Application of grve gene conferring resistance to Verticillium wilt in plants

A verticillium wilt and gene technology, applied in the field of plant genetic engineering, can solve the problems of long growth period, low yield, late maturity, etc., and achieve the effects of reducing disease index, good resistance effect, and delaying the onset time

Inactive Publication Date: 2014-10-01
JIANGSU ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

Although sea-island cotton has good resistance, tall plants, and strong growth potential, it has a long growth period and small bolls. In most cotton areas in my country, it not only matures late, but also has low yields.

Method used

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  • Application of grve gene conferring resistance to Verticillium wilt in plants
  • Application of grve gene conferring resistance to Verticillium wilt in plants
  • Application of grve gene conferring resistance to Verticillium wilt in plants

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Embodiment Construction

[0046] The methods used in the following embodiments are conventional methods unless otherwise specified, and the primer sequences used are all synthesized by Shanghai Handsome Biotechnology Co., Ltd., and the stated percentages are all mass percentages. The gene in this experiment was derived from black peak grape (Vitis vinifera).

[0047] (1) Grape GrVe cloning and sequence analysis

[0048] Primers were designed according to a receptor-like protein gene (accession number: XM_002263197) published in the Heifeng grape genome

[0049] P1: 5'-ATGTATCGAATCCTTTGCTTC-3',

[0050] P2:5'-CTAACTTCTTCTTGCTCCATG-3'

[0051] To amplify the cDNA template of Heifeng grape, add 1 μl of cDNA template to the 25 μl reaction system, 5 nmol of each primer, 5 μl of 5×primeSTARbuffer (Mg 2+ plus) PCR buffer, 0.2mMdNTP, 1UprimeSTAR HS DNA Polymerase (TaKaRa company) for PCR amplification. The PCR amplification conditions were: 94°C 3', 94°C 45'', 56°C 45'', 72°C 3', cycle 36 times, and then e...

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Abstract

The invention relates to an application of a GrVe gene providing plant with verticillium wilt resistance. The invention belongs to the technical field of biology. A GrVe gene is a surface receptor protein gene obtained from a Heifeng grape variety. The gene comprises 12 LRR conserved domains, and a transmembrane domain at a C end. The verticillium wilt resistance of a GrVe transgenic expression plant is substantially improved. With the gene, incidence can be retarded, and disease index is greatly reduced. After 16, 19, and 27 days of pathogen inoculation, verticillium wilt resistances of transgenic lines against defoliating verticillium V991 are substantially increased.

Description

1. Technical field [0001] The invention provides an application of a GrVe gene endowing plants with Verticillium wilt resistance, relates to plant gene cloning and functional analysis, and belongs to the field of plant genetic engineering. It is used to improve plant disease resistance and other beneficial production traits through plant genetic engineering technology. 2. Background technology [0002] Verticillium dahliae is caused by Verticillium dahliae and Verticillium albo-atrum and is the most serious soil-borne fungal vascular system disease widely existing in various regions of the world. The pathogen exists in the soil for a long time in the form of microsclerotia, stimulated by root exudates to germinate, and the formed germ tubes infect the plant roots, grow in the intercellular or intracellular cells of the root cortex, and finally spread to the xylem and the whole plants (Veronese P et al., 2003). Verticillium dahliae is a non-obligate parasite with a wide ran...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/84A01H5/00
Inventor 常有宏唐娟张保龙刘廷利杨郁文陈天子
Owner JIANGSU ACAD OF AGRI SCI
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