotation plate (113) is installed at the bottom edge of the connecting cylinder (111).

6. A rapid nucleic acid extraction device according to claim 5, characterized in that: The rotating plate (113) is located inside the rotating groove (10), and the positioning rod (7) is located inside the through groove (112).

7. A rapid nucleic acid extraction device according to claim 1, characterized in that: The diameter of the connecting plate (118) is greater than the diameter of the connecting rod (116), the positioning block (117) is located inside the groove (115), and the positioning block (117) is matched with the positioning groove (8).

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