The invention relates to the field of otolaryngology medical equipment, in particular to tympanocentesis and intratympanic injection medical equipment, and mainly aims at solving the technical problem that in the prior art, medicine backflow is caused by a secondary hole formed when a needle is inserted to inject medicine for the second time after puncture and aspiration, and puncture pain of a patient is increased. According to the adopted technical scheme, the tympanocentesis and intratympanic injection medical equipment comprises a supporting frame, a fixing device, a connecting block and a tympanocentesis and intratympanic injection medical device; the tympanocentesis and intratympanic injection medical device comprises a first puncture device, a second puncture device, a suction device and an injection device, the first puncture device comprises a puncture needle and a first connector, the second puncture device comprises an injection needle and a second connector, the suction device comprises a negative pressure mechanism and a third connector, and the injection device comprises an injector and a fourth connector. The tympanocentesis and intratympanic injection medical device has a suction state and an injection state.