Pretreatment method for pteridophyte sporangium observing material

A fern, pre-treatment technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of incomplete, unclear, and unsightly morphological structure of fern sporangia, and achieve a clear structure. Visible, morphologically intact, contrast-enhancing effects

Inactive Publication Date: 2015-12-30
SHANGHAI CHENSHAN BOTANICAL GARDEN
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This technology uses chemical substances called chlorine dioxide (CIO2) that can react with certain types of bacterial cells or other organisms found inside them. These chemistry help break down any harmful things from these germs into smaller particles for easier removal by filtration techniques like filter paper.

Problems solved by technology

This patented describes how different types of plants called Fennelmaceae (female) form colonies that contain tiny seedings made from certain parts or tissue found at various locations throughout the world' s barked areas such as China, Japan, India, Nepalese, etc., where they were introduced during periods when other species had gone extinct ago. These plantlets may include roots, stems, branches, rootstock, stembs, foliage, litterels, nuts/seeds, berries, wormstops, pollen grains, pterisks, conifers, carrots, rhizomes, dendritic tuberosols, galls, sea urchins, nectars, oyster shellfish, fish eggs, caterpillar fungus, citrus fruit trees, tea tree oil palms, sugarcane, rice crops, wheat germs, peanut butters, chlorella grass, among others.

Method used

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  • Pretreatment method for pteridophyte sporangium observing material
  • Pretreatment method for pteridophyte sporangium observing material
  • Pretreatment method for pteridophyte sporangium observing material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In mid-October, when the spores of fern (Pteridiumaquilinumvar. fern sporangia figure 1 .

[0022] Place the fern sporangia on a glass slide to make water-mounted slides (without covering the cover glass), add 2 drops of sodium hypochlorite to the center to completely submerge the fern sporangia. Since sodium hypochlorite is oxidizing, the material can be seen at this time The color gradually becomes darker, and then gradually becomes lighter. At this time, you can continue to observe it under the stereoscope.

[0023] Continue to observe for 15 minutes, and immediately add 2 drops of distilled water to dilute sodium hypochlorite to fern sporangia before the color of the ring fades, so as to avoid excessive fading of fern sporangia.

[0024] After diluting the sodium hypochlorite with distilled water, blot the sodium hypochlorite solution on the glass slide with filter paper.

[0025] Add 2 drops of distilled water dropwise, and then absorb it with filter paper. When...

Embodiment 2

[0028] In mid-October, when the spores of Grammitis dorsipila (Latin name Grammitis dorsipila) mature, the spores in the sporangia of Grammitis dorsipila are all empty, and then the fully mature sporangia are collected as observation treatment Material, untreated Brachypodium graminearum sporangia such as image 3 .

[0029] Place the brachypodium sporangia on the glass slide to make water-mounted slides (without cover glass), add 2 drops of sodium hypochlorite to the center, and completely submerge the brachypodium sporangia, because sodium hypochlorite has the ability to oxidize At this time, it can be seen that the color of the sporangia of Brachypedia graminearum gradually becomes darker, and then gradually becomes lighter. At this time, you can continue to observe it under the stereoscope.

[0030] Continue to observe for 12 minutes, and immediately add 2 drops of distilled water to dilute sodium hypochlorite to the brachypodium sporangia before the color of the ring fad...

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Abstract

The invention provides a pretreatment method for a pteridophyte sporangium observing material, relates to a method for treating a whole filicopsida sporangium observing material, and belongs to the field of plant morphology and plant photomicrography. Sodium hypochlorite with redox properties acts on pteridophyte sporangium, so that the pteridophyte sporangium is softened, recovers and fades, impurities are eliminated, the observed pteridophyte sporangium is integral in form and clear and visible in structure, annulus color contrast is enhanced, and research ability of the pteridophyte sporangium and attractiveness of photos are improved greatly.

Description

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Claims

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Application Information

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Owner SHANGHAI CHENSHAN BOTANICAL GARDEN
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