A culture device for endometrial cancer organoids

Abstract

The utility model discloses a kind of culture devices for endometrial carcinoma organoids, including culture mechanism and speed control mechanism, the culture mechanism includes culture base, the infusion tube of top portion of culture base is arranged in the inclination, the pipe head being connected with the bottom end of the infusion tube, the graduated cylinder being sleeved in the top end of the infusion tube, speed control mechanism, the speed control mechanism includes two stop blocks one and multiple stop blocks two being connected with the bottom of the infusion tube inner cavity.The utility model in, liquid in graduated cylinder flows through infusion tube, then from pipe head place enters into culture base, in this process, culture solution passes through stop block one and multiple stop blocks two, when passing through stop block one, culture solution is shunted into two, two culture solution respectively collides two stop blocks two, stop block two buffer culture solution flow rate, and make it again branch, effectively avoid culture solution splash phenomenon.

Description

Technical Field

[0001] This utility model relates to the field of culture medium technology, specifically to a culture device for endometrial cancer organoids. Background Technology

[0002] Endometrial cancer is a highly heterogeneous malignant tumor, and its etiology is not yet fully understood. With the development of organoid technology, constructing in vitro models of tumor organoids derived from patients can accurately simulate the biological characteristics of in vivo tumors and their response to drug therapy, which is of great value for tumor research, drug testing, and new drug screening. Traditional tumor cell line models have low success rates, and two-dimensional culture methods result in tumor cells losing their three-dimensional structure and lacking heterogeneity, thus failing to accurately replicate the biological characteristics of in vivo tumors.

[0003] Application number CN202321055005.5 discloses a tumor organoid culture medium, including a rotating disc and other structures. A capping assembly removes the cover of the culture medium, and then a second drive motor is activated to rotate the rotating shaft counterclockwise, moving the infusion gun directly above the culture medium. The infusion gun then delivers the culture medium from the reservoir into the culture medium, controlling the amount of medium input to achieve the required quantity. This avoids the situation where insufficient culture medium input due to manual infusion, which can alter the development time of tumor organoids in the culture medium and lead to errors in experimental data. However, in practical use, the flow rate of the culture medium is not controlled, and the infusion gun is far from the culture medium, causing the liquid to easily splash when flowing down. Utility Model Content

[0004] This utility model aims to solve one of the technical problems existing in the prior art or related technologies.

[0005] Therefore, the technical solution adopted by this utility model is as follows:

[0006] A culture device for organoids of endometrial cancer includes a culture mechanism and a speed control mechanism. The culture mechanism includes a culture tray, an infusion tube inclinedly disposed on top of the culture tray, a tube head connected to the bottom end of the infusion tube, and a measuring cylinder sleeved on the top end of the infusion tube. The speed control mechanism includes two first blocks and multiple second blocks connected to the bottom of the inner lumen of the infusion tube. The multiple second blocks are arranged in pairs, forming two groups. The two second blocks in each group are vertically symmetrical about the first blocks, and the two first blocks and the two groups of second blocks are staggered.

[0007] By adopting the above technical solution, the liquid in the measuring cylinder flows through the infusion tube and then enters the culture medium tray from the tube head. During this process, the culture medium passes through baffle one and multiple baffle two. When passing through baffle one, the culture medium is split into two streams. The two streams of culture medium collide with two baffle two respectively. Baffle two buffers the flow rate of the culture medium and splits it into streams again, effectively avoiding the phenomenon of culture medium splashing.

[0008] In a preferred embodiment, the present invention can be further configured such that: a measuring mechanism is provided on the outside of the infusion tube, the measuring mechanism including a micro pump connected and communicating with a measuring cylinder, a thin tube connected to the extraction end of the micro pump, a storage cylinder sleeved at the end of the thin tube, and a solenoid valve installed on the infusion tube.

[0009] In a preferred embodiment, the present invention can be further configured such that a mounting base is connected between the measuring cylinder and the micro pump, and the mounting base has an opening located on the front side of the measuring cylinder.

[0010] In a preferred embodiment, the present invention can be further configured such that: a horizontal platform is installed at the bottom of the mounting base, and the bottom of the horizontal platform is fixedly connected to the top of the liquid storage cylinder.

[0011] In a preferred embodiment, the present invention can be further configured such that: a plurality of reinforcing ribs are connected between the liquid storage cylinder and the cross platform, and the plurality of reinforcing ribs are equally spaced and arranged in a ring.

[0012] By adopting the above technical solution, the beneficial effects achieved by this utility model are as follows:

[0013] 1. In this utility model, the liquid in the measuring cylinder flows through the infusion tube and then enters the culture medium tray from the tube head. During this process, the culture medium passes through baffle one and multiple baffle two. When passing through baffle one, the culture medium is split into two streams. The two streams of culture medium collide with two baffle two respectively. Baffle two buffers the flow rate of the culture medium and splits it into streams again, effectively avoiding the phenomenon of culture medium splashing.

[0014] 2. In this invention, the micro pump draws the culture medium from the storage cylinder through a thin tube and injects it into the measuring cylinder. Then, with the solenoid valve closed, the measuring cylinder accurately measures the amount of culture medium, providing an appropriate amount of culture medium to the endometrial cancer organoids and preventing waste of culture medium. Attached Figure Description

[0015] Figure 1 This is a perspective view of the overall structure of this utility model;

[0016] Figure 2 This is a schematic diagram of the cultivation mechanism of this utility model;

[0017] Figure 3 This is a schematic diagram of the speed control mechanism of this utility model;

[0018] Figure 4 This is a schematic diagram of the measuring mechanism of this utility model.

[0019] Figure label:

[0020] 100. Culture apparatus; 110. Culture medium tray; 120. Infusion tubing; 130. Tube tip; 140. Measuring cylinder;

[0021] 200. Speed ​​control mechanism; 210. Stop block one; 220. Stop block two;

[0022] 300. Measuring mechanism; 310. Miniature pump; 320. Thin tube; 330. Liquid reservoir; 340. Solenoid valve;

[0023] 400. Mounting bracket;

[0024] 500, horizontal platform;

[0025] 600. Reinforcing ribs. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to specific embodiments and accompanying drawings. It should be noted that, unless otherwise specified, the embodiments and features of the present utility model can be combined with each other.

[0027] It should be understood that these descriptions are merely exemplary and not intended to limit the scope of this invention.

[0028] The following describes, with reference to the accompanying drawings, some embodiments of the present invention, providing a culture device for endometrial cancer organoids.

[0029] Example 1:

[0030] Combination Figure 1-4 As shown, the present invention provides a culture device for endometrial cancer organoids, including a culture mechanism 100 and a speed control mechanism 200. The culture mechanism 100 includes a culture medium tray 110, an infusion tube 120 inclinedly disposed on the top of the culture medium tray 110, a tube head 130 connected to the bottom end of the infusion tube 120, and a measuring cylinder 140 sleeved on the top end of the infusion tube 120.

[0031] The speed control mechanism 200 includes two first blocks 210 and multiple second blocks 220 connected to the bottom of the inner cavity of the infusion tube 120.

[0032] Multiple baffles 220 are arranged in pairs, forming two groups. In each group, the two baffles 220 are vertically symmetrical about the baffle 1 210. The two baffles 1 210 and the two groups of baffles 220 are staggered. The layout design of the baffles 1 210 and baffles 220 can effectively slow down the flow of culture medium through the infusion tube 120.

[0033] Example 2:

[0034] Combination Figure 1 and Figure 4 As shown, based on Embodiment 1, a measuring mechanism 300 is provided on the outside of the infusion tube 120. The measuring mechanism 300 includes a micro pump 310 connected and communicating with the measuring cylinder 140, a thin tube 320 connected to the extraction end of the micro pump 310, a reservoir 330 sleeved at the end of the thin tube 320, and a solenoid valve 340 installed on the infusion tube 120. After the micro pump 310 draws the culture medium in the reservoir 330 through the thin tube 320, it will be injected into the measuring cylinder 140. Then, with the solenoid valve 340 closed, the measuring cylinder 140 accurately measures the amount of culture medium and provides an appropriate amount of nutrient solution to the endometrial cancer organoid.

[0035] Example 3:

[0036] Combination Figure 1 and Figure 4 As shown, in the above embodiment, a mounting base 400 is connected between the measuring cylinder 140 and the micro pump 310. The mounting base 400 has an opening located on the front side of the measuring cylinder 140. The mounting base 400 can reinforce the measuring cylinder 140 and the micro pump 310, and the opening makes it convenient for staff to check the scale on the measuring cylinder 140.

[0037] Furthermore, a crossbeam 500 is installed at the bottom of the mounting base 400. The bottom of the crossbeam 500 is fixedly connected to the top of the liquid storage cylinder 330. The crossbeam 500 can limit the culture medium tray 110 and reinforce the mounting base 400 and the components at the top of the mounting base 400.

[0038] Furthermore, a plurality of reinforcing ribs 600 are connected between the liquid storage cylinder 330 and the horizontal platform 500. The plurality of reinforcing ribs 600 are equally spaced and arranged in a ring. The setting of reinforcing ribs 600 can improve the connection between the liquid storage cylinder 330 and the horizontal platform 500.

[0039] The working principle and usage process of this utility model are as follows: When this device is put into actual use, the measuring cylinder 140 contains a suitable amount of culture medium. Then, the solenoid valve 340 is opened, and the liquid in the measuring cylinder 140 flows through the infusion tube 120 and then enters the culture medium tray 110 from the tube head 130. During this process, the culture medium passes through the first baffle 210 and multiple second baffles 220. When passing through the first baffle 210, the culture medium is divided into two streams. The two streams of culture medium collide with the two second baffles 220 respectively. The second baffles 220 buffer the flow rate of the culture medium and divide it into streams again, effectively avoiding the phenomenon of culture medium splashing.

[0040] Although embodiments of the present invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A culture device for organoids from endometrial cancer, characterized in that, include: The culture mechanism (100) includes a culture medium tray (110), an infusion tube (120) inclinedly disposed on the top of the culture medium tray (110), a tube head (130) connected to the bottom end of the infusion tube (120), and a measuring cylinder (140) sleeved on the top end of the infusion tube (120). Speed ​​control mechanism (200), the speed control mechanism (200) includes two first blocks (210) and multiple second blocks (220) connected to the bottom of the inner cavity of the infusion tube (120); Multiple stop blocks (220) are set in pairs, and two groups are set up. The two stop blocks (220) in each group are vertically symmetrical about the stop block (210). The two stop blocks (210) and the two groups of stop blocks (220) are staggered.

2. The culture device for endometrial cancer organoids according to claim 1, characterized in that, The infusion tube (120) is provided with a measuring mechanism (300) on the outside. The measuring mechanism (300) includes a micro pump (310) connected and communicating with the measuring cylinder (140), a thin tube (320) connected to the extraction end of the micro pump (310), a reservoir (330) sleeved on the end of the thin tube (320), and a solenoid valve (340) installed on the infusion tube (120).

3. The culture device for endometrial cancer organoids according to claim 2, characterized in that, A mounting base (400) is connected between the measuring cylinder (140) and the micro pump (310), and the mounting base (400) has an opening located on the front side of the measuring cylinder (140).

4. The culture device for endometrial cancer organoids according to claim 3, characterized in that, A cross platform (500) is installed at the bottom of the mounting base (400), and the bottom of the cross platform (500) is fixedly connected to the top of the liquid storage cylinder (330).

5. A culture device for endometrial cancer organoids according to claim 4, characterized in that, Multiple reinforcing ribs (600) are connected between the liquid storage cylinder (330) and the cross platform (500), and the multiple reinforcing ribs (600) are evenly spaced and arranged in a ring.

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