High-inorganic nitrogen-sourced biological compound fertilizer and preparation method thereof
A bio-compound fertilizer and inorganic nitrogen source technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve problems such as difficulty in unifying microbial activity and fertilizer efficiency
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Embodiment 1
[0021] Example 1 Preparation of Inorganic High Nitrogen-Resistant Bacteria
[0022] 1. Domestication of bacteria resistant to inorganic high nitrogen sources
[0023] Medium configuration:
[0024] Gao's No. 1 medium:
[0025] KNO 3 1.0g
[0026] NaCl 0.5g
[0027] K 2 HPO 4 0.5g
[0028] MgSO 4 7H 2 O 0.5g
[0029] FeSO 4 0.01g
[0030] Soluble starch 20g
[0031] Agar 20g
[0032] water 1000ml
[0033] PH7.2~7.4
[0034] Beef extract peptone medium:
[0035] Beef Extract 5g
[0036] Peptone 10g
[0037] NaCl 5g
[0038] Agar 20g
[0039] water 1000ml
[0040] PH7.4~7.6
[0041] Potato Medium:
[0042] Potatoes 200g
[0043] Glucose 20g
[0044] Agar 20g
[0045] water 1000ml
[0046] MRS medium: casein peptone: 10g
[0047] Beef extract: 10g
[0048] Yeast paste: 5g
[0049] Glucose: 5g
[0050] Sodium acetate: 5g
[0051] Diammonium hydrogen citrate: 2g
[0052] MgSO4 7H 2 O: 0.2g
[0053] MnSO4·H 2 O: 0.05g
[0054] K 2 HPO 4 ·H 2...
Embodiment 2
[0108] Embodiment 2 Preparation of high nitrogen source compound fertilizer
[0109] 1) Fertilizer preparation: buy commercially available compound mixed fertilization: nitrogen (N) 10%, phosphorus (P 2 o 5 ) 4%, Potassium (K 2 O) 6%, packing specification 50Kg / bag. The components are urea, potassium dihydrogen phosphate, and potassium chloride, and the indicated total nutrients are 20% (total of nitrogen, phosphorus, and potassium). The bacterium agent prepared in proportion is sprayed on the surface of the compound fertilizer granule with 8% (weight ratio), and after the fertilizer is dried, it is bagged to obtain the high inorganic nitrogen source biological bacterium compound fertilizer.
[0110] 2) Fertilizer effect test
[0111] The test location: Dingcheng Town, Ding'an County, Hainan Province. The soil quality of the test site is sandy loam with medium soil fertility. The previous crop is cucumber.
[0112] Experimental Variety: Shanghai Green Cabbage
[0113] Fe...
Embodiment 3
[0132] Embodiment 3 Preparation of high nitrogen source compound fertilizer
[0133] 1) Preparation of organic materials
[0134] Ingredients: dry chicken manure 60% (by weight), furfural residue 30%, soybean meal 10%
[0135] The above-mentioned material is crushed into particles of about 5mm, inserted into 1-2‰ (weight ratio) of the bacterial agent of the present invention, the moisture is adjusted to about 60%, piled into 0.8-1m high, 0.5-1m wide, solid fermentation for 7-10 days, After the fermentation is finished, it is dried and pulverized, and passed through a 30-mesh sieve to obtain an organic material.
[0136] 2), mixed puffing
[0137] The urea and the organic material are uniformly mixed according to the weight ratio of 15%, and are directly expanded with an expansion pressure of 2.3Mpa and a material temperature of 105°C for 35 seconds.
[0138] 3) Bacteria agent spraying
[0139] Inoculum preparation of the present invention is prepared to contain about 10 li...
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