Unlock instant, AI-driven research and patent intelligence for your innovation.
Simple and convenient method for inoculating cell for scaffold material of tissue engineering
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A scaffold material and cell technology, which can be used in tissue culture, biochemical equipment and methods, microorganisms, etc., and can solve problems such as complex operations
Inactive Publication Date: 2011-10-05
SICHUAN UNIV
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, these methods require the cooperation of special and expensive instruments, and the operation is more complicated.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0017] Human bone marrow stromal stem cells were seeded on TCP scaffolds for osteogenesis induction.
[0018] The bone marrow tissue collected clinically in the hospital was diluted with PBS solution, placed on the surface of an equal amount of Percoll liquid, and centrifuged at 3000rpm for 30 minutes. The milky white turbid mononuclear cell layer was collected for culture and expansion. After culturing to the second generation of cells, adjust the cell suspension to 5×10 6 / ml to prepare scaffolds for inoculation.
[0019] Prepare the TCP scaffold material with a size of 5mm×5mm×4mm, put the three scaffold materials into a 10ml syringe after being sterilized and dried. Aspirate 1ml of cell suspension, and reserve 1ml of air inside the syringe.
[0020] Block the opening of the syringe, withdraw the piston rod, so that the air inside the syringe reaches 3ml, and maintain for 5 seconds to slowly restore the pressure. Repeat this three times. Recover the excess cell suspens...
Embodiment 2
[0022] Human bone marrow stromal stem cells were seeded on TCP scaffolds for chondrogenic induction.
[0023] The bone marrow tissue collected in the hospital was diluted with PBS, placed on the surface of an equal amount of Percoll liquid, and centrifuged at 3000rpm for 30 minutes. The milky white turbid mononuclear cell layer was collected for culture and expansion. After culturing to the second generation of cells, adjust the cell suspension to 14×10 6 / ml to prepare scaffolds for inoculation.
[0024] Prepare the TCP scaffold material with a size of 5mm×5mm×4mm, put the three scaffold materials into a 10ml syringe after being sterilized and dried. Aspirate 1ml of cell suspension, and reserve 1ml of air inside the syringe.
[0025] Block the opening of the syringe, withdraw the piston rod, so that the air inside the syringe reaches 3ml, and maintain for 5 seconds to slowly restore the pressure. Repeat this three times. Recover the excess cell suspension and continue to...
Embodiment 3
[0027] MC3T3-E1 cells were seeded on PDLLA scaffolds for osteogenesis induction experiments.
[0028] Culture the MC3T3-E1 cell line in vitro, and adjust the cell suspension to 5×10 6 / ml to prepare scaffolds for inoculation.
[0029] The PDLLA scaffold material was prepared with a size of 10mm×10mm×5mm, and three scaffold materials were put into a 10ml syringe after being sterilized and dried. Aspirate 2ml of cell suspension, and reserve 1ml of air inside the syringe.
[0030] Block the opening of the syringe, withdraw the piston rod, so that the air inside the syringe reaches 3ml, and maintain for 5 seconds to slowly restore the pressure. Repeat this twice. Recover the excess cell suspension and continue to use it next time, move the inoculated cell-scaffold complex into a culture dish, let it stand in a cell culture incubator for 2 hours, and then add an osteogenic induction medium.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention provides a simple, convenient and effective method for realizing three-dimensional uniform inoculation of seed cells on a porous scaffold material of biological engineering. The method is characterized by comprising the following steps: using a clinically conventional sterilized syringe as an inoculating tool; putting a required biological scaffold material into the syringe from theback part thereof; sucking cell suspension, required for inoculating, with a corresponding number of cells; preserving a certain amount of air in the syringe; sealing the opening of the syringe; withdrawing the syringe; and enabling the cell suspension to permeate into air gaps of a porous material by using a formed negative pressure so as to realize the initial-stage uniform distribution and inoculation of the seed cells in the scaffold material. Compared with the method for realizing the initial-stage three-dimensional uniform inoculation of the scaffold material in the conventional tissue engineering practice, the method of the invention has the advantages of simplicity, convenience, easy implementation, and capability of achieving the effect of initial-stage three-dimensional uniform inoculation similar to special equipment without purchasing a special instrument with a large amount of funds. The inoculated cells are not damaged because the acting time of the negative pressure is short and the pressure is low.
Description
technical field [0001] The invention relates to a method for realizing initial two-dimensional uniform cell inoculation on a porous support material in in vitro research and clinical application of tissue engineering. Background technique [0002] In the practice of tissue engineering, some cells need to be cultured and expanded in a two-dimensional environment in vitro to reach a certain number, or to achieve directional differentiation of cells through intervention measures during the culture process, and then seeded on the scaffold material; Some are seeded directly onto the scaffold material after harvesting the seed cells. The cell seeding process plays a very important role in the initial two-dimensional spatial distribution of cells and the subsequent tissue engineering culture differentiation process. To achieve uniform distribution of cells in the scaffold material at the initial stage of seeding is one of the important evaluation indexes of cell seeding method. ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More