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Application of anti-intercellular adhesion molecule-1 monoclonal antibody for preparing medicine for treating avian influenza

A technology of adhesion molecules and bird flu, applied in the direction of antibodies, antiviral agents, etc., can solve problems such as no treatment methods

Inactive Publication Date: 2011-05-11
MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the ability of highly pathogenic avian influenza to spread horizontally among animals and between humans, there is no effective prevention and control measure when the human avian influenza epidemic occurs, and there is no effective treatment after the onset

Method used

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  • Application of anti-intercellular adhesion molecule-1 monoclonal antibody for preparing medicine for treating avian influenza
  • Application of anti-intercellular adhesion molecule-1 monoclonal antibody for preparing medicine for treating avian influenza
  • Application of anti-intercellular adhesion molecule-1 monoclonal antibody for preparing medicine for treating avian influenza

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: Preparation of avian influenza mouse model

[0021] The purpose of this example is to prepare the experimental animal model required by the present invention.

[0022] Please see attached figure 1 , 2 , the experimental animals used in this study were clean-grade Balb / C mice, 18-20 g, purchased from Changchun Institute of Biological Products, license number SCXK-2002-0001; highly pathogenic avian influenza virus (H5N1 subtype) ( A / Tiger / Harbin / 01 / 2001), the virus allantoic fluid collected after SPF chicken embryo multiplication, hemagglutination titer (HA): 2 6 , mouse LD 50 for 10 -7.25 , provided by the virus laboratory of our institute. All experiments were carried out in a biosafety level III laboratory. ICAM-1 monoclonal antibody was self-made and purchased from American R&D Company. The purity of the antibody was 95%, and the concentration was 1 mL / mg; the titer was: 1:51200. Mouse ICAM-1 ELISA detection kit, rabbit anti-mouse ICAM-1 antibody a...

Embodiment 2

[0026] Example 2: Correlation between mouse lung inflammation caused by avian influenza virus and ICAM-1

[0027] 2.1 The purpose of this example is to explore the correlation between the mouse lung inflammation caused by avian influenza virus and ICAM-1 by sandwich ELISA method.

[0028] Please see attached image 3 , 120 experimental mice were randomly divided into 3 groups, randomly divided into normal control group, virus challenge group and post-challenge administration group, 40 mice in each group. To challenge the virus according to the method of Example 1, the post-challenge administration group was administered on the 1st, 3rd, and 5th days after the challenge, and each mouse was injected with 40 μg of ICAM-1 monoclonal antibody pure product through the tail vein (2 μg / g mouse) , a total of 3 times. From the first day to the eighth day after the challenge, 5 mice per group were killed every day, the lungs were ground with 1ml PBS buffer, the supernatant was collecte...

Embodiment 3

[0031] Example 3: Experimental Study of ICAM-1 Monoclonal Antibody Treating Avian Influenza Mice

[0032] The purpose of this example is to detect the therapeutic effect of anti-ICAM-1 monoclonal antibody on avian influenza mice.

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Abstract

The invention discloses an application with anti-intercellular sticking molecule-1 single antibody to prepare the medicine to treat fowl flu, which is characterized by the following: adopting H5N1 hypotype fowl flu mouse mode of anti-intercellular sticking molecule-1 single antibody of pure; setting the survival rate of treating group higher than matched group of virus; possessing distinctive difference in pathologic change of lung; displaying the miscellaneous index of the treating group higher than the matched group of virus with disease state, body weight of disease state and change of lung index; possessing distinctive treating action for fowl flu mouse without toxic side action for lung. This invention provides criterion for human and mammal fowl flu.

Description

technical field [0001] The present invention relates to the application of the antibody, specifically the application of the anti-intercellular adhesion molecule-1 monoclonal antibody as a medicine for treating bird flu. Background technique [0002] The frequent occurrence of highly pathogenic avian influenza (HPAIV) has seriously affected the development of poultry farming in my country and the world. At present, HPAIV has spread from Asia to Africa and Europe. At the same time, there are increasing reports of avian influenza viruses crossing birds and infecting mammals. The number of AIV-infected people is also increasing, and HPAIV poses a huge threat to human beings. AIV is likely to break through the interspecies barrier and spread among humans, leading to the pandemic or outbreak of human avian influenza. Therefore, preventing and controlling this possible spread is the most important task for the global prevention and control of avian influenza. Highly pathogenic a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/395A61P31/16
Inventor 岳玉环朱平夏咸柱杨松涛张国利吴广谋孙红万忠海
Owner MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
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