Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for inducing neuroblastoma cells to differentiate into neurons

A neuroblastoma, neural stem cell technology, applied in the field of cell research, can solve the problems of long differentiation time and low neuron cell differentiation efficiency, and achieve the effect of inhibiting cell apoptosis

Active Publication Date: 2018-03-20
SHANDONG QILU STEM CELL ENG
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of low differentiation efficiency and long differentiation time of neuroblastoma cells differentiated from neuroblastoma cells in the prior art, the present invention provides an induced neuroblastoma cell with a differentiation effect greater than 80% and a short differentiation time Methods of Differentiation into Neurons

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for inducing neuroblastoma cells to differentiate into neurons
  • A method for inducing neuroblastoma cells to differentiate into neurons
  • A method for inducing neuroblastoma cells to differentiate into neurons

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Preparation of neural stem cell conditioned medium

[0025] Human neural stem cells were purchased from Lonza Company and cultured in T-75 culture flasks with 15 ml of complete medium. Complete medium containing DMEM / F12 basal medium (Invitrogen), human recombinant epidermal growth factor (EGF, 20ng / mL) and basic fibroblast growth factor (bFGF, 20ng / mL) (R&D), B27 (serum replacement , 1:50, Invitrogen), heparin (5 μg / mL, Sigma), L-glutamine 2mM, and penicillin and streptomycin (1:100, Invitrogen). at 37°C with 5% CO 2 Incubate in a humidified incubator, and replace 50% of the medium with new complete medium every 3 days. When the neurospheres grow to a diameter of about 1 mm, they are cut and passaged under a microscope. When the culture medium is replaced each time, the old culture medium replaced is the neural stem cell conditioned medium. The conditioned medium was collected and filtered through a 0.22 μm pore size filter (Millipore), then centrifuged at 1000...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to cell transdifferentiation technology in the field of cell research, in particular to a method for inducing neuroblastoma cells to differentiate into neurons. Neuroblastoma cells were cultured with induction medium for 3-7 days to obtain neuronal cells; induction medium was obtained by adding retinoic acid to 10 μM to neural stem cell conditioned medium; DMEM / F12 basal medium, B27 , bFGF, EGF, heparin sodium, L-glutamine, and penicillin-streptomycin complete medium for culturing neural stem cells, cultured for 3 days and half of the complete medium was replaced, and the replaced culture medium was the neural stem cell conditioned medium. The method of the invention improves the differentiation efficiency, improves the maturity of the cells and the synapse growth of the neuron cells, and can inhibit the apoptosis in the SH-SY5Y cells treated with RA.

Description

technical field [0001] The invention relates to cell transdifferentiation technology in the field of cell research, in particular to a method for inducing neuroblastoma cells to differentiate into neurons. Background technique [0002] It is well known that neurons differentiated from neuroblastoma SH-SY5Y cell line are commonly used cell models for neurobiological research, such as neurotoxicity research and in vitro neuronal tolerance to morphine [Constantinescu, JNeural Transm Suppl (2007)]. SH-SY5Y is a human-derived cell line that can expand continuously without differentiation under in vitro culture conditions. To differentiate SH-SY5Y cells into neurons, retinoic acid (RA) is usually used for induction. However, the ratio of neuronal cells differentiated from SH-SY5Y cells was very low, whether induced by RA alone or in combination with other compounds. RA alone induced only about 20% of SH-SY5Y cells to differentiate into neurons [Preis, CancerRes (1988)]. In addi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0793
Inventor 刘小盾杨洪娜曲廷瑜
Owner SHANDONG QILU STEM CELL ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com