A method for inducing neuroblastoma cells to differentiate into neurons

A neuroblastoma, neural stem cell technology, applied in the field of cell research, can solve the problems of long differentiation time and low neuron cell differentiation efficiency, and achieve the effect of inhibiting cell apoptosis
CN105176929BActive Publication Date: 2018-03-20SHANDONG QILU STEM CELL ENG
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANDONG QILU STEM CELL ENG
Publication Date
2018-03-20

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Abstract

The invention relates to cell transdifferentiation technology in the field of cell research, in particular to a method for inducing neuroblastoma cells to differentiate into neurons. Neuroblastoma cells were cultured with induction medium for 3-7 days to obtain neuronal cells; induction medium was obtained by adding retinoic acid to 10 μM to neural stem cell conditioned medium; DMEM / F12 basal medium, B27 , bFGF, EGF, heparin sodium, L-glutamine, and penicillin-streptomycin complete medium for culturing neural stem cells, cultured for 3 days and half of the complete medium was replaced, and the replaced culture medium was the neural stem cell conditioned medium. The method of the invention improves the differentiation efficiency, improves the maturity of the cells and the synapse growth of the neuron cells, and can inhibit the apoptosis in the SH-SY5Y cells treated with RA.
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Description

technical field

[0001] The invention relates to cell transdifferentiation technology in the field of cell research, in particular to a method for inducing neuroblastoma cells to differentiate into neurons. Background technique

[0002] It is well known that neurons differentiated from neuroblastoma SH-SY5Y cell line are commonly used cell models for neurobiological research, such as neurotoxicity research and in vitro neuronal tolerance to morphine [Constantinescu, JNeural Transm Suppl (2007)]. SH-SY5Y is a human-derived cell line that can expand continuously without differentiation under in vitro culture conditions. To differentiate SH-SY5Y cells into neurons, retinoic acid (RA) is usually used for induction. However, the ratio of neuronal cells differentiated from SH-SY5Y cells was very low, whether induced by RA alone or in combination with other compounds. RA alone induced only about 20% of SH-SY5Y cells to differentiate into neurons [Preis, CancerRes (1988)]. In addi...

Claims

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