Cooling device suitable for western blot film transfer
The modularly designed L-shaped water box structure solves the problems of temperature fluctuation and high cost in the cooling process of the Western blot transfer device, achieving stable and economical temperature control, which is suitable for Western blot experiments.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-27
- Publication Date
- 2026-04-03
AI Technical Summary
Existing Western blot transfer devices suffer from large temperature fluctuations, frequent maintenance, and high costs during the cooling process, making it difficult to achieve stable and economical temperature control.
A modular cooling device was designed, which adopts an L-shaped water box structure. Multiple L-shaped water boxes are slidably connected in the holding basin, which facilitates replacement and maintenance. It combines ice water mixture for cooling and is suitable for electrophoresis instrument components of different specifications.
It achieves stable temperature control, reduces equipment costs, improves ease of use and versatility, simplifies the maintenance process, and avoids protein denaturation or gel deformation.
Smart Images
Figure CN224072016U_ABST
Abstract
Description
Technical Field
[0001] This utility model mainly relates to the technical field of experimental equipment, specifically a cooling device suitable for Western blot membrane transfer. Background Technology
[0002] Western blot is a commonly used experimental method in molecular biology, biochemistry, and immunogenetics to detect the expression levels of specific proteins. The basic principle of Western blot involves the following steps: Protein separation: Proteins in the sample are separated according to their molecular weight using polyacrylamide gel electrophoresis (PAGE). Transfer: The separated proteins are transferred to a solid support (such as PVDF or nitrocellulose membrane) to immobilize them. Blocking: Non-specific binding sites on the membrane are blocked with a protein that does not contain the target protein (such as bovine serum albumin BSA or skim milk powder) to reduce background noise. Antibody incubation: The membrane is first incubated with a specific primary antibody to bind the target protein, and then a secondary antibody (usually labeled with an enzyme or fluorescent dye) is used to detect the target protein bound by the primary antibody. Signal detection: The bound secondary antibody is detected by chemiluminescence, fluorescence, or colorimetry to identify the target protein and analyze its expression level.
[0003] In the membrane transfer process, wet transfer (tank transfer) requires assembling a "sandwich" structure (filter paper-gel-membrane-filter paper). This structure is prone to overheating under high current, necessitating an additional cooling system (such as an ice bath or circulating cooling device). Otherwise, protein denaturation or gel deformation may occur. Existing cooling methods mainly include: 1. Ice bath or external cooling: using an ice-water mixture and regularly replacing the ice. The disadvantage is the need for frequent ice maintenance, and temperature fluctuations may affect transfer consistency. 2. Circulating cooling system: built-in or external cooling devices. The disadvantage is higher equipment cost and the need for regular maintenance of the circulating system.
[0004] In summary, designing a device with more stable temperature control, lower price, and simple assembly is a technical problem that urgently needs to be solved by those in this field. Utility Model Content
[0005] Therefore, the purpose of this utility model is to provide a cooling device suitable for western blot film transfer, so as to solve the technical problems mentioned in the background art.
[0006] To achieve the above objectives, this utility model provides the following technical solution:
[0007] A cooling device suitable for Western blot membrane transfer includes a container, the inner wall of which is symmetrically provided with U-shaped sides on the top axis, a limiting bracket is provided on the top of the U-shaped sides, multiple sets of L-shaped water boxes are inserted through the top of the limiting bracket, and an L-shaped top cover is provided at the corner of one end of the L-shaped water box.
[0008] Preferably, the outer wall of the container is provided with U-shaped handles at both ends.
[0009] Preferably, a linear groove is embedded on the side of the U-shaped side near the inner wall of the container.
[0010] Preferably, the limiting stop is provided with a side plate on one side, and a plurality of strip grooves are provided through the inner wall of one end of the limiting stop.
[0011] Preferably, the side plate is slidably connected to the inner wall of the linear groove.
[0012] Preferably, the inner walls of the plurality of strip grooves are slidably connected to the outer wall of the other end of the L-shaped water box, and the width of the strip grooves is the same as the width of the outer wall of the other end of the L-shaped water box.
[0013] Preferably, the L-shaped water box has a water inlet at the top of one corner, and a strip-shaped protrusion is provided on the outer wall of one corner.
[0014] Preferably, the outer wall of the strip-shaped protrusion is slidably connected to the inner wall of the bottom of the L-shaped top cover.
[0015] In summary, this technical solution has the following main advantages:
[0016] In this invention, each component adopts a modular design, which can be easily assembled for use in the laboratory. In addition, multiple L-shaped water boxes are provided, which can be easily replaced at any time during long electrophoresis experiments to maintain the internal temperature of the container and effectively prevent protein denaturation caused by excessive temperature.
[0017] The entire device has a simple structure, is easy to use, has low manufacturing and operating costs, can be used multiple times and in multiple cycles, has low maintenance costs, and is easy to use and promote. Attached Figure Description
[0018] Figure 1 This is an isometric view of the overall structure of this utility model;
[0019] Figure 2 This is a plan view of the overall structure of this utility model;
[0020] Figure 3 Axonometric view of part of the structure of this utility model Figure 1 ;
[0021] Figure 4Axonometric view of part of the structure of this utility model Figure 2 ;
[0022] Figure 5 This is an isometric drawing of the specific structure of the L-shaped water box of this utility model;
[0023] Figure 6 This is a schematic diagram of the assembly of the present invention with the electrophoresis apparatus components.
[0024] Figure descriptions: 10. Container body; 11. U-shaped side; 12. Limiting bracket; 13. L-shaped water box; 14. L-shaped top cover; 101. U-shaped handle; 111. Linear groove; 121. Side plate; 122. Strip groove; 131. Water inlet; 132. Strip protrusion. Detailed Implementation
[0025] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention.
[0026] Example
[0027] like Figure 1 and Figure 2 As shown, a cooling device suitable for Western blot transfer includes a container 10. The top of the inner wall of the container 10 is symmetrically provided with U-shaped sides 11. The top of the U-shaped sides 11 is provided with a limiting bracket 12. Multiple sets of L-shaped water boxes 13 are inserted through the top of the limiting bracket 12. An L-shaped top cover 14 is provided at the top of one corner of the L-shaped water box 13.
[0028] It should be noted that, in this embodiment, as Figure 3 and Figure 6 As shown, the outer walls of the container 10 are provided with U-shaped handles 101 at both ends. The U-shaped handles 101 are used to facilitate the movement of the container 10. In addition, when an electrophoresis apparatus is installed on the inner wall of the container 10, this device is installed to facilitate the movement of the experimenter and provide a convenient force point, thereby increasing the ease of use.
[0029] like Figure 3 As shown, a set of U-shaped sides 11 are provided on the inner wall of the container 10, and they are symmetrically arranged. The U-shaped sides 11 themselves can support and place the limiting bracket 12. The linear groove 111 embedded on the side of the U-shaped side 11 near the inner wall of the container 10 is a limiting structure. By inserting the bottom of the side plate 121 provided on one side of the limiting bracket 12 into the linear groove 111, the support and placement stability of the limiting bracket 12 is further improved. Secondly, the limiting bracket 12 with the side plate 121 has identification features. When using it, the experimenter can easily distinguish the front and back of the limiting bracket 12, which increases the ease of assembly.
[0030] The side plate 121 is slidably connected to the inner wall of the linear groove 111. The assembly nature of the limiting bracket 12 increases its storage capacity. When in use, the limiting bracket 12 can be removed and installed on the U-shaped side 11 of the holding basin 10. Importantly, this disassembly design allows for different specifications of the L-shaped water box 13 in this cooling device, making it suitable for electrophoresis apparatus components of different sizes and improving versatility.
[0031] like Figure 4 and Figure 5 As shown, a plurality of strip grooves 122 are provided on the inner wall of one end of the limiting bracket 12. The inner walls of the plurality of strip grooves 122 are slidably connected to the outer wall of the other end of the L-shaped water box 13, and the width of the strip grooves 122 is the same as the width of the outer wall of the other end of the L-shaped water box 13. The plurality of strip grooves 122 serve to limit and position the L-shaped water box 13. During the experimental operation, the L-shaped water box 13 can be easily and conveniently placed on the inner wall of the strip grooves 122 and placed in the inner cavity of the container 10, providing convenience for subsequent cooling use.
[0032] Two L-shaped water boxes 13 of the same specification can be used at a time. When using this device, a 2+2 usage scheme is adopted, that is, two are used for immediate assembly and cooling, and two are used as spares for replacement at any time. Before the Western blot experiment, a water inlet 131 is inserted through the top corner of one end of the L-shaped water box 13. Fill the L-shaped water box 13 with 90% of its inner volume with water, and place the L-shaped water box 13 in the refrigerator for freezing storage. It can be used as needed, and can be placed back in the refrigerator after use. Compared with the traditional method of directly using ice to pile up the electrophoresis apparatus components, this method increases the convenience of replacement and maintaining a stable temperature. It is only necessary to remove the L-shaped water box 13 and put in a new L-shaped water box 13.
[0033] The outer wall of one corner of the L-shaped water box 13 is provided with a strip protrusion 132. The outer wall of the strip protrusion 132 is slidably connected to the inner wall of the bottom of the L-shaped top cover 14. The strip protrusion 132 is used to limit and lock the L-shaped top cover 14. The L-shaped water box 13 does not need to be filled and drained repeatedly after one filling. When the laboratory regularly maintains this device, the equipment can be maintained by opening the L-shaped top cover 14.
[0034] It should be further noted that, in order to control manufacturing costs, all components of this device can be made entirely of plastic materials, and many components of this device adopt a modular design, which can be easily customized to different specifications. Compared with using expensive cooling equipment, this device has a lower operating cost.
[0035] The working principle of this utility model is as follows:
[0036] During the western blot transfer test, the limiting bracket 12 is installed on the top of the U-shaped side 11 of the container 10, and multiple sets of L-shaped water boxes 13 prepared in the refrigerator are inserted into the inner wall of multiple strip grooves 122 of the U-shaped side 11. The electrophoresis apparatus is placed in the center of the empty groove in the inner cavity of the container 10. Since the multiple sets of L-shaped water boxes 13 have occupied most of the space in the inner cavity of the container 10, a small amount of water is injected into the container 10 until the water level reaches a certain position on the outer wall of the electrophoresis apparatus to effectively wrap and cool the area.
[0037] When a small amount of injected water comes into contact with the frozen L-shaped water box 13, as the temperature decreases, the water near the outer wall of the electrophoresis apparatus component wraps around the outer wall of the electrophoresis apparatus component and forms an ice-water mixture, which increases the temperature conduction effect. The electrophoresis time of the western blot membrane transfer experiment is relatively long, and the spare L-shaped water box 13 can be replaced in the middle according to the actual situation to maintain the temperature transfer.
[0038] The above embodiments are only for illustrating the technical concept of this utility model and should not be construed as limiting the scope of protection of this utility model. Any modifications made to the technical solution based on the technical concept proposed by this utility model shall fall within the scope of protection of this utility model.
Claims
1. A cooling device suitable for western blot membrane transfer, characterized in that The utility model relates to a kind of water storage pots, including containing basin (10), the inside wall top axis symmetry of containing basin (10) is equipped with U-shaped side (11), the top of U-shaped side (11) is equipped with limiting baffle (12), multiple groups L-shaped water box (13) are passed through and are equipped in the top of limiting baffle (12), L-shaped top cover (14) is equipped in the top of one end corner of L-shaped water box (13).
2. The cooling device for western blot membrane transfer according to claim 1, characterized in that, The outer wall of the containing basin (10) is provided with a U-shaped handle (101) at both ends.
3. The cooling device for western blot membrane transfer according to claim 1, characterized in that, The U-shaped side (11) is embedded with a linear groove (111) near the inner wall of the containing basin (10).
4. The cooling device for western blot membrane transfer according to claim 3, characterized in that, The limiting baffle (12) is provided with a side plate (121) on one side, and a plurality of strip grooves (122) are provided in the inner wall of one end of the limiting baffle (12).
5. The cooling device for western blot membrane transfer according to claim 4, characterized in that, The side plate (121) is in sliding connection with the inner wall of the linear groove (111).
6. The cooling device for western blot membrane transfer according to claim 4, characterized in that, The inner wall of the plurality of strip grooves (122) is in sliding connection with the outer wall of the other end of the L-shaped water box (13), and the width of the strip groove (122) is the same as the width of the outer wall of the other end of the L-shaped water box (13).
7. The cooling device for western blot membrane transfer according to claim 1, characterized in that, The L-shaped water box (13) is provided with a water inlet (131) at the top of one end corner, and a strip protrusion (132) is provided on the outer wall of one end corner of the L-shaped water box (13).
8. The cooling device for western blot membrane transfer according to claim 7, characterized in that, The outer wall of the strip protrusion (132) is in sliding connection with the bottom inner wall of the L-shaped top cover (14).