Cell treatment consumable pipe group and cell treatment equipment

Abstract

The utility model provides a cell treatment consumable pipe group and cell treatment equipment. The cell treatment consumable pipe group comprises a centrifugal container, a first pipe group, a second pipe group, a first communicating pipeline, a magnetic separation column, a first separation pipeline and a second separation pipeline, one end of the third process first communicating pipeline communicates with the third process first pipe group and is located at a first communicating point, and the other end of the third process first communicating pipeline communicates with the third process second pipe group and is located at a second communicating point; the third process first separation pipeline, the third process magnetic separation column and the third process second separation pipeline are sequentially communicated, and the end, away from the third process magnetic separation column, of the third process first separation pipeline is communicated to the third process first pipe set and located at a third communication point. The end, away from the third process magnetic separation column, of the third process second separation pipeline communicates with the third process first pipe set and is located at a fourth communication point. According to the utility model, the pollution of cells is avoided, and the cell treatment efficiency is improved.

Description

Technical Field

[0001] This utility model belongs to the field of cell processing technology, and in particular relates to a cell processing consumable tube assembly and cell processing equipment. Background Technology

[0002] Currently, in the field of cell therapy, cell processing generally involves multiple steps, such as PBMC (peripheral blood mononuclear cell) isolation, magnetic bead incubation, cell sorting, cell harvesting, and cell culture. In existing technologies, each of these steps requires operators to change different equipment or tubing separately, resulting in cumbersome processes and low efficiency. Furthermore, changing equipment can easily lead to sample contamination. Summary of the Invention

[0003] This invention addresses the technical problems of low cell processing efficiency and easy contamination in the prior art by providing a cell processing consumable tube assembly and cell processing equipment.

[0004] In view of the above technical problems, this utility model provides a cell processing consumable tubing assembly, including a centrifuge container, a first tubing assembly connected to the centrifuge container and used for matching a first drive pump, a second tubing assembly connected to the centrifuge container and used for matching a second drive pump, a first connecting pipeline, a magnetic sorting column for sorting cells labeled with magnetic beads by magnetic adsorption, a first sorting pipeline, and a second sorting pipeline used for matching a third drive pump.

[0005] One end of the first connecting pipe is connected to the first pipe group and located at the first connecting point, and the other end of the first connecting pipe is connected to the second pipe group and located at the second connecting point; the first connecting point and the centrifuge container are respectively located on opposite sides of the first driving pump, and the second connecting point and the centrifuge container are respectively located on opposite sides of the second driving pump; the first sorting pipe, the magnetic sorting column and the second sorting pipe are connected in sequence, the end of the first sorting pipe away from the magnetic sorting column is connected to the first pipe group and located at the third connecting point, and the end of the second sorting pipe away from the magnetic sorting column is connected to the first pipe group and located at the fourth connecting point, and the third connecting point and the fourth connecting point are respectively located on opposite sides of the first driving pump.

[0006] Optionally, the cell treatment consumable tubing assembly further includes a second connecting tubing and a third connecting tubing; one end of the second connecting tubing is connected to the second tubing assembly and located at a fifth connecting point, and the other end of the second connecting tubing is connected to the second tubing assembly and located at a sixth connecting point; the fifth connecting point and the sixth connecting point are respectively located on opposite sides of the second drive pump;

[0007] One end of the third connecting pipe is connected to the first pipe group and is located at the seventh connecting point; the other end of the second connecting pipe is connected to the second pipe group and is located at the eighth connecting point; the seventh connecting point is located between the first driving pump and the centrifuge container, and the eighth connecting point is located between the second driving pump and the centrifuge container.

[0008] Optionally, the cell processing consumable tubing assembly further includes a first process tubing for connecting to a first process solution container; one end of the first process tubing away from the first process solution container is connected to the second sorting tubing and located at a ninth connection point; the ninth connection point is located between the third drive pump and the fourth connection point.

[0009] Optionally, the first sorting pipeline is provided with a fluid filter for filtering large particulate impurities and a separation column for adsorbing some cells; the fluid filter is located between the third connection point and the separation column.

[0010] Optionally, the cell processing consumable tubing assembly further includes a first waste liquid line for connecting to a first waste liquid container; one end of the waste liquid line away from the first waste liquid container is connected to the first sorting line and located at the tenth connection point; the tenth connection point is located between the separation column and the magnetic sorting column.

[0011] Optionally, the cell processing consumable tubing assembly further includes a fourth connecting tubing; one end of the fourth connecting tubing is connected to the first sorting tubing and located at the eleventh connecting point, and the other end of the fourth connecting tubing is connected to the second sorting tubing and located at the twelfth connecting point; the eleventh connecting point is located between the magnetic sorting column and the tenth connecting point, and the twelfth connecting point is located between the third drive pump and the fourth connecting point; and / or

[0012] The cell treatment consumable tubing assembly further includes a fifth connecting tubing; one end of the fifth connecting tubing is connected to the first sorting tubing and located at the thirteenth connecting point, and the other end of the fifth connecting tubing is connected to the first waste liquid tubing; the thirteenth connecting point is located between the third connecting point and the fluid filter; and / or

[0013] The cell treatment consumable tubing assembly also includes a sixth connecting tubing; one end of the sixth connecting tubing is connected to the first waste liquid tubing, and the other end of the sixth connecting tubing is connected to the first process tubing.

[0014] Optionally, the cell processing consumable tubing assembly further includes a second waste liquid line for connecting to a second waste liquid container; one end of the second waste liquid line away from the second waste liquid container is connected to the second sorting line and located at the fourteenth connection point; the fourteenth connection point is located between the third drive pump and the fourth connection point.

[0015] Optionally, the first tubing assembly includes a first centrifugal line for matching the first drive pump, a second process line for connecting to a second process solution container, and a first transfer line for connecting to a first transfer liquid container; one end of the first centrifugal line is connected to the centrifugal container, and the other end of the first centrifugal line is connected to the end of the second process line away from the second process solution container; the end of the first transfer line away from the first transfer liquid container is connected to the second process line.

[0016] Optionally, the second tubing assembly includes a second centrifugal line for matching the second drive pump, and a third process line for connecting to a third process solution container; one end of the second centrifugal line is connected to the centrifugal container, and the other end of the second centrifugal line is connected to the end of the third process line away from the third process solution container;

[0017] The second tubing set further includes a sample tubing for connecting to a sample container; one end of the second centrifuge tubing away from the centrifuge container is connected to the other end of the sample tubing away from the sample container; and / or

[0018] The second tubing assembly further includes a third waste liquid line for connecting to a third waste liquid container; one end of the second centrifuge line away from the centrifuge container is connected to the end of the third waste liquid line away from the third waste liquid container; and / or

[0019] The second pipe assembly further includes a harvesting pipe for connecting to a harvesting container, wherein the end of the second centrifugal pipe away from the centrifugal container is connected to the end of the harvesting pipe away from the harvesting container; and / or

[0020] The second tubing assembly also includes a second transfer line for connecting to the second transfer liquid container; one end of the second transfer line away from the second transfer liquid container is connected to the third process line.

[0021] A cell processing device includes a first drive pump, a second drive pump, a third drive pump, at least one first switching valve for controlling the on / off state of a pipeline in a first pipeline group, at least one second switching valve for controlling the on / off state of a pipeline in a second pipeline group, a third switching valve for controlling the on / off state of a first connecting pipeline, a fourth switching valve for controlling the on / off state of a first sorting pipeline, a fifth switching valve for controlling the on / off state of a second sorting pipeline, and the cell processing consumable pipeline group;

[0022] The first tubing assembly is installed on the first drive pump, which is located between the first connection point and the centrifuge container; the second tubing assembly is installed on the second drive pump, which is located between the second connection point and the centrifuge container; the third drive pump is installed on the second sorting pipeline; the first tubing assembly is installed on the first switch valve, which and the centrifuge container are located on opposite sides of the first drive pump; the second tubing assembly is installed on the second switch valve, which and the centrifuge container are located on opposite sides of the second drive pump; the first connection pipeline is installed on the third switch valve; the first sorting pipeline is installed on the fourth switch valve; the second sorting pipeline is installed on the fifth switch valve, which is located between the third drive pump and the third connection point.

[0023] The cell processing consumable tubing assembly provided by this utility model includes a centrifuge container, a first tubing assembly connected to the centrifuge container and used for matching a first drive pump, a second tubing assembly connected to the centrifuge container and used for matching a second drive pump, a first connecting pipe, a magnetic sorting column for sorting cells labeled with magnetic beads by magnetic adsorption, a first sorting pipe, and a second sorting pipe for matching a third drive pump; one end of the first connecting pipe is connected to the first tubing assembly and located at a first connecting point, and the other end of the first connecting pipe is connected to the second tubing assembly and located at a second connecting point; the first connecting point and the centrifuge container are respectively located on opposite sides of the first drive pump, and the second connecting point and the centrifuge container are respectively located on opposite sides of the second drive pump; the first sorting pipe, the magnetic sorting column, and the second sorting pipe are connected in sequence, the end of the first sorting pipe away from the magnetic sorting column is connected to the first tubing assembly and located at a third connecting point, and the end of the second sorting pipe away from the magnetic sorting column is connected to the first tubing assembly and located at a fourth connecting point, the third connecting point and the fourth connecting point are respectively located on opposite sides of the first drive pump.

[0024] The cell processing consumable tubing assembly provided by this invention connects a first tubing assembly and a second tubing assembly to a centrifuge container, with a connecting pipe between the two sets. This allows for selective input of solutions connected to the first tubing assembly into the centrifuge container via the second tubing assembly, solutions connected to the second tubing assembly into the centrifuge container via the first tubing assembly, circulation of the solution within the consumable tubing assembly, or input of liquid from different positions within the centrifuge container into different containers connected to either the first or second tubing assembly. Furthermore, a magnetic sorting column is connected to different positions within the second tubing assembly via first and second sorting pipes. The magnetic sorting column can generate or eliminate a magnetic field, enabling cell sorting within the column. This allows for cell sorting within the consumable tubing assembly without changing equipment. Thus, multiple processes can be completed sequentially within the consumable tubing assembly during cell processing, eliminating the need to change equipment after each process, avoiding cell contamination, and improving cell processing efficiency. Attached Figure Description

[0025] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0026] Figure 1 This is a schematic diagram of the structure of a cell processing consumable tube assembly provided in one embodiment of the present invention.

[0027] Figure 2 This is a schematic diagram of the structure of a cell processing device provided in one embodiment of the present invention.

[0028] The reference numerals in the accompanying drawings are as follows:

[0029] 111. Centrifuge container; 112. First tubing assembly; 113. First centrifuge line; 114. Second process line; 115. First transfer line; 116. Second tubing assembly; 117. Second centrifuge line; 118. Third process line; 119. Sample line; 120. Third waste line; 121. Harvest line; 122. Second transfer line; 123. First connecting line; 124. Second connecting line; 125. Third connecting line; 126. Magnetic separation column; 127. First sorting line; 128. Second sorting line; 129. Fluid filter; 130. Separation column; 131. First process line; 132. First waste line Piping; 133, Fourth connecting pipe; 134, Fifth connecting pipe; 135, Sixth connecting pipe; 136, Second waste liquid pipe; 210, First process solution container; 220, First waste liquid container; 230, Second waste liquid container; 240, First transfer liquid container; 250, Third waste liquid container; 260, Second transfer liquid container; 310, First drive pump; 320, Second drive pump; 330, Third drive pump; 410, First switching valve; 420, Second switching valve; 430, Third switching valve; 440, Fourth switching valve; 450, Fifth switching valve; 500, Pipe clamp; 700, Bubble sensor; 800, Pressure sensor. Detailed Implementation

[0030] To make the technical problems solved, technical solutions, and beneficial effects of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain this utility model and are not intended to limit this utility model.

[0031] like Figure 1As shown, one embodiment of this utility model provides a cell processing consumable tubing assembly, including a centrifuge container 111, a first tubing assembly 112 connected to the centrifuge container 111 and used for matching a first drive pump 310, a second tubing assembly 116 connected to the centrifuge container 111 and used for matching a second drive pump 320, a first connecting pipe 123, a magnetic sorting column 126 for sorting cells labeled with magnetic beads by magnetic adsorption, a first sorting pipe 127, and a second sorting pipe 128 for matching a third drive pump 330; one end of the first connecting pipe 123 is connected to the first tubing assembly 112 and located at a first connection point, and the other end of the first connecting pipe 123 is connected to the second tubing assembly 116 and located at a second connection point. The first connection point and the centrifuge container 111 are located on opposite sides of the first drive pump 310, and the second connection point and the centrifuge container 111 are located on opposite sides of the second drive pump 320, respectively; the first sorting pipe 127, the magnetic sorting column 126 and the second sorting pipe 128 are connected in sequence, the end of the first sorting pipe 127 away from the magnetic sorting column 126 is connected to the first pipe group 112 and is located at the third connection point, the end of the second sorting pipe 128 away from the magnetic sorting column 126 is connected to the first pipe group 112 and is located at the fourth connection point, and the third connection point and the fourth connection point are located on opposite sides of the first drive pump 310, respectively.

[0032] Understandably, the magnetic sorting column 126 can generate or eliminate a magnetic field under the action of an external magnetic generator. For example, a magnetic generator can be fixedly installed near the magnetic sorting column 126, generating or eliminating a magnetic field within the column (e.g., an electromagnet). Alternatively, a magnetic generator (e.g., a magnet) can be brought near or moved away from the column, causing a magnetic field to be generated within the column when the generator approaches and eliminated when it moves away. After a magnetic field is generated within the column 126, it can magnetically sort the flowing liquid (i.e., magnetically labeled cells are magnetically adsorbed into the column under the magnetic field of the external magnetic generator). The cell treatment consumable tubing can be equipped with... Figure 2The components shown include switching valves for controlling the on / off state of the pipeline (e.g., first switching valve 410, second switching valve 420, third switching valve 430, fourth switching valve 440, and fifth switching valve 450), pipe clamps 500 for controlling the on / off state of the pipeline, bubble sensors 700 for detecting the fluid flow state in the pipeline, and pressure sensors 800 for detecting the fluid pressure in the pipeline, thereby achieving automated cell processing. The parameters of the above-mentioned switching valves can be set according to actual conditions; they can be the same type of switching valve or different types of switching valves. Centrifuge operations or culture operations can be performed in the centrifuge container 111. The ends of the first tube group 112 and the second tube group 116 furthest from the centrifuge container 111 can be connected to various process solution containers (including but not limited to one or more of the following: a washing solution container for holding washing solution, an antibody container for holding antibodies, a culture medium container for holding culture medium, a separation solution container for holding separation solution, a cryopreservation solution container for holding cryopreservation solution, or a virus sample container for holding virus samples), a transfer solution container for holding excess PBMC solution during centrifugation of the centrifuge container 111, a sample container for holding sample solution, a waste liquid container for holding waste liquid, or a harvest container for holding cell solution that needs to be sealed and harvested after cell processing. The first tube group 112 can be a single tube group with the same diameter; the first tube group 112 can also be a single tube group or a segmented tube group with different diameters, so that the diameter of the part where the first drive pump 310 is set is set according to the parameters of the first drive pump 310 to match the first drive pump 310. The second tube assembly 116 can be a single, integral tube assembly with the same diameter; alternatively, it can be a single or segmented tube assembly with different diameters, allowing the diameter of the section containing the second drive pump 320 to be set according to its parameters to match the second drive pump 320. The connection methods between the centrifuge container 111, the first tube assembly 112, the second tube assembly 116, the first connecting pipe 123, the magnetic sorting column 126, the first sorting pipe 127, and the second sorting pipe 128 include, but are not limited to, one or more methods such as integral molding or multi-port connectors. The ends of the first tube assembly 112 and the second tube assembly 116 connecting to the centrifuge container 111 can be located in different positions within the centrifuge container 111, such as at the top and bottom, or in the middle and on the side, respectively.

[0033] In this embodiment, the connecting pipe connects the first tube group 112 and the second tube group 116, allowing the solution connected to the first tube group 112 to be input into the centrifuge container 111 through the second tube group 116, and vice versa. A circulation pipeline can be formed as needed to allow the liquid to circulate within the cell processing consumable tube group. Furthermore, the liquid in the centrifuge container 111 can be input from different positions within the centrifuge container 111 (e.g., above or below) into different containers connected to either the first tube group 112 or the second tube group 116, as needed. The magnetic sorting column 126 is connected to different positions in the second tube group 116 via the first sorting pipe 127 and the second sorting pipe 128, enabling cell sorting within the cell processing consumable tube group without changing the equipment.

[0034] The cell processing consumable tubing assembly provided by this utility model connects a first tubing assembly 112 and a second tubing assembly 116 to a centrifuge container 111, and a connecting pipe is provided between the first tubing assembly 112 and the second tubing assembly 116. This allows for selective input of solutions connected to the first tubing assembly 112 into the centrifuge container 111 via the second tubing assembly 116, solutions connected to the second tubing assembly 116 into the centrifuge container 111 via the first tubing assembly 112, enabling the solution to circulate within the cell processing consumable tubing assembly, or allowing liquid in the centrifuge container 111 to be input from different positions within the centrifuge container 111 into different containers connected to the first tubing assembly 112 or the second tubing assembly 116. Furthermore, a magnetic sorting column 126 is connected to different positions in the second tubing assembly 116 via a first sorting pipe 127 and a second sorting pipe 128. The magnetic sorting column 126 can generate or eliminate a magnetic field, thereby enabling cell sorting within the magnetic sorting column 126. Thus, cell sorting is achieved within the cell processing consumable tubing assembly without changing the equipment. In this way, multiple processes can be completed sequentially within the cell processing consumables tubing during cell processing, eliminating the need to change equipment after each process to proceed to the next, thus avoiding cell contamination and improving cell processing efficiency.

[0035] like Figure 2As shown, in one embodiment, the cell processing consumable tubing assembly further includes a second connecting conduit 124; one end of the second connecting conduit 124 is connected to the second tubing assembly 116 and located at a fifth connecting point, and the other end of the second connecting conduit 124 is connected to the second tubing assembly 116 and located at a sixth connecting point; the fifth connecting point and the sixth connecting point are respectively located on opposite sides of the second drive pump 320. In one embodiment, the cell processing consumable tubing assembly further includes a third connecting conduit 125; one end of the third connecting conduit 125 is connected to the first tubing assembly 112 and located at a seventh connecting point, and the other end of the second connecting conduit 124 is connected to the second tubing assembly 116 and located at an eighth connecting point; the seventh connecting point is located between the first drive pump 310 and the centrifuge container 111, and the eighth connecting point is located between the second drive pump 320 and the centrifuge container 111. It can be understood that the first connecting point and the third connecting point can be the same connecting point or different connecting points. The second connecting point and the fifth connecting point can be the same connecting point or different connecting points. The fourth connection point and the seventh connection point can be the same connection point or different connection points. The connection method between the second connecting pipe 124 and the second pipe group 116 includes, but is not limited to, one or more of the following: integral molding connection or multi-way connector connection. The connection method between the third connecting pipe 125 and the first pipe group 112 and the second pipe group 116 includes, but is not limited to, one or more of the following of the following: integral molding connection or multi-way connector connection. The second connecting pipe 124 and the third connecting pipe 125 can be equipped with... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, thereby allowing the liquid flow path to be selected according to the actual situation.

[0036] like Figure 2As shown, in one embodiment, the cell processing consumable tubing assembly further includes a first process tubing 131 for connecting to the first process solution container 210; one end of the first process tubing 131, away from the first process solution container 210, is connected to the second sorting tubing 128 and located at a ninth connection point; the ninth connection point is located between the third drive pump 330 and the fourth connection point. It is understood that the ninth connection point and the fourth connection point can be the same connection point or different connection points. The connection method between the second sorting tubing 128 and the first process tubing 131 includes, but is not limited to, one or more of the following: integral molding connection or multi-port connector connection. There can be one or more first process tubing lines 131, thereby connecting different containers. In one embodiment, the first process solution container 210 includes, but is not limited to, a magnetic bead container or a culture medium container; the first process tubing 131 includes, but is not limited to, a magnetic bead tubing for connecting to the magnetic bead container or a culture medium tubing for connecting to the culture medium container. The first process tubing 131 may be provided with, for example... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, allowing the selection of the required process solution based on actual conditions.

[0037] like Figure 2 As shown, in one embodiment, the first sorting pipeline 127 is provided with a fluid filter 129 for filtering large particulate impurities and a separation column 130 for adsorbing some cells; the fluid filter 129 is located between the third connection point and the separation column 130. It is understood that the impurities filtered by the fluid filter 129 include blood clots, microaggregates, and particulate debris in blood, as well as blood clots and particulate fragments in salvaged blood products. The partial cells adsorbed by the separation column 130 refer to components in the liquid that need to be captured and removed by the separation column 130 and do not need to appear in the final product, including but not limited to viscous cells such as monocytes, granulocytes, and platelets present in the cell suspension during blood cell separation, as well as components that may be non-specifically bound to the magnetic sorting column 126 and therefore need to be pre-filtered by the separation column 130.

[0038] In this embodiment, the first sorting pipeline 127 is simultaneously equipped with the fluid filter 129, the separation column 130, and the magnetic separation column 126, thereby enabling three-stage sorting of liquids (such as sample solutions). Specifically, firstly, impurities in the liquid are filtered out in the fluid filter 129, and the remaining liquid is output to the separation column 130. Then, the separation column 130 separates some of the cells from the liquid, and the remaining liquid is output to the magnetic separation column 126. Then, the magnetic separation column 126 magnetically adsorbs the cells marked with magnetic beads. In this way, the three-stage sorting avoids the blockage of the magnetic separation column 126 by impurities and viscous cells in the liquid. Thus, the cells marked with magnetic beads can be accurately sorted, improving sorting efficiency and accuracy.

[0039] like Figure 2 As shown, in one embodiment, the cell processing consumable tubing assembly further includes a first waste liquid pipeline 132 for connecting to a first waste liquid container 220; one end of the waste liquid pipeline away from the waste liquid container is connected to the first sorting pipeline 127 and located at the tenth connection point; the tenth connection point is located between the separation column 130 and the magnetic sorting column 126. It is understood that the connection method between the first sorting pipeline 127 and the first waste liquid pipeline 132 includes, but is not limited to, one or more of the following: integral molding connection or multi-port connector connection. The first waste liquid pipeline 132 may be provided with... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, so that waste liquid can be output according to the actual situation.

[0040] like Figure 2 As shown, in one embodiment, the cell processing consumable tubing assembly further includes a fourth connecting conduit 133; one end of the fourth connecting conduit 133 is connected to the first sorting conduit 127 and located at the eleventh connecting point, and the other end of the fourth connecting conduit 133 is connected to the second sorting conduit 128 and located at the twelfth connecting point; the eleventh connecting point is located between the magnetic sorting column 126 and the tenth connecting point, and the twelfth connecting point is located between the third drive pump 330 and the fourth connecting point. It is understood that the twelfth connecting point and the ninth connecting point can be the same connecting point or different connecting points. The eleventh connecting point and the tenth connecting point can be the same connecting point or different connecting points. The connection method between the fourth connecting conduit 133 and the first sorting conduit 127 and the second sorting conduit 128 includes, but is not limited to, one or more of the following: integral molding connection or multi-port connector connection. The fourth connecting conduit 133 may be provided with, for example... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, thereby allowing the fluid flow path to be selected according to the actual situation.

[0041] like Figure 2 As shown, in one embodiment, the cell processing consumable tubing assembly further includes a fifth connecting conduit 134; one end of the fifth connecting conduit 134 is connected to the first sorting conduit 127 and located at the thirteenth connecting point, and the other end of the fifth connecting conduit 134 is connected to the first waste liquid conduit 132; the thirteenth connecting point is located between the third connecting point and the fluid filter 129. It is understood that the connection method between the fifth connecting conduit 134 and the first sorting conduit 127 and the first waste liquid conduit 132 includes, but is not limited to, one or more of the following: integral molding connection or multi-port connector connection. The fifth connecting conduit 134 may be provided with... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, thereby allowing the fluid flow path to be selected according to the actual situation.

[0042] like Figure 2 As shown, in one embodiment, the cell treatment consumable tubing assembly further includes a sixth connecting conduit 135; one end of the sixth connecting conduit 135 is connected to the first waste liquid conduit 132, and the other end of the sixth connecting conduit 135 is connected to the first process conduit 131. It is understood that the connection point between the fifth connecting conduit 134 and the first waste liquid conduit 132, and the connection point between the sixth connecting conduit 135 and the first waste liquid conduit 132, can be the same connection point or different connection points. The connection method between the sixth connecting conduit 135 and the first process conduit 131 and the first waste liquid conduit 132 includes, but is not limited to, one or more of the following: integral molding connection or multi-port connector connection. The sixth connecting conduit 135 may be provided with... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, thereby allowing the fluid flow path to be selected according to the actual situation.

[0043] like Figure 2 As shown, in one embodiment, the cell processing consumable tubing assembly further includes a second waste liquid pipeline 136 for connecting to a second waste liquid container 230; one end of the second waste liquid pipeline, away from the second waste liquid container, is connected to the second sorting pipeline 128 and located at the fourteenth connection point; the fourteenth connection point is located between the third drive pump 330 and the fourth connection point. It is understood that the connection method between the second sorting pipeline 128 and the second waste liquid pipeline 136 includes, but is not limited to, one or more of the following: integral molding connection or multi-port connector connection. The second waste liquid pipeline 136 may be provided with... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, so that waste liquid can be output according to the actual situation.

[0044] like Figure 2As shown, in one embodiment, the first tubing assembly 112 includes a first centrifugal line 113 for matching the first drive pump 310, a second process line 114 for connecting to a second process solution container, and a first transfer line 115 for connecting to a first transfer liquid container 240. One end of the first centrifugal line 113 is connected to the centrifugal container 111, and the other end of the first centrifugal line 113 is connected to the end of the second process line 114 away from the second process solution container. The end of the first transfer line 115 away from the first transfer liquid container 240 is connected to the second process line 114. It is understood that the connection point between the first centrifugal line 113 and the second process line 114 can be the same connection point as the first connection point, or it can be a different connection point. The connection method between the second process line 114 and the first transfer line 115 and the first centrifugal line 113 includes, but is not limited to, one or more of the following: integral molding connection or multi-port connector connection. There can be one or more second process lines 114, thereby connecting different containers. In one embodiment, the second process solution container includes, but is not limited to, a cleaning solution container or an antibody container; the second process pipeline 114 includes, but is not limited to, a cleaning pipeline for connecting to the cleaning solution container or an antibody pipeline for connecting to the antibody container. The first centrifuge pipeline 113 and the second process pipeline 114 may be equipped with, for example... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, allowing selection of the required process solution based on actual conditions. There can be one or more first transfer pipelines 115, allowing connection to one or more first transfer liquid containers 240 as needed. For example... Figure 2 As shown, the first transfer pipeline 115 can share a switch valve with the second process pipeline 114. At the same time, pipe clamps 500 are respectively installed on the first transfer pipeline 115 and the second process pipeline 114, so that the flow path of the liquid can be selected according to the actual situation.

[0045] like Figure 2As shown, in one embodiment, the second tubing assembly 116 includes a second centrifugal line 117 for matching the second drive pump 320, and a third process line 118 for connecting to a third process solution container; one end of the second centrifugal line 117 is connected to the centrifugal container 111, and the other end of the second centrifugal line 117 is connected to the end of the third process line 118 away from the third process solution container. It is understood that the connection point between the second centrifugal line 117 and the third process line 118 can be the same as the second connection point, or it can be a different connection point. The connection method between the second centrifugal line 117 and the third process line 118 includes, but is not limited to, one or more of the following: integral molding connection or multi-port connection. There can be one or more third process lines 118, thereby connecting different containers. In one embodiment, the third process solution container includes, but is not limited to, a culture medium container, a separation solution container, a cryopreservation solution container, or a virus sample container; the third process tubing 118 includes, but is not limited to, a culture medium tubing for connecting to the culture medium container, a separation solution tubing for connecting to the separation solution container, a cryopreservation solution tubing for connecting to the cryopreservation solution container, or a virus sample tubing 119 for connecting to the virus sample container. The second centrifuge tubing 117 and the third process tubing 118 may be equipped with, for example... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, allowing the selection of the required process solution based on actual conditions.

[0046] like Figure 2 As shown, in one embodiment, the second tube assembly 116 further includes a sample tube 119 for connecting to a sample container; the end of the second centrifuge tube 117 away from the centrifuge container 111 is connected to the end of the sample tube 119 away from the sample container. It is understood that the connection point between the second centrifuge tube 117 and the sample tube 119 can be the same connection point or a different connection point. The connection method between the second centrifuge tube 117 and the sample tube 119 includes, but is not limited to, one or more of the following: integral molding connection or multi-port connector connection. The sample tube 119 may be provided with... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, allowing selection of whether sample solution needs to be introduced based on actual conditions.

[0047] like Figure 2As shown, in one embodiment, the second pipe assembly 116 further includes a third waste liquid pipeline 120 for connecting to the third waste liquid container 250; the end of the second centrifugal pipeline 117 away from the centrifugal container 111 is connected to the end of the third waste liquid pipeline 120 away from the third waste liquid container 250. It is understood that the connection point between the second centrifugal pipeline 117 and the third waste liquid pipeline 120 can be the same connection point as the second connection point, or it can be a different connection point. The connection method between the second centrifugal pipeline 117 and the third waste liquid pipeline 120 includes, but is not limited to, one or more of the following: integral molding connection or multi-port connector connection. The third waste liquid pipeline 120 may be provided with... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, so that waste liquid can be output according to the actual situation.

[0048] like Figure 2 As shown, in one embodiment, the second pipe assembly 116 further includes a harvesting pipe 121 for connecting to a harvesting container, and the end of the second centrifugal pipe 117 away from the centrifugal container 111 is connected to the end of the harvesting pipe 121 away from the harvesting container. It is understood that the connection point between the second centrifugal pipe 117 and the harvesting pipe 121 can be the same connection point or a different connection point. The connection method between the second centrifugal pipe 117 and the harvesting pipe 121 includes, but is not limited to, one or more of the following: integral molding connection or multi-port connector connection. The harvesting pipe 121 may be provided with... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, allowing selection of whether a final product needs to be output based on actual conditions.

[0049] like Figure 2 As shown, in one embodiment, the second tubing assembly 116 further includes a second transfer line 122 for connecting to the second transfer liquid container 260; one end of the second transfer line 122, away from the second transfer liquid container 260, is connected to the third process line 118. It is understood that the connection between the second transfer line 122 and the third process line 118 can be, but is not limited to, one or more of the following: integral connection or connection via a multi-port connector. There can be one or more second transfer lines 122, thereby allowing connection to one or more second transfer liquid containers 260 as needed. Figure 2 As shown, the second transfer pipeline 122 can share a switch valve with the third process pipeline 118. At the same time, pipe clamps 500 are respectively installed on the second transfer pipeline 122 and the third process pipeline 118, so that the flow path of the liquid can be selected according to the actual situation.

[0050] like Figure 2As shown, one embodiment of this utility model also provides a cell processing device, including a first drive pump 310, a second drive pump 320, a third drive pump 330, at least one first switching valve 410 for controlling the on / off of the pipeline in the first pipe group 112, at least one second switching valve 420 for controlling the on / off of the pipeline in the second pipe group 116, a third switching valve 430 for controlling the on / off of the first connecting pipeline 123, a fourth switching valve 440 for controlling the on / off of the first sorting pipeline 127, a fifth switching valve 450 for controlling the on / off of the second sorting pipeline 128, and the cell processing consumable pipe group. The first pipe assembly 112 is installed on the first drive pump 310, which is located between the first connection point and the centrifuge container 111. The second pipe assembly 116 is installed on the second drive pump 320, which is located between the second connection point and the centrifuge container 111. The third drive pump 330 is installed on the second sorting pipe 128. The first pipe assembly 112 is installed on the first switch valve 410, which is located on opposite sides of the first drive pump 310. The second pipe assembly 116 is installed on the second switch valve 420, which is located on opposite sides of the second drive pump 320. The first connecting pipe 123 is installed on the third switch valve 430. The first sorting pipe 127 is installed on the fourth switch valve 440. The second sorting pipe 128 is installed on the fifth switch valve 450, which is located between the third drive pump 330 and the third connection point. Understandably, the first drive pump 310 is used to drive the liquid flow in the first tubing assembly 112. The first drive pump 310 includes, but is not limited to, a peristaltic pump, which can drive the liquid flow without contacting the liquid, thus preventing contamination. The second drive pump 320 is used to drive the liquid flow in the second tubing assembly 116. The second drive pump 320 includes, but is not limited to, a peristaltic pump, which can drive the liquid flow without contacting the liquid, thus preventing contamination. The third drive pump 330 is used to drive the liquid flow in the second sorting pipeline 128. The second drive pump 320 includes, but is not limited to, a peristaltic pump, which can drive the liquid flow without contacting the liquid, thus preventing contamination. The first drive pump 310, the second drive pump 320, or the third drive pump 330 can also be other types of liquid pumps, as long as they can achieve the effect of driving liquid flow.

[0051] The cell processing device provided by this utility model connects a first tube group 112 and a second tube group 116 to a centrifuge container 111, and a connecting pipe is provided between the first tube group 112 and the second tube group 116. This allows for selective input of the solution connected to the first tube group 112 into the centrifuge container 111 via the second tube group 116, or the solution connected to the second tube group 116 into the centrifuge container 111 via the first tube group 112, enabling the solution to circulate within the cell processing consumable tube group. Alternatively, liquid in the centrifuge container 111 can be input from different positions within the centrifuge container 111 into different containers connected to the first tube group 112 or the second tube group 116. Furthermore, a magnetic sorting column 126 is connected to different positions in the second tube group 116 via a first sorting pipe 127 and a second sorting pipe 128. The magnetic sorting column 126 can generate or eliminate a magnetic field, thereby enabling cell sorting within the column. Thus, cell sorting is achieved within the cell processing consumable tube group without replacing the equipment. In this way, multiple processes can be completed sequentially within the cell processing consumables tubing during cell processing, eliminating the need to change equipment after each process to proceed to the next, thus avoiding cell contamination and improving cell processing efficiency.

[0052] The above are merely embodiments of the cell processing consumable tubing and cell processing equipment of this utility model, and are not intended to limit this utility model. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A cell processing consumable tube set, comprising: The cell processing consumable tube set comprises a centrifuge container, a first tube group connected with the centrifuge container and matched with a first driving pump, a second tube group connected with the centrifuge container and matched with a second driving pump, a first connecting pipeline, a magnetic separation column for separating cells labeled by magnetic beads through magnetic adsorption, a first separation pipeline, and a second separation pipeline matched with a third driving pump; One end of the first connecting pipeline is connected with the first tube group at a first connecting point, and the other end of the first connecting pipeline is connected with the second tube group at a second connecting point; the first connecting point and the centrifuge container are located on opposite sides of the first driving pump respectively, and the second connecting point and the centrifuge container are located on opposite sides of the second driving pump respectively; the first separation pipeline, the magnetic separation column and the second separation pipeline are connected in sequence, one end of the first separation pipeline away from the magnetic separation column is connected with the first tube group at a third connecting point, and one end of the second separation pipeline away from the magnetic separation column is connected with the first tube group at a fourth connecting point; the third connecting point and the fourth connecting point are located on opposite sides of the first driving pump respectively.

2. The cell processing consumables tube set of claim 1, wherein, The cell processing consumable tube set further comprises a second connecting pipeline; one end of the second connecting pipeline is connected with the second tube group at a fifth connecting point, and the other end of the second connecting pipeline is connected with the second tube group at a sixth connecting point; the fifth connecting point and the sixth connecting point are located on opposite sides of the second driving pump respectively; and / or The cell processing consumable tube set further comprises a third connecting pipeline; one end of the third connecting pipeline is connected with the first tube group at a seventh connecting point, and the other end of the second connecting pipeline is connected with the second tube group at an eighth connecting point; the seventh connecting point is located between the first driving pump and the centrifuge container, and the eighth connecting point is located between the second driving pump and the centrifuge container.

3. The cell processing consumables tube set of claim 1, wherein, The cell processing consumable tube set further comprises a first process pipeline for connecting a first process solution container; one end of the first process pipeline away from the first process solution container is connected with the second separation pipeline at a ninth connecting point; the ninth connecting point is located between the third driving pump and the fourth connecting point.

4. The cell processing consumables tube set of claim 3, wherein, A fluid filter for filtering large-particle impurities and a separation column for adsorbing part of cells are arranged on the first separation pipeline; the fluid filter is located between the third connecting point and the separation column.

5. The cell processing consumables tube set of claim 4, wherein, The cell processing consumable tube set further comprises a first waste liquid pipeline for connecting a first waste liquid container; one end of the first waste liquid pipeline away from the first waste liquid container is connected with the first separation pipeline at a tenth connecting point; the tenth connecting point is located between the separation column and the magnetic separation column.

6. The cell processing consumables tube set of claim 5, wherein, The cell processing consumable tube set further comprises a fourth communication tube; one end of the fourth communication tube is communicated on the first sorting tube and located at an eleventh communication point, the other end of the fourth communication tube is communicated on the second sorting tube and located at a twelfth communication point; the eleventh communication point is located between the magnetic sorting column and the tenth communication point, and the twelfth communication point is located between the third driving pump and the fourth communication point; and / or The cell processing consumable tube set further comprises a fifth communication tube; one end of the fifth communication tube is communicated on the first sorting tube and located at a thirteenth communication point, and the other end of the fifth communication tube is communicated on the first waste liquid tube; The thirteenth communication point is located between the third communication point and the fluid filter; And / or The cell processing consumable tube set further comprises a sixth communication tube; one end of the sixth communication tube is communicated on the first waste liquid tube, and the other end of the sixth communication tube is communicated on the first process tube.

7. The cell processing consumables tube set of claim 1, wherein, The cell processing consumable tube set further comprises a second waste liquid tube for communicating a second waste liquid container; one end of the second waste liquid tube away from the second waste liquid container is communicated on the second sorting tube and located at a fourteenth communication point; the fourteenth communication point is located between the third driving pump and the fourth communication point.

8. The cell processing consumables tube set of claim 1, wherein, The first tube set comprises a first centrifugal tube for matching the first driving pump, a second process tube for communicating a second process solution container, and a first transfer tube for communicating a first transfer liquid container; one end of the first centrifugal tube is communicated with the centrifugal container, and the other end of the first centrifugal tube is communicated with one end of the second process tube away from the second process solution container; one end of the first transfer tube away from the first transfer liquid container is communicated on the second process tube.

9. The cell processing consumables tube set of claim 1, wherein, The second tube set comprises a second centrifugal tube for matching the second driving pump, and a third process tube for communicating a third process solution container; one end of the second centrifugal tube is communicated with the centrifugal container, and the other end of the second centrifugal tube is communicated with one end of the third process tube away from the third process solution container; The second tube set further comprises a sample tube for communicating a sample container; one end of the second centrifugal tube away from the centrifugal container is communicated with one end of the sample tube away from the sample container; and / or The second tube set further comprises a third waste liquid tube for communicating a third waste liquid container; one end of the second centrifugal tube away from the centrifugal container is communicated with one end of the third waste liquid tube away from the third waste liquid container; and / or The second tube set further comprises a harvest tube for communicating a harvest container; one end of the second centrifugal tube away from the centrifugal container is communicated with one end of the harvest tube away from the harvest container; and / or The second tube set further comprises a second transfer tube for communicating a second transfer liquid container; one end of the second transfer tube away from the second transfer liquid container is communicated on the third process tube.

10. A cell processing device, characterized by The cell processing consumable tube set as claimed in any one of claims 1 to 9, comprising a first drive pump, a second drive pump, a third drive pump, at least one first on-off valve for controlling the on-off of a tube line in the first tube set, at least one second on-off valve for controlling the on-off of a tube line in the second tube set, a third on-off valve for controlling the on-off of the first communication line, a fourth on-off valve for controlling the on-off of the first sorting line, a fifth on-off valve for controlling the on-off of the second sorting line, and the first tube set is installed on the first drive pump, the first drive pump is located between the first communication point and the centrifugal container, the second tube set is installed on the second drive pump, the second drive pump is located between the second communication point and the centrifugal container, the third drive pump is installed on the second sorting line, the first tube set is installed on the first on-off valve, the first on-off valve and the centrifugal container are located on opposite sides of the first drive pump respectively, the second tube set is installed on the second on-off valve, the second on-off valve and the centrifugal container are located on opposite sides of the second drive pump respectively, the first communication line is installed on the third on-off valve, the first sorting line is installed on the fourth on-off valve, the second sorting line is installed on the fifth on-off valve, and the fifth on-off valve is located between the third drive pump and the third communication point. The cell processing consumable tube set as claimed in any one of claims 1 to 9, comprising a first drive pump, a second drive pump, a third drive pump, at least one first on-off valve for controlling the on-off of a tube line in the first tube set, at least one second on-off valve for controlling the on-off of a tube line in the second tube set, a third on-off valve for controlling the on-off of the first communication line, a fourth on-off valve for controlling the on-off of the first sorting line, a fifth on-off valve for controlling the on-off of the second sorting line, and the first tube set is installed on the first drive pump, the first drive pump is located between the first communication point and the centrifugal container, the second tube set is installed on the second drive pump, the second drive pump is located between the second communication point and the centrifugal container, the third drive pump is installed on the second sorting line, the first tube set is installed on the first on-off valve, the first on-off valve and the centrifugal container are located on opposite sides of the first drive pump respectively, the second tube set is installed on the second on-off valve, the second on-off valve and the centrifugal container are located on opposite sides of the second drive pump respectively, the first communication line is installed on the third on-off valve, the first sorting line is installed on the fourth on-off valve, the second sorting line is installed on the fifth on-off valve, and the fifth on-off valve is located between the third drive pump and the third communication point. The cell processing consumable tube set as claimed in any one of claims 1 to 9, comprising a first drive pump, a second drive pump, a third drive pump, at least one first on-off valve for controlling the on-off of a tube line in the first tube set, at least one second on-off valve for controlling the on-off of a tube line in the second tube set, a third on-off valve for controlling the on-off of the first communication line, a fourth on-off valve for controlling the on-off of the first sorting line, a fifth on-off valve for controlling the on-off of the second sorting line, and the first tube set is installed on the first drive pump, the first drive pump is located between the first communication point ​

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