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Group of SSR markers for differentiating millet varieties as well as application of markers

A technology for millet and varieties, applied in the field of SSR marking, can solve the problems of difficult to accurately distinguish differences, difficult and inaccurate identification of millet varieties, etc.

Active Publication Date: 2015-04-29
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the performance of morphological characters is closely related to the field environment, it is difficult to accurately distinguish the differences between different millet varieties (genotypes) by traditional methods, and these characters are limited by the planting season and must be planted in the field. observed
This has just caused the difficulty and inaccuracy of millet variety identification

Method used

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  • Group of SSR markers for differentiating millet varieties as well as application of markers
  • Group of SSR markers for differentiating millet varieties as well as application of markers
  • Group of SSR markers for differentiating millet varieties as well as application of markers

Examples

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Embodiment 1

[0044] Example 1. A set of primers and methods for distinguishing millet varieties

[0045] 1. A set of primers for distinguishing millet varieties

[0046] The present invention constructs a set of primers containing 10 primer pairs (as shown in Table 1), and the set of primers of the present invention can quickly distinguish different millet varieties.

[0047] Table 1. Sequence and technical parameters of the primer set

[0048]

[0049] 2. The method of distinguishing millet varieties with a set of primers

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PUM

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Abstract

The invention discloses a group of SSR markers for differentiating millet varieties as well as an application of the markers. The SSR markers for differentiating millet varieties consist of a prime pair 1, a prime pair 2, a prime pair 3, a prime pair 4, a prime pair 5, a prime pair 6, a prime pair 7, a prime pair 8, a prime pair 9 and a prime pair 10. Experiments verify that being verified in farm varieties and improved varieties originated from different regions in Chinese for many times, the complete primers can be used for accurately and effectively differentiating millet varieties.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a set of SSR markers for distinguishing millet varieties and their applications. Background technique [0002] Millet (Setaria italica (L.) Beauv.), also known as millet and millet, belongs to the Gramineae family and the genus Setaria. It is the main food crop in northern my country with rich nutritional value. Millet is an ancient crop that originated in my country. It has been used as the main cultivated crop for thousands of years to cultivate the civilization of northern my country and is known as the nurturing crop of the Chinese nation. Because of its strong drought resistance and high water use efficiency, millet is considered to be a strategic reserve crop to cope with the increasingly serious water shortage in the future, and it has an irreplaceable role in today's sustainable ecological agriculture construction. my country is the country of origin of millet, with th...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6858C12Q1/6895C12Q2600/13C12Q2600/156C12Q2535/131
Inventor 刁现民贾冠清智慧王春芳刘晓彤牛振刚柴杨
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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