Culture vessel

Abstract

To provide a culture vessel that makes it possible to efficiently perform the task of moving a culture medium.SOLUTION: A culture vessel is used while being accommodated in a housing part of a support member. The culture vessel includes: a cylindrical main body with a first cut-out and a second cut-out positioned opposite each other at the upper end; and first and second support parts that are disposed opposite the upper end of the main body and hold the culture vessel in the support member.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a culture vessel. [Background technology]

[0002] Conventionally, as disclosed in Patent Document 1, cells have been cultured using a culture vessel having a container for culturing cells.

[0003] Such a culture vessel includes an outer culture vessel and an inner culture vessel, which is a so-called insert vessel. The outer culture vessel has a cylindrical outer container. The inner culture vessel has a cylindrical inner container. The inner culture vessel is supported by the outer culture vessel by four brim-shaped supports provided at the upper end while being contained in the container. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Publication No. 7-75553 Summary of the Invention [Problem to be solved by the invention]

[0005] When culturing cells using the above-mentioned culture vessel, the culture medium may be transferred between the outer container of the outer culture vessel and the inner container of the inner culture vessel. The culture medium may be transferred using a pipette, for example.

[0006] Incidentally, in the case of the culture vessel disclosed in Patent Document 1 as described above, it may be difficult to insert a pipette into the outer housing part when moving the culture medium. Also, in the case of the culture vessel disclosed in Patent Document 1, when moving the pipette between the inner housing part and the outer housing part during the work of moving the culture medium, the upper end of the inner culture vessel may get in the way of the movement of the pipette.

[0007] An object of the present invention is to provide a culture vessel that enables efficient transfer of a culture medium. [Means for solving the problem]

[0008] One aspect of the culture vessel according to the present invention is A culture vessel used in a state housed in a housing part of a support member, A cylindrical main body portion having a first notch and a second notch opposed to each other at an upper end portion; The support member includes a first support portion and a second support portion that are disposed opposite each other at the upper end portion of the main body portion and support the culture vessel on the support member. Effect of the Invention

[0009] According to the present invention, a culture vessel capable of efficiently transferring a culture medium can be provided. [Brief description of the drawings]

[0010] [Figure 1] FIG. 1 is a perspective view of a culture vessel set according to an embodiment. [Figure 2A] FIG. 2A is a plan view of the inner culture vessel. [Figure 2B] FIG. 2B is a plan view of the inner culture vessel for explaining a first region, a second region, a third region, and a fourth region of the inner culture vessel. [Diagram 3] FIG. 3 is a bottom view of the inner culture vessel. [Figure 4] FIG. 4 is a side view of the inner culture vessel shown in FIG. 2A as viewed in the direction indicated by the arrow A1 in FIG. 2A. [Diagram 5] FIG. 5 is a side view of the inner culture vessel shown in FIG. 2A as viewed in the direction indicated by the arrow A2 in FIG. 2A. [Figure 6] FIG. 6 is a cross-sectional view of the culture vessel in use. [Figure 7] FIG. 7 is a plan view of the culture vessel in use. [Figure 8] FIG. 8 is a cross-sectional view of the culture vessel in use. [Figure 9]FIG. 9 is a cross-sectional view of the culture vessel in use. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0011] Hereinafter, the culture vessel according to the present invention will be described in detail with reference to the drawings. Note that the culture vessel described below is one example of the culture vessel according to the present invention, and the present invention is not limited to the embodiment described below.

[0012] [Embodiment] A culture vessel according to an embodiment of the present invention will be described with reference to FIGS. 1 to 8.

[0013] 1, a culture vessel 1 is used, for example, for culturing cells derived from a human. Such a culture vessel 1 has a storage space for storing cells (specifically, an outer storage section 223 and an inner storage section 35 described below). The culture vessel 1 is stored in the culture space of a culture device (for example, an incubator) with cells to be cultured (hereinafter referred to as "target cells") stored in the storage space.

[0014] The culture vessel 1 of this embodiment can be used, for example, to culture spheroids (cell aggregates) of target cells. The culture vessel 1 does not need to be used in a state housed in a culture device. The culture vessel 1 may be used in various situations depending on the target cells. The shape of the culture vessel is not limited to the shape of the culture vessel 1.

[0015] The following describes a specific configuration of the culture vessel 1. In the following description, unless otherwise specified, when the words "inside" or "outside" are used, the "inside" refers to the inside in the radial direction of the culture vessel 1 and the members that constitute the culture vessel 1. The "outside" refers to the outside in the radial direction of the culture vessel 1 and the members that constitute the culture vessel 1.

[0016] The culture vessel 1 has an outer culture vessel 2 and an inner culture vessel 3 .

[0017] The outer culture vessel 2 corresponds to an example of a support member, and has a frame portion 21 and a plurality of outer culture portions 22.

[0018] The frame 21 is, for example, rectangular in shape, and supports a plurality of outer culture sections 22. The frame 21 is, for example, made of synthetic resin. Examples of synthetic resins constituting the frame 21 include polystyrene and polyolefin. The polyolefin may be a cyclic olefin (co)polymer, a 4-methyl-1-pentene (co)polymer, polypropylene, or polyethylene. From the viewpoint of gas permeability, a 4-methyl-1-pentene (co)polymer is preferred. Also, from the viewpoint of heat resistance, polystyrene, polypropylene, or a cyclic olefin (co)polymer is preferred.

[0019] The outer culture section 22 has a tubular section 221 and a bottom section 222 (see Figs. 6 to 8). The tubular section 221 is cylindrical and has an open upper end. The bottom section 222 closes the lower end of the tubular section 221. The outer culture section 22 has an outer storage section 223 defined by the inner circumferential surface of the tubular section 221 and the upper surface of the bottom section 222.

[0020] The outer accommodating section 223 accommodates the inner culture vessel 3 in the usage state (see Figs. 6 to 8). Furthermore, the outer accommodating section 223 accommodates target cells and / or culture fluid, etc. in the usage state. The outer culture sections 22 have the same configuration. The shape of the outer culture section is not limited to the shape of the outer culture section 22 described above.

[0021] The inner culture vessel 3 is used in a state of being accommodated in the outer accommodation section 223 of the outer culture vessel 2. Such an outer accommodation section 223 can also be regarded as a culture vessel. Also, the outer culture vessel 2 (specifically, the outer culture section 22) and the inner culture vessel 3 can also be regarded as a culture vessel. The culture vessel 1 can be provided with a maximum of the same number of inner culture vessels 3 as the number of outer culture sections 22 (six in this embodiment). The number of outer culture sections 22 is not limited to six. For example, the number of outer culture sections 22 may be 24 or 96. Of course, the number of outer culture sections 22 may be other than 6, 24, and 96.

[0022] The inner culture vessel 3 has a main body portion 31 , a support portion 32 , and a bottom portion 33 .

[0023] The main body 31 is cylindrical. The main body 31 is made of, for example, a synthetic resin. Examples of the synthetic resin constituting the main body 31 include polystyrene and polyolefin. The polyolefin may be a cyclic olefin (co)polymer, a 4-methyl-1-pentene (co)polymer, polypropylene, or polyethylene. From the viewpoint of gas permeability, a 4-methyl-1-pentene (co)polymer is preferable. Also, from the viewpoint of heat resistance, polystyrene, polypropylene, or a cyclic olefin (co)polymer is preferable.

[0024] The total light transmittance of the main body 31 is 80% or more and 99% or less. The total light transmittance is measured in accordance with the test method specified in JIS K 7218.

[0025] The main body 31 is a truncated cone cylinder whose outer and inner diameters decrease toward the bottom. Therefore, the outer peripheral surface of the main body 31 is a truncated cone surface whose outer diameter decreases toward the bottom. The inner peripheral surface of the main body 31 is also a truncated cone surface whose outer diameter decreases toward the bottom. This configuration can ensure a large culture space between the lower end of the outer peripheral surface of the main body 31 and the inner peripheral surface of the inner culture vessel 3.

[0026] Moreover, the outer peripheral surface of the main body part 31 is smooth all around. In other words, no protrusions or recesses are provided on the outer peripheral surface of the main body part 31. Such a configuration of the outer peripheral surface of the main body part 31 contributes to improving the manufacturing efficiency of the inner culture vessel 3.

[0027] The main body 31 has a first notch 311a and a second notch 311b at two positions on the upper end portion. The first notch 311a and the second notch 311b face each other in the radial direction of the main body 31.

[0028] The first notch 311a is provided in a first region 34a at the upper end of the main body 31. The first region 34a is a region shaded in black in Fig. 2B when the main body 31 is seen in a plan view.

[0029] The second cutout portion 311b is provided in a second region 34b at the upper end portion of the main body portion 31. The second region 34b is a region marked with diagonal grid in Fig. 2B when the main body portion 31 is viewed from above.

[0030] Therefore, the first region 34a and the second region 34b face each other in the radial direction of the main body 31. The direction in which the first region 34a (in other words, the first notch 311a) and the second region 34b (in other words, the second notch 311b) face each other is also referred to as a first direction (see FIG. 2B). 31 The direction perpendicular to the direction parallel to the center axis O of the main body 31 is referred to as the second direction (see FIG. 2B). 31 , and have a point-symmetric relationship with respect to

[0031] The circumferential length of the first region 34a (in other words, the first notched portion 311a) is 10% to 20% (preferably 15% to 18%) of the circumferential length of the upper end face of the main body portion 31. Unless otherwise specified, the circumferential direction refers to the circumferential direction of the main body portion 31. The circumferential length of the upper end face of the main body portion 31 refers to the circumferential length of the upper end face of the main body portion 31 in the plan view shown in FIG. 2B.

[0032] The circumferential length of the second region 34b (in other words, the second cutout portion 311b) is 10% to 20% of the circumferential length of the upper end face of the main body portion 31. Therefore, the circumferential lengths of the first region 34a (in other words, the first cutout portion 311a) and the second region 34b (in other words, the second cutout portion 311b) are 20% to 40% of the circumferential length of the upper end face of the main body portion 31.

[0033] 5, the first notch 311a and the second notch 311b have the same shape in side view. Specifically, the first notch 311a and the second notch 311b have a substantially rectangular shape in side view. 311 is the height H of the main body 31 31 The range is from 10% to 30% (preferably 17%) of the total.

[0034] The support part 32 is a part for supporting the inner culture vessel 3 on the outer culture vessel 2 (specifically, the outer culture section 22).

[0035] The first support portion 321 is flange-shaped and extends from an upper end portion of the main body portion 31 outward in the radial direction of the main body portion 31. The upper and lower surfaces of the first support portion 321 are flat surfaces parallel to the horizontal direction (see FIG. 5).

[0036] The second support portion 322 is flange-shaped and extends from the upper end of the main body portion 31 radially outward of the main body portion 31. The upper and lower surfaces of the second support portion 322 are flat surfaces parallel to the horizontal direction (see FIG. 5). The first support portion 321 and the second support portion 322 extend from the upper end of the main body portion 31 in opposite directions to each other.

[0037] The shape of the first support portion 321 and the shape of the second support portion 322 are determined based on the central axis O of the main body portion 31. 31 The protrusion amounts L1 and L2 (see FIG. 2A) of the first support portion 321 and the second support portion 322 from the upper end portion of the main body portion 31 are respectively set to the outer diameter D 31 In this embodiment, the outer diameter D of the upper end of the main body 31 is 15% or more and 25% or less. 31 is 13.5 mm. However, the outer diameter D 31 does not have to be limited to 13.5 mm. In the present embodiment, the protrusion amounts L1 and L2 of the first support portion 321 and the second support portion 322 are 3 mm. The protrusion amounts L1 and L2 of the first support portion 321 and the second support portion 322 do not have to be limited to 3 mm.

[0038] In addition, the protrusion amounts L1 and L2 (see FIG. 2A) of the first support portion 321 and the second support portion 322 from the upper end portion of the main body portion 31 are the radial movement amount L that the inner culture vessel 3 can move in the outer storage portion 223 of the outer culture section 22 when the culture vessel 1 is in use (see FIGS. 6 to 8). a In the state shown in FIG. 6, the central axis O3 of the inner culture vessel 3 and the central axis O of the outer culture section 22 are 22 are located on the same axis.

[0039] Such a configuration can prevent the inner culture vessel 3 from falling off from the outer culture section 22. Fig. 8 is a diagram showing a state in which the inner culture vessel 3 has moved to its maximum extent in the radial direction within the outer accommodation section 223 of the outer culture section 22. As shown in Fig. 8, even when the inner culture vessel 3 has moved to its maximum extent within the outer accommodation section 223, the inner culture vessel 3 does not fall off from the outer culture section 22. Thus, in this embodiment, the shapes of the first support section 321 and the second support section 322 are devised to prevent the inner culture vessel 3 from falling off.

[0040] Width W of the base end of the first support portion 321 and the second support portion 322 1a , W 2a (See FIG. 2A) is the inner diameter d 31 The outer diameter D of the upper end of the main body 31 is larger than that of the upper end of the main body 31. 31 Less than.

[0041] The width W of the base end of the first support portion 321 and the second support portion 322 1a , W 2a is the outer diameter D of the upper end of the main body 31 31 In this embodiment, the width W of the base end of the first support portion 321 and the second support portion 322 is 80% or more and 95% or less. 1a , W 2a However, the width W of the base end of the first support portion 321 and the second support portion 322 is 11.8 mm. 1a , W 2a In the present embodiment, the outer diameter D of the upper end of the main body 31 is not necessarily limited to 11.8 mm. 31 is 13.5 mm. However, the outer diameter D31 does not need to be limited to 13.5 mm.

[0042] Moreover, the first support portion 321 and the second support portion 322 are each substantially trapezoidal in plan view (see FIG. 2A). Specifically, the width of the first support portion 321 and the second support portion 322 decreases as they move away from the main body portion 31.

[0043] Width W of the tip of the first support portion 321 and the second support portion 322 1b , W 2b (See FIG. 2A) is the inner diameter d 31 Specifically, the width W of the tip of the first support portion 321 and the second support portion 322 is smaller than the width W of the tip of the first support portion 321 and the second support portion 322. 1b , W 2b is the inner diameter d of the upper end of the main body 31 31 In this embodiment, the width W of the tip of the first support portion 321 and the second support portion 322 is 75% or more and 85% or less. 1b , W 2b However, the width W of the tip of the first support portion 321 and the second support portion 322 is 9 mm. 1b , W 2b does not have to be limited to 9mm.

[0044] In addition, the width W of the tip of the first support portion 321 and the second support portion 322 1b , W 2b is the width W of the base end of the first support portion 321 and the second support portion 322 1a , W 2a In this configuration, the width W 1b , W 2b In this embodiment, the width W of the tip of the first support portion 321 and the second support portion 322 is large. 1b , W 2b However, the width W of the tip of the first support portion 321 and the second support portion 322 is 11.8 mm. 1b , W 2b does not need to be limited to 11.8 mm.

[0045] Width W of the tip of the first support portion 321 and the second support portion 322 1b , W 2b 8, even when the inner culture vessel 3 is moved to its maximum radial position within the outer accommodation section 223 of the outer culture section 22, a large contact area between the first support section 321 and the second support section 322 and the outer culture section 22 can be ensured. Therefore, even in the state shown in FIG. 8, the inner culture vessel 3 is stably supported relative to the outer culture vessel 2.

[0046] The tip edges of the first support portion 321 and the second support portion 322 are arc-shaped along the upper end edge of the outer culture portion 22. This configuration also makes it possible to increase the contact area between the first support portion 321 and the second support portion 322 and the outer culture portion 22.

[0047] The first support portion 321 is provided in a third region 34c at the upper end portion of the main body portion 31. The third region 34c is a region that is roughly hatched in Fig. 2B when the main body portion 31 is viewed in plan.

[0048] The second support portion 322 is provided in a fourth region 34d at the upper end portion of the main body portion 31. The fourth region 34d is a region that is finely hatched in Fig. 2B when the main body portion 31 is viewed in plan.

[0049] The third region 34c (in other words, the first support portion 321) and the fourth region 34d (in other words, the second support portion 322) face each other in the radial direction (in other words, the second direction) of the main body portion 31. The third region 34c (in other words, the first support portion 321) and the fourth region 34d (in other words, the second support portion 322) are arranged on the central axis O of the main body portion 31. 31 , and have a point-symmetric relationship with respect to

[0050] The third region 34c is a region between a first end of the first region 34a and a second end of the second region 34b in the circumferential direction. Therefore, the first support portion 321 is provided between the first end of the first notch 311a and the second end of the second notch 311b in the circumferential direction.

[0051] The first ends of the first region 34a, the second region 34b, the third region 34c, and the fourth region 34d are ends on the clockwise side in Fig. 2B, while the second ends of the first region 34a, the second region 34b, the third region 34c, and the fourth region 34d are ends on the opposite side to the clockwise direction in Fig. 2B.

[0052] The fourth region 34d is a region between the second end of the first region 34a and the first end of the second region 34b in the circumferential direction. Therefore, the second support portion 322 is provided between the second end of the first notch 311a and the first end of the second cutout portion 311b in the circumferential direction.

[0053] The circumferential length of the third region 34c is 30% to 40% of the circumferential length of the upper end face of the main body portion 31. The circumferential length of the fourth region 34d is 30% to 40% of the circumferential length of the upper end face of the main body portion 31. Thus, the circumferential lengths of the third region 34c and the fourth region 34d are 60% to 80% of the circumferential length of the upper end face of the main body portion 31.

[0054] The circumferential length of the third region 34c and the fourth region 34d, which are the portions where the first support portion 321 and the second support portion 322 are provided at the upper end of the main body portion 31, is greater than the circumferential length of the first region 34a and the second region 34b, which are the portions where the first notch portion 311a and the second notch portion 311b are provided at the upper end of the main body portion 31. This configuration can increase the width of the first support portion 321 and the second support portion 322. As a result, when the culture vessel 1 is in use (see Figs. 6 to 8), it is possible to suppress the inclination of the inner culture vessel 3 relative to the outer culture vessel 2. Therefore, the inner culture vessel 3 is supported in a stable state relative to the outer culture vessel 2.

[0055] The bottom 33 is an example of a microporous film and has a circular sheet shape. The bottom 33 closes the lower end of the main body 31. In other words, the bottom 33 closes the lower opening of the main body 31. The bottom 33 is a so-called track-etched membrane, and is a polyethylene terephthalate resin film. The bottom 33 has a plurality of micropores (not shown). Such a bottom 33 is fixed to the lower end surface of the main body 31 by an adhesive.

[0056] Thickness dimension W of bottom 33 33 is 5 um or more and 15 um or less. Bottom 33 has liquid permeability. Bottom 33 has a porosity of 3% or more and 18% or less. The porosity is the ratio of the area of ​​the micropores to the area of ​​bottom 33. The area of ​​bottom 33 and the area of ​​the micropores refer to the area of ​​bottom 33 and the area of ​​the micropores in a plan view. A plan view refers to viewing main body 31 from above and below.

[0057] The space defined by the inner circumferential surface of the main body 31 and the bottom 33 is the inner accommodating section 35. When the culture vessel 1 is in use (see FIGS. 6 to 8), the inner accommodating section 35 accommodates target cells and culture fluid. The culture fluid accommodated in the inner accommodating section 35 can move to the outer accommodating section 223 through the fine pores in the bottom 33. In addition, the culture fluid accommodated in the outer accommodating section 223 can move to the inner accommodating section 35 through the fine pores in the bottom 33.

[0058] A method for using the culture vessel 1 of this embodiment will be described below with reference to Figures 6 to 8. In addition to the description of the method for using the culture vessel 1 of this embodiment, the actions and effects obtained from the culture vessel 1 of this embodiment will also be described.

[0059] 6 is a cross-sectional view of the culture vessel 1 in a state in which the culture vessel 1 is in use. As shown in FIG. 6, the main body portion 31 of the inner culture vessel 3 is accommodated in the outer accommodating portion 223 of the outer culture portion 22 in the outer culture vessel 2.

[0060] The bottom 33 of the inner culture vessel 3 is disposed higher than the bottom 222 of the outer culture vessel 2. The bottom 33 of the inner culture vessel 3 and the bottom 222 of the outer culture section 22 are spaced apart by a predetermined distance.

[0061] As shown in Fig. 6, a culture solution 50 is accommodated in the inner accommodation section 35 of the inner culture vessel 3 and the outer accommodation section 223 of the outer culture section 22. Furthermore, a target cell 51 is accommodated in the inner accommodation section 35 of the inner culture vessel 3. A target cell (not shown) may also be accommodated in the outer accommodation section 223 of the outer culture section 22. The target cell 51 accommodated in the inner accommodation section 35 and the target cell accommodated in the outer accommodation section 223 may be different cells or may be the same cell. Incidentally, the target cell does not have to be accommodated in the inner accommodation section 35 or the outer accommodation section 223.

[0062] When the culture vessel 1 is in use, the first support part 321 and the second support part 322 of the inner culture vessel 3 are placed on the upper end surface 224 of the tubular part 221 of the outer culture section 22. In this state, openings 11a and 11b are provided at two positions in the circumferential direction between the upper end of the inner culture vessel 3 and the upper end of the outer culture section 22.

[0063] The openings 11a, 11b are provided at positions corresponding to the first notch 311a and the second notch 311b in the circumferential direction. As shown in Fig. 9, an operator performing cell culture work can insert the pipettes 4a, 4b into the outer storage section 223 through the openings 11a, 11b. In the case of this embodiment, since the first notch 311a and the second notch 311b are provided at positions corresponding to the openings 11a, 11b of the inner culture vessel 3, even if the pipettes 4a, 4b are tilted, the pipettes 4a, 4b are unlikely to interfere with the inner culture vessel 3. As a result, the operator can efficiently perform cell culture work.

[0064] 9 passes through the position of pipette 4a1 to the position of pipette 4a2. At this time, since the first notch 311a is provided at a position corresponding to the opening 11a of the inner culture vessel 3, the tip of the pipette 4a is unlikely to interfere with the inner culture vessel 3. As a result, the operator can efficiently perform the cell culture work. [Industrial Applicability]

[0065] The culture vessel according to the present invention can be applied to the culture of various cells. [Explanation of symbols]

[0066] 1 Culture vessel 11a, 11b opening 2 Outer culture vessel 21 Frame 22 Outer culture section 221 Cylinder part 222 Bottom 223 Outer storage section 224 Top surface 3 Inner culture vessel 31 Main body 311a First cutout 311b Second notch 32 Support part 321 First support part 322 Second support part 33 Bottom 34a First area 34b Second area 34c Third Realm 34d Fourth basin 35 Inner storage compartment 4a, 4b, 4a1, 4a2 Pipettes 50 Culture medium 51 Target Cells

Claims

1. A culture vessel used in a state housed in a housing part of a support member, A cylindrical main body portion having a first notch and a second notch opposed to each other at an upper end portion; A first support part and a second support part are arranged opposite to each other at an upper end part of the main body part and support the culture vessel on the support member. Culture container.

2. The first support portion and the second support portion are provided between the first notch portion and the second notch portion. The culture vessel according to claim 1 .

3. The first support portion and the second support portion are flange-shaped and extend in opposite directions from the upper end portion. The culture vessel according to claim 1 .

4. A width of a base end of the first support portion and the second support portion is larger than an inner diameter of the upper end portion and smaller than an outer diameter of the upper end portion. The culture vessel according to claim 1 .

5. A length of a portion at the upper end portion where the first support portion and the second support portion are provided is 60% or more and 80% or less of a circumferential length of the upper end portion. The culture vessel according to claim 1 .

6. The shape of the first notch portion and the shape of the second notch portion are symmetrical, The depth of the first notch and the second notch is 10% or more and 30% or less of the height of the main body. The culture vessel according to claim 1 .

7. A length of a portion of the upper end portion where the first notch portion and the second notch portion are provided is 20% to 40% of a circumferential length of the upper end portion. The culture vessel according to claim 1 .

8. A length in the circumferential direction of the upper end portion of a portion at which the first support portion and the second support portion are provided at the upper end portion is greater than a length in the circumferential direction of a portion at which the first notch portion and the second notch portion are provided at the upper end portion. The culture vessel according to claim 1 .

9. The first support portion and the second support portion are symmetrical brim-shaped. A protrusion amount of the first support portion and the second support portion from the upper end portion is 30% to 50% of an outer diameter of the upper end portion. The culture vessel according to claim 1 .

10. The first support portion and the second support portion are each flange-shaped, and a width of a base end portion is 80% or more and 95% or less of an outer diameter of the upper end portion. The culture vessel according to claim 1 .

11. the first support portion and the second support portion each have a flange shape and are placed on the support member in a usage state; a protruding amount of the first support portion and the second support portion from the upper end portion is greater than a movable amount of the main body portion within the storage portion in the usage state; The culture vessel according to claim 1 .

12. The main body contains polystyrene resin or cyclic polyolefin resin. The culture vessel according to claim 1 .

13. The total light transmittance of the main body measured in accordance with the test method specified in JIS K 7218 is 80% or more and 99% or less. The culture vessel according to claim 1 .

14. a microporous film closing a lower opening of the body portion; The porosity of the microporous film is 3% or more and 18% or less. The culture vessel according to claim 1 .

15. The microporous film is a polyethylene terephthalate resin film having a thickness dimension of 5 um or more and 15 um or less. The culture vessel according to claim 14.

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