Florescent real-time quantitative PCR (Polymerase Chain Reaction) kit for detecting amplification of TERC (Telomerase RNA (Ribonucleic Acid) Component ) gene of esophageal cancer

Abstract

The invention provides a florescent real-time quantitative PCR kit for detecting the amplification of the TERC (Telomerase RNA (Ribonucleic Acid) Component) gene of the esophageal cancer, wherein the reference sequence of the TERC gene is as follows: NR_001566.1. The florescent real-time quantitative PCR kit comprises the following primers: (1) a forward primer for the amplification of the TERC gene: 5'-AACCCTAACTGAGAAGGGCG-3', (2) a reverse primer for the amplification of the TERC gene: 5'-TAGAATGAACGGTGGAAGGC-3', (3) a forward primer for the reference gene GAPDH (Glyceraldehyde-Phosphate Dehydrogenase):5'-CCAGGTGGTCTCCTCTGACTT-3', and (4) a reverse primer for the reference gene GAPDH: 5'-GTTGCTGTAGCCAAATTCGTTGT-3'. The florescent real-time quantitative PCR kit provided by the invention is used for detecting rRNA (ribosomal RNA) of the TERC gene in an esophageal tissue specimen to determine whether the TERC gene is amplified or not. Compared with a method for detecting the amplification of the TERC gene by an FISH (Fluorescence In Situ Hybridization) technology, the method provided by the invention has the advantages of short detection period and reduced cost.

Description

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AI Technical Summary

Benefits of technology

This patented device can be used to quickly measure how many times someone's DNA gets copied by another person or something else that may affect their health. It uses special chemical substances called lanthanum chloride (LaCl3) which are added during testing. By measuring this amount over time it helps determine if there have been any changes made on other genes related to eating behavior such as pancreatic enzyme beta levels.

Problems solved by technology

This patent describes different ways to identify specific types of lung cancer called esophogeal cancer and non-small cell lung cancer (NSCLCs). These methods involve analyzing DNA sequences derived from certain parts of the body's organs like blood vessels and airways, specifically including those associated with estrogenic esophagitis and gastrointestinal tract cancer. Overall, they provide valuable tools for understanding how progressed stages of esoquent stomach cancer develop into highly aggressive forms such as locally recurrent mesothelioma, perforation, and late stage disease.

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