The invention relates to a method for preparing
bovine serum albumin hybridization membrane, belonging to the technical field of biological-organic hybridization. The preparation technique comprises preparation of
powder hybridization membrane and layer-by-layer assembled hybridization membrane. In preparing layer-by-layer assembled hybridization membrane consisting of multilayer packaging
protein and houghite nanoplates, the
protein and the houghite form membrane in an alternating way in the layer-by-layer assembled hybridization membrane; characteristic spectrum intensity of the
protein increases in a linear way along with the increase of assembled
layers; the houghite plates are arranged on the surface of the substrate in a compact way,
membrane structure of ovine
serum albumin hybridization membrane prepared by layer-by layer
assembly is even and continuous, the spectral intensity of the ovine
serum albumin increases in a linear way along with the increase of assembled
layers; the surface of the hybridization membrane is compact and smooth, the protein is evenly distributed on the surface of the membrane. The method has the advantages that the method for simultaneous preparation is easy in that no complex device is needed, the preparation cost is low, water is the only
solvent in the preparation process and the preparation method is environment-friendly.