Method for integrating multi-omics data to presume key protein kinases in cell transdifferentiation

A protein kinase and omics data technology, applied in the field of integrating multi-omics data to speculate key protein kinases in cell transdifferentiation, can solve problems that restrict the research and development of cell transdifferentiation mechanism, high cost of screening models, and lack of clear purpose , to achieve the effect of avoiding large-scale screening work, shortening the research and development cycle, and improving accuracy

Inactive Publication Date: 2019-03-29
EZHOU INST OF IND TECH HUAZHONG UNIV OF SCI & TECH +1
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Problems solved by technology

[0004] At present, the research on the mechanism of cell transdifferentiation usually adopts the method of large-scale screening. This screening mode is expensive, has no clear purpose, takes a long time, and has a very low success rate. It has no obvious practical significance and help for many biological experiments. This severely restricts the research and development of the mechanism of cell transdifferentiation

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  • Method for integrating multi-omics data to presume key protein kinases in cell transdifferentiation
  • Method for integrating multi-omics data to presume key protein kinases in cell transdifferentiation
  • Method for integrating multi-omics data to presume key protein kinases in cell transdifferentiation

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Embodiment 1

[0029] This example discloses in detail the method for inferring key protein kinases in cell transdifferentiation by integrating multi-omics data. The specific process is as follows: figure 1 As shown, this method is based on the data of omics detection, collects various cell samples in the process of cell differentiation, and through the significant differences in the expression, activity, and regulation of protein kinases in multiple samples, the cell transdifferentiation is obtained comprehensively. key protein kinases.

[0030] The detailed process is as follows:

[0031] (1) Prepare cell samples, divide cell samples evenly into transcriptome and protein phosphorylation group, conduct omics detection for each group, and obtain corresponding raw data;

[0032] Wherein, the cell samples include source cells that have not been transformed, transformed cells that have been successfully transformed, and initial state cells, intermediate state cells, mature state cells, and ste...

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Abstract

The invention discloses a method for integrating multi-omics data to presume key protein kinases in cell transdifferentiation. The method comprises the steps of: preparing a cell sample during whole cell transdifferentiation, dividing the cell sample into a transcriptome group and a protein phosphorylation group, and conducting omics tests separately to obtain corresponding original data of each group; pre-processing the original data, screening the protein kinases expressing significant differences through a transcriptome, and screening the protein kinases with activity significant differences and regulation significant differences through a phosphoproteome; and integrating all protein kinases with significant differences, and conducting comprehensive measure to obtain key protein kinases. The method of the invention can provide small-scale candidate kinases for the research of cell transdifferentiation mechanism, provide a guiding direction for the research of the cell transdifferentiation mechanism, greatly narrow the screening range, improve the research efficiency and the accuracy of the experiment, shorten the research and development cycle, increase the success rate of experiments, and reduce the cost of research on cell transdifferentiation mechanisms.

Description

technical field [0001] The invention relates to the technical field of cell biology, and specifically refers to a method for inferring key protein kinases in cell transdifferentiation by integrating multi-omics data. Background technique [0002] With the development and improvement of technologies such as somatic cell nuclear transfer, cell fusion and cell transdifferentiation, it has been proved that the nucleus of most animal cells is totipotent, and the fate of highly differentiated cells is not irreversible. Specific cell types can be transdifferentiated into another cell type under the induction of appropriate reprogramming transcription factors. Transdifferentiation technology provides a new idea for human cell disease models and molecular replacement therapy. In recent years, breakthroughs have been made in cell reprogramming. Cell reprogramming factors have been screened for the first time to obtain induced pluripotent stem cells, which proves that transcription fac...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/12
CPCC12N9/1205
Inventor 薛宇黄鹏羽林少峰袁阳阳
Owner EZHOU INST OF IND TECH HUAZHONG UNIV OF SCI & TECH
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