Stem cell directional differentiation and cell transdifferentiation method

A directional differentiation, stem cell technology, applied in the field of stem cell research, can solve the problem of no in-depth research on directional differentiation of stem cells

Inactive Publication Date: 2021-08-13
杭州憶盛医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the existing defects and provide a method for directional differentiation of stem cells and cell transdifferentiation to solve the problem of no in-depth research on directional differentiation of stem cells raised in the above-mentioned background technology

Method used

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Embodiment 1

[0026] A method for directed differentiation of stem cells and cell transdifferentiation, comprising the following steps:

[0027] Step 1: Extract embryonic tissue from the placenta of young healthy pregnant women, place the embryonic tissue in a culture dish filled with cell culture medium and let it stand for 15 minutes. , placing the maternal cells in a petri dish to continue culturing;

[0028] Step 2: After the division of the mother cells, use a microscope to observe the division state of the mother cells, and extract the second-generation daughter cells from the division cells, digest them with trypsin, and use a medium containing high-sugar DMEM, non-essential amino acids, and 10% fetal bovine serum , do not contain leukemia inhibitory factor to make a single cell suspension, inoculate about 600 cells / 3L on the inner surface of the lid of the culture dish, turn the lid over to form a hanging drop, and culture it in an incubator at 37°C for 3 days to obtain ES Hanging ...

Embodiment 2

[0034] A method for directed differentiation of stem cells and cell transdifferentiation, comprising the following steps:

[0035] Step 1: Extract embryonic tissue from the placenta of young healthy pregnant women, place the embryonic tissue in a culture dish filled with cell culture medium and let it stand for 15 minutes. , placing the maternal cells in a petri dish to continue culturing;

[0036] Step 2: After the division of the mother cells, use a microscope to observe the division state of the mother cells, and extract the second-generation daughter cells from the division cells, digest them with trypsin, and use a medium containing high-sugar DMEM, non-essential amino acids, and 10% fetal bovine serum , do not contain leukemia inhibitory factor to make a single cell suspension, inoculate about 600 cells / 3L on the inner surface of the lid of the culture dish, turn the lid over to form a hanging drop, and culture it in an incubator at 37°C for 3 days to obtain ES Hanging ...

Embodiment 3

[0042] A method for directed differentiation of stem cells and cell transdifferentiation, comprising the following steps:

[0043] Step 1: Extract embryonic tissue from the placenta of young healthy pregnant women, place the embryonic tissue in a culture dish filled with cell culture medium and let it stand for 15 minutes. , placing the maternal cells in a petri dish to continue culturing;

[0044] Step 2: After the division of the mother cells, use a microscope to observe the division state of the mother cells, and extract the second-generation daughter cells from the division cells, digest them with trypsin, and use a medium containing high-sugar DMEM, non-essential amino acids, and 10% fetal bovine serum , do not contain leukemia inhibitory factor to make a single cell suspension, inoculate about 600 cells / 3L on the inner surface of the lid of the culture dish, turn the lid over to form a hanging drop, and culture it in an incubator at 37°C for 3 days to obtain ES Hanging ...

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Abstract

The invention discloses a stem cell directional differentiation and cell transdifferentiation method. The method comprises the following steps of: step 1, extracting embryonic tissues from a placenta of a young healthy pregnant woman, placing the embryonic tissues in a culture dish filled with a cell culture solution, standing for 15min, taking out the embryonic tissues after standing, cutting the embryonic tissues into pieces to obtain embryonic parent cells, and placing the parent cells in the culture dish for continuous culture. According to the method, different differentiation stimulators are adopted to perform different differentiation induction on embryonic stem cells, and directional differentiation results of the different stimulators can be explored through embryonic stem cell differentiation results, so that the directional differentiation results of the embryonic stem cells under the action of the stimulators can be known, in-vitro differentiation conditions of the embryonic stem cells can be deeply explored, the directional differentiation results and transdifferentiation results of the embryonic stem cells can be known, and scientific researchers can know specific differentiation influence factor conditions of the embryonic stem cells conveniently.

Description

technical field [0001] The invention belongs to the technical field of stem cell research, and in particular relates to a method for directed differentiation and cell transdifferentiation of stem cells. Background technique [0002] At present, most biologists and medical scientists believe that stem cells are a type of cells that come from embryos, fetuses or adults with unlimited self-renewal, proliferation and differentiation capabilities under certain conditions, and can produce phenotypes and genotypes that are exactly the same as themselves. Daughter cells can also produce specialized cells that make up body tissues and organs, and can also differentiate into progenitor cells. [0003] Cell differentiation refers to the process in which cells from the same source gradually produce cell groups with different morphological structures and functional characteristics. The result is that cells are different in space, and the same cell is different from its previous state in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/078
CPCC12N5/0634C12N5/0641C12N2501/14C12N2501/22C12N2506/02
Inventor 彭伟群曹小伍曹得明邵草品雷铭轩
Owner 杭州憶盛医疗科技有限公司
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