37results about How to "Suitable for large-scale operations" patented technology

The invention discloses an incomplete antibody screening colloidal gold reagent kit and a preparation method thereof. The reagent kit comprises a first erythrocyte membrane antigen gold particle solution, a second erythrocyte membrane antigen gold particle solution, a third erythrocyte membrane antigen gold particle solution and an antihuman globulin solution, wherein an antigen spectrum of each of the first erythrocyte membrane antigen gold particle solution, the second erythrocyte membrane antigen gold particle solution and the third erythrocyte membrane antigen gold particle solution collectively include D, C, E, c, e, Jka, JKb, M, N, S, s, Fya, Fyb, Dia, K, k, Lea, Leb and P1 antigens. The reagent kit is long in storage time and easy to operate, the complicated process such as centrifuging, washing, and incubation is not needed, and a result is clear and easy to judge. The reagent kit also has the advantage of being suitable for large-scale operation and standard operation.

The invention provides a method for quickly building a plasma DNA sequencing library. The method includes the following steps: 1), extracting plasma DNA; 2), performing end filling on the plasma DNA to obtain plasma DNA subjected to end filling; 3), performing 3' suspension A on the plasma DNA subjected to end filling to obtain plasma DNA after 3' suspension A; 4), connecting the plasma DNA after 3' suspension A with a sequencing joint to obtain a connection product; 5), purifying the connection product to obtain a purified product. The invention further provides a kit for building the plasma DNA sequencing library. Building process of the plasma DNA sequencing library is simplified greatly, pollution of environment and pollution among samples due to PCR amplification is removed, requirements, on environment, of building of the plasma DNA library are lowered, nonuniformity in genome covering caused by the process of PCR is avoided, building of the plasma sample library is enabled to be lower in cost, higher in efficiency and quick, and plasma DNA sequencing results are truer and more effective.

The invention discloses a transfer plasmid for transforming Acinetobacter baumannii, which comprises, in clockwise order, a replicon ori, an ampicillin resistance gene AmpR, a transposon sequence, a junction transfer initiation site oriT, the transposon sequence contains a base sequence capable of autonomously emitting Acinetobacter baumannii and a resistance gene, and both ends of the resistancegene are provided with DifR and DifL sequences; also disclosed is an autonomously illuminated Acinetobacter baumannii without a resistance marker, the gene of Acinetobacter baumannii containing a genecapable of expressing autonomous light-emitting protein, and no resistance screening gene is in the genome of Acinetobacter baumannii. In the present invention, the autonomously illuminated Acinetobacter baumannii, which is constructed by the transfer plasmid pUC18T-mini-Tn7T-lux-Ab-dif-Apr and the auxiliary plasmid pTNS3, can emit light without adding any substrate; and luminescent colonies canbe seen with the naked eye in a dark environment.

The invention relates to a spherical lithium ion battery anode material and a preparation method of a precursor thereof. The preparation method comprises the following steps of: with newly-prepared nano flaky nickel hydroxide as a raw material, dispersing the raw material in an aqueous solution, adding a certain proportion of cobalt salt into the aqueous solution, reacting for several hours in an enclosed vessel to obtain uniform spherical granules which can be used as a precursor for preparing the lithium ion battery anode material; and uniformly mixing the obtained precursor granules and a lithium salt and carrying out high-temperature calcining to finally obtain the spherical lithium ion battery anode material with uniform granule size. The preparation method disclosed by the invention is simple to operate, high in efficiency, easy in obtainment of raw material, low in cost and environment-friendly; and the prepared spherical lithium battery anode material has good electrochemical property.

The invention discloses an integrative plasmid pOPHI and a resistance screening marker-free self-luminescent mycobacterium. The integrative plasmid pOPHI comprises a promoter, an enzyme gene (LuxCDABE) needed for luminescence, a fusion gene of an integrase gene (Int) and a resistance screening gene, direct repeat sequences DifR and DifL positioned on two ends of the fusion gene, a phage integration site (attP) and a replicon. After the integrative plasmid pOPHI is transferred into the mycobacteria, a photobacterium of which the resistance gene and the integrase gene are lost is screened out by subculturing, and the photobacterium is the resistance screening marker-free self-luminescent mycobacterium. Whether the resistance screening marker-free self-luminescent mycobacterium obtained by construction is dead or not is judged according to whether the bacterium is luminescent or not, the mycobacterium can be used for a plurality of detections of experiments, and credible experimental data can be quickly obtained.

The invention discloses a reciprocating kiwifruit slicing device which is characterized by comprising a first cutter assembly and a second cutter assembly; upper sliding strip bulges are embedded into clearances among adjacent lower sliding strip bulges; the lower sliding strip bulges are embedded into clearances among the adjacent upper sliding strip bulges; the cutting edges of a first group of blades are in flush with those of a second group of blades; a left upper sliding strip and a left lower sliding strip are positioned in a left sliding groove; a right upper sliding strip and a right lower sliding strip are positioned in a right sliding groove; the upper sliding strip bulges are in clearance fit with the adjacent lower sliding strip bulges; the upper sliding strip bulges and the adjacent lower sliding strip bulges can oppositely slide along the direction of the cutting edges; guide holes being opposite to all threaded holes are formed in the outer side walls of the left and right sliding grooves; and guide columns connected with the threaded holes penetrate through the guide holes. By adoption of the reciprocating kiwifruit slicing device, the kiwifruit can be cut into kiwifruit slices being uniform in thickness, and the possibility of kiwifruit deformation can be lowered.

The invention, which belongs to the field of agricultural machines, relates to a dig-pull type cassava harvester, which comprises a chassis (1), the front part of the chassis is provided with a suspension (4), the lower part of the chassis is connected with a digger blade (2), a clamping conveyer (5), the front end of which is low and the rear end of which is high, is arranged on the chassis, andthe clamping conveyer is connected with a power source of a tractor through a transmission. The cassava harvester can utilize the digger blade and the clamping conveyer to dig and harvest cassavas atthe same time, and thereby is characterized by labor saving, easy harvesting, high efficiency, high speed, high automation degree and large-scale operation.

The invention discloses an immobilized recombinant penicillin G acylase and an application thereof to preparation of (S)-2-aryl-amino acid and cephalosporin antibiotics. The immobilized recombinant penicillin G acylase disclosed by the invention is capable of converting an S-type substrate into an S-type product within shorter time, high in substrate tolerance and strict in S selectivity for the substrate and structural analogues thereof; due to the addition of cobalt ions as well as a protective agent with phenylacetic acid and glycerin as enzyme active centers, the immobilized recombinant penicillin G acylase disclosed by the invention is prevented from being successfully subjected to multipoint covalent immobilization on the surface of an epoxy resin carrier under the condition that enzyme molecules are seriously inactivated in an immobilization process, and the immobilization method is simple and feasible, cheap in raw materials and suitable for large-scale operation; the immobilized recombinant penicillin G acylase can be used for synthesizing cephalosporin antibiotics and splitting the prepared (S)-2-aryl-amino acid, and can be continuously used for more than 50 batches before the activity of the immobilized enzyme is not remarkably reduced.

The invention discloses an incomplete antibody screening colloidal gold reagent kit and a preparation method thereof. The reagent kit comprises a first erythrocyte membrane antigen gold particle solution, a second erythrocyte membrane antigen gold particle solution, a third erythrocyte membrane antigen gold particle solution and an antihuman globulin solution, wherein an antigen spectrum of each of the first erythrocyte membrane antigen gold particle solution, the second erythrocyte membrane antigen gold particle solution and the third erythrocyte membrane antigen gold particle solution collectively include D, C, E, c, e, Jka, JKb, M, N, S, s, Fya, Fyb, Dia, K, k, Lea, Leb and P1 antigens. The reagent kit is long in storage time and easy to operate, the complicated process such as centrifuging, washing, and incubation is not needed, and a result is clear and easy to judge. The reagent kit also has the advantage of being suitable for large-scale operation and standard operation.

The invention discloses a cement cleaning agent as well as preparation and application methods thereof. The cement cleaning agent consists of a solution A, a solution B and a solution C, the solution A is prepared from the following components in percentage by mass: 16 to 32 parts of nano magnesium hydroxide, 15 to 26 parts of cationic surface active agent and 942 to 969 parts of water; the solution B is prepared from 240 to 320 parts of organic acid, 180 to 260 parts of inorganic acid, 98 to 127 parts of dispersing agent, 8 to 14 parts of complexing agent and 279 to 474 parts of water; the solution C is prepared from 980 to 988 parts of water and 12 to 20 parts of urea. The cement cleaning agent can penetrate into hardened cement to efficiently remove thick cement residues adhered to the surface of a material, and meanwhile, the material is not damaged. The prepared cement cleaning agent is high in efficiency, good in effect, suitable for large-scale operation and good in adaptability, and has good social benefits and economic benefits.

The invention discloses a flue gas dust removal system, which comprises a cooling well and a flue gas conveying pipe; the cooling well is located above a foundation and extends upwards, wherein the upper end of the cooling well is provided with a transition chamber in a communicating mode, the flue gas conveying pipe penetrates through the transition chamber and extends into the cooling well, andan observation window is arranged on the outer side face of the transition chamber; a smoke washing box is fixedly arranged at the upper end of the transition chamber and comprises a curved flow channel and a liquid collecting tank, and two sets of air guide pipes are symmetrically arranged at the upper end of the transition chamber and extend upwards to the two sides of the top of the smoke washing box. According to the flue gas dust removal system, by designing the cooling well, compared with traditional cooling structures such as a coal economizer and a water cooler, the cooling well utilizes underground water, so that the use cost is low, the contact time is greatly prolonged, the cooling effect on high-temperature flue gas is better, dust particles in the flue gas can be directly absorbed, and certain self-purification capacity is achieved. The system is suitable for large-scale operation, is suitable for different working conditions, and shows a better application prospect.

The invention discloses a reciprocating Chinese gooseberry slitting device, which is characterized by comprising a first knife assembly, a second knife assembly, a third knife assembly and a fourth knife assembly; upper draw slip protrusions fall into gaps between adjacent lower draw slip protrusions, the lower draw slip protrusions fall into gaps between the adjacent upper draw slip protrusions, the cutting edges of a first blade set and a second blade set are flush, a left upper draw slip and a left lower draw slip are arranged in a left chute, a right upper draw slip and a right lower draw slip are arranged in a right chute, the upper draw slip protrusions and the adjacent lower draw slip protrusions are in clearance fit and can slide oppositely along the cutting edges, guide holes opposite to threaded holes are formed in the outer walls of the left and right chutes, and guide posts connected with the threaded holes penetrates through the guide holes. By adopting the device, the cut Chinese gooseberry slits are uniform in thickness, and the deformation probability of Chinese gooseberry can be reduced.

The invention relates to a spherical lithium ion battery anode material and a preparation method of a precursor thereof. The preparation method comprises the following steps of: with newly-prepared nano flaky nickel hydroxide as a raw material, dispersing the raw material in an aqueous solution, adding a certain proportion of cobalt salt into the aqueous solution, reacting for several hours in an enclosed vessel to obtain uniform spherical granules which can be used as a precursor for preparing the lithium ion battery anode material; and uniformly mixing the obtained precursor granules and a lithium salt and carrying out high-temperature calcining to finally obtain the spherical lithium ion battery anode material with uniform granule size. The preparation method disclosed by the invention is simple to operate, high in efficiency, easy in obtainment of raw material, low in cost and environment-friendly; and the prepared spherical lithium battery anode material has good electrochemical property.

The invention discloses a reciprocating Chinese gooseberry slitting device, which is characterized by comprising a first knife assembly, a second knife assembly, a third knife assembly and a fourth knife assembly; upper draw slip protrusions fall into gaps between adjacent lower draw slip protrusions, the lower draw slip protrusions fall into gaps between the adjacent upper draw slip protrusions, the cutting edges of a first blade set and a second blade set are flush, a left upper draw slip and a left lower draw slip are arranged in a left chute, a right upper draw slip and a right lower draw slip are arranged in a right chute, the upper draw slip protrusions and the adjacent lower draw slip protrusions are in clearance fit and can slide oppositely along the cutting edges, guide holes opposite to threaded holes are formed in the outer walls of the left and right chutes, and guide posts connected with the threaded holes penetrates through the guide holes. By adopting the device, the cut Chinese gooseberry slits are uniform in thickness, and the deformation probability of Chinese gooseberry can be reduced.

The invention discloses an application of ascorbic acid as a sleep release accelerating agent in breaking seed dormancy. According to the invention, a fact that ascorbic acid can break seed dormancy and can promote germination and the build of normal seedling form is found for the first time. Through the utilization of the method provided by the invention, the effect of breaking dormancy is the same as the effect of gibberellin, but the cost is low and no pollution is generated; compared with low-temperature stratification treatment, the method has greatly shortened time for breaking the seeddormancy, and the operation is convenient; and the guarantee of the emergence rate of seeds in conventional seedling breeding is facilitated. The method provided by the invention is simple and convenient to operate, is economical, practical and environmentally friendly, does not need special equipment, can be operated on a large scale, and can be demonstrated, promoted and applied to cultivation practice.

The present invention discloses a marine blue algae processing system and a processing method thereof. The system comprises a blue algae collector. The outlet end of the blue algae is orderly connected in series with a first grade stirring kettle, a gravitational separation machine, a second grade stirring kettle, a squeeze filter and a filter cake pool via pipes and valves. A three-grade processing method comprising primary condensation, secondary condensation and squeezing is adopted. The system is arranged on a running powerboat planarly or spatially according to the space of the boat. Thesystem uses the running speed of the boat to collect blue algae. The operating efficiency of processing blue algae can be quickly improved. Nuisance-free and reusable algae biscuits are formed. The operation is simple and convenient. The process flow system is highly effective and successive, and is suitable for large-scale operation.