14 results about "Cell Transdifferentiation" patented technology

The invention discloses a method for transdifferentiating tail fin cells of erythroculter ilishaeformis into fat and muscle cells and application, the method is based on 5-Azacytidine (azacytidine) to promote mesenchymal stem cells to be differentiated into muscle cells, and other small molecule compounds are added on the basis of adding the 5-Azacytidine to transdifferentiate the tail fin cells of erythroculter ilishaeformis into muscles. The method is realized through an in-vitro cell reprogramming technology, fish individuals do not need to be sacrificed, and the destructive influence of traditional muscle or fat sampling on fish bodies is avoided; a single-step induction system does not need multi-factor sequential treatment, a high-purity target cell population can be obtained only in 5-7 days at least, the standardization degree of the operation process is high, the stability among batches is excellent, specific cells can be continuously and stably produced, and a safe and reliable technical basis is provided for sustainable cell culture fish meat production.

The application discloses a kind of small molecule compound combination and the method for preparing vascular endothelial cell using the small molecule compound combination induced differentiation cell.The method is directed to induce differentiated cell, and finally prepare vascular endothelial cell;The directional induction includes inhibiting the activity of lysine deacetylase, inhibiting the signal path of TGF-β, inhibiting the activity of DNA methyltransferase (DNMT), activating the signal path of WNT / β-catenin and activating the signal path of cAMP.The present application can be converted into vascular endothelial cell by small molecule compound combination induction, and each step can realize accurate quality control, which is convenient for standardized operation and large-scale production.The method provided by the present application has wide donor sources, and the patient himself can be used as a donor, so that the vascular endothelial cells required for basic research, clinical treatment or tissue engineering production can be obtained in a short time.

The invention relates to the field of biological medicines, in particular to application of a hepatocyte nuclear factor 1 alpha (HNF1 alpha) in promoting beta cell regeneration and preventing and treating diabetes. Specifically, by regulating and controlling the expression of an HNF1alpha coding gene and the activity of HNF1alpha, pancreas islet alpha cells can be promoted to be transdifferentiated into beta cells to achieve beta cell regeneration, the functions of pancreas islet beta cells are reserved, the occurrence of diabetes mellitus is delayed, and a new medical approach is provided for prevention and treatment of diabetes mellitus.

The application provides a method for transdifferentiation of fibroblasts into hair papilla-like cells and application thereof, and relates to the technical field of biotechnology. The transdifferentiation method comprises inducing fibroblasts by using Peficitinib, so that the fibroblasts are transdifferentiated into hair papilla-like cells. A large number of hair papilla-like cells with hair induction capacity can be obtained in vitro by the method, and the problem that primary separated hair papilla cells are insufficient for developing alternative therapies for treating alopecia in the prior art is solved.

A composition and method for transdifferentiating non-neuronal cells into neurons. Disclosed is a composition for inducing cell transdifferentiation, comprising a Myosin inhibitor, and an isoxazole compound and / or its derivatives. Also disclosed is a method for inducing non-neuronal cells to transdifferentiate into neurons, comprising: culturing non-neuronal cells in an induction culture medium comprising a Myosin inhibitor, and then culturing them in a maturation culture medium comprising a Myosin inhibitor and an isoxazole compound and / or its derivatives until mature neurons are obtained. Also disclosed is a use of the composition in inducing non-neuronal cells to transdifferentiate into neurons, and a method for transdifferentiating non-neuronal cells in a subject into neurons, comprising administering an effective amount of a Myosin inhibitor and an isoxazole compound and / or its derivatives to the subject. The method of the present application is simple, requiring only two simple small molecule combination treatments, without the need for regulation by overexpression of specific genes, and can be performed efficiently both in vivo and in vitro, thereby achieving simple and efficient neuronal regeneration.

The invention discloses a method for constructing a vascularized tumor microenvironment based on a genetic code recoding technology, which comprises the following steps of: constructing biological orthogonal translation systems PiggyBac-(7sk-tRNAPyl) x4-EF1a-MmPylRS and PiggyBac-ETV2122 *, transfecting the biological orthogonal translation systems and transposase plasmids into a liver cancer cell line, and screening a Huh-7 cell line which stably expresses the biological orthogonal translation systems; and carrying out balling culture on the obtained Huh-7 cell line, and adding NAEK in the culture process to induce partial Huh-7 cells to be transdifferentiated into endothelial cells, so as to obtain vascularized liver cancer cell balls and realize the construction of a tumor microenvironment. The genetic code recoding technology is used for regulating and controlling formation of the functional vascular tumor microenvironment, and the method has the advantages of being simple and easy to operate and high in repeatability and universality. The technology is a near-physiological protein translation regulation and control means, the regulation and control speed is high, protein translation regulation and control can be rapidly started or stopped, a traditional two-dimensional culture mode can be replaced, multi-cell interaction is achieved, and the biomimetic property is better.

The invention relates to the field of preparation of immune cells, in particular to preparation and application of macrophages. The invention provides a method for preparing macrophages, and relates to transdifferentiation of B cells into macrophages. According to the method disclosed by the invention, a B cell is taken as a starting cell, engineering transformation is carried out, an exogenous transcription factor is introduced, and the B cell is induced to generate cell reprogramming to prepare the macrophage. The method provided by the invention can also be used for preparing a modified macrophage (such as a CAR modified macrophage). By utilizing the preparation method provided by the invention, the macrophages can be rapidly and efficiently prepared, and the preparation period of the macrophages is greatly shortened. The macrophage produced by the invention has the due functions of normal macrophages, does not have multiplication capacity, still maintains the functions of the macrophages after ray irradiation, has safety in clinical use, and can be applied to clinical treatment.

There is provided a method of transdifferentiating a non-neuronal cell of a mammal into neurons, comprising: providing a negative modulator capable of reducing expression of a negative regulatory gene, the negative regulatory gene comprising RCOR1, RCOR2, RCOR3, Sin3a, Sin3b, HDAC1, HDAC2, KDM1A, PHF21A, BAF53a, G9a, USP14, HuR, BrG1, EZH2, CDYL, or HMG20B, and / or providing a positive modulator capable of increasing expression of a positive regulatory gene, the positive regulatory gene comprising DPYSL2, BAF45b, SCF, HuB, HuC, HuD, CYP1B1, or BTRC, the negative regulatory gene comprising RCOR1, RCOR2, RCOR3, Sin3a, Sin3b, an effective amount of the negative regulator or the positive regulator is contacted with the non-neuronal cells, so that the non-neuronal animal cells can be induced to be transdifferentiated into neurons.

The invention discloses application of Crx, Nrl and Otx2 in inducing cells to be transdifferentiated into photoreceptor-like cells and a method for preparing the photoreceptor-like cells, the method comprises the step of expressing induction genes in starting cells, and the induction genes are Crx, Nrl and Otx2. Inducing genes Crx, Nrl and Otx2 are expressed in fibroblasts, so that the fibroblasts are transdifferentiated into photoreceptor-like cells, and the photoreceptor-like cells can be automatically migrated and integrated into the retina of a host after being transplanted into the subretinal space of a mouse, so that the damaged retina is repaired. Since a large number of fibroblasts are present in the skin, this makes it possible to treat retinal injury by inducing transdifferentiation of fibroblasts into photoreceptor-like cells on a large scale.

The invention discloses application of an epigenetic modification regulation and control substance in inducing transdifferentiation of embryonic stem cells to original endoderm stem cells. The epigenetic modification regulation and control substance is a substance for reducing the expression level of H3K27me3 in the embryonic stem cells. By regulating epigenetic modification, an in-vitro culture system capable of inducing efficient transdifferentiation of ESCs to PrESC is constructed, and functional verification shows that a PrESC cell line is successfully constructed.

The invention discloses a culture solution and a method for transdifferentiating airway epithelial cells into lung progenitor cells. In particular to a culture solution for transdifferentiating airway epithelial cells into lung progenitor cells. The culture solution is prepared by adding FGF10, IGF1, KGF, a Wnt agonist, retinoic acid, BMP4, a PRMT5 inhibitor, a p38 kinase inhibitor and a Smoothed agonist on the basis of a basic culture medium. A large number of SOX < 2 + > lung epithelial cells are obtained by separating mouse atmospheric duct epithelial cells and performing 3D culture amplification in vitro, and then conditions capable of obtaining far-end SOX < 9 + > epithelial cells are screened by optimizing morphogenetic factors and regulatory molecules of various signal channels in a culture system. By adopting the culture solution and the method disclosed by the invention, the near-end SOX < 2 + > epithelial cells can be efficiently transdifferentiated into the far-end SOX < 9 + > epithelial cells.

The present invention relates to a method of treating RAS-dependent cancer in a subject by use of a farnesyltransferase inhibitor. Also described are compositions comprising a farnesyltransferase inhibitor; and an agent that increases the amount of geranylgeranyl pyrophosphate (GGPP). Lastly, the use of farnesyltransferase inhibitor for transdifferentiating a cell into a fibroblast-like stage is described herein.

The invention particularly discloses a method for inducing transdifferentiation of paving cells in mature leaves of nicotiana benthamiana into glandular hair cells, which comprises the following steps: preparing an agrobacterium infection solution, and agrobacterium is selected from one or more of a GV3101 strain, a C58C1 strain or a K599 strain; carrying out infection treatment on completely expanded mature leaves of the nicotiana benthamiana by using an agrobacterium infection solution; and after infection, the nicotiana benthamiana is cultured, and the paving cells of the leaves are induced to be transdifferentiated into glandular hair cells. The method is easy to operate and good in repeatability, stable genetic transformation and strict sterile conditions are not needed, the paving cells of the Bensi tobacco leaves can be induced to be transdifferentiated into the glandular hair cells, and increase of the number of the glandular hair cells is promoted.