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The application provides a composition containing prothioconazole, clothianidin and mefenoxam and application thereof. The dosage form of the composition is a suspension seed coating agent, and the suspension seed coating agent contains the following ingredients in the weight percentage: 0.5-3% of prothioconazole, 5-15% of clothianidin, 0.5-3% of mefenoxam, 4-16% of wetting dispersant, 0.2-2% of thickening agent, 2-8% of film forming agent, 0.1-0.5% of preservative, 1-6% of antifreeze, 0.2-1.2% of defoaming agent, 0.1-0.5% of pigment and deionized water to make up 100%. The suspension seed coating agent provided by the application has excellent preparation performance, can significantly improve the control effect of the pesticide on pests and diseases, can effectively reduce the use dosage of the active ingredient, can reduce environmental pollution and can ensure food safety.