Adeno-associated virus purification kit

Abstract

The utility model discloses an adeno-associated virus purification kit, relates to the technical field of kits, and aims to solve the problems that an existing kit is single in structure, lacks a reagent tube pinhole plugging structure and an anti-leakage function, and is insufficient in improving the convenience of extracting a solution in a reagent tube. According to the technical scheme, the kit is characterized by comprising a kit body, an inner supporting base is installed in the kit body in a buckled mode, a foam inserting plate adheres to the top end face of the interior of the inner supporting base, inserting holes corresponding in position are formed in the upper surface of the foam inserting plate and the end face of the inner supporting base, and reagent bottles and reagent tubes are inserted into the inserting holes; a rubber block is adhered to the outer surface of the reagent tube, and a packaging bag covers the bottom opening of the inner supporting seat. The effects that the solution in the reagent tube can be effectively prevented from leaking from the needle hole, and meanwhile, the solution in the reagent tube can be conveniently extracted are achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of reagent kits, in particular to an adeno-associated virus purification reagent kit. Background Art

[0002] In recent years, significant breakthroughs have been made in gene editing technology, and cell therapy and gene therapy have become treatment means for many diseases that were previously untreatable or difficult to treat. Adeno-associated virus vectors are one of the most commonly used virus vectors in the field of gene therapy, and their application potential has been verified in a large number of clinical and preclinical experiments. A series of operations are required when using adeno-associated viruses, including purification operations, and relevant reagent kits are needed in this process.

[0003] The existing reagent kits have a single structure, lack a reagent tube pinhole plugging structure and a leakage prevention function, and are insufficient in improving the convenience of extracting the solution inside the reagent tube. Content of the Utility Model

[0004] The purpose of the utility model is to provide an adeno-associated virus purification reagent kit that can effectively prevent the solution inside the reagent tube from leaking from the pinhole, and at the same time can conveniently perform the operation of extracting the solution inside the reagent tube.

[0005] To achieve the above purpose, the utility model provides the following technical solutions:

[0006] An adeno-associated virus purification reagent kit, including a reagent kit body, an inner support seat is snap-fitted inside the reagent kit body, a foam insertion board is adhered to the inner top surface of the inner support seat, corresponding jacks are provided on the upper surface of the foam insertion board and the end surface of the inner support seat, and a reagent bottle and a reagent tube are inserted into the jacks. A rubber block is pasted on the outer surface of the reagent tube, and a packaging bag is buckled over the bottom opening of the inner support seat.

[0007] By adopting the above technical solution, it can effectively avoid the leakage of the solution when extracting the solution inside the reagent tube.

[0008] Further, a snap groove is provided on the side surface of the inner support seat, an elastic cord is provided at the opening of the packaging bag, and the elastic cord and the opening of the packaging bag are snapped inside the snap groove.

[0009] By adopting the above technical solution, the position stability of the packaging bag can be improved.

[0010] Further, a retaining ring is fixedly connected to the upper edge position of the reagent tube.

[0011] By adopting the above technical solution, the retaining ring can be used to provide support for the placement of the reagent tube.

[0012] Further, one side edge of the upper opening of the kit body is hingedly connected with a box cover, and a support bar is integrally connected to the edge of the box cover.

[0013] By adopting the above technical solution, the support bar can be used to keep the box cover in a vertical state.

[0014] Further, a placement rack is rotatably connected to the inner wall of the box cover, and one end of the placement rack is provided with an L-shaped structure.

[0015] By adopting the above technical solution, it is convenient to flip the placement rack and at the same time enable the placement rack to be placed horizontally.

[0016] Further, a rubber foot is respectively arranged at each of the four corner positions of the bottom end face of the box cover, and a locking component that cooperates with each other is arranged between the box cover and the kit body.

[0017] By adopting the above technical solution, the box cover can be firmly buckled at the opening of the kit body.

[0018] In summary, the beneficial technical effects of the present utility model are as follows:

[0019] 1. The present utility model can use a reagent tube for purification operation. When extracting the solution inside the reagent tube in the later stage of purification, the syringe needle pierces through the rubber block and is placed inside the reagent tube, and then the solution inside the reagent tube is extracted. During this process, the rubber block can be used to timely block the needle hole in the reagent tube, and avoid the solution in the reagent tube from leaking out of the needle hole during the interval of extracting the solution, effectively improving the overall stability. And when extracting the solution inside the reagent tube, the inner support seat is inverted so that the lower opening of the inner support seat faces upward. Since a packaging bag is wrapped at the lower opening of the inner support seat, the packaging bag can be placed below the reagent tube to avoid the solution extracted from the reagent tube from dripping on the tabletop, and at the same time it is also convenient to hold various consumables and instruments, effectively improving the safety of the adeno-associated virus purification operation;

[0020] 2. The present utility model can flip the box cover, use the support bar to keep the box cover in an upright state, and then flip the placement rack, which is convenient for inserting and placing the reagent tube, thereby providing convenience for extracting the solution inside the reagent tube. Description of the Drawings

[0021] Figure 1 is the first perspective view of the three-dimensional structure of the present utility model;

[0022] Figure 2 is the second perspective view of the three-dimensional structure of the present utility model.

[0023] In the figure: 1. Kit body; 2. Lid; 3. Placement rack; 4. Inner support base; 5. Placement groove; 6. Buckle groove; 7. Sealing bag; 8. Support bar; 9. Rubber feet; 10. Reagent tube; 11. Rubber block; 12. Retaining ring; 13. Foam insert. Detailed implementation

[0024] The following further elaborates on the method of the present utility model in conjunction with the attached drawings.

[0025] Refer to the attached Figure 1 and the attached Figure 2 , an adeno-associated virus purification kit, including a kit body 1, an inner support base 4 is snap-mounted inside the kit body 1, a foam insert 13 is adhered to the inner top surface of the inner support base 4, corresponding jacks are provided on the upper surface of the foam insert 13 and the end surface of the inner support base 4, and reagent bottles and reagent tubes 10 are inserted into the jacks. A rubber block 11 is pasted on the outer surface of the reagent tube 10. The bottom opening of the inner support base 4 is covered with a sealing bag 7. A buckle groove 6 is provided on the side surface of the inner support base 4. An elastic cord is provided at the opening of the sealing bag 7, and the elastic cord and the opening of the sealing bag 7 are buckled inside the buckle groove 6. Among them, the purification operation can be carried out using the reagent tube 10. When extracting the solution inside the reagent tube 10 in the later stage of purification, the syringe needle pierces through the rubber block 11 and is placed inside the reagent tube 10, and then the solution inside the reagent tube 10 is extracted. During this process, the rubber block 11 can timely block the needle hole in the reagent tube 10, and avoid the solution in the reagent tube 10 from leaking out through the needle hole during the gap of extracting the solution, effectively improving the overall stability. And when extracting the solution inside the reagent tube 10, invert the inner support base 4 so that the lower opening of the inner support base 4 faces upward. Since the sealing bag 7 is wrapped around the lower opening of the inner support base 4, the sealing bag 7 can be placed below the reagent tube 10, avoiding the solution extracted from the reagent tube 10 from dripping onto the tabletop, and at the same time facilitating the loading of various consumables and instruments, effectively improving the safety of the adeno-associated virus purification operation.

[0026] Refer to Figure 1 and Figure 2 , a retaining ring 12 is fixedly connected to the upper edge position of the reagent tube 10. One side edge of the upper opening of the kit body 1 is hingedly connected to a lid 2. A support bar 8 is integrally connected to the edge of the lid 2. A placement rack 3 is rotatably connected to the inner wall of the lid 2. One end of the placement rack 3 is provided with an L-shaped structure. Among them, the lid 2 can be flipped, and the support bar 8 is used to keep the lid 2 in an upright state. Then the placement rack 3 is flipped to facilitate the insertion and placement of the reagent tube 10, thereby providing convenience for extracting the solution inside the reagent tube 10.

[0027] Refer to Figure 2, a rubber foot 9 is respectively arranged at each of the four corner positions of the bottom end face of the box cover 2, and a locking component which cooperates with each other is arranged between the box cover 2 and the reagent kit body 1. The rubber foot 9 can be used to improve the placement stability of the reagent kit body 1.

[0028] Working principle: During use, open the locking component on the box cover 2 and the reagent kit body 1, then flip the box cover 2. Under the limitation of the support bar 8, ensure that the box cover 2 is in a vertical state. Then take out the reagent tube 10 and the reagent bottle, and then perform the purification operation of the adeno-associated virus. After the solution is stratified in the reagent tube 10, flip the placement rack 3, and then insert the reagent tube 10 into the placement hole of the placement rack 3. At this time, the retaining ring 12 provides limit support for the reagent tube 10. Then invert the inner support seat 4 and place the inner support seat 4 inside the reagent kit body 1, so that the bottom opening of the inner support seat 4 faces upward. At this time, the encapsulation bag 7 wrapped around the bottom opening of the inner support seat 4 can be used to hold the accidental drips and medical consumables during the extraction of the solution. When extracting the solution inside the reagent tube 10, pierce the rubber block 11 with the syringe needle and place it inside the reagent tube 10, and then extract the solution inside the reagent tube 10. During this process, the rubber block 11 can be used to timely block the needle hole in the reagent tube 10, and avoid the solution in the reagent tube 10 from leaking out of the needle hole during the gap of extracting the solution. After the operation is completed, place all the waste objects inside the encapsulation bag 7, then take out the encapsulation bag 7 from the buckle groove 6, and then perform the bundling and sealing operation on the opening of the encapsulation bag 7.

[0029] The embodiments of this specific implementation manner are all preferred embodiments of the present invention, and do not limit the protection scope of the present invention accordingly. Therefore, all equivalent changes made according to the structure, shape, and principle of the present invention should be covered within the protection scope of the present invention.

Claims

1. An adeno-associated virus purification kit, comprising a kit body (1), characterized in that: An inner support seat (4) is installed inside the reagent kit body (1) by means of a snap fastener. A foam insertion board (13) is adhered to the inner top surface of the inner support seat (4). Corresponding jacks are provided on the upper surface of the foam insertion board (13) and on the end surface of the inner support seat (4), and reagent bottles and reagent tubes (10) are inserted into the jacks. A rubber block (11) is adhered to the outer surface of the reagent tube (10). A packaging bag (7) covers the bottom opening of the inner support seat (4).

2. The adeno-associated virus purification kit according to claim 1, wherein: A snap groove (6) is provided on the side surface of the inner support seat (4). An elastic cord is provided at the opening of the packaging bag (7), and the elastic cord and the opening of the packaging bag (7) are snapped inside the snap groove (6).

3. The adeno-associated virus purification kit according to claim 1, wherein: A retaining ring (12) is fixedly connected to the upper edge position of the reagent tube (10).

4. A recombinant adeno-associated virus purification kit according to claim 1, wherein: One side edge of the upper opening of the reagent kit body (1) is hinged to a box cover (2), and a support strip (8) is integrally connected to the edge of the box cover (2).

5. The adeno-associated virus purification kit according to claim 4, characterized in that: A placement rack (3) is rotatably connected to the inner wall of the box cover (2), and one end of the placement rack (3) is provided with an L-shaped structure.

6. The adeno-associated virus purification kit according to claim 4, wherein: A rubber support foot (9) is respectively provided at each of the four corner positions of the bottom end surface of the box cover (2). A locking component that cooperates with each other is provided between the box cover (2) and the reagent kit body (1).

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