8 results about "Cell Transdifferentiation" patented technology

The application discloses a kind of small molecule compound combination and the method for preparing vascular endothelial cell using the small molecule compound combination induced differentiation cell.The method is directed to induce differentiated cell, and finally prepare vascular endothelial cell;The directional induction includes inhibiting the activity of lysine deacetylase, inhibiting the signal path of TGF-β, inhibiting the activity of DNA methyltransferase (DNMT), activating the signal path of WNT / β-catenin and activating the signal path of cAMP.The present application can be converted into vascular endothelial cell by small molecule compound combination induction, and each step can realize accurate quality control, which is convenient for standardized operation and large-scale production.The method provided by the present application has wide donor sources, and the patient himself can be used as a donor, so that the vascular endothelial cells required for basic research, clinical treatment or tissue engineering production can be obtained in a short time.

The invention relates to the field of biological medicines, in particular to application of a hepatocyte nuclear factor 1 alpha (HNF1 alpha) in promoting beta cell regeneration and preventing and treating diabetes. Specifically, by regulating and controlling the expression of an HNF1alpha coding gene and the activity of HNF1alpha, pancreas islet alpha cells can be promoted to be transdifferentiated into beta cells to achieve beta cell regeneration, the functions of pancreas islet beta cells are reserved, the occurrence of diabetes mellitus is delayed, and a new medical approach is provided for prevention and treatment of diabetes mellitus.

The application provides a method for transdifferentiation of fibroblasts into hair papilla-like cells and application thereof, and relates to the technical field of biotechnology. The transdifferentiation method comprises inducing fibroblasts by using Peficitinib, so that the fibroblasts are transdifferentiated into hair papilla-like cells. A large number of hair papilla-like cells with hair induction capacity can be obtained in vitro by the method, and the problem that primary separated hair papilla cells are insufficient for developing alternative therapies for treating alopecia in the prior art is solved.

The invention discloses a method for constructing a vascularized tumor microenvironment based on a genetic code recoding technology, which comprises the following steps of: constructing biological orthogonal translation systems PiggyBac-(7sk-tRNAPyl) x4-EF1a-MmPylRS and PiggyBac-ETV2122 *, transfecting the biological orthogonal translation systems and transposase plasmids into a liver cancer cell line, and screening a Huh-7 cell line which stably expresses the biological orthogonal translation systems; and carrying out balling culture on the obtained Huh-7 cell line, and adding NAEK in the culture process to induce partial Huh-7 cells to be transdifferentiated into endothelial cells, so as to obtain vascularized liver cancer cell balls and realize the construction of a tumor microenvironment. The genetic code recoding technology is used for regulating and controlling formation of the functional vascular tumor microenvironment, and the method has the advantages of being simple and easy to operate and high in repeatability and universality. The technology is a near-physiological protein translation regulation and control means, the regulation and control speed is high, protein translation regulation and control can be rapidly started or stopped, a traditional two-dimensional culture mode can be replaced, multi-cell interaction is achieved, and the biomimetic property is better.

The invention discloses application of an epigenetic modification regulation and control substance in inducing transdifferentiation of embryonic stem cells to original endoderm stem cells. The epigenetic modification regulation and control substance is a substance for reducing the expression level of H3K27me3 in the embryonic stem cells. By regulating epigenetic modification, an in-vitro culture system capable of inducing efficient transdifferentiation of ESCs to PrESC is constructed, and functional verification shows that a PrESC cell line is successfully constructed.

The present invention relates to a method of treating RAS-dependent cancer in a subject by use of a farnesyltransferase inhibitor. Also described are compositions comprising a farnesyltransferase inhibitor; and an agent that increases the amount of geranylgeranyl pyrophosphate (GGPP). Lastly, the use of farnesyltransferase inhibitor for transdifferentiating a cell into a fibroblast-like stage is described herein.

The invention particularly discloses a method for inducing transdifferentiation of paving cells in mature leaves of nicotiana benthamiana into glandular hair cells, which comprises the following steps: preparing an agrobacterium infection solution, and agrobacterium is selected from one or more of a GV3101 strain, a C58C1 strain or a K599 strain; carrying out infection treatment on completely expanded mature leaves of the nicotiana benthamiana by using an agrobacterium infection solution; and after infection, the nicotiana benthamiana is cultured, and the paving cells of the leaves are induced to be transdifferentiated into glandular hair cells. The method is easy to operate and good in repeatability, stable genetic transformation and strict sterile conditions are not needed, the paving cells of the Bensi tobacco leaves can be induced to be transdifferentiated into the glandular hair cells, and increase of the number of the glandular hair cells is promoted.